Restriction map for
pFN21AA0291
(Linear) (Six-base) MAPSORT of: KIAA0291.seq Check: 3805 from: 1 to: 3033
KIAA0291.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2901 3033
Size: 2901 132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1102 1627 1699 1861 2527 3033
Size: 40 1062 525 72 162 666 506
Fragments arranged by size:
1062 666 525 506 162 72 40
AhdI GACnn_n'nnGTC
Cuts at: 0 938 3033
Size: 938 2095
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1030 1235 3033
Size: 1030 205 1798
Fragments arranged by size:
1798 1030 205
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1321 1353 3033
Size: 1321 32 1680
Fragments arranged by size:
1680 1321 32
AlwNI CAG_nnn'CTG
Cuts at: 0 175 291 504 1113 1470 2598 3033
Size: 175 116 213 609 357 1128 435
Fragments arranged by size:
1128 609 435 357 213 175 116
ApaI G_GGCC'C
Cuts at: 0 196 1810 2010 2448 3033
Size: 196 1614 200 438 585
Fragments arranged by size:
1614 585 438 200 196
ApaLI G'TGCA_C
Cuts at: 0 2089 3033
Size: 2089 944
AvaI C'yCGr_G
Cuts at: 0 181 459 1053 1479 1803 2568 2836 3033
Size: 181 278 594 426 324 765 268 197
Fragments arranged by size:
765 594 426 324 278 268 197 181
BamHI G'GATC_C
Cuts at: 0 607 3033
Size: 607 2426
BanI G'GyrC_C
Cuts at: 0 796 1057 1813 2519 2832 3033
Size: 796 261 756 706 313 201
Fragments arranged by size:
796 756 706 313 261 201
BanII G_rGCy'C
Cuts at: 0 196 460 767 1251 1430 1460 1810 2010
Size: 196 264 307 484 179 30 350 200
Cuts at: 2010 2448 3033
Size: 438 585
Fragments arranged by size:
585 484 438 350 307 264 200 196
179 30
BbeI G_GCGC'C
Cuts at: 0 1817 2523 2836 3033
Size: 1817 706 313 197
Fragments arranged by size:
1817 706 313 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 595 697 904 2560 3033
Size: 595 102 207 1656 473
Fragments arranged by size:
1656 595 473 207 102
BbvCI CC'TCA_GC
Cuts at: 0 1139 3033
Size: 1139 1894
BglI GCCn_nnn'nGGC
Cuts at: 0 59 1010 1609 1618 3033
Size: 59 951 599 9 1415
Fragments arranged by size:
1415 951 599 59 9
BglII A'GATC_T
Cuts at: 0 1049 2159 3033
Size: 1049 1110 874
Fragments arranged by size:
1110 1049 874
BlpI GC'TnA_GC
Cuts at: 0 2906 3033
Size: 2906 127
Bme1580I G_kGCm'C
Cuts at: 0 196 397 523 799 817 1060 1810 2010
Size: 196 201 126 276 18 243 750 200
Cuts at: 2010 2093 2448 3033
Size: 83 355 585
Fragments arranged by size:
750 585 355 276 243 201 200 196
126 83 18
BmgBI CAC'GTC
Cuts at: 0 2112 3033
Size: 2112 921
BmrI ACTGGGnnnn_n'
Cuts at: 0 83 3033
Size: 83 2950
BmtI G_CTAG'C
Cuts at: 0 110 3033
Size: 110 2923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2178 3033
Size: 2178 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 388 1324 1396 1438 1444 1798 1900
Size: 33 355 936 72 42 6 354 102
Cuts at: 1900 1933 2035 2062 2260 2482 2545 2557 2665
Size: 33 102 27 198 222 63 12 108
Cuts at: 2665 3033
Size: 368
Fragments arranged by size:
936 368 355 354 222 198 108 102
102 72 63 42 33 33 27 12
6
Bpu10I CC'TnA_GC
Cuts at: 0 1139 2440 2683 3033
Size: 1139 1301 243 350
Fragments arranged by size:
1301 1139 350 243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 2134 2716 3033
Size: 1180 954 582 317
Fragments arranged by size:
1180 954 582 317
BsaI GGTCTCn'nnnn_
Cuts at: 0 835 1018 1118 1364 1909 2533 3033
Size: 835 183 100 246 545 624 500
Fragments arranged by size:
835 624 545 500 246 183 100
BsaAI yAC'GTr
Cuts at: 0 711 1929 3033
Size: 711 1218 1104
Fragments arranged by size:
1218 1104 711
BsaBI GATnn'nnATC
Cuts at: 0 859 3033
Size: 859 2174
BsaHI Gr'CG_yC
Cuts at: 0 1508 1814 2498 2520 2564 2833 2879 3033
Size: 1508 306 684 22 44 269 46 154
Fragments arranged by size:
1508 684 306 269 154 46 44 22
BsaWI w'CCGG_w
Cuts at: 0 1494 2974 3033
Size: 1494 1480 59
Fragments arranged by size:
1494 1480 59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1339 1369 2455 2485 2530 2560 3033
Size: 1339 30 1086 30 45 30 473
Fragments arranged by size:
1339 1086 473 45 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 144 147 972 1246 1321 1324 1329 1363
Size: 144 3 825 274 75 3 5 34
Cuts at: 1363 1366 1435 1438 1468 1990 2289 2356 2952
Size: 3 69 3 30 522 299 67 596
Cuts at: 2952 3033
Size: 81
Fragments arranged by size:
825 596 522 299 274 144 81 75
69 67 34 30 5 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 10 882 943 1000 1817 1884 1984 2009
Size: 10 872 61 57 817 67 100 25
Cuts at: 2009 2303 3033
Size: 294 730
Fragments arranged by size:
872 817 730 294 100 67 61 57
25 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 304 3033
Size: 115 189 2729
Fragments arranged by size:
2729 189 115
BsiEI CG_ry'CG
Cuts at: 0 33 167 452 2285 3033
Size: 33 134 285 1833 748
Fragments arranged by size:
1833 748 285 134 33
BsiHKAI G_wGCw'C
Cuts at: 0 1430 1460 1874 2093 2299 2872 3033
Size: 1430 30 414 219 206 573 161
Fragments arranged by size:
1430 573 414 219 206 161 30
BsmI GAATG_Cn'
Cuts at: 0 513 3033
Size: 513 2520
BsmBI CGTCTCn'nnnn_
Cuts at: 0 292 2338 3033
Size: 292 2046 695
Fragments arranged by size:
2046 695 292
Bsp1286I G_dGCh'C
Cuts at: 0 196 397 460 523 767 799 817 1060
Size: 196 201 63 63 244 32 18 243
Cuts at: 1060 1251 1430 1460 1810 1874 2010 2093 2299
Size: 191 179 30 350 64 136 83 206
Cuts at: 2299 2448 2872 3033
Size: 149 424 161
Fragments arranged by size:
424 350 244 243 206 201 196 191
179 161 149 136 83 64 63 63
32 30 18
BspEI T'CCGG_A
Cuts at: 0 1494 3033
Size: 1494 1539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 662 2901 3033
Size: 662 2239 132
Fragments arranged by size:
2239 662 132
BsrBI CCG'CTC
Cuts at: 0 249 1536 2196 2613 3033
Size: 249 1287 660 417 420
Fragments arranged by size:
1287 660 420 417 249
BsrDI GCAATG_nn'
Cuts at: 0 508 3033
Size: 508 2525
BsrFI r'CCGG_y
Cuts at: 0 592 2036 3033
Size: 592 1444 997
Fragments arranged by size:
1444 997 592
BssSI C'ACGA_G
Cuts at: 0 1258 2092 3033
Size: 1258 834 941
Fragments arranged by size:
1258 941 834
BstAPI GCAn_nnn'nTGC
Cuts at: 0 175 3033
Size: 175 2858
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 325 909 1302 3033
Size: 57 268 584 393 1731
Fragments arranged by size:
1731 584 393 268 57
BstYI r'GATC_y
Cuts at: 0 607 1049 1329 1517 2159 3033
Size: 607 442 280 188 642 874
Fragments arranged by size:
874 642 607 442 280 188
BtgI C'CryG_G
Cuts at: 0 33 56 446 479 914 986 1061 3033
Size: 33 23 390 33 435 72 75 1972
Fragments arranged by size:
1972 435 390 75 72 33 33 23
BtsI GCAGTG_nn'
Cuts at: 0 127 1014 1471 1853 3033
Size: 127 887 457 382 1180
Fragments arranged by size:
1180 887 457 382 127
DraIII CAC_nnn'GTG
Cuts at: 0 480 852 3033
Size: 480 372 2181
Fragments arranged by size:
2181 480 372
EaeI y'GGCC_r
Cuts at: 0 30 101 164 449 524 911 1145 1391
Size: 30 71 63 285 75 387 234 246
Cuts at: 1391 1547 1643 2168 3033
Size: 156 96 525 865
Fragments arranged by size:
865 525 387 285 246 234 156 96
75 71 63 30
EagI C'GGCC_G
Cuts at: 0 30 164 449 3033
Size: 30 134 285 2584
Fragments arranged by size:
2584 285 134 30
EarI CTCTTCn'nnn_
Cuts at: 0 838 1035 3033
Size: 838 197 1998
Fragments arranged by size:
1998 838 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 220 1636 2727 3033
Size: 220 1416 1091 306
Fragments arranged by size:
1416 1091 306 220
EcoICRI GAG'CTC
Cuts at: 0 1428 1458 3033
Size: 1428 30 1575
Fragments arranged by size:
1575 1428 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 40 388 1102 1324 1396 1438 1444
Size: 33 7 348 714 222 72 42 6
Cuts at: 1444 1627 1699 1798 1861 1900 1933 2035 2062
Size: 183 72 99 63 39 33 102 27
Cuts at: 2062 2260 2482 2527 2545 2557 2665 3033
Size: 198 222 45 18 12 108 368
Fragments arranged by size:
714 368 348 222 222 198 183 108
102 99 72 72 63 45 42 39
33 33 27 18 12 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1195 2506 3033
Size: 1195 1311 527
Fragments arranged by size:
1311 1195 527
EcoO109I rG'GnC_Cy
Cuts at: 0 193 651 1483 1602 1782 1944 2289 2373
Size: 193 458 832 119 180 162 345 84
Cuts at: 2373 2444 2679 2748 3033
Size: 71 235 69 285
Fragments arranged by size:
832 458 345 285 235 193 180 162
119 84 71 69
HaeII r_GCGC'y
Cuts at: 0 1817 2070 2523 2808 2836 3033
Size: 1817 253 453 285 28 197
Fragments arranged by size:
1817 453 285 253 197 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 460 492 592 599 613 624 631 645
Size: 460 32 100 7 14 11 7 14
Cuts at: 645 924 956 1339 1371 1433 1465 2530 2562
Size: 279 32 383 32 62 32 1065 32
Cuts at: 2562 2812 2844 3033
Size: 250 32 189
Fragments arranged by size:
1065 460 383 279 250 189 100 62
32 32 32 32 32 32 14 14
11 7 7
KasI G'GCGC_C
Cuts at: 0 1813 2519 2832 3033
Size: 1813 706 313 201
Fragments arranged by size:
1813 706 313 201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 1319 2165 2774 3033
Size: 635 684 846 609 259
Fragments arranged by size:
846 684 635 609 259
MscI TGG'CCA
Cuts at: 0 913 1147 3033
Size: 913 234 1886
Fragments arranged by size:
1886 913 234
MslI CAynn'nnrTG
Cuts at: 0 919 1864 1927 2314 3033
Size: 919 945 63 387 719
Fragments arranged by size:
945 919 719 387 63
MspA1I CmG'CkG
Cuts at: 0 14 172 537 626 1110 1302 1416 1878
Size: 14 158 365 89 484 192 114 462
Cuts at: 1878 1995 2013 2205 2862 3033
Size: 117 18 192 657 171
Fragments arranged by size:
657 484 462 365 192 192 171 158
117 114 89 18 14
NarI GG'CG_CC
Cuts at: 0 1814 2520 2833 3033
Size: 1814 706 313 200
Fragments arranged by size:
1814 706 313 200
NcoI C'CATG_G
Cuts at: 0 56 914 3033
Size: 56 858 2119
Fragments arranged by size:
2119 858 56
NheI G'CTAG_C
Cuts at: 0 106 3033
Size: 106 2927
PasI CC'CwG_GG
Cuts at: 0 2217 2665 3033
Size: 2217 448 368
Fragments arranged by size:
2217 448 368
PflMI CCAn_nnn'nTGG
Cuts at: 0 480 3033
Size: 480 2553
PfoI T'CCnGG_A
Cuts at: 0 2548 3033
Size: 2548 485
PmlI CAC'GTG
Cuts at: 0 1929 3033
Size: 1929 1104
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 3033
Size: 289 32 2712
Fragments arranged by size:
2712 289 32
PpuMI rG'GwC_Cy
Cuts at: 0 651 1483 1782 2679 3033
Size: 651 832 299 897 354
Fragments arranged by size:
897 832 651 354 299
PspOMI G'GGCC_C
Cuts at: 0 192 1806 2006 2444 3033
Size: 192 1614 200 438 589
Fragments arranged by size:
1614 589 438 200 192
PstI C_TGCA'G
Cuts at: 0 1244 1337 1415 1964 1994 2051 2204 2381
Size: 1244 93 78 549 30 57 153 177
Cuts at: 2381 2855 3033
Size: 474 178
Fragments arranged by size:
1244 549 474 178 177 153 93 78
57 30
PvuII CAG'CTG
Cuts at: 0 172 1110 1302 1416 1878 2013 2862 3033
Size: 172 938 192 114 462 135 849 171
Fragments arranged by size:
938 849 462 192 172 171 135 114
SacI G_AGCT'C
Cuts at: 0 1430 1460 3033
Size: 1430 30 1573
Fragments arranged by size:
1573 1430 30
SanDI GG'GwC_CC
Cuts at: 0 1483 3033
Size: 1483 1550
SbfI CC_TGCA'GG
Cuts at: 0 2381 3033
Size: 2381 652
SexAI A'CCwGG_T
Cuts at: 0 799 3033
Size: 799 2234
SfcI C'TryA_G
Cuts at: 0 1240 1333 1411 1960 1990 2047 2200 2377
Size: 1240 93 78 549 30 57 153 177
Cuts at: 2377 2851 3033
Size: 474 182
Fragments arranged by size:
1240 549 474 182 177 153 93 78
57 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1609 3033
Size: 1609 1424
SfoI GGC'GCC
Cuts at: 0 1815 2521 2834 3033
Size: 1815 706 313 199
Fragments arranged by size:
1815 706 313 199
SmlI C'TyrA_G
Cuts at: 0 1195 2149 2731 3033
Size: 1195 954 582 302
Fragments arranged by size:
1195 954 582 302
StuI AGG'CCT
Cuts at: 0 1612 3033
Size: 1612 1421
StyI C'CwwG_G
Cuts at: 0 56 113 197 731 768 887 914 1148
Size: 56 57 84 534 37 119 27 234
Cuts at: 1148 1364 3033
Size: 216 1669
Fragments arranged by size:
1669 534 234 216 119 84 57 56
37 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 246 834 907 1517 2326 2446 3033
Size: 246 588 73 610 809 120 587
Fragments arranged by size:
809 610 588 587 246 120 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1756 1788 2455 2487 3033
Size: 1756 32 667 32 546
Fragments arranged by size:
1756 667 546 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2544 3033
Size: 2544 489
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1371 1570 1710 1908 3033
Size: 1371 199 140 198 1125
Fragments arranged by size:
1371 1125 199 198 140
Enzymes that do cut:
AarI AcuI AhdI AlfI AloI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI
BstYI BtgI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI
MscI MslI MspA1I NarI NcoI NheI PasI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI
SanDI SbfI SexAI SfcI SfiI SfoI SmlI StuI
StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI ApoI AscI AseI AsiSI AvrII BaeI BcgI
BciVI BclI BfrBI BsiWI BspHI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NotI
NruI NsiI NspI PacI PciI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SapI ScaI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XhoI XmaI XmnI ZraI