(Circular) (Six-base) MAPSORT of: pFN21AA0261.seq Check: 1114 from: 1 to: 8530 pFN21AA0261 8530 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2139 2139 Size: 8530 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6340 278 Size: 53 83 186 936 4804 2468 Fragments arranged by size: 4804 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5873 1937 Size: 3936 4594 Acc65I G'GTAC_C Cuts at: 5851 5851 Size: 8530 AclI AA'CG_TT Cuts at: 1563 6657 7030 1563 Size: 5094 373 3063 Fragments arranged by size: 5094 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2574 2739 5141 5570 6593 7977 8245 1632 Size: 942 165 2402 429 1023 1384 268 1917 Fragments arranged by size: 2402 1917 1384 1023 942 429 268 165 AfeI AGC'GCT Cuts at: 5142 5142 Size: 8530 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8333 AflIII A'CryG_T Cuts at: 1203 4277 7429 8406 1203 Size: 3074 3152 977 1327 Fragments arranged by size: 3152 3074 1327 977 AgeI A'CCGG_T Cuts at: 7361 7361 Size: 8530 AhdI GACnn_n'nnGTC Cuts at: 3763 7259 3763 Size: 3496 5034 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8530 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4147 1184 Size: 2963 5567 AlwNI CAG_nnn'CTG Cuts at: 4405 6273 7845 4405 Size: 1868 1572 5090 Fragments arranged by size: 5090 1868 1572 ApaI G_GGCC'C Cuts at: 2018 2018 Size: 8530 ApaLI G'TGCA_C Cuts at: 6587 7743 6587 Size: 1156 7374 ApoI r'AATT_y Cuts at: 1493 2315 2430 2521 2770 3187 3539 4199 5741 Size: 822 115 91 249 417 352 660 1542 Cuts at: 5741 5839 6065 6217 8326 1493 Size: 98 226 152 2109 1697 Fragments arranged by size: 2109 1697 1542 822 660 417 352 249 226 152 115 98 91 AseI AT'TA_AT Cuts at: 160 1032 2747 7084 160 Size: 872 1715 4337 1606 Fragments arranged by size: 4337 1715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8530 AvaI C'yCGr_G Cuts at: 1870 1943 5855 1870 Size: 73 3912 4545 Fragments arranged by size: 4545 3912 73 BamHI G'GATC_C Cuts at: 5860 6227 5860 Size: 367 8163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2058 3209 5851 7306 618 Size: 325 217 627 271 1151 2642 1455 1842 Fragments arranged by size: 2642 1842 1455 1151 627 325 271 217 BanII G_rGCy'C Cuts at: 1391 2018 5849 1391 Size: 627 3831 4072 Fragments arranged by size: 4072 3831 627 BbeI G_GCGC'C Cuts at: 2062 2062 Size: 8530 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2640 3012 4002 4149 928 Size: 971 741 372 990 147 5309 Fragments arranged by size: 5309 990 971 741 372 147 BbvCI CC'TCA_GC Cuts at: 4962 4962 Size: 8530 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6721 6755 1903 Size: 34 4784 34 3678 Fragments arranged by size: 4784 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3376 6422 7632 3376 Size: 3046 1210 4274 Fragments arranged by size: 4274 3046 1210 BclI T'GATC_A Cuts at: 1548 5430 1548 Size: 3882 4648 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7141 136 Size: 107 122 71 631 605 5469 1525 Fragments arranged by size: 5469 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4483 8525 4483 Size: 4042 4488 BlpI GC'TnA_GC Cuts at: 4978 5947 4978 Size: 969 7561 Bme1580I G_kGCm'C Cuts at: 1790 2018 6591 7747 1790 Size: 228 4573 1156 2573 Fragments arranged by size: 4573 2573 1156 228 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8530 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2893 4317 4641 Size: 416 53 83 399 24 1476 1424 324 Cuts at: 4641 6261 7219 442 Size: 1620 958 1753 Fragments arranged by size: 1753 1620 1476 1424 958 416 399 324 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4479 5408 1056 Size: 3423 929 4178 Fragments arranged by size: 4178 3423 929 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2013 2487 7190 1239 Size: 774 474 4703 2579 Fragments arranged by size: 4703 2579 774 474 Bpu10I CC'TnA_GC Cuts at: 2351 3223 4962 5212 2351 Size: 872 1739 250 5669 Fragments arranged by size: 5669 1739 872 250 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5402 6655 7520 7818 5402 Size: 1253 865 298 6114 Fragments arranged by size: 6114 1253 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2363 5132 7193 882 Size: 617 864 2769 2061 2219 Fragments arranged by size: 2769 2219 2061 864 617 BsaAI yAC'GTr Cuts at: 493 1204 4174 6321 493 Size: 711 2970 2147 2702 Fragments arranged by size: 2970 2702 2147 711 BsaBI GATnn'nnATC Cuts at: 3030 8524 3030 Size: 5494 3036 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2059 6337 6719 7354 Size: 53 83 186 936 526 4278 382 635 Cuts at: 7354 275 Size: 1451 Fragments arranged by size: 4278 1451 936 635 526 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6963 7361 7635 7782 8193 6963 Size: 398 274 147 411 7300 Fragments arranged by size: 7300 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8305 Fragments arranged by size: 8305 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2250 5564 5567 1186 Size: 1064 3314 3 4149 Fragments arranged by size: 4149 3314 1064 3 BseYI C'CCAG_C Cuts at: 4389 5206 5400 7733 8229 4389 Size: 817 194 2333 496 4690 Fragments arranged by size: 4690 2333 817 496 194 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4041 4107 5390 1163 Size: 573 2305 66 1283 4303 Fragments arranged by size: 4303 2305 1283 573 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5980 6741 7769 1540 Size: 275 186 3979 761 1028 2301 Fragments arranged by size: 3979 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2699 4262 4348 5248 5288 6591 6676 7747 2699 Size: 1563 86 900 40 1303 85 1071 3482 Fragments arranged by size: 3482 1563 1303 1071 900 86 85 40 BsmI GAATG_Cn' Cuts at: 4338 6041 6134 4338 Size: 1703 93 6734 Fragments arranged by size: 6734 1703 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3455 3455 Size: 8530 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2699 4262 4348 5248 5288 5849 Size: 399 228 681 1563 86 900 40 561 Cuts at: 5849 6591 6676 7747 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1563 1071 900 742 681 561 399 228 86 85 40 BspEI T'CCGG_A Cuts at: 8193 8193 Size: 8530 BspHI T'CATG_A Cuts at: 2776 3559 6419 8149 2776 Size: 783 2860 1730 3157 Fragments arranged by size: 3157 2860 1730 783 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 5885 844 Size: 1295 3746 3489 Fragments arranged by size: 3746 3489 1295 BsrBI CCG'CTC Cuts at: 1394 1823 2064 2188 5392 6417 1394 Size: 429 241 124 3204 1025 3507 Fragments arranged by size: 3507 3204 1025 429 241 124 BsrDI GCAATG_nn' Cuts at: 65 1248 3748 4368 5824 7025 7199 65 Size: 1183 2500 620 1456 1201 174 1396 Fragments arranged by size: 2500 1456 1396 1201 1183 620 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2046 2134 5594 7174 7361 1700 Size: 110 236 88 3460 1580 187 2869 Fragments arranged by size: 3460 2869 1580 236 187 110 88 BsrGI T'GTAC_A Cuts at: 96 3109 4415 4594 96 Size: 3013 1306 179 4032 Fragments arranged by size: 4032 3013 1306 179 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8530 BssSI C'ACGA_G Cuts at: 6590 7602 6590 Size: 1012 7518 BstAPI GCAn_nnn'nTGC Cuts at: 794 3470 794 Size: 2676 5854 BstBI TT'CG_AA Cuts at: 4330 7334 4330 Size: 3004 5526 BstEII G'GTnAC_C Cuts at: 1184 3903 1184 Size: 2719 5811 BstXI CCAn_nnnn'nTGG Cuts at: 2380 2380 Size: 8530 BstYI r'GATC_y Cuts at: 1972 2470 2948 4462 4483 5860 6227 6612 6629 Size: 498 478 1514 21 1377 367 385 17 Cuts at: 6629 8081 8167 8179 8525 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1514 1452 1377 498 478 385 367 346 86 21 17 12 Bsu36I CC'TnA_GG Cuts at: 5178 5178 Size: 8530 BtgI C'CryG_G Cuts at: 513 1065 2004 2172 2208 513 Size: 552 939 168 36 6835 Fragments arranged by size: 6835 939 552 168 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7791 Fragments arranged by size: 7791 683 56 BtsI GCAGTG_nn' Cuts at: 3411 5040 6046 6840 6860 3411 Size: 1629 1006 794 20 5081 Fragments arranged by size: 5081 1629 1006 794 20 ClaI AT'CG_AT Cuts at: 1554 3070 1554 Size: 1516 7014 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8495 DraI TTT'AAA Cuts at: 3064 4372 4693 5834 3064 Size: 1308 321 1141 5760 Fragments arranged by size: 5760 1308 1141 321 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7537 809 Size: 507 6221 1802 Fragments arranged by size: 6221 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 5785 5977 Size: 54 1425 50 275 18 381 3574 192 Cuts at: 5977 6866 8495 8 Size: 889 1629 43 Fragments arranged by size: 3574 1629 1425 889 381 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5977 1537 Size: 275 4165 4090 Fragments arranged by size: 4165 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2393 2574 4776 5526 6460 1353 Size: 1040 181 2202 750 934 3423 Fragments arranged by size: 3423 2202 1040 934 750 181 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2065 2212 7117 7491 7637 387 Size: 1064 138 476 147 4905 374 146 1280 Fragments arranged by size: 4905 1280 1064 476 374 147 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2013 2487 2574 2739 5141 5570 6593 Size: 393 381 474 87 165 2402 429 1023 Cuts at: 6593 7190 7977 8245 1239 Size: 597 787 268 1524 Fragments arranged by size: 2402 1524 1023 787 597 474 429 393 381 268 165 87 EcoNI CCTnn'n_nnAGG Cuts at: 3263 5177 3263 Size: 1914 6616 EcoO109I rG'GnC_Cy Cuts at: 2014 2015 2014 Size: 1 8529 EcoRI G'AATT_C Cuts at: 5839 6217 5839 Size: 378 8152 EcoRV GAT'ATC Cuts at: 2422 2995 2422 Size: 573 7957 FspI TGC'GCA Cuts at: 7036 7036 Size: 8530 HaeII r_GCGC'y Cuts at: 1678 2062 5144 7677 1678 Size: 384 3082 2533 2531 Fragments arranged by size: 3082 2533 2531 384 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2483 2515 2984 3016 3749 Size: 32 135 32 911 32 469 32 733 Cuts at: 3749 3781 4432 4464 4508 4540 7171 7203 7245 Size: 32 651 32 44 32 2631 32 42 Cuts at: 7245 7277 1373 Size: 32 2626 Fragments arranged by size: 2631 2626 911 733 651 469 135 44 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5269 5508 5874 6120 669 Size: 988 281 3331 239 366 246 3079 Fragments arranged by size: 3331 3079 988 366 281 246 239 HindIII A'AGCT_T Cuts at: 748 748 Size: 8530 HpaI GTT'AAC Cuts at: 6120 6120 Size: 8530 KasI G'GCGC_C Cuts at: 2058 2058 Size: 8530 KpnI G_GTAC'C Cuts at: 5855 5855 Size: 8530 MfeI C'AATT_G Cuts at: 6129 6129 Size: 8530 MluI A'CGCG_T Cuts at: 8406 8406 Size: 8530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2358 2556 3295 5755 7644 7828 1305 Size: 1053 198 739 2460 1889 184 2007 Fragments arranged by size: 2460 2007 1889 1053 739 198 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2213 5787 10 Size: 54 1425 343 381 3574 2753 Fragments arranged by size: 3574 2753 1425 381 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2378 2814 4129 5007 5063 6488 6847 Size: 648 1212 436 1315 878 56 1425 359 Cuts at: 6847 7006 518 Size: 159 2042 Fragments arranged by size: 2042 1425 1315 1212 878 648 436 359 159 56 MspA1I CmG'CkG Cuts at: 1606 2174 4421 5946 6623 7771 8016 1606 Size: 568 2247 1525 677 1148 245 2120 Fragments arranged by size: 2247 2120 1525 1148 677 568 245 NaeI GCC'GGC Cuts at: 2136 5596 2136 Size: 3460 5070 NarI GG'CG_CC Cuts at: 2059 2059 Size: 8530 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7039 Fragments arranged by size: 7039 939 552 NdeI CA'TA_TG Cuts at: 387 4973 387 Size: 4586 3944 NgoMIV G'CCGG_C Cuts at: 2134 5594 2134 Size: 3460 5070 NheI G'CTAG_C Cuts at: 1052 4475 5404 1052 Size: 3423 929 4178 Fragments arranged by size: 4178 3423 929 NotI GC'GGCC_GC Cuts at: 5977 5977 Size: 8530 NspI r_CATG'y Cuts at: 1132 1229 5594 5888 7433 1132 Size: 97 4365 294 1545 2229 Fragments arranged by size: 4365 2229 1545 294 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8530 PciI A'CATG_T Cuts at: 7429 7429 Size: 8530 PflMI CCAn_nnn'nTGG Cuts at: 1108 3936 1108 Size: 2828 5702 PmeI GTTT'AAAC Cuts at: 5834 5834 Size: 8530 PmlI CAC'GTG Cuts at: 4174 4174 Size: 8530 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5556 5588 6599 6631 8112 8144 5556 Size: 32 1011 32 1481 32 5942 Fragments arranged by size: 5942 1481 1011 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8530 PsiI TTA'TAA Cuts at: 6100 6100 Size: 8530 PspOMI G'GGCC_C Cuts at: 2014 2014 Size: 8530 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3102 3134 3102 Size: 32 8498 PstI C_TGCA'G Cuts at: 830 3430 5882 830 Size: 2600 2452 3478 Fragments arranged by size: 3478 2600 2452 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8530 PvuII CAG'CTG Cuts at: 4421 4421 Size: 8530 SacII CC_GC'GG Cuts at: 2175 2175 Size: 8530 SalI G'TCGA_C Cuts at: 1936 5872 1936 Size: 3936 4594 SbfI CC_TGCA'GG Cuts at: 5882 5882 Size: 8530 ScaI AGT'ACT Cuts at: 2464 2595 2830 4238 6778 8490 2464 Size: 131 235 1408 2540 1712 2504 Fragments arranged by size: 2540 2504 1712 1408 235 131 SfcI C'TryA_G Cuts at: 826 1045 2853 3136 3148 3426 5305 5878 7013 Size: 219 1808 283 12 278 1879 573 1135 Cuts at: 7013 7694 7885 826 Size: 681 191 1471 Fragments arranged by size: 1879 1808 1471 1135 681 573 283 278 219 191 12 SfoI GGC'GCC Cuts at: 2060 2060 Size: 8530 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8530 SgrAI Cr'CCGG_yG Cuts at: 1700 2046 1700 Size: 346 8184 SmaI CCC'GGG Cuts at: 1872 5857 1872 Size: 3985 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5381 6634 7535 7797 820 Size: 197 926 3438 1253 901 262 1553 Fragments arranged by size: 3438 1553 1253 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6321 493 Size: 5828 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8530 SphI G_CATG'C Cuts at: 1132 5594 5888 1132 Size: 4462 294 3774 Fragments arranged by size: 4462 3774 294 SspI AAT'ATT Cuts at: 5 52 5816 6454 5 Size: 47 5764 638 2081 Fragments arranged by size: 5764 2081 638 47 StuI AGG'CCT Cuts at: 1517 3221 1517 Size: 1704 6826 StyI C'CwwG_G Cuts at: 513 1065 2004 3175 4377 5969 513 Size: 552 939 1171 1202 1592 3074 Fragments arranged by size: 3074 1592 1202 1171 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3922 4703 5491 5637 6557 6574 6727 Size: 1255 2342 781 788 146 920 17 153 Cuts at: 6727 6912 7357 325 Size: 185 445 1498 Fragments arranged by size: 2342 1498 1255 920 788 781 445 185 153 146 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2462 2593 2828 Size: 274 80 33 51 1443 485 131 235 Cuts at: 2828 3109 4236 4415 4594 4950 6776 8488 96 Size: 281 1127 179 179 356 1826 1712 138 Fragments arranged by size: 1826 1712 1443 1127 485 356 281 274 235 179 179 138 131 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5267 5299 8490 8522 5267 Size: 32 3191 32 5275 Fragments arranged by size: 5275 3191 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2639 1316 Size: 1323 7207 XbaI T'CTAG_A Cuts at: 4615 5801 5866 4615 Size: 1186 65 7279 Fragments arranged by size: 7279 1186 65 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8530 XmaI C'CCGG_G Cuts at: 1870 5855 1870 Size: 3985 4545 XmnI GAAnn'nnTTC Cuts at: 4181 4941 6659 4181 Size: 760 1718 6052 Fragments arranged by size: 6052 1718 760 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6338 276 Size: 53 83 186 936 4804 2468 Fragments arranged by size: 4804 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI