(Circular) (Six-base) MAPSORT of: pFN21AA0236.seq Check: 7375 from: 1 to: 9766 pFN21AA0236 9766 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2556 4375 4665 4795 7005 2556 Size: 1819 290 130 2210 5317 Fragments arranged by size: 5317 2210 1819 290 130 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7576 278 Size: 53 83 186 936 6040 2468 Fragments arranged by size: 6040 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7109 1937 Size: 5172 4594 Acc65I G'GTAC_C Cuts at: 4745 5640 5717 7087 4745 Size: 895 77 1370 7424 Fragments arranged by size: 7424 1370 895 77 AclI AA'CG_TT Cuts at: 1563 7893 8266 1563 Size: 6330 373 3063 Fragments arranged by size: 6330 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2223 2379 2945 3736 4281 4682 5072 5163 Size: 591 156 566 791 545 401 390 91 Cuts at: 5163 6207 6623 7829 9213 9481 1632 Size: 1044 416 1206 1384 268 1917 Fragments arranged by size: 1917 1384 1206 1044 791 591 566 545 416 401 390 268 156 91 AfeI AGC'GCT Cuts at: 2513 2513 Size: 9766 AflII C'TTAA_G Cuts at: 820 1017 5452 5906 820 Size: 197 4435 454 4680 Fragments arranged by size: 4680 4435 454 197 AflIII A'CryG_T Cuts at: 1203 3375 5327 8665 9642 1203 Size: 2172 1952 3338 977 1327 Fragments arranged by size: 3338 2172 1952 1327 977 AgeI A'CCGG_T Cuts at: 5643 5742 8597 5643 Size: 99 2855 6812 Fragments arranged by size: 6812 2855 99 AhdI GACnn_n'nnGTC Cuts at: 8495 8495 Size: 9766 AleI CACnn'nnGTG Cuts at: 1166 2682 3294 1166 Size: 1516 612 7638 Fragments arranged by size: 7638 1516 612 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4673 5499 1184 Size: 3489 826 5451 Fragments arranged by size: 5451 3489 826 AlwNI CAG_nnn'CTG Cuts at: 2201 2246 2446 3970 4292 4883 5421 7509 9081 Size: 45 200 1524 322 591 538 2088 1572 Cuts at: 9081 2201 Size: 2886 Fragments arranged by size: 2886 2088 1572 1524 591 538 322 200 45 ApaI G_GGCC'C Cuts at: 3866 5505 5984 3866 Size: 1639 479 7648 Fragments arranged by size: 7648 1639 479 ApaLI G'TGCA_C Cuts at: 3379 3425 6364 6662 6992 7823 8979 3379 Size: 46 2939 298 330 831 1156 4166 Fragments arranged by size: 4166 2939 1156 831 330 298 46 ApoI r'AATT_y Cuts at: 1493 2786 7075 7301 7453 9562 1493 Size: 1293 4289 226 152 2109 1697 Fragments arranged by size: 4289 2109 1697 1293 226 152 AseI AT'TA_AT Cuts at: 160 1032 8320 160 Size: 872 7288 1606 Fragments arranged by size: 7288 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9766 AvaI C'yCGr_G Cuts at: 1870 1943 2142 4610 5390 5933 6171 6620 7091 Size: 73 199 2468 780 543 238 449 471 Cuts at: 7091 1870 Size: 4545 Fragments arranged by size: 4545 2468 780 543 471 449 238 199 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6509 6542 6509 Size: 33 9733 BamHI G'GATC_C Cuts at: 6504 7018 7096 7463 6504 Size: 514 78 367 8807 Fragments arranged by size: 8807 514 367 78 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2468 4578 4745 5475 5640 Size: 325 217 627 681 2110 167 730 165 Cuts at: 5640 5717 5849 6930 7087 8542 618 Size: 77 132 1081 157 1455 1842 Fragments arranged by size: 2110 1842 1455 1081 730 681 627 325 217 167 165 157 132 77 BanII G_rGCy'C Cuts at: 1391 2132 2440 3866 3891 4299 4599 5311 5359 Size: 741 308 1426 25 408 300 712 48 Cuts at: 5359 5505 5984 7085 1391 Size: 146 479 1101 4072 Fragments arranged by size: 4072 1426 1101 741 712 479 408 308 300 146 48 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4009 928 Size: 971 2110 6685 Fragments arranged by size: 6685 2110 971 BbvCI CC'TCA_GC Cuts at: 2795 4646 2795 Size: 1851 7915 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7957 7991 1903 Size: 34 6020 34 3678 Fragments arranged by size: 6020 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5272 7658 8868 5272 Size: 2386 1210 6170 Fragments arranged by size: 6170 2386 1210 BclI T'GATC_A Cuts at: 1548 3496 1548 Size: 1948 7818 BfrBI ATG'CAT Cuts at: 2728 3187 2728 Size: 459 9307 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3005 3105 5847 Size: 107 122 71 631 605 1333 100 2742 Cuts at: 5847 8377 136 Size: 2530 1525 Fragments arranged by size: 2742 2530 1525 1333 631 605 122 107 100 71 BglII A'GATC_T Cuts at: 9761 9761 Size: 9766 BlpI GC'TnA_GC Cuts at: 2439 3503 3683 7183 2439 Size: 1064 180 3500 5022 Fragments arranged by size: 5022 3500 1064 180 Bme1580I G_kGCm'C Cuts at: 1790 2069 2585 3383 3429 3733 3866 4610 4673 Size: 279 516 798 46 304 133 744 63 Cuts at: 4673 5505 5612 5984 6017 6350 6368 6536 6666 Size: 832 107 372 33 333 18 168 130 Cuts at: 6666 6996 7827 8983 1790 Size: 330 831 1156 2573 Fragments arranged by size: 2573 1156 832 831 798 744 516 372 333 330 304 279 168 133 130 107 63 46 33 18 BmgBI CAC'GTC Cuts at: 1318 3325 5162 1318 Size: 2007 1837 5922 Fragments arranged by size: 5922 2007 1837 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3149 4348 4584 Size: 416 53 83 399 24 1732 1199 236 Cuts at: 4584 4886 4946 5006 5866 5878 6438 7497 8455 Size: 302 60 60 860 12 560 1059 958 Cuts at: 8455 442 Size: 1753 Fragments arranged by size: 1753 1732 1199 1059 958 860 560 416 399 302 236 83 60 60 53 24 12 BmtI G_CTAG'C Cuts at: 1056 4626 1056 Size: 3570 6196 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5281 5281 Size: 9766 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2283 2292 2561 3825 4136 4552 5421 7034 Size: 1044 9 269 1264 311 416 869 1613 Cuts at: 7034 8426 1239 Size: 1392 2579 Fragments arranged by size: 2579 1613 1392 1264 1044 869 416 311 269 9 Bpu10I CC'TnA_GC Cuts at: 2070 2795 4646 2070 Size: 725 1851 7190 Fragments arranged by size: 7190 1851 725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2606 2804 4160 4400 5564 6086 6473 7891 8756 Size: 198 1356 240 1164 522 387 1418 865 Cuts at: 8756 9054 2606 Size: 298 3318 Fragments arranged by size: 3318 1418 1356 1164 865 522 387 298 240 198 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2270 2467 2924 3801 5963 6665 8429 Size: 617 771 197 457 877 2162 702 1764 Cuts at: 8429 882 Size: 2219 Fragments arranged by size: 2219 2162 1764 877 771 702 617 457 197 BsaAI yAC'GTr Cuts at: 493 1204 5137 6922 7557 493 Size: 711 3933 1785 635 2702 Fragments arranged by size: 3933 2702 1785 711 635 BsaBI GATnn'nnATC Cuts at: 9760 9760 Size: 9766 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7573 7955 8590 275 Size: 53 83 186 936 6040 382 635 1451 Fragments arranged by size: 6040 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5643 5742 6120 8199 8597 8871 9018 9429 5643 Size: 99 378 2079 398 274 147 411 5980 Fragments arranged by size: 5980 2079 411 398 378 274 147 99 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2269 2299 3458 3488 4294 Size: 30 165 30 866 30 1159 30 806 Cuts at: 4294 4324 4722 4752 5075 5105 1178 Size: 30 398 30 323 30 5839 Fragments arranged by size: 5839 1159 866 806 398 323 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 4001 4085 4157 4337 4963 5023 5110 Size: 1192 1623 84 72 180 626 60 87 Cuts at: 5110 5408 6230 1186 Size: 298 822 4722 Fragments arranged by size: 4722 1623 1192 822 626 298 180 87 84 72 60 BseYI C'CCAG_C Cuts at: 2281 2570 2644 3603 3953 4052 4057 5196 5491 Size: 289 74 959 350 99 5 1139 295 Cuts at: 5491 5854 6017 6813 7022 8969 9465 2281 Size: 363 163 796 209 1947 496 2582 Fragments arranged by size: 2582 1947 1139 959 796 496 363 350 295 289 209 163 99 74 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2136 3109 4066 4496 4547 5393 5925 Size: 573 400 973 957 430 51 846 532 Cuts at: 5925 5972 6619 7028 1163 Size: 47 647 409 3901 Fragments arranged by size: 3901 973 957 846 647 573 532 430 409 400 51 47 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2902 6856 7216 7977 9005 1540 Size: 275 186 901 3954 360 761 1028 2301 Fragments arranged by size: 3954 2301 1028 901 761 360 275 186 BsiHKAI G_wGCw'C Cuts at: 2269 2547 2817 3252 3383 3429 3476 4740 6120 Size: 278 270 435 131 46 47 1264 1380 Cuts at: 6120 6368 6666 6996 7827 7912 8983 2269 Size: 248 298 330 831 85 1071 3052 Fragments arranged by size: 3052 1380 1264 1071 831 435 330 298 278 270 248 131 85 47 46 BsiWI C'GTAC_G Cuts at: 6922 6922 Size: 9766 BsmI GAATG_Cn' Cuts at: 2704 7277 7370 2704 Size: 4573 93 5100 Fragments arranged by size: 5100 4573 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3315 6359 6491 3315 Size: 3044 132 6590 Fragments arranged by size: 6590 3044 132 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2132 2269 2440 2547 2585 2817 Size: 399 279 63 137 171 107 38 232 Cuts at: 2817 3252 3383 3429 3476 3733 3866 3891 4299 Size: 435 131 46 47 257 133 25 408 Cuts at: 4299 4599 4610 4673 4740 5311 5359 5505 5612 Size: 300 11 63 67 571 48 146 107 Cuts at: 5612 5984 6017 6120 6350 6368 6536 6666 6996 Size: 372 33 103 230 18 168 130 330 Cuts at: 6996 7085 7827 7912 8983 1391 Size: 89 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 571 435 408 399 372 330 300 279 257 232 230 171 168 146 137 133 131 130 107 107 103 89 85 67 63 63 48 47 46 38 33 25 18 11 BspEI T'CCGG_A Cuts at: 9429 9429 Size: 9766 BspHI T'CATG_A Cuts at: 3193 7655 9385 3193 Size: 4462 1730 3574 Fragments arranged by size: 4462 3574 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2318 2556 4375 4665 4795 7005 7121 844 Size: 1474 238 1819 290 130 2210 116 3489 Fragments arranged by size: 3489 2210 1819 1474 290 238 130 116 BsrBI CCG'CTC Cuts at: 1394 1823 6178 7043 7653 1394 Size: 429 4355 865 610 3507 Fragments arranged by size: 4355 3507 865 610 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5227 6796 8261 8435 65 Size: 1183 3979 1569 1465 174 1396 Fragments arranged by size: 3979 1569 1465 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5571 5643 5742 8410 8597 1700 Size: 110 3761 72 99 2668 187 2869 Fragments arranged by size: 3761 2869 2668 187 110 99 72 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9766 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9766 BssSI C'ACGA_G Cuts at: 5585 7826 8838 5585 Size: 2241 1012 6513 Fragments arranged by size: 6513 2241 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3132 3471 3679 4735 5094 5421 5691 5700 Size: 2338 339 208 1056 359 327 270 9 Cuts at: 5700 5920 5948 6795 7006 794 Size: 220 28 847 211 3554 Fragments arranged by size: 3554 2338 1056 847 359 339 327 270 220 211 208 28 9 BstBI TT'CG_AA Cuts at: 8570 8570 Size: 9766 BstEII G'GTnAC_C Cuts at: 1184 4034 1184 Size: 2850 6916 BstXI CCAn_nnnn'nTGG Cuts at: 5714 5714 Size: 9766 BstYI r'GATC_y Cuts at: 1972 3201 5373 6504 7018 7096 7463 7848 7865 Size: 1229 2172 1131 514 78 367 385 17 Cuts at: 7865 9317 9403 9415 9761 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2172 1977 1452 1229 1131 514 385 367 346 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 3452 3915 4424 7059 3452 Size: 463 509 2635 6159 Fragments arranged by size: 6159 2635 509 463 BtgI C'CryG_G Cuts at: 513 1065 2104 2935 3000 3790 4027 4980 5497 Size: 552 1039 831 65 790 237 953 517 Cuts at: 5497 6059 6231 513 Size: 562 172 4048 Fragments arranged by size: 4048 1039 953 831 790 562 552 517 237 172 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9027 Fragments arranged by size: 9027 683 56 BtsI GCAGTG_nn' Cuts at: 2662 3034 3115 3231 4021 4522 4939 5083 5410 Size: 372 81 116 790 501 417 144 327 Cuts at: 5410 5430 5445 5481 6277 6653 6716 7282 8076 Size: 20 15 36 796 376 63 566 794 Cuts at: 8076 8096 2662 Size: 20 4332 Fragments arranged by size: 4332 796 794 790 566 501 417 376 372 327 144 116 81 63 36 20 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9766 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9731 DraI TTT'AAA Cuts at: 7070 7070 Size: 9766 DraIII CAC_nnn'GTG Cuts at: 3405 5789 6331 6659 3405 Size: 2384 542 328 6512 Fragments arranged by size: 6512 2384 542 328 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8773 809 Size: 507 7457 1802 Fragments arranged by size: 7457 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2718 5680 6262 Size: 54 1425 50 275 18 888 2962 582 Cuts at: 6262 7213 8102 9731 8 Size: 951 889 1629 43 Fragments arranged by size: 2962 1629 1425 951 889 888 582 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7213 1537 Size: 275 5401 4090 Fragments arranged by size: 5401 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 2355 2376 2381 2486 3108 4857 7696 Size: 846 156 21 5 105 622 1749 2839 Cuts at: 7696 1353 Size: 3423 Fragments arranged by size: 3423 2839 1749 846 622 156 105 21 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2780 8353 8727 8873 387 Size: 1064 138 1191 5573 374 146 1280 Fragments arranged by size: 5573 1280 1191 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2283 2292 2379 2561 2945 3736 Size: 393 591 60 9 87 182 384 791 Cuts at: 3736 3825 4136 4281 4552 4682 5072 5163 5421 Size: 89 311 145 271 130 390 91 258 Cuts at: 5421 6207 6623 7034 7829 8426 9213 9481 1239 Size: 786 416 411 795 597 787 268 1524 Fragments arranged by size: 1524 795 791 787 786 597 591 416 411 393 390 384 311 271 268 258 182 145 130 91 89 87 60 9 EcoNI CCTnn'n_nnAGG Cuts at: 2798 4142 4902 4962 5022 2798 Size: 1344 760 60 60 7542 Fragments arranged by size: 7542 1344 760 60 60 EcoO109I rG'GnC_Cy Cuts at: 2117 2146 2803 3448 3862 3967 4099 4176 4420 Size: 29 657 645 414 105 132 77 244 Cuts at: 4420 4552 4927 4987 5047 5184 5502 5980 5981 Size: 132 375 60 60 137 318 478 1 Cuts at: 5981 6075 2117 Size: 94 5808 Fragments arranged by size: 5808 657 645 478 414 375 318 244 137 132 132 105 94 77 60 60 29 1 EcoRI G'AATT_C Cuts at: 2786 7075 7453 2786 Size: 4289 378 5099 Fragments arranged by size: 5099 4289 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2533 4886 4960 5080 6751 2533 Size: 2353 74 120 1671 5548 Fragments arranged by size: 5548 2353 1671 120 74 FspI TGC'GCA Cuts at: 6746 8272 6746 Size: 1526 8240 HaeII r_GCGC'y Cuts at: 1678 2515 8913 1678 Size: 837 6398 2531 Fragments arranged by size: 6398 2531 837 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2119 2151 2269 2301 2447 Size: 32 135 32 547 32 118 32 146 Cuts at: 2447 2479 3175 3207 4103 4135 4391 4423 6387 Size: 32 696 32 896 32 256 32 1964 Cuts at: 6387 6419 6664 6696 8407 8439 8481 8513 1373 Size: 32 245 32 1711 32 42 32 2626 Fragments arranged by size: 2626 1964 1711 896 696 547 256 245 146 135 118 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3994 5833 6565 7110 7356 669 Size: 988 281 2056 1839 732 545 246 3079 Fragments arranged by size: 3079 2056 1839 988 732 545 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9766 HpaI GTT'AAC Cuts at: 7356 7356 Size: 9766 KpnI G_GTAC'C Cuts at: 4749 5644 5721 7091 4749 Size: 895 77 1370 7424 Fragments arranged by size: 7424 1370 895 77 MfeI C'AATT_G Cuts at: 7365 7365 Size: 9766 MluI A'CGCG_T Cuts at: 9642 9642 Size: 9766 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2766 3280 3384 8880 9064 1305 Size: 1461 514 104 5496 184 2007 Fragments arranged by size: 5496 2007 1461 514 184 104 MscI TGG'CCA Cuts at: 10 64 1489 1832 2720 6264 10 Size: 54 1425 343 888 3544 3512 Fragments arranged by size: 3544 3512 1425 888 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2682 3192 3276 3294 3795 4032 6613 Size: 648 1516 510 84 18 501 237 2581 Cuts at: 6613 6999 7724 8083 8242 518 Size: 386 725 359 159 2042 Fragments arranged by size: 2581 2042 1516 725 648 510 501 386 359 237 159 84 18 MspA1I CmG'CkG Cuts at: 1606 2357 2443 3502 3901 4080 4515 5838 6000 Size: 751 86 1059 399 179 435 1323 162 Cuts at: 6000 6147 6313 6571 6813 7012 7182 7859 9007 Size: 147 166 258 242 199 170 677 1148 Cuts at: 9007 9252 1606 Size: 245 2120 Fragments arranged by size: 2120 1323 1148 1059 751 677 435 399 258 245 242 199 179 170 166 162 147 86 NcoI C'CATG_G Cuts at: 513 1065 2935 3000 3790 4027 513 Size: 552 1870 65 790 237 6252 Fragments arranged by size: 6252 1870 790 552 237 65 NdeI CA'TA_TG Cuts at: 387 2493 387 Size: 2106 7660 NheI G'CTAG_C Cuts at: 1052 4622 1052 Size: 3570 6196 NotI GC'GGCC_GC Cuts at: 7213 7213 Size: 9766 NsiI A_TGCA'T Cuts at: 2730 3189 2730 Size: 459 9307 NspI r_CATG'y Cuts at: 1132 1229 2728 3191 3265 3318 3349 3379 3433 Size: 97 1499 463 74 53 31 30 54 Cuts at: 3433 3443 5331 5563 5918 6303 6502 7124 8669 Size: 10 1888 232 355 385 199 622 1545 Cuts at: 8669 1132 Size: 2229 Fragments arranged by size: 2229 1888 1545 1499 622 463 385 355 232 199 97 74 54 53 31 30 10 PasI CC'CwG_GG Cuts at: 1348 4102 4180 4842 1348 Size: 2754 78 662 6272 Fragments arranged by size: 6272 2754 662 78 PciI A'CATG_T Cuts at: 3375 5327 8665 3375 Size: 1952 3338 4476 Fragments arranged by size: 4476 3338 1952 PflMI CCAn_nnn'nTGG Cuts at: 1108 5498 6313 6964 1108 Size: 4390 815 651 3910 Fragments arranged by size: 4390 3910 815 651 PfoI T'CCnGG_A Cuts at: 2269 2299 4568 2269 Size: 30 2269 7467 Fragments arranged by size: 7467 2269 30 PmeI GTTT'AAAC Cuts at: 7070 7070 Size: 9766 PmlI CAC'GTG Cuts at: 5137 5137 Size: 9766 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7835 7867 9348 9380 7835 Size: 32 1481 32 8221 Fragments arranged by size: 8221 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2146 3448 5184 2146 Size: 1302 1736 6728 Fragments arranged by size: 6728 1736 1302 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9766 PsiI TTA'TAA Cuts at: 7336 7336 Size: 9766 PspOMI G'GGCC_C Cuts at: 3862 5501 5980 3862 Size: 1639 479 7648 Fragments arranged by size: 7648 1639 479 PspXI vC'TCGA_Gb Cuts at: 5390 5390 Size: 9766 PstI C_TGCA'G Cuts at: 830 2885 3588 3653 4906 4966 5026 5153 5417 Size: 2055 703 65 1253 60 60 127 264 Cuts at: 5417 5488 5696 5968 7118 830 Size: 71 208 272 1150 3478 Fragments arranged by size: 3478 2055 1253 1150 703 272 264 208 127 71 65 60 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9766 PvuII CAG'CTG Cuts at: 2357 3502 3901 4080 5838 6313 6571 7012 2357 Size: 1145 399 179 1758 475 258 441 5111 Fragments arranged by size: 5111 1758 1145 475 441 399 258 179 SalI G'TCGA_C Cuts at: 1936 7108 1936 Size: 5172 4594 SanDI GG'GwC_CC Cuts at: 2146 5184 2146 Size: 3038 6728 SapI GCTCTTCn'nnn_ Cuts at: 3108 4857 3108 Size: 1749 8017 SbfI CC_TGCA'GG Cuts at: 4906 4966 5026 7118 4906 Size: 60 60 2092 7554 Fragments arranged by size: 7554 2092 60 60 ScaI AGT'ACT Cuts at: 6632 8014 9726 6632 Size: 1382 1712 6672 Fragments arranged by size: 6672 1712 1382 SfcI C'TryA_G Cuts at: 826 1045 2680 2881 3584 3649 4902 4962 5022 Size: 219 1635 201 703 65 1253 60 60 Cuts at: 5022 5149 5413 5484 5516 5692 5794 5964 7114 Size: 127 264 71 32 176 102 170 1150 Cuts at: 7114 8249 8930 9121 826 Size: 1135 681 191 1471 Fragments arranged by size: 1635 1471 1253 1150 1135 703 681 264 219 201 191 176 170 127 102 71 65 60 60 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9766 SgrAI Cr'CCGG_yG Cuts at: 1700 5571 1700 Size: 3871 5895 SmaI CCC'GGG Cuts at: 1872 2144 6173 7093 1872 Size: 272 4029 920 4545 Fragments arranged by size: 4545 4029 920 272 SmlI C'TyrA_G Cuts at: 820 1017 1943 2621 2819 4139 4415 5390 5452 Size: 197 926 678 198 1320 276 975 62 Cuts at: 5452 5579 5906 6065 6488 7870 8771 9033 820 Size: 127 327 159 423 1382 901 262 1553 Fragments arranged by size: 1553 1382 1320 975 926 901 678 423 327 276 262 198 197 159 127 62 SnaBI TAC'GTA Cuts at: 493 7557 493 Size: 7064 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9766 SphI G_CATG'C Cuts at: 1132 2728 3191 5563 5918 7124 1132 Size: 1596 463 2372 355 1206 3774 Fragments arranged by size: 3774 2372 1596 1206 463 355 SspI AAT'ATT Cuts at: 5 52 7690 5 Size: 47 7638 2081 Fragments arranged by size: 7638 2081 47 StuI AGG'CCT Cuts at: 1517 2909 3815 4754 6089 6722 1517 Size: 1392 906 939 1335 633 4561 Fragments arranged by size: 4561 1392 1335 939 906 633 StyI C'CwwG_G Cuts at: 513 1065 2431 2935 3000 3366 3790 4027 4582 Size: 552 1366 504 65 366 424 237 555 Cuts at: 4582 5845 7205 513 Size: 1263 1360 3074 Fragments arranged by size: 3074 1366 1360 1263 555 552 504 424 366 237 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2411 2636 4031 4244 4566 4667 4732 Size: 1255 831 225 1395 213 322 101 65 Cuts at: 4732 4993 5053 6072 6764 6860 7793 7810 7963 Size: 261 60 1019 692 96 933 17 153 Cuts at: 7963 8148 8593 325 Size: 185 445 1498 Fragments arranged by size: 1498 1395 1255 1019 933 831 692 445 322 261 225 213 185 153 101 96 65 60 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5252 6630 8012 Size: 274 80 33 51 1443 3275 1378 1382 Cuts at: 8012 9724 96 Size: 1712 138 Fragments arranged by size: 3275 1712 1443 1382 1378 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2048 2080 3362 3394 3584 3616 3756 3788 4294 Size: 32 1282 32 190 32 140 32 506 Cuts at: 4294 4326 5075 5107 9726 9758 2048 Size: 32 749 32 4619 32 2056 Fragments arranged by size: 4619 2056 1282 749 506 190 140 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4952 5012 1316 Size: 3636 60 6070 Fragments arranged by size: 6070 3636 60 XbaI T'CTAG_A Cuts at: 7102 7102 Size: 9766 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3611 5519 3611 Size: 1908 7858 XhoI C'TCGA_G Cuts at: 1943 5390 1943 Size: 3447 6319 XmaI C'CCGG_G Cuts at: 1870 2142 6171 7091 1870 Size: 272 4029 920 4545 Fragments arranged by size: 4545 4029 920 272 XmnI GAAnn'nnTTC Cuts at: 2600 7895 2600 Size: 5295 4471 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7574 276 Size: 53 83 186 936 6040 2468 Fragments arranged by size: 6040 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI