(Circular) (Six-base) MAPSORT of: pFN21AA0072.seq Check: 9990 from: 1 to: 6217 pFN21AA0072 6217 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2952 3560 1937 Size: 1015 608 4594 Fragments arranged by size: 4594 1015 608 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4280 5664 5932 1632 Size: 2648 1384 268 1917 Fragments arranged by size: 2648 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 2233 3333 5048 2233 Size: 1100 1715 3402 Fragments arranged by size: 3402 1715 1100 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 2641 3960 5532 2641 Size: 1319 1572 3326 Fragments arranged by size: 3326 1572 1319 ApaLI G'TGCA_C Cuts at: 4274 5430 4274 Size: 1156 5061 ApoI r'AATT_y Cuts at: 1493 2341 2760 3526 3752 3904 6013 1493 Size: 848 419 766 226 152 2109 1697 Fragments arranged by size: 2109 1697 848 766 419 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 3180 3542 1870 Size: 73 1237 362 4545 Fragments arranged by size: 4545 1237 362 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2596 2629 2596 Size: 33 6184 BamHI G'GATC_C Cuts at: 2972 3184 3547 3914 2972 Size: 212 363 367 5275 Fragments arranged by size: 5275 367 363 212 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3538 4993 618 Size: 325 217 627 1751 1455 1842 Fragments arranged by size: 1842 1751 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3198 3286 3536 1391 Size: 1807 88 250 4072 Fragments arranged by size: 4072 1807 250 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5246 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2209 4109 5319 2209 Size: 1900 1210 3107 Fragments arranged by size: 3107 1900 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4828 136 Size: 107 122 71 631 605 3156 1525 Fragments arranged by size: 3156 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2868 6212 2868 Size: 3344 2873 BlpI GC'TnA_GC Cuts at: 3634 3634 Size: 6217 Bme1580I G_kGCm'C Cuts at: 1790 4278 5434 1790 Size: 2488 1156 2573 Fragments arranged by size: 2573 2488 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3059 3948 4906 Size: 416 53 83 399 24 1642 889 958 Cuts at: 4906 442 Size: 1753 Fragments arranged by size: 1753 1642 958 889 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2228 2501 3212 4877 1239 Size: 842 147 273 711 1665 2579 Fragments arranged by size: 2579 1665 842 711 273 147 Bpu10I CC'TnA_GC Cuts at: 3123 3123 Size: 6217 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2724 4342 5207 5505 2724 Size: 1618 865 298 3436 Fragments arranged by size: 3436 1618 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4880 882 Size: 617 3381 2219 Fragments arranged by size: 3381 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2242 4008 493 Size: 711 1038 1766 2702 Fragments arranged by size: 2702 1766 1038 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4024 4406 5041 275 Size: 53 83 186 936 2491 382 635 1451 Fragments arranged by size: 2491 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2233 3333 4650 5048 5322 5469 5880 2233 Size: 1100 1317 398 274 147 411 2570 Fragments arranged by size: 2570 1317 1100 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3501 3531 1178 Size: 30 165 30 2098 30 3864 Fragments arranged by size: 3864 2098 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 1186 Size: 892 5325 BseYI C'CCAG_C Cuts at: 2634 5420 5916 2634 Size: 2786 496 2935 Fragments arranged by size: 2935 2786 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5644 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3198 4278 4363 5434 3198 Size: 1080 85 1071 3981 Fragments arranged by size: 3981 1080 1071 85 BsmI GAATG_Cn' Cuts at: 3064 3728 3821 3064 Size: 664 93 5460 Fragments arranged by size: 5460 664 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3198 3286 3536 4278 4363 5434 1391 Size: 399 1408 88 250 742 85 1071 2174 Fragments arranged by size: 2174 1408 1071 742 399 250 88 85 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2274 3572 844 Size: 1430 1298 3489 Fragments arranged by size: 3489 1430 1298 BsrBI CCG'CTC Cuts at: 1394 1823 2104 2149 4104 1394 Size: 429 281 45 1955 3507 Fragments arranged by size: 3507 1955 429 281 45 BsrDI GCAATG_nn' Cuts at: 65 1248 3264 4712 4886 65 Size: 1183 2016 1448 174 1396 Fragments arranged by size: 2016 1448 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2233 3333 4861 5048 1700 Size: 110 423 1100 1528 187 2869 Fragments arranged by size: 2869 1528 1100 423 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 2073 1925 Size: 148 6069 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 2098 794 Size: 1304 4913 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 2629 1184 Size: 1445 4772 BstYI r'GATC_y Cuts at: 1972 2868 2972 3184 3547 3914 4299 4316 5768 Size: 896 104 212 363 367 385 17 1452 Cuts at: 5768 5854 5866 6212 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 896 385 367 363 346 212 104 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2255 2255 Size: 6217 BtgI C'CryG_G Cuts at: 513 1065 2004 3202 513 Size: 552 939 1198 3528 Fragments arranged by size: 3528 1198 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2103 3733 4527 4547 2103 Size: 1630 794 20 3773 Fragments arranged by size: 3773 1630 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2920 2955 529 Size: 35 2356 35 3791 Fragments arranged by size: 3791 2356 35 35 DraI TTT'AAA Cuts at: 2604 3521 2604 Size: 917 5300 DraIII CAC_nnn'GTG Cuts at: 2086 2086 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3261 3664 4553 Size: 54 1425 50 275 18 1431 403 889 Cuts at: 4553 6182 8 Size: 1629 43 Fragments arranged by size: 1629 1431 1425 889 403 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2156 3111 3203 4147 1353 Size: 803 955 92 944 3423 Fragments arranged by size: 3423 955 944 803 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2144 4804 5178 5324 387 Size: 1064 138 555 2660 374 146 1280 Fragments arranged by size: 2660 1280 1064 555 374 146 138 EcoICRI GAG'CTC Cuts at: 3196 3196 Size: 6217 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2228 2501 3212 4280 4877 5664 Size: 393 449 147 273 711 1068 597 787 Cuts at: 5664 5932 1239 Size: 268 1524 Fragments arranged by size: 1524 1068 787 711 597 449 393 273 268 147 EcoO109I rG'GnC_Cy Cuts at: 2926 2926 Size: 6217 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 2027 2057 5364 1678 Size: 349 30 3307 2531 Fragments arranged by size: 3307 2531 349 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2405 2437 2783 2815 2905 Size: 32 135 32 833 32 346 32 90 Cuts at: 2905 2937 4858 4890 4932 4964 1373 Size: 32 1921 32 42 32 2626 Fragments arranged by size: 2626 1921 833 346 135 90 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2263 3561 3807 669 Size: 988 281 325 1298 246 3079 Fragments arranged by size: 3079 1298 988 325 281 246 HindIII A'AGCT_T Cuts at: 748 3354 748 Size: 2606 3611 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3408 3816 3408 Size: 408 5809 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2941 5331 5515 1305 Size: 1636 2390 184 2007 Fragments arranged by size: 2390 2007 1636 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4395 Fragments arranged by size: 4395 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2289 4175 4534 4693 518 Size: 648 1123 1886 359 159 2042 Fragments arranged by size: 2042 1886 1123 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 3633 4310 5458 5703 1606 Size: 453 1574 677 1148 245 2120 Fragments arranged by size: 2120 1574 1148 677 453 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4726 Fragments arranged by size: 4726 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 3575 5120 1132 Size: 97 2346 1545 2229 Fragments arranged by size: 2346 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3279 1348 Size: 1931 4286 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2020 2227 1108 Size: 912 207 5098 Fragments arranged by size: 5098 912 207 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PmlI CAC'GTG Cuts at: 2242 2242 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2926 2926 Size: 6217 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PstI C_TGCA'G Cuts at: 830 2094 2271 3569 830 Size: 1264 177 1298 3478 Fragments arranged by size: 3478 1298 1264 177 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 SacI G_AGCT'C Cuts at: 3198 3198 Size: 6217 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SapI GCTCTTCn'nnn_ Cuts at: 2156 3203 2156 Size: 1047 5170 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 2090 2267 2424 2949 3565 4700 5381 Size: 219 1045 177 157 525 616 1135 681 Cuts at: 5381 5572 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1045 681 616 525 219 191 177 157 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2739 4321 5222 5484 820 Size: 197 926 796 1582 901 262 1553 Fragments arranged by size: 1582 1553 926 901 796 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 3379 152 Size: 3227 2990 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6217 StyI C'CwwG_G Cuts at: 513 1065 2004 3656 513 Size: 552 939 1652 3074 Fragments arranged by size: 3074 1652 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4244 4261 4414 4599 5044 325 Size: 1255 2664 17 153 185 445 1498 Fragments arranged by size: 2664 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3111 4463 6175 Size: 274 80 33 51 1443 1134 1352 1712 Cuts at: 6175 96 Size: 138 Fragments arranged by size: 1712 1443 1352 1134 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6177 6209 6177 Size: 32 6185 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2623 2623 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 2235 4346 2235 Size: 2111 4106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI