(Circular) (Six-base) MAPSORT of: pFN21AA0059.seq Check: 3540 from: 1 to: 6859 pFN21AA0059 6859 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2204 2711 2204 Size: 507 6352 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 4669 278 Size: 53 83 186 936 2086 1047 2468 Fragments arranged by size: 2468 2086 1047 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2952 4202 1937 Size: 1015 1250 4594 Fragments arranged by size: 4594 1250 1015 Acc65I G'GTAC_C Cuts at: 3138 4180 3138 Size: 1042 5817 AclI AA'CG_TT Cuts at: 1563 4986 5359 1563 Size: 3423 373 3063 Fragments arranged by size: 3423 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4083 4922 6306 6574 1632 Size: 2451 839 1384 268 1917 Fragments arranged by size: 2451 1917 1384 839 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6662 AflIII A'CryG_T Cuts at: 1203 3951 5758 6735 1203 Size: 2748 1807 977 1327 Fragments arranged by size: 2748 1807 1327 977 AgeI A'CCGG_T Cuts at: 5690 5690 Size: 6859 AhdI GACnn_n'nnGTC Cuts at: 5588 5588 Size: 6859 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6859 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6859 AlwNI CAG_nnn'CTG Cuts at: 2539 4602 6174 2539 Size: 2063 1572 3224 Fragments arranged by size: 3224 2063 1572 ApaLI G'TGCA_C Cuts at: 2451 2748 4916 6072 2451 Size: 297 2168 1156 3238 Fragments arranged by size: 3238 2168 1156 297 ApoI r'AATT_y Cuts at: 1493 4168 4394 4546 6655 1493 Size: 2675 226 152 2109 1697 Fragments arranged by size: 2675 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3644 5413 160 Size: 872 2612 1769 1606 Fragments arranged by size: 2612 1769 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 AvaI C'yCGr_G Cuts at: 1870 1943 4184 1870 Size: 73 2241 4545 Fragments arranged by size: 4545 2241 73 BamHI G'GATC_C Cuts at: 4189 4556 4189 Size: 367 6492 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3138 3487 4180 5635 618 Size: 325 217 627 1351 349 693 1455 1842 Fragments arranged by size: 1842 1455 1351 693 627 349 325 217 BanII G_rGCy'C Cuts at: 1391 2034 3188 4178 1391 Size: 643 1154 990 4072 Fragments arranged by size: 4072 1154 990 643 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2852 928 Size: 971 953 4935 Fragments arranged by size: 4935 971 953 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5050 5084 1903 Size: 34 3113 34 3678 Fragments arranged by size: 3678 3113 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4751 5961 4751 Size: 1210 5649 BclI T'GATC_A Cuts at: 1548 1548 Size: 6859 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5470 136 Size: 107 122 71 631 605 3798 1525 Fragments arranged by size: 3798 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3028 3578 6854 3028 Size: 550 3276 3033 Fragments arranged by size: 3276 3033 550 BlpI GC'TnA_GC Cuts at: 4276 4276 Size: 6859 Bme1580I G_kGCm'C Cuts at: 1790 2455 2752 4920 6076 1790 Size: 665 297 2168 1156 2573 Fragments arranged by size: 2573 2168 1156 665 297 BmgBI CAC'GTC Cuts at: 1318 3234 1318 Size: 1916 4943 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4590 5548 442 Size: 416 53 83 399 24 3173 958 1753 Fragments arranged by size: 3173 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6859 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2273 2273 Size: 6859 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2476 2735 2893 4064 5519 1239 Size: 1237 259 158 1171 1455 2579 Fragments arranged by size: 2579 1455 1237 1171 259 158 Bpu10I CC'TnA_GC Cuts at: 3342 3342 Size: 6859 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4984 5849 6147 4984 Size: 865 298 5696 Fragments arranged by size: 5696 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2400 5522 882 Size: 617 901 3122 2219 Fragments arranged by size: 3122 2219 901 617 BsaAI yAC'GTr Cuts at: 493 1204 4650 493 Size: 711 3446 2702 Fragments arranged by size: 3446 2702 711 BsaBI GATnn'nnATC Cuts at: 6853 6853 Size: 6859 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3619 4666 5048 5683 Size: 53 83 186 936 2086 1047 382 635 Cuts at: 5683 275 Size: 1451 Fragments arranged by size: 2086 1451 1047 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5292 5690 5964 6111 6522 5292 Size: 398 274 147 411 5629 Fragments arranged by size: 5629 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2397 2427 3100 3130 1178 Size: 30 165 30 994 30 673 30 4907 Fragments arranged by size: 4907 994 673 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3948 1186 Size: 2762 4097 BseYI C'CCAG_C Cuts at: 2741 3246 3475 3497 6062 6558 2741 Size: 505 229 22 2565 496 3042 Fragments arranged by size: 3042 2565 505 496 229 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2245 1163 Size: 573 509 5777 Fragments arranged by size: 5777 573 509 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4309 5070 6098 1540 Size: 275 186 1285 1023 761 1028 2301 Fragments arranged by size: 2301 1285 1028 1023 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2455 2635 2752 4920 5005 6076 2034 Size: 421 180 117 2168 85 1071 2817 Fragments arranged by size: 2817 2168 1071 421 180 117 85 BsmI GAATG_Cn' Cuts at: 4370 4463 4370 Size: 93 6766 BsmBI CGTCTCn'nnnn_ Cuts at: 3512 3610 4117 3512 Size: 98 507 6254 Fragments arranged by size: 6254 507 98 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2455 2635 2752 3188 4178 4920 Size: 399 244 421 180 117 436 990 742 Cuts at: 4920 5005 6076 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 990 742 436 421 399 244 180 117 85 BspEI T'CCGG_A Cuts at: 6522 6522 Size: 6859 BspHI T'CATG_A Cuts at: 4748 6478 4748 Size: 1730 5129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2204 2711 4214 844 Size: 1360 507 1503 3489 Fragments arranged by size: 3489 1503 1360 507 BsrBI CCG'CTC Cuts at: 1394 1823 3240 4746 1394 Size: 429 1417 1506 3507 Fragments arranged by size: 3507 1506 1417 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3274 3924 5354 5528 65 Size: 1183 2026 650 1430 174 1396 Fragments arranged by size: 2026 1430 1396 1183 650 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2504 3306 3819 5503 5690 1700 Size: 110 694 802 513 1684 187 2869 Fragments arranged by size: 2869 1684 802 694 513 187 110 BsrGI T'GTAC_A Cuts at: 96 3204 96 Size: 3108 3751 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6859 BssSI C'ACGA_G Cuts at: 3175 4919 5931 3175 Size: 1744 1012 4103 Fragments arranged by size: 4103 1744 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2539 2589 2763 794 Size: 1745 50 174 4890 Fragments arranged by size: 4890 1745 174 50 BstBI TT'CG_AA Cuts at: 5663 5663 Size: 6859 BstEII G'GTnAC_C Cuts at: 1184 3991 4110 1184 Size: 2807 119 3933 Fragments arranged by size: 3933 2807 119 BstXI CCAn_nnnn'nTGG Cuts at: 2129 2306 3263 3533 3955 2129 Size: 177 957 270 422 5033 Fragments arranged by size: 5033 957 422 270 177 BstYI r'GATC_y Cuts at: 1972 2961 3028 3578 4189 4556 4941 4958 6410 Size: 989 67 550 611 367 385 17 1452 Cuts at: 6410 6496 6508 6854 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 989 611 550 385 367 346 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2079 2079 Size: 6859 BtgI C'CryG_G Cuts at: 513 1065 2052 2299 513 Size: 552 987 247 5073 Fragments arranged by size: 5073 987 552 247 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6120 Fragments arranged by size: 6120 683 56 BtsI GCAGTG_nn' Cuts at: 2116 2564 4375 5169 5189 2116 Size: 448 1811 794 20 3786 Fragments arranged by size: 3786 1811 794 448 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6859 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2188 2223 529 Size: 35 1624 35 5165 Fragments arranged by size: 5165 1624 35 35 DraI TTT'AAA Cuts at: 2578 4163 2578 Size: 1585 5274 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5866 809 Size: 507 4550 1802 Fragments arranged by size: 4550 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3283 3308 3904 Size: 54 1425 50 275 18 1453 25 596 Cuts at: 3904 4306 5195 6824 8 Size: 402 889 1629 43 Fragments arranged by size: 1629 1453 1425 889 596 402 275 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 4306 1537 Size: 275 1471 1023 4090 Fragments arranged by size: 4090 1471 1023 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2509 3630 3681 4160 4789 1353 Size: 1156 1121 51 479 629 3423 Fragments arranged by size: 3423 1156 1121 629 479 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5446 5820 5966 387 Size: 1064 138 3857 374 146 1280 Fragments arranged by size: 3857 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2032 2032 Size: 6859 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2476 2735 2893 4064 4083 4922 5519 Size: 393 844 259 158 1171 19 839 597 Cuts at: 5519 6306 6574 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1171 844 839 787 597 393 268 259 158 19 EcoNI CCTnn'n_nnAGG Cuts at: 4022 4022 Size: 6859 EcoO109I rG'GnC_Cy Cuts at: 2083 2599 3346 3560 2083 Size: 516 747 214 5382 Fragments arranged by size: 5382 747 516 214 EcoRI G'AATT_C Cuts at: 4168 4546 4168 Size: 378 6481 EcoRV GAT'ATC Cuts at: 2521 3454 2521 Size: 933 5926 FspI TGC'GCA Cuts at: 5365 5365 Size: 6859 HaeII r_GCGC'y Cuts at: 1678 2392 2540 2549 4051 6006 1678 Size: 714 148 9 1502 1955 2531 Fragments arranged by size: 2531 1955 1502 714 148 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2341 2373 2397 2429 3100 Size: 32 135 32 769 32 24 32 671 Cuts at: 3100 3132 3151 3183 3660 3692 5500 5532 5574 Size: 32 19 32 477 32 1808 32 42 Cuts at: 5574 5606 1373 Size: 32 2626 Fragments arranged by size: 2626 1808 769 671 477 135 42 32 32 32 32 32 32 32 32 32 24 19 HincII GTy'rAC Cuts at: 669 1657 1938 2444 4203 4449 669 Size: 988 281 506 1759 246 3079 Fragments arranged by size: 3079 1759 988 506 281 246 HindIII A'AGCT_T Cuts at: 748 3650 748 Size: 2902 3957 HpaI GTT'AAC Cuts at: 4449 4449 Size: 6859 KpnI G_GTAC'C Cuts at: 3142 4184 3142 Size: 1042 5817 MfeI C'AATT_G Cuts at: 2362 2497 4458 2362 Size: 135 1961 4763 Fragments arranged by size: 4763 1961 135 MluI A'CGCG_T Cuts at: 6735 6735 Size: 6859 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3251 3356 3936 5973 6157 1305 Size: 1946 105 580 2037 184 2007 Fragments arranged by size: 2037 2007 1946 580 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 3906 10 Size: 54 1425 343 2074 2963 Fragments arranged by size: 2963 2074 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2127 2304 3360 4817 5176 5335 Size: 648 843 118 177 1056 1457 359 159 Cuts at: 5335 518 Size: 2042 Fragments arranged by size: 2042 1457 1056 843 648 359 177 159 118 MspA1I CmG'CkG Cuts at: 1606 2278 2745 3282 3870 4275 4952 6100 6345 Size: 672 467 537 588 405 677 1148 245 Cuts at: 6345 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 672 588 537 467 405 245 NaeI GCC'GGC Cuts at: 2506 2506 Size: 6859 NcoI C'CATG_G Cuts at: 513 1065 2052 2299 513 Size: 552 987 247 5073 Fragments arranged by size: 5073 987 552 247 NdeI CA'TA_TG Cuts at: 387 3976 387 Size: 3589 3270 NgoMIV G'CCGG_C Cuts at: 2504 2504 Size: 6859 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6859 NotI GC'GGCC_GC Cuts at: 4306 4306 Size: 6859 NspI r_CATG'y Cuts at: 1132 1229 3419 3913 3955 4217 5762 1132 Size: 97 2190 494 42 262 1545 2229 Fragments arranged by size: 2229 2190 1545 494 262 97 42 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6859 PciI A'CATG_T Cuts at: 3951 5758 3951 Size: 1807 5052 PflMI CCAn_nnn'nTGG Cuts at: 1108 2817 1108 Size: 1709 5150 PfoI T'CCnGG_A Cuts at: 2712 2964 2712 Size: 252 6607 PmeI GTTT'AAAC Cuts at: 4163 4163 Size: 6859 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4928 4960 6441 6473 4928 Size: 32 1481 32 5314 Fragments arranged by size: 5314 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2599 3560 2083 Size: 516 961 5382 Fragments arranged by size: 5382 961 516 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6859 PsiI TTA'TAA Cuts at: 4429 4429 Size: 6859 PstI C_TGCA'G Cuts at: 830 4211 830 Size: 3381 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6859 PvuII CAG'CTG Cuts at: 2745 2745 Size: 6859 SacI G_AGCT'C Cuts at: 2034 2034 Size: 6859 SalI G'TCGA_C Cuts at: 1936 4201 1936 Size: 2265 4594 SapI GCTCTTCn'nnn_ Cuts at: 3630 3630 Size: 6859 SbfI CC_TGCA'GG Cuts at: 4211 4211 Size: 6859 ScaI AGT'ACT Cuts at: 5107 6819 5107 Size: 1712 5147 SexAI A'CCwGG_T Cuts at: 2926 2926 Size: 6859 SfcI C'TryA_G Cuts at: 826 1045 4207 5342 6023 6214 826 Size: 219 3162 1135 681 191 1471 Fragments arranged by size: 3162 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6859 SmaI CCC'GGG Cuts at: 1872 4186 1872 Size: 2314 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4963 5864 6126 820 Size: 197 926 3020 901 262 1553 Fragments arranged by size: 3020 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4650 493 Size: 4157 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6859 SphI G_CATG'C Cuts at: 1132 3419 4217 1132 Size: 2287 798 3774 Fragments arranged by size: 3774 2287 798 SspI AAT'ATT Cuts at: 5 52 4783 5 Size: 47 4731 2081 Fragments arranged by size: 4731 2081 47 StuI AGG'CCT Cuts at: 1517 3663 1517 Size: 2146 4713 StyI C'CwwG_G Cuts at: 513 1065 2016 2052 2299 3091 3423 4298 513 Size: 552 951 36 247 792 332 875 3074 Fragments arranged by size: 3074 951 875 792 552 332 247 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2109 2424 2864 3159 3415 3945 4886 Size: 1255 529 315 440 295 256 530 941 Cuts at: 4886 4903 5056 5241 5686 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 941 530 529 445 440 315 295 256 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2375 2613 3204 Size: 274 80 33 51 1443 398 238 591 Cuts at: 3204 5105 6817 96 Size: 1901 1712 138 Fragments arranged by size: 1901 1712 1443 591 398 274 238 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 6819 6851 2543 Size: 32 4244 32 2551 Fragments arranged by size: 4244 2551 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2411 3112 1316 Size: 1095 701 5063 Fragments arranged by size: 5063 1095 701 XbaI T'CTAG_A Cuts at: 4195 4195 Size: 6859 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2663 2663 Size: 6859 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6859 XmaI C'CCGG_G Cuts at: 1870 4184 1870 Size: 2314 4545 XmnI GAAnn'nnTTC Cuts at: 2154 4068 4988 2154 Size: 1914 920 4025 Fragments arranged by size: 4025 1914 920 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 4667 276 Size: 53 83 186 936 2086 1047 2468 Fragments arranged by size: 2468 2086 1047 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI