Restriction map for
pFN21AA0030
(Linear) (Six-base) MAPSORT of: KIAA0030.seq Check: 2035 from: 1 to: 2712
KIAA0030.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 869 2517 2712
Size: 869 1648 195
Fragments arranged by size:
1648 869 195
AatII G_ACGT'C
Cuts at: 0 2372 2712
Size: 2372 340
AccI GT'mk_AC
Cuts at: 0 620 2712
Size: 620 2092
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2179 2527 2640 2712
Size: 2179 348 113 72
Fragments arranged by size:
2179 348 113 72
AfeI AGC'GCT
Cuts at: 0 2100 2712
Size: 2100 612
AflIII A'CryG_T
Cuts at: 0 662 2123 2712
Size: 662 1461 589
Fragments arranged by size:
1461 662 589
AhdI GACnn_n'nnGTC
Cuts at: 0 320 2712
Size: 320 2392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2359 2712
Size: 2359 353
ApaI G_GGCC'C
Cuts at: 0 185 571 1725 2559 2712
Size: 185 386 1154 834 153
Fragments arranged by size:
1154 834 386 185 153
ApoI r'AATT_y
Cuts at: 0 1762 2712
Size: 1762 950
AvrII C'CTAG_G
Cuts at: 0 168 2712
Size: 168 2544
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1107 1140 2712
Size: 1107 33 1572
Fragments arranged by size:
1572 1107 33
BanI G'GyrC_C
Cuts at: 0 750 1685 2712
Size: 750 935 1027
Fragments arranged by size:
1027 935 750
BanII G_rGCy'C
Cuts at: 0 167 185 215 404 571 1511 1725 1955
Size: 167 18 30 189 167 940 214 230
Cuts at: 1955 2111 2141 2559 2654 2712
Size: 156 30 418 95 58
Fragments arranged by size:
940 418 230 214 189 167 167 156
95 58 30 30 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 633 1472 2369 2712
Size: 633 839 897 343
Fragments arranged by size:
897 839 633 343
BbvCI CC'TCA_GC
Cuts at: 0 889 2712
Size: 889 1823
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 600 634 2712
Size: 600 34 2078
Fragments arranged by size:
2078 600 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1135 2712
Size: 1135 1577
BclI T'GATC_A
Cuts at: 0 1388 2712
Size: 1388 1324
BglI GCCn_nnn'nGGC
Cuts at: 0 271 2712
Size: 271 2441
BglII A'GATC_T
Cuts at: 0 770 1200 1427 2712
Size: 770 430 227 1285
Fragments arranged by size:
1285 770 430 227
BlpI GC'TnA_GC
Cuts at: 0 964 2105 2712
Size: 964 1141 607
Fragments arranged by size:
1141 964 607
Bme1580I G_kGCm'C
Cuts at: 0 185 571 1725 2559 2712
Size: 185 386 1154 834 153
Fragments arranged by size:
1154 834 386 185 153
BmgBI CAC'GTC
Cuts at: 0 618 2049 2712
Size: 618 1431 663
Fragments arranged by size:
1431 663 618
BmrI ACTGGGnnnn_n'
Cuts at: 0 1999 2712
Size: 1999 713
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 229 900 1768 2712
Size: 229 671 868 944
Fragments arranged by size:
944 868 671 229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 102 208 541 598 811 2416 2712
Size: 60 42 106 333 57 213 1605 296
Fragments arranged by size:
1605 333 296 213 106 60 57 42
Bpu10I CC'TnA_GC
Cuts at: 0 889 2712
Size: 889 1823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1582 2712
Size: 1582 1130
BsaI GGTCTCn'nnnn_
Cuts at: 0 1093 1562 2712
Size: 1093 469 1150
Fragments arranged by size:
1150 1093 469
BsaAI yAC'GTr
Cuts at: 0 726 1329 1540 2126 2712
Size: 726 603 211 586 586
Fragments arranged by size:
726 603 586 586 211
BsaBI GATnn'nnATC
Cuts at: 0 2617 2712
Size: 2617 95
BsaHI Gr'CG_yC
Cuts at: 0 263 950 1184 1651 2369 2712
Size: 263 687 234 467 718 343
Fragments arranged by size:
718 687 467 343 263 234
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 322 436 445 499 508 889 1111
Size: 223 99 114 9 54 9 381 222
Cuts at: 1111 1183 1757 2089 2092 2712
Size: 72 574 332 3 620
Fragments arranged by size:
620 574 381 332 223 222 114 99
72 54 9 9 3
BseYI C'CCAG_C
Cuts at: 0 44 959 1717 2062 2558 2712
Size: 44 915 758 345 496 154
Fragments arranged by size:
915 758 496 345 154 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 1854 2712
Size: 927 927 858
Fragments arranged by size:
927 927 858
BsiEI CG_ry'CG
Cuts at: 0 825 1073 2712
Size: 825 248 1639
Fragments arranged by size:
1639 825 248
BsiHKAI G_wGCw'C
Cuts at: 0 215 725 1559 2654 2712
Size: 215 510 834 1095 58
Fragments arranged by size:
1095 834 510 215 58
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1220 2712
Size: 1220 1492
Bsp1286I G_dGCh'C
Cuts at: 0 167 185 215 404 571 725 1511 1559
Size: 167 18 30 189 167 154 786 48
Cuts at: 1559 1725 1955 2111 2141 2559 2654 2712
Size: 166 230 156 30 418 95 58
Fragments arranged by size:
786 418 230 189 167 167 166 156
154 95 58 48 30 30 18
BspHI T'CATG_A
Cuts at: 0 1461 2712
Size: 1461 1251
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 869 2517 2712
Size: 869 1648 195
Fragments arranged by size:
1648 869 195
BsrBI CCG'CTC
Cuts at: 0 342 465 1175 2712
Size: 342 123 710 1537
Fragments arranged by size:
1537 710 342 123
BsrDI GCAATG_nn'
Cuts at: 0 66 1439 1877 2712
Size: 66 1373 438 835
Fragments arranged by size:
1373 835 438 66
BsrFI r'CCGG_y
Cuts at: 0 753 1072 1163 2712
Size: 753 319 91 1549
Fragments arranged by size:
1549 753 319 91
BsrGI T'GTAC_A
Cuts at: 0 2237 2712
Size: 2237 475
BssHII G'CGCG_C
Cuts at: 0 2332 2712
Size: 2332 380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 992 2712
Size: 992 1720
BstEII G'GTnAC_C
Cuts at: 0 933 2712
Size: 933 1779
BstXI CCAn_nnnn'nTGG
Cuts at: 0 930 1329 2712
Size: 930 399 1383
Fragments arranged by size:
1383 930 399
BstYI r'GATC_y
Cuts at: 0 525 581 770 1200 1427 2712
Size: 525 56 189 430 227 1285
Fragments arranged by size:
1285 525 430 227 189 56
Bsu36I CC'TnA_GG
Cuts at: 0 746 2248 2712
Size: 746 1502 464
Fragments arranged by size:
1502 746 464
BtgI C'CryG_G
Cuts at: 0 26 396 470 627 1808 1997 2712
Size: 26 370 74 157 1181 189 715
Fragments arranged by size:
1181 715 370 189 157 74 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 431 2712
Size: 431 2281
BtsI GCAGTG_nn'
Cuts at: 0 1280 2712
Size: 1280 1432
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1521 1556 2712
Size: 1521 35 1156
Fragments arranged by size:
1521 1156 35
DraIII CAC_nnn'GTG
Cuts at: 0 543 2712
Size: 543 2169
EaeI y'GGCC_r
Cuts at: 0 84 126 317 372 630 803 1070 1161
Size: 84 42 191 55 258 173 267 91
Cuts at: 1161 1331 1641 2087 2228 2378 2712
Size: 170 310 446 141 150 334
Fragments arranged by size:
446 334 310 267 258 191 173 170
150 141 91 84 55 42
EagI C'GGCC_G
Cuts at: 0 1070 2712
Size: 1070 1642
EarI CTCTTCn'nnn_
Cuts at: 0 191 2503 2659 2712
Size: 191 2312 156 53
Fragments arranged by size:
2312 191 156 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 772 2335 2712
Size: 19 753 1563 377
Fragments arranged by size:
1563 753 377 19
EcoICRI GAG'CTC
Cuts at: 0 213 2652 2712
Size: 213 2439 60
Fragments arranged by size:
2439 213 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 102 208 541 598 811 2179 2416
Size: 60 42 106 333 57 213 1368 237
Cuts at: 2416 2527 2640 2712
Size: 111 113 72
Fragments arranged by size:
1368 333 237 213 113 111 106 72
60 57 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 745 1402 2146 2712
Size: 745 657 744 566
Fragments arranged by size:
745 744 657 566
EcoO109I rG'GnC_Cy
Cuts at: 0 181 182 378 568 1009 1721 1722 2154
Size: 181 1 196 190 441 712 1 432
Cuts at: 2154 2555 2577 2712
Size: 401 22 135
Fragments arranged by size:
712 441 432 401 196 190 181 135
22 1 1
EcoRV GAT'ATC
Cuts at: 0 1548 2712
Size: 1548 1164
FseI GG_CCGG'CC
Cuts at: 0 1076 2712
Size: 1076 1636
FspI TGC'GCA
Cuts at: 0 610 2434 2443 2712
Size: 610 1824 9 269
Fragments arranged by size:
1824 610 269 9
HaeII r_GCGC'y
Cuts at: 0 438 453 1908 2102 2712
Size: 438 15 1455 194 610
Fragments arranged by size:
1455 610 438 194 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 306 338 1051 1083 1092 1124
Size: 139 32 135 32 713 32 9 32
Cuts at: 1124 1412 1444 1600 1632 2712
Size: 288 32 156 32 1080
Fragments arranged by size:
1080 713 288 156 139 135 32 32
32 32 32 32 9
HincII GTy'rAC
Cuts at: 0 621 1089 2373 2712
Size: 621 468 1284 339
Fragments arranged by size:
1284 621 468 339
MscI TGG'CCA
Cuts at: 0 805 1333 1643 2089 2230 2380 2712
Size: 805 528 310 446 141 150 332
Fragments arranged by size:
805 528 446 332 310 150 141
MslI CAynn'nnrTG
Cuts at: 0 842 2287 2386 2712
Size: 842 1445 99 326
Fragments arranged by size:
1445 842 326 99
MspA1I CmG'CkG
Cuts at: 0 897 912 941 1214 1683 2712
Size: 897 15 29 273 469 1029
Fragments arranged by size:
1029 897 469 273 29 15
NaeI GCC'GGC
Cuts at: 0 1074 1165 2712
Size: 1074 91 1547
Fragments arranged by size:
1547 1074 91
NcoI C'CATG_G
Cuts at: 0 26 1808 2712
Size: 26 1782 904
Fragments arranged by size:
1782 904 26
NgoMIV G'CCGG_C
Cuts at: 0 1072 1163 2712
Size: 1072 91 1549
Fragments arranged by size:
1549 1072 91
NspI r_CATG'y
Cuts at: 0 393 666 2442 2712
Size: 393 273 1776 270
Fragments arranged by size:
1776 393 273 270
PciI A'CATG_T
Cuts at: 0 662 2712
Size: 662 2050
PfoI T'CCnGG_A
Cuts at: 0 1133 2009 2712
Size: 1133 876 703
Fragments arranged by size:
1133 876 703
PmlI CAC'GTG
Cuts at: 0 726 1329 2712
Size: 726 603 1383
Fragments arranged by size:
1383 726 603
PpuMI rG'GwC_Cy
Cuts at: 0 1009 2154 2577 2712
Size: 1009 1145 423 135
Fragments arranged by size:
1145 1009 423 135
PspOMI G'GGCC_C
Cuts at: 0 181 567 1721 2555 2712
Size: 181 386 1154 834 157
Fragments arranged by size:
1154 834 386 181 157
PstI C_TGCA'G
Cuts at: 0 770 1862 2705 2712
Size: 770 1092 843 7
Fragments arranged by size:
1092 843 770 7
PvuII CAG'CTG
Cuts at: 0 897 912 941 1214 2712
Size: 897 15 29 273 1498
Fragments arranged by size:
1498 897 273 29 15
SacI G_AGCT'C
Cuts at: 0 215 2654 2712
Size: 215 2439 58
Fragments arranged by size:
2439 215 58
SalI G'TCGA_C
Cuts at: 0 619 2712
Size: 619 2093
SapI GCTCTTCn'nnn_
Cuts at: 0 2503 2659 2712
Size: 2503 156 53
Fragments arranged by size:
2503 156 53
SbfI CC_TGCA'GG
Cuts at: 0 1862 2712
Size: 1862 850
SfcI C'TryA_G
Cuts at: 0 766 1355 1858 2701 2712
Size: 766 589 503 843 11
Fragments arranged by size:
843 766 589 503 11
SgrAI Cr'CCGG_yG
Cuts at: 0 753 2712
Size: 753 1959
SmlI C'TyrA_G
Cuts at: 0 1597 2712
Size: 1597 1115
SphI G_CATG'C
Cuts at: 0 393 2442 2712
Size: 393 2049 270
Fragments arranged by size:
2049 393 270
StuI AGG'CCT
Cuts at: 0 1481 2712
Size: 1481 1231
StyI C'CwwG_G
Cuts at: 0 26 168 1178 1403 1709 1808 2180 2712
Size: 26 142 1010 225 306 99 372 532
Fragments arranged by size:
1010 532 372 306 225 142 99 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 543 1384 1754 2712
Size: 543 841 370 958
Fragments arranged by size:
958 841 543 370
TatI w'GTAC_w
Cuts at: 0 977 2165 2237 2712
Size: 977 1188 72 475
Fragments arranged by size:
1188 977 475 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2287 2319 2712
Size: 2287 32 393
Fragments arranged by size:
2287 393 32
Tth111I GACn'n_nGTC
Cuts at: 0 2005 2368 2712
Size: 2005 363 344
Fragments arranged by size:
2005 363 344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 255 2313 2712
Size: 255 2058 399
Fragments arranged by size:
2058 399 255
XmnI GAAnn'nnTTC
Cuts at: 0 2668 2712
Size: 2668 44
ZraI GAC'GTC
Cuts at: 0 2370 2712
Size: 2370 342
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AlfI
ApaI ApoI AvrII BaeI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FseI FspI HaeII Hin4I HincII MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PciI PfoI
PmlI PpuMI PspOMI PstI PvuII SacI SalI SapI
SbfI SfcI SgrAI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AleI AloI AlwNI ApaLI
AscI AseI AsiSI AvaI BamHI BbeI BfrBI BmtI
BsaWI BsaXI BsiWI BsmI BspEI BssSI BstBI BstZ17I
ClaI DraI DrdI EcoRI FalI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI NarI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SanDI
ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI
SrfI SspI SwaI XbaI XhoI XmaI