Restriction map for
pFN21AA0012
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BanII BbeI BbrPI BbvCI BcgI
BciVI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FauI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AflII 2313 CTTAAG C^TTAAG
AflIII 1712 ACATGT A^C[GA][CT]GT
AlwNI 995 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 541 CCTGAGG CC^T[ACGT]AGG
AseI 2326 ATTAAT AT^TAAT
AsnI 2326 ATTAAT AT^TAAT
AspHI 438 GAGCAC G[AT]GC[AT]^C
BalI 2249 TGGCCA TGG^CCA
BamHI 788 GGATCC G^GATCC
BfrI 2313 CTTAAG C^TTAAG
BsaI 103 GGTCTC GGTCTC(1/5)
BsgI 574 CTGCAC GTGCAG(16/14)
BsiHKAI 438 GAGCAC G[AT]GC[AT]^C
BsmI 1376 GCATTC GAATGC(1/-1)
BspLU11I 1712 ACATGT A^CATGT
BspMI 2053 ACCTGC ACCTGC(4/8)
BstAPI 373 GCATTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 969 TTCGAA TT^CGAA
BstXI 1763 CCACCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 541 CCTGAGG CC^T[ACGT]AGG
CfoI 1860 GCGC GCG^C
DraI 1281 TTTAAA TTT^AAA
DraII 545 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 577 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2249 TGGCCA [CT]^GGCC[GA]
EarI 2064 GAAGAG CTCTTC(1/4)
Eco81I 541 CCTGAGG CC^T[ACGT]AGG
EcoNI 1695 CCTACTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 545 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 253 GATATC GAT^ATC
EcoT14I 2276 CCAAGG C^C[AT][AT]GG
HhaI 1860 GCGC GCG^C
HinP1I 1860 GCGC G^CGC
HindIII 1403 AAGCTT A^AGCTT
Ksp632I 2064 GAAGAG CTCTTC(1/4)
MluNI 2249 TGGCCA TGG^CCA
MscI 2249 TGGCCA TGG^CCA
NspV 969 TTCGAA TT^CGAA
PflMI 276 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 570 GAGTC GAGTC(4/5)
PshBI 2326 ATTAAT AT^TAAT
PstI 1800 CTGCAG CTGCA^G
ScaI 2207 AGTACT AGT^ACT
SfuI 969 TTCGAA TT^CGAA
SspI 2329 AATATT AAT^ATT
StuI 899 AGGCCT AGG^CCT
StyI 2276 CCAAGG C^C[AT][AT]GG
TaqI 970 TCGA T^CGA
TthHB8I 970 TCGA T^CGA
Van91I 276 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1766 CCATGCTGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
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AciI 943 CCGC CCGC(-3/-1)
2203 CCGC
Asp700I 774 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2194 GAACCCATTC
BanI 1106 GGCACC G^G[CT][GA]CC
2117 GGTGCC
BbsI 535 GAAGAC GAAGAC(2/6)
1290 GTCTTC
BclI 1484 TGATCA T^GATCA
1491 TGATCA
BcnI 1674 CCCGG CC^[GC]GG
1856 CCCGG
BpmI 793 CTCCAG CTGGAG(16/14)
1903 CTCCAG
BpuAI 535 GAAGAC GAAGAC(2/6)
1290 GTCTTC
BsmFI 1565 GGGAC GGGAC(10/14)
300 GTCCC
BspHI 2004 TCATGA T^CATGA
2157 TCATGA
Cac8I 1646 GCTGGC GC[ACGT]^[ACGT]GC
2346 GCAAGC
EcoT22I 371 ATGCAT ATGCA^T
1913 ATGCAT
FbaI 1484 TGATCA T^GATCA
1491 TGATCA
HapII 1675 CCGG C^CGG
1857 CCGG
HpaII 1675 CCGG C^CGG
1857 CCGG
MfeI 473 CAATTG C^AATTG
1204 CAATTG
MslI 1574 CATTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1767 CATGCTGGTG
MspA1I 796 CAGCTG C[AC]G^C[GT]G
856 CAGCTG
MspI 1675 CCGG C^CGG
1857 CCGG
MunI 473 CAATTG C^AATTG
1204 CAATTG
NciI 1674 CCCGG CC^[GC]GG
1856 CCCGG
NsiI 371 ATGCAT ATGCA^T
1913 ATGCAT
NspI 1019 ACATGC [GA]CATG^[CT]
1712 ACATGT
PvuII 796 CAGCTG CAG^CTG
856 CAGCTG
RcaI 2004 TCATGA T^CATGA
2157 TCATGA
SfaNI 379 GATGC GCATC(5/9)
8 GCATC
SmlI 346 CTCAAG C^T[CT][GA]AG
2313 CTTAAG
XmnI 774 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2194 GAACCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EcoRI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII