(Circular) (Six-base) MAPSORT of: pFN21AA0012.seq Check: 8677 from: 1 to: 7063 pFN21AA0012 7063 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4067 4067 Size: 7063 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4873 278 Size: 53 83 186 936 3337 2468 Fragments arranged by size: 3337 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4406 1937 Size: 2469 4594 Acc65I G'GTAC_C Cuts at: 4384 4384 Size: 7063 AclI AA'CG_TT Cuts at: 1563 5190 5563 1563 Size: 3627 373 3063 Fragments arranged by size: 3627 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2033 2091 2854 5126 6510 6778 1632 Size: 401 58 763 2272 1384 268 1917 Fragments arranged by size: 2272 1917 1384 763 401 268 58 AflII C'TTAA_G Cuts at: 820 1017 4318 820 Size: 197 3301 3565 Fragments arranged by size: 3565 3301 197 AflIII A'CryG_T Cuts at: 1203 3717 5962 6939 1203 Size: 2514 2245 977 1327 Fragments arranged by size: 2514 2245 1327 977 AgeI A'CCGG_T Cuts at: 5894 5894 Size: 7063 AhdI GACnn_n'nnGTC Cuts at: 5792 5792 Size: 7063 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7063 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2522 1184 Size: 1338 5725 AlwNI CAG_nnn'CTG Cuts at: 3005 4806 6378 3005 Size: 1801 1572 3690 Fragments arranged by size: 3690 1801 1572 ApaLI G'TGCA_C Cuts at: 5120 6276 5120 Size: 1156 5907 ApoI r'AATT_y Cuts at: 1493 2081 2273 2429 2606 2779 2992 3226 3605 Size: 588 192 156 177 173 213 234 379 Cuts at: 3605 4372 4598 4750 6859 1493 Size: 767 226 152 2109 1697 Fragments arranged by size: 2109 1697 767 588 379 234 226 213 192 177 173 156 152 AseI AT'TA_AT Cuts at: 160 1032 4332 5617 160 Size: 872 3300 1285 1606 Fragments arranged by size: 3300 1606 1285 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7063 AvaI C'yCGr_G Cuts at: 1870 1943 4388 1870 Size: 73 2445 4545 Fragments arranged by size: 4545 2445 73 BamHI G'GATC_C Cuts at: 2793 4393 4760 2793 Size: 1600 367 5096 Fragments arranged by size: 5096 1600 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3111 4122 4384 5839 618 Size: 325 217 627 1324 1011 262 1455 1842 Fragments arranged by size: 1842 1455 1324 1011 627 325 262 217 BanII G_rGCy'C Cuts at: 1391 4382 1391 Size: 2991 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2547 3288 928 Size: 971 648 741 4703 Fragments arranged by size: 4703 971 741 648 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5254 5288 1903 Size: 34 3317 34 3678 Fragments arranged by size: 3678 3317 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4955 6165 4955 Size: 1210 5853 BclI T'GATC_A Cuts at: 1548 3489 3496 1548 Size: 1941 7 5115 Fragments arranged by size: 5115 1941 7 BfrBI ATG'CAT Cuts at: 2378 3920 2378 Size: 1542 5521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5674 136 Size: 107 122 71 631 605 4002 1525 Fragments arranged by size: 4002 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7058 7058 Size: 7063 BlpI GC'TnA_GC Cuts at: 4480 4480 Size: 7063 Bme1580I G_kGCm'C Cuts at: 1790 3114 3942 5124 6280 1790 Size: 1324 828 1182 1156 2573 Fragments arranged by size: 2573 1324 1182 1156 828 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7063 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4794 5752 442 Size: 416 53 83 399 24 3377 958 1753 Fragments arranged by size: 3377 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7063 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2783 3893 5723 1239 Size: 1544 1110 1830 2579 Fragments arranged by size: 2579 1830 1544 1110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 5188 6053 6351 2336 Size: 2852 865 298 3048 Fragments arranged by size: 3048 2852 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2114 5726 882 Size: 617 615 3612 2219 Fragments arranged by size: 3612 2219 617 615 BsaAI yAC'GTr Cuts at: 493 1204 4854 493 Size: 711 3650 2702 Fragments arranged by size: 3650 2702 711 BsaBI GATnn'nnATC Cuts at: 7057 7057 Size: 7063 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4870 5252 5887 275 Size: 53 83 186 936 3337 382 635 1451 Fragments arranged by size: 3337 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5496 5894 6168 6315 6726 5496 Size: 398 274 147 411 5833 Fragments arranged by size: 5833 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3594 3624 1178 Size: 30 165 30 2191 30 4617 Fragments arranged by size: 4617 2191 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7063 BseYI C'CCAG_C Cuts at: 6266 6762 6266 Size: 496 6567 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2564 1163 Size: 573 828 5662 Fragments arranged by size: 5662 828 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4513 5274 6302 1540 Size: 275 186 2512 761 1028 2301 Fragments arranged by size: 2512 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2447 5124 5209 6280 2447 Size: 2677 85 1071 3230 Fragments arranged by size: 3230 2677 1071 85 BsmI GAATG_Cn' Cuts at: 3381 4574 4667 3381 Size: 1193 93 5777 Fragments arranged by size: 5777 1193 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2447 3114 3942 4382 5124 5209 6280 Size: 399 657 667 828 440 742 85 1071 Cuts at: 6280 1391 Size: 2174 Fragments arranged by size: 2174 1071 828 742 667 657 440 399 85 BspEI T'CCGG_A Cuts at: 6726 6726 Size: 7063 BspHI T'CATG_A Cuts at: 4009 4162 4952 6682 4009 Size: 153 790 1730 4390 Fragments arranged by size: 4390 1730 790 153 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4067 4418 844 Size: 3223 351 3489 Fragments arranged by size: 3489 3223 351 BsrBI CCG'CTC Cuts at: 1394 1823 4950 1394 Size: 429 3127 3507 Fragments arranged by size: 3507 3127 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5558 5732 65 Size: 1183 4310 174 1396 Fragments arranged by size: 4310 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5707 5894 1700 Size: 110 3897 187 2869 Fragments arranged by size: 3897 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7063 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7063 BssSI C'ACGA_G Cuts at: 5123 6135 5123 Size: 1012 6051 BstAPI GCAn_nnn'nTGC Cuts at: 794 2384 794 Size: 1590 5473 BstBI TT'CG_AA Cuts at: 2975 5867 2975 Size: 2892 4171 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7063 BstXI CCAn_nnnn'nTGG Cuts at: 3775 3775 Size: 7063 BstYI r'GATC_y Cuts at: 1972 2793 3817 4169 4393 4760 5145 5162 6614 Size: 821 1024 352 224 367 385 17 1452 Cuts at: 6614 6700 6712 7058 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1024 821 385 367 352 346 224 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2547 2547 Size: 7063 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6511 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2946 487 Size: 683 56 1720 4604 Fragments arranged by size: 4604 1720 683 56 BtsI GCAGTG_nn' Cuts at: 4579 5373 5393 4579 Size: 794 20 6249 Fragments arranged by size: 6249 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7063 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3011 3046 529 Size: 35 2447 35 4546 Fragments arranged by size: 4546 2447 35 35 DraI TTT'AAA Cuts at: 3288 4367 3288 Size: 1079 5984 DraIII CAC_nnn'GTG Cuts at: 2587 2587 Size: 7063 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6070 809 Size: 507 4754 1802 Fragments arranged by size: 4754 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4254 4510 5399 Size: 54 1425 50 275 18 2424 256 889 Cuts at: 5399 7028 8 Size: 1629 43 Fragments arranged by size: 2424 1629 1425 889 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4510 1537 Size: 275 2698 4090 Fragments arranged by size: 4090 2698 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4064 4993 1353 Size: 2711 929 3423 Fragments arranged by size: 3423 2711 929 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5650 6024 6170 387 Size: 1064 138 4061 374 146 1280 Fragments arranged by size: 4061 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2091 2783 2854 3893 5126 5723 Size: 393 401 58 692 71 1039 1233 597 Cuts at: 5723 6510 6778 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1233 1039 787 692 597 401 393 268 71 58 EcoNI CCTnn'n_nnAGG Cuts at: 3704 3704 Size: 7063 EcoO109I rG'GnC_Cy Cuts at: 2551 2551 Size: 7063 EcoRI G'AATT_C Cuts at: 2606 2779 3226 4372 4750 2606 Size: 173 447 1146 378 4919 Fragments arranged by size: 4919 1146 447 378 173 EcoRV GAT'ATC Cuts at: 2260 2260 Size: 7063 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4102 4102 Size: 7063 FspI TGC'GCA Cuts at: 5569 5569 Size: 7063 HaeII r_GCGC'y Cuts at: 1678 6210 1678 Size: 4532 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2250 3184 3216 Size: 32 135 32 614 32 32 934 32 Cuts at: 3216 3481 3513 3594 3614 3626 3646 5704 5736 Size: 265 32 81 20 12 20 2058 32 Cuts at: 5736 5778 5810 1373 Size: 42 32 2626 Fragments arranged by size: 2626 2058 934 614 265 135 81 42 32 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 4407 4653 669 Size: 988 281 2469 246 3079 Fragments arranged by size: 3079 2469 988 281 246 HindIII A'AGCT_T Cuts at: 748 3408 748 Size: 2660 4403 HpaI GTT'AAC Cuts at: 4653 4653 Size: 7063 KpnI G_GTAC'C Cuts at: 4388 4388 Size: 7063 MfeI C'AATT_G Cuts at: 2478 3209 4662 2478 Size: 731 1453 4879 Fragments arranged by size: 4879 1453 731 MluI A'CGCG_T Cuts at: 6939 6939 Size: 7063 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3105 6177 6361 1305 Size: 1800 3072 184 2007 Fragments arranged by size: 3072 2007 1800 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4256 10 Size: 54 1425 343 2424 2817 Fragments arranged by size: 2817 2424 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3583 3776 5021 5380 5539 518 Size: 648 2417 193 1245 359 159 2042 Fragments arranged by size: 2417 2042 1245 648 359 193 159 MspA1I CmG'CkG Cuts at: 1606 2803 2863 4479 5156 6304 6549 1606 Size: 1197 60 1616 677 1148 245 2120 Fragments arranged by size: 2120 1616 1197 1148 677 245 60 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6511 NdeI CA'TA_TG Cuts at: 387 387 Size: 7063 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7063 NotI GC'GGCC_GC Cuts at: 4510 4510 Size: 7063 NsiI A_TGCA'T Cuts at: 2380 3922 2380 Size: 1542 5521 NspI r_CATG'y Cuts at: 1132 1229 3028 3721 4421 5966 1132 Size: 97 1799 693 700 1545 2229 Fragments arranged by size: 2229 1799 1545 700 693 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7063 PciI A'CATG_T Cuts at: 3717 5962 3717 Size: 2245 4818 PflMI CCAn_nnn'nTGG Cuts at: 1108 2287 1108 Size: 1179 5884 PfoI T'CCnGG_A Cuts at: 3454 3454 Size: 7063 PmeI GTTT'AAAC Cuts at: 4367 4367 Size: 7063 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5132 5164 6645 6677 5132 Size: 32 1481 32 5518 Fragments arranged by size: 5518 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7063 PsiI TTA'TAA Cuts at: 3666 4633 3666 Size: 967 6096 PstI C_TGCA'G Cuts at: 830 3809 4415 830 Size: 2979 606 3478 Fragments arranged by size: 3478 2979 606 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7063 PvuII CAG'CTG Cuts at: 2803 2863 2803 Size: 60 7003 SalI G'TCGA_C Cuts at: 1936 4405 1936 Size: 2469 4594 SbfI CC_TGCA'GG Cuts at: 4415 4415 Size: 7063 ScaI AGT'ACT Cuts at: 4214 5311 7023 4214 Size: 1097 1712 4254 Fragments arranged by size: 4254 1712 1097 SfcI C'TryA_G Cuts at: 826 1045 2640 2852 3805 4411 5546 6227 6418 Size: 219 1595 212 953 606 1135 681 191 Cuts at: 6418 826 Size: 1471 Fragments arranged by size: 1595 1471 1135 953 681 606 219 212 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7063 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7063 SmaI CCC'GGG Cuts at: 1872 4390 1872 Size: 2518 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 4318 5167 6068 6330 820 Size: 197 926 408 1967 849 901 262 1553 Fragments arranged by size: 1967 1553 926 901 849 408 262 197 SnaBI TAC'GTA Cuts at: 493 4854 493 Size: 4361 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7063 SphI G_CATG'C Cuts at: 1132 4421 1132 Size: 3289 3774 SspI AAT'ATT Cuts at: 5 52 4336 4987 5 Size: 47 4284 651 2081 Fragments arranged by size: 4284 2081 651 47 StuI AGG'CCT Cuts at: 1517 2906 1517 Size: 1389 5674 StyI C'CwwG_G Cuts at: 513 1065 4281 4502 513 Size: 552 3216 221 3074 Fragments arranged by size: 3216 3074 552 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3531 3943 5090 5107 5260 5445 5890 Size: 1255 1951 412 1147 17 153 185 445 Cuts at: 5890 325 Size: 1498 Fragments arranged by size: 1951 1498 1255 1147 445 412 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4212 5309 7021 Size: 274 80 33 51 1443 2235 1097 1712 Cuts at: 7021 96 Size: 138 Fragments arranged by size: 2235 1712 1443 1097 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3003 3035 7023 7055 3003 Size: 32 3988 32 3011 Fragments arranged by size: 3988 3011 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7063 XbaI T'CTAG_A Cuts at: 4399 4399 Size: 7063 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3778 3778 Size: 7063 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7063 XmaI C'CCGG_G Cuts at: 1870 4388 1870 Size: 2518 4545 XmnI GAAnn'nnTTC Cuts at: 2783 4203 5192 2783 Size: 1420 989 4654 Fragments arranged by size: 4654 1420 989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4871 276 Size: 53 83 186 936 3337 2468 Fragments arranged by size: 3337 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI