Restriction map for pFN21AA0012
 (Circular) (Six-base) MAPSORT of: pFN21AA0012.seq  Check: 8677  from: 1  to: 7063



pFN21AA0012  7063 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   4067    4067 
   Size:       7063
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4873     278 
   Size:         53      83     186     936    3337    2468

  Fragments arranged by size: 

                3337    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4406    1937 
   Size:       2469    4594
 
Acc65I G'GTAC_C

Cuts at:   4384    4384 
   Size:       7063
 
AclI AA'CG_TT

Cuts at:   1563    5190    5563    1563 
   Size:       3627     373    3063

  Fragments arranged by size: 

                3627    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2033    2091    2854    5126    6510    6778    1632 
   Size:        401      58     763    2272    1384     268    1917

  Fragments arranged by size: 

                2272    1917    1384     763     401     268      58
 
AflII C'TTAA_G

Cuts at:    820    1017    4318     820 
   Size:        197    3301    3565

  Fragments arranged by size: 

                3565    3301     197
 
AflIII A'CryG_T

Cuts at:   1203    3717    5962    6939    1203 
   Size:       2514    2245     977    1327

  Fragments arranged by size: 

                2514    2245    1327     977
 
AgeI A'CCGG_T

Cuts at:   5894    5894 
   Size:       7063
 
AhdI GACnn_n'nnGTC

Cuts at:   5792    5792 
   Size:       7063
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       7063
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2522    1184 
   Size:       1338    5725
 
AlwNI CAG_nnn'CTG

Cuts at:   3005    4806    6378    3005 
   Size:       1801    1572    3690

  Fragments arranged by size: 

                3690    1801    1572
 
ApaLI G'TGCA_C

Cuts at:   5120    6276    5120 
   Size:       1156    5907
 
ApoI r'AATT_y

Cuts at:   1493    2081    2273    2429    2606    2779    2992    3226    3605 
   Size:        588     192     156     177     173     213     234     379

Cuts at:   3605    4372    4598    4750    6859    1493 
   Size:        767     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     767     588     379     234     226     213
                 192     177     173     156     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4332    5617     160 
   Size:        872    3300    1285    1606

  Fragments arranged by size: 

                3300    1606    1285     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7063
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4388    1870 
   Size:         73    2445    4545

  Fragments arranged by size: 

                4545    2445      73
 
BamHI G'GATC_C

Cuts at:   2793    4393    4760    2793 
   Size:       1600     367    5096

  Fragments arranged by size: 

                5096    1600     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3111    4122    4384    5839     618 
   Size:        325     217     627    1324    1011     262    1455    1842

  Fragments arranged by size: 

                1842    1455    1324    1011     627     325     262     217
            
 
BanII G_rGCy'C

Cuts at:   1391    4382    1391 
   Size:       2991    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2547    3288     928 
   Size:        971     648     741    4703

  Fragments arranged by size: 

                4703     971     741     648
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    5254    5288    1903 
   Size:         34    3317      34    3678

  Fragments arranged by size: 

                3678    3317      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4955    6165    4955 
   Size:       1210    5853
 
BclI T'GATC_A

Cuts at:   1548    3489    3496    1548 
   Size:       1941       7    5115

  Fragments arranged by size: 

                5115    1941       7
 
BfrBI ATG'CAT

Cuts at:   2378    3920    2378 
   Size:       1542    5521
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5674     136 
   Size:        107     122      71     631     605    4002    1525

  Fragments arranged by size: 

                4002    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   7058    7058 
   Size:       7063
 
BlpI GC'TnA_GC

Cuts at:   4480    4480 
   Size:       7063
 
Bme1580I G_kGCm'C

Cuts at:   1790    3114    3942    5124    6280    1790 
   Size:       1324     828    1182    1156    2573

  Fragments arranged by size: 

                2573    1324    1182    1156     828
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       7063
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4794    5752     442 
   Size:        416      53      83     399      24    3377     958    1753

  Fragments arranged by size: 

                3377    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       7063
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2783    3893    5723    1239 
   Size:       1544    1110    1830    2579

  Fragments arranged by size: 

                2579    1830    1544    1110
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2336    5188    6053    6351    2336 
   Size:       2852     865     298    3048

  Fragments arranged by size: 

                3048    2852     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2114    5726     882 
   Size:        617     615    3612    2219

  Fragments arranged by size: 

                3612    2219     617     615
 
BsaAI yAC'GTr

Cuts at:    493    1204    4854     493 
   Size:        711    3650    2702

  Fragments arranged by size: 

                3650    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   7057    7057 
   Size:       7063
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4870    5252    5887     275 
   Size:         53      83     186     936    3337     382     635    1451

  Fragments arranged by size: 

                3337    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   5496    5894    6168    6315    6726    5496 
   Size:        398     274     147     411    5833

  Fragments arranged by size: 

                5833     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3594    3624    1178 
   Size:         30     165      30    2191      30    4617

  Fragments arranged by size: 

                4617    2191     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       7063
 
BseYI C'CCAG_C

Cuts at:   6266    6762    6266 
   Size:        496    6567
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2564    1163 
   Size:        573     828    5662

  Fragments arranged by size: 

                5662     828     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4513    5274    6302    1540 
   Size:        275     186    2512     761    1028    2301

  Fragments arranged by size: 

                2512    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2447    5124    5209    6280    2447 
   Size:       2677      85    1071    3230

  Fragments arranged by size: 

                3230    2677    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3381    4574    4667    3381 
   Size:       1193      93    5777

  Fragments arranged by size: 

                5777    1193      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2447    3114    3942    4382    5124    5209    6280 
   Size:        399     657     667     828     440     742      85    1071

Cuts at:   6280    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     828     742     667     657     440     399
                  85
 
BspEI T'CCGG_A

Cuts at:   6726    6726 
   Size:       7063
 
BspHI T'CATG_A

Cuts at:   4009    4162    4952    6682    4009 
   Size:        153     790    1730    4390

  Fragments arranged by size: 

                4390    1730     790     153
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    4067    4418     844 
   Size:       3223     351    3489

  Fragments arranged by size: 

                3489    3223     351
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4950    1394 
   Size:        429    3127    3507

  Fragments arranged by size: 

                3507    3127     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    5558    5732      65 
   Size:       1183    4310     174    1396

  Fragments arranged by size: 

                4310    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5707    5894    1700 
   Size:        110    3897     187    2869

  Fragments arranged by size: 

                3897    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       7063
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7063
 
BssSI C'ACGA_G

Cuts at:   5123    6135    5123 
   Size:       1012    6051
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2384     794 
   Size:       1590    5473
 
BstBI TT'CG_AA

Cuts at:   2975    5867    2975 
   Size:       2892    4171
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       7063
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3775    3775 
   Size:       7063
 
BstYI r'GATC_y

Cuts at:   1972    2793    3817    4169    4393    4760    5145    5162    6614 
   Size:        821    1024     352     224     367     385      17    1452

Cuts at:   6614    6700    6712    7058    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1024     821     385     367     352     346
                 224      86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2547    2547 
   Size:       7063
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    6511
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2946     487 
   Size:        683      56    1720    4604

  Fragments arranged by size: 

                4604    1720     683      56
 
BtsI GCAGTG_nn'

Cuts at:   4579    5373    5393    4579 
   Size:        794      20    6249

  Fragments arranged by size: 

                6249     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7063
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    3011    3046     529 
   Size:         35    2447      35    4546

  Fragments arranged by size: 

                4546    2447      35      35
 
DraI TTT'AAA

Cuts at:   3288    4367    3288 
   Size:       1079    5984
 
DraIII CAC_nnn'GTG

Cuts at:   2587    2587 
   Size:       7063
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    6070     809 
   Size:        507    4754    1802

  Fragments arranged by size: 

                4754    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    4254    4510    5399 
   Size:         54    1425      50     275      18    2424     256     889

Cuts at:   5399    7028       8 
   Size:       1629      43

  Fragments arranged by size: 

                2424    1629    1425     889     275     256      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4510    1537 
   Size:        275    2698    4090

  Fragments arranged by size: 

                4090    2698     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    4064    4993    1353 
   Size:       2711     929    3423

  Fragments arranged by size: 

                3423    2711     929
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5650    6024    6170     387 
   Size:       1064     138    4061     374     146    1280

  Fragments arranged by size: 

                4061    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2033    2091    2783    2854    3893    5126    5723 
   Size:        393     401      58     692      71    1039    1233     597

Cuts at:   5723    6510    6778    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524    1233    1039     787     692     597     401     393
                 268      71      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3704    3704 
   Size:       7063
 
EcoO109I rG'GnC_Cy

Cuts at:   2551    2551 
   Size:       7063
 
EcoRI G'AATT_C

Cuts at:   2606    2779    3226    4372    4750    2606 
   Size:        173     447    1146     378    4919

  Fragments arranged by size: 

                4919    1146     447     378     173
 
EcoRV GAT'ATC

Cuts at:   2260    2260 
   Size:       7063
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4102    4102 
   Size:       7063
 
FspI TGC'GCA

Cuts at:   5569    5569 
   Size:       7063
 
HaeII r_GCGC'y

Cuts at:   1678    6210    1678 
   Size:       4532    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2186    2218    2250    3184    3216 
   Size:         32     135      32     614      32      32     934      32

Cuts at:   3216    3481    3513    3594    3614    3626    3646    5704    5736 
   Size:        265      32      81      20      12      20    2058      32

Cuts at:   5736    5778    5810    1373 
   Size:         42      32    2626

  Fragments arranged by size: 

                2626    2058     934     614     265     135      81      42
                  32      32      32      32      32      32      32      32
                  20      20      12
 
HincII GTy'rAC

Cuts at:    669    1657    1938    4407    4653     669 
   Size:        988     281    2469     246    3079

  Fragments arranged by size: 

                3079    2469     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3408     748 
   Size:       2660    4403
 
HpaI GTT'AAC

Cuts at:   4653    4653 
   Size:       7063
 
KpnI G_GTAC'C

Cuts at:   4388    4388 
   Size:       7063
 
MfeI C'AATT_G

Cuts at:   2478    3209    4662    2478 
   Size:        731    1453    4879

  Fragments arranged by size: 

                4879    1453     731
 
MluI A'CGCG_T

Cuts at:   6939    6939 
   Size:       7063
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3105    6177    6361    1305 
   Size:       1800    3072     184    2007

  Fragments arranged by size: 

                3072    2007    1800     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    4256      10 
   Size:         54    1425     343    2424    2817

  Fragments arranged by size: 

                2817    2424    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3583    3776    5021    5380    5539     518 
   Size:        648    2417     193    1245     359     159    2042

  Fragments arranged by size: 

                2417    2042    1245     648     359     193     159
 
MspA1I CmG'CkG

Cuts at:   1606    2803    2863    4479    5156    6304    6549    1606 
   Size:       1197      60    1616     677    1148     245    2120

  Fragments arranged by size: 

                2120    1616    1197    1148     677     245      60
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    6511
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       7063
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       7063
 
NotI GC'GGCC_GC

Cuts at:   4510    4510 
   Size:       7063
 
NsiI A_TGCA'T

Cuts at:   2380    3922    2380 
   Size:       1542    5521
 
NspI r_CATG'y

Cuts at:   1132    1229    3028    3721    4421    5966    1132 
   Size:         97    1799     693     700    1545    2229

  Fragments arranged by size: 

                2229    1799    1545     700     693      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       7063
 
PciI A'CATG_T

Cuts at:   3717    5962    3717 
   Size:       2245    4818
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2287    1108 
   Size:       1179    5884
 
PfoI T'CCnGG_A

Cuts at:   3454    3454 
   Size:       7063
 
PmeI GTTT'AAAC

Cuts at:   4367    4367 
   Size:       7063
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5132    5164    6645    6677    5132 
   Size:         32    1481      32    5518

  Fragments arranged by size: 

                5518    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       7063
 
PsiI TTA'TAA

Cuts at:   3666    4633    3666 
   Size:        967    6096
 
PstI C_TGCA'G

Cuts at:    830    3809    4415     830 
   Size:       2979     606    3478

  Fragments arranged by size: 

                3478    2979     606
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7063
 
PvuII CAG'CTG

Cuts at:   2803    2863    2803 
   Size:         60    7003
 
SalI G'TCGA_C

Cuts at:   1936    4405    1936 
   Size:       2469    4594
 
SbfI CC_TGCA'GG

Cuts at:   4415    4415 
   Size:       7063
 
ScaI AGT'ACT

Cuts at:   4214    5311    7023    4214 
   Size:       1097    1712    4254

  Fragments arranged by size: 

                4254    1712    1097
 
SfcI C'TryA_G

Cuts at:    826    1045    2640    2852    3805    4411    5546    6227    6418 
   Size:        219    1595     212     953     606    1135     681     191

Cuts at:   6418     826 
   Size:       1471

  Fragments arranged by size: 

                1595    1471    1135     953     681     606     219     212
                 191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7063
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7063
 
SmaI CCC'GGG

Cuts at:   1872    4390    1872 
   Size:       2518    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2351    4318    5167    6068    6330     820 
   Size:        197     926     408    1967     849     901     262    1553

  Fragments arranged by size: 

                1967    1553     926     901     849     408     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    4854     493 
   Size:       4361    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7063
 
SphI G_CATG'C

Cuts at:   1132    4421    1132 
   Size:       3289    3774
 
SspI AAT'ATT

Cuts at:      5      52    4336    4987       5 
   Size:         47    4284     651    2081

  Fragments arranged by size: 

                4284    2081     651      47
 
StuI AGG'CCT

Cuts at:   1517    2906    1517 
   Size:       1389    5674
 
StyI C'CwwG_G

Cuts at:    513    1065    4281    4502     513 
   Size:        552    3216     221    3074

  Fragments arranged by size: 

                3216    3074     552     221
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3531    3943    5090    5107    5260    5445    5890 
   Size:       1255    1951     412    1147      17     153     185     445

Cuts at:   5890     325 
   Size:       1498

  Fragments arranged by size: 

                1951    1498    1255    1147     445     412     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4212    5309    7021 
   Size:        274      80      33      51    1443    2235    1097    1712

Cuts at:   7021      96 
   Size:        138

  Fragments arranged by size: 

                2235    1712    1443    1097     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3003    3035    7023    7055    3003 
   Size:         32    3988      32    3011

  Fragments arranged by size: 

                3988    3011      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       7063
 
XbaI T'CTAG_A

Cuts at:   4399    4399 
   Size:       7063
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3778    3778 
   Size:       7063
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7063
 
XmaI C'CCGG_G

Cuts at:   1870    4388    1870 
   Size:       2518    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2783    4203    5192    2783 
   Size:       1420     989    4654

  Fragments arranged by size: 

                4654    1420     989
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4871     276 
   Size:         53      83     186     936    3337    2468

  Fragments arranged by size: 

                3337    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I  EcoICRI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     PacI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI
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