(Circular) (Six-base) MAPSORT of: pFN21AA0003.seq Check: 3603 from: 1 to: 6199 pFN21AA0003 6199 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4009 278 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3542 1937 Size: 1605 4594 Acc65I G'GTAC_C Cuts at: 3520 3520 Size: 6199 AclI AA'CG_TT Cuts at: 1563 4326 4699 1563 Size: 2763 373 3063 Fragments arranged by size: 3063 2763 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2729 4262 5646 5914 1632 Size: 1097 1533 1384 268 1917 Fragments arranged by size: 1917 1533 1384 1097 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6002 AflIII A'CryG_T Cuts at: 1203 2221 2266 5098 6075 1203 Size: 1018 45 2832 977 1327 Fragments arranged by size: 2832 1327 1018 977 45 AgeI A'CCGG_T Cuts at: 5030 5030 Size: 6199 AhdI GACnn_n'nnGTC Cuts at: 4928 4928 Size: 6199 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6199 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2229 1184 Size: 1045 5154 AlwNI CAG_nnn'CTG Cuts at: 2293 2494 2701 2758 3942 5514 2293 Size: 201 207 57 1184 1572 2978 Fragments arranged by size: 2978 1572 1184 207 201 57 ApaI G_GGCC'C Cuts at: 3450 3450 Size: 6199 ApaLI G'TGCA_C Cuts at: 4256 5412 4256 Size: 1156 5043 ApoI r'AATT_y Cuts at: 1493 2500 3508 3734 3886 5995 1493 Size: 1007 1008 226 152 2109 1697 Fragments arranged by size: 2109 1697 1008 1007 226 152 AseI AT'TA_AT Cuts at: 160 1032 2916 4753 160 Size: 872 1884 1837 1606 Fragments arranged by size: 1884 1837 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 AvaI C'yCGr_G Cuts at: 1870 1943 3524 1870 Size: 73 1581 4545 Fragments arranged by size: 4545 1581 73 BamHI G'GATC_C Cuts at: 3529 3896 3529 Size: 367 5832 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3520 4975 618 Size: 325 217 627 1733 1455 1842 Fragments arranged by size: 1842 1733 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3450 3518 1391 Size: 2059 68 4072 Fragments arranged by size: 4072 2059 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5228 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4390 4424 1903 Size: 34 2453 34 3678 Fragments arranged by size: 3678 2453 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4091 5301 4091 Size: 1210 4989 BclI T'GATC_A Cuts at: 1548 1548 Size: 6199 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4810 136 Size: 107 122 71 631 605 3138 1525 Fragments arranged by size: 3138 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6194 6194 Size: 6199 BlpI GC'TnA_GC Cuts at: 3616 3616 Size: 6199 Bme1580I G_kGCm'C Cuts at: 1790 3325 3450 4260 5416 1790 Size: 1535 125 810 1156 2573 Fragments arranged by size: 2573 1535 1156 810 125 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6199 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3151 3240 3444 Size: 416 53 83 399 24 1734 89 204 Cuts at: 3444 3930 4888 442 Size: 486 958 1753 Fragments arranged by size: 1753 1734 958 486 416 399 204 89 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6199 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2232 2232 Size: 6199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 2516 2780 4859 1239 Size: 1169 108 264 2079 2579 Fragments arranged by size: 2579 2079 1169 264 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2324 2501 4324 5189 5487 2324 Size: 177 1823 865 298 3036 Fragments arranged by size: 3036 1823 865 298 177 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2444 4862 882 Size: 617 945 2418 2219 Fragments arranged by size: 2418 2219 945 617 BsaAI yAC'GTr Cuts at: 493 1204 2224 3990 493 Size: 711 1020 1766 2702 Fragments arranged by size: 2702 1766 1020 711 BsaBI GATnn'nnATC Cuts at: 2871 6193 2871 Size: 3322 2877 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4006 4388 5023 275 Size: 53 83 186 936 2473 382 635 1451 Fragments arranged by size: 2473 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2106 2230 3059 4632 5030 5304 5451 5862 2106 Size: 124 829 1573 398 274 147 411 2443 Fragments arranged by size: 2443 1573 829 411 398 274 147 124 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5974 Fragments arranged by size: 5974 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 1186 Size: 1210 4989 BseYI C'CCAG_C Cuts at: 3073 5402 5898 3073 Size: 2329 496 3374 Fragments arranged by size: 3374 2329 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2080 2883 1163 Size: 573 344 803 4479 Fragments arranged by size: 4479 803 573 344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3649 4410 5438 1540 Size: 275 186 1648 761 1028 2301 Fragments arranged by size: 2301 1648 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4260 4345 5416 4260 Size: 85 1071 5043 Fragments arranged by size: 5043 1071 85 BsmI GAATG_Cn' Cuts at: 2362 3710 3803 2362 Size: 1348 93 4758 Fragments arranged by size: 4758 1348 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3325 3450 3518 4260 4345 5416 1391 Size: 399 1535 125 68 742 85 1071 2174 Fragments arranged by size: 2174 1535 1071 742 399 125 85 68 BspEI T'CCGG_A Cuts at: 5862 5862 Size: 6199 BspHI T'CATG_A Cuts at: 4088 5818 4088 Size: 1730 4469 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3554 844 Size: 2710 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4086 1394 Size: 429 2263 3507 Fragments arranged by size: 3507 2263 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2129 4694 4868 65 Size: 1183 881 2565 174 1396 Fragments arranged by size: 2565 1396 1183 881 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4843 5030 1700 Size: 110 3033 187 2869 Fragments arranged by size: 3033 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6199 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6199 BssSI C'ACGA_G Cuts at: 4259 5271 4259 Size: 1012 5187 BstAPI GCAn_nnn'nTGC Cuts at: 794 3118 794 Size: 2324 3875 BstBI TT'CG_AA Cuts at: 5003 5003 Size: 6199 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6199 BstYI r'GATC_y Cuts at: 1972 2562 3529 3896 4281 4298 5750 5836 5848 Size: 590 967 367 385 17 1452 86 12 Cuts at: 5848 6194 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 967 590 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3406 513 Size: 552 2341 3306 Fragments arranged by size: 3306 2341 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5460 Fragments arranged by size: 5460 683 56 BtsI GCAGTG_nn' Cuts at: 3715 4509 4529 3715 Size: 794 20 5385 Fragments arranged by size: 5385 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6199 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6164 DraI TTT'AAA Cuts at: 3219 3503 3219 Size: 284 5915 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5206 809 Size: 507 3890 1802 Fragments arranged by size: 3890 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3646 4535 6164 Size: 54 1425 50 275 18 1816 889 1629 Cuts at: 6164 8 Size: 43 Fragments arranged by size: 1816 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3646 1537 Size: 275 1834 4090 Fragments arranged by size: 4090 1834 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2245 2619 2640 2835 4129 1353 Size: 892 374 21 195 1294 3423 Fragments arranged by size: 3423 1294 892 374 195 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4786 5160 5306 387 Size: 1064 138 3197 374 146 1280 Fragments arranged by size: 3197 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 2516 2729 2780 4262 4859 5646 Size: 393 776 108 213 51 1482 597 787 Cuts at: 5646 5914 1239 Size: 268 1524 Fragments arranged by size: 1524 1482 787 776 597 393 268 213 108 51 EcoO109I rG'GnC_Cy Cuts at: 3446 3446 Size: 6199 EcoRI G'AATT_C Cuts at: 2500 3508 3886 2500 Size: 1008 378 4813 Fragments arranged by size: 4813 1008 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2675 2675 Size: 6199 FspI TGC'GCA Cuts at: 4705 4705 Size: 6199 HaeII r_GCGC'y Cuts at: 1678 2074 5346 1678 Size: 396 3272 2531 Fragments arranged by size: 3272 2531 396 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4840 4872 4914 4946 1373 Size: 32 135 32 3268 32 42 32 2626 Fragments arranged by size: 3268 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3333 3543 3789 669 Size: 988 281 1395 210 246 3079 Fragments arranged by size: 3079 1395 988 281 246 210 HindIII A'AGCT_T Cuts at: 748 748 Size: 6199 HpaI GTT'AAC Cuts at: 3333 3789 3333 Size: 456 5743 KpnI G_GTAC'C Cuts at: 3524 3524 Size: 6199 MfeI C'AATT_G Cuts at: 3798 3798 Size: 6199 MluI A'CGCG_T Cuts at: 6075 6075 Size: 6199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 2610 2954 5313 5497 1305 Size: 1008 297 344 2359 184 2007 Fragments arranged by size: 2359 2007 1008 344 297 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4377 Fragments arranged by size: 4377 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3473 4157 4516 4675 518 Size: 648 2307 684 359 159 2042 Fragments arranged by size: 2307 2042 684 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2491 3615 4292 5440 5685 1606 Size: 885 1124 677 1148 245 2120 Fragments arranged by size: 2120 1148 1124 885 677 245 NcoI C'CATG_G Cuts at: 513 1065 3406 513 Size: 552 2341 3306 Fragments arranged by size: 3306 2341 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6199 NotI GC'GGCC_GC Cuts at: 3646 3646 Size: 6199 NspI r_CATG'y Cuts at: 1132 1229 2270 3121 3557 5102 1132 Size: 97 1041 851 436 1545 2229 Fragments arranged by size: 2229 1545 1041 851 436 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6199 PciI A'CATG_T Cuts at: 2266 5098 2266 Size: 2832 3367 PflMI CCAn_nnn'nTGG Cuts at: 1108 2701 1108 Size: 1593 4606 PfoI T'CCnGG_A Cuts at: 2694 2694 Size: 6199 PmeI GTTT'AAAC Cuts at: 3503 3503 Size: 6199 PmlI CAC'GTG Cuts at: 2224 2224 Size: 6199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4268 4300 5781 5813 4268 Size: 32 1481 32 4654 Fragments arranged by size: 4654 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6199 PsiI TTA'TAA Cuts at: 3769 3769 Size: 6199 PspOMI G'GGCC_C Cuts at: 3446 3446 Size: 6199 PstI C_TGCA'G Cuts at: 830 2208 2422 3551 830 Size: 1378 214 1129 3478 Fragments arranged by size: 3478 1378 1129 214 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6199 PvuII CAG'CTG Cuts at: 2491 2491 Size: 6199 SalI G'TCGA_C Cuts at: 1936 3541 1936 Size: 1605 4594 SbfI CC_TGCA'GG Cuts at: 3551 3551 Size: 6199 ScaI AGT'ACT Cuts at: 4447 6159 4447 Size: 1712 4487 SfcI C'TryA_G Cuts at: 826 1045 2204 2418 3205 3547 4682 5363 5554 Size: 219 1159 214 787 342 1135 681 191 Cuts at: 5554 826 Size: 1471 Fragments arranged by size: 1471 1159 1135 787 681 342 219 214 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6199 SmaI CCC'GGG Cuts at: 1872 3526 1872 Size: 1654 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2303 2480 4303 5204 5466 820 Size: 197 926 360 177 1823 901 262 1553 Fragments arranged by size: 1823 1553 926 901 360 262 197 177 SnaBI TAC'GTA Cuts at: 493 3990 493 Size: 3497 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6199 SphI G_CATG'C Cuts at: 1132 3557 1132 Size: 2425 3774 SspI AAT'ATT Cuts at: 5 52 3068 4123 5 Size: 47 3016 1055 2081 Fragments arranged by size: 3016 2081 1055 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6199 StyI C'CwwG_G Cuts at: 513 1065 3406 3638 513 Size: 552 2341 232 3074 Fragments arranged by size: 3074 2341 552 232 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4226 4243 4396 4581 5026 325 Size: 1255 2646 17 153 185 445 1498 Fragments arranged by size: 2646 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 3114 4445 Size: 274 80 33 51 1443 210 927 1331 Cuts at: 4445 6157 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1331 927 274 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6159 6191 6159 Size: 32 6167 Tth111I GACn'n_nGTC Cuts at: 1316 2774 1316 Size: 1458 4741 XbaI T'CTAG_A Cuts at: 3007 3535 3007 Size: 528 5671 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6199 XmaI C'CCGG_G Cuts at: 1870 3524 1870 Size: 1654 4545 XmnI GAAnn'nnTTC Cuts at: 4328 4328 Size: 6199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4007 276 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI