(Circular) (Six-base) MAPSORT of: pFN21AB7890.seq Check: 1821 from: 1 to: 6661 pFN21AB7890 6661 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4471 278 Size: 53 83 186 936 2935 2468 Fragments arranged by size: 2935 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4004 1937 Size: 2067 4594 Acc65I G'GTAC_C Cuts at: 3982 3982 Size: 6661 AclI AA'CG_TT Cuts at: 1563 3694 4788 5161 1563 Size: 2131 1094 373 3063 Fragments arranged by size: 3063 2131 1094 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2744 3122 3477 3636 3933 4724 6108 6376 Size: 1112 378 355 159 297 791 1384 268 Cuts at: 6376 1632 Size: 1917 Fragments arranged by size: 1917 1384 1112 791 378 355 297 268 159 AfeI AGC'GCT Cuts at: 3567 3567 Size: 6661 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6464 AflIII A'CryG_T Cuts at: 1203 5560 6537 1203 Size: 4357 977 1327 Fragments arranged by size: 4357 1327 977 AgeI A'CCGG_T Cuts at: 5492 5492 Size: 6661 AhdI GACnn_n'nnGTC Cuts at: 2575 5390 2575 Size: 2815 3846 AleI CACnn'nnGTG Cuts at: 1166 3197 1166 Size: 2031 4630 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6661 AlwNI CAG_nnn'CTG Cuts at: 2722 4404 5976 2722 Size: 1682 1572 3407 Fragments arranged by size: 3407 1682 1572 ApaI G_GGCC'C Cuts at: 3222 3222 Size: 6661 ApaLI G'TGCA_C Cuts at: 4718 5874 4718 Size: 1156 5505 ApoI r'AATT_y Cuts at: 1493 2694 2712 3020 3583 3970 4196 4348 6457 Size: 1201 18 308 563 387 226 152 2109 Cuts at: 6457 1493 Size: 1697 Fragments arranged by size: 2109 1697 1201 563 387 308 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 5215 160 Size: 872 4183 1606 Fragments arranged by size: 4183 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 AvaI C'yCGr_G Cuts at: 1870 1943 2128 3986 1870 Size: 73 185 1858 4545 Fragments arranged by size: 4545 1858 185 73 BamHI G'GATC_C Cuts at: 3076 3991 4358 3076 Size: 915 367 5379 Fragments arranged by size: 5379 915 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3982 5437 618 Size: 325 217 627 2195 1455 1842 Fragments arranged by size: 2195 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3222 3980 1391 Size: 1831 758 4072 Fragments arranged by size: 4072 1831 758 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2160 3139 3315 3912 928 Size: 971 261 979 176 597 3677 Fragments arranged by size: 3677 979 971 597 261 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2744 2778 4852 4886 1903 Size: 34 807 34 2074 34 3678 Fragments arranged by size: 3678 2074 807 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4553 5763 4553 Size: 1210 5451 BclI T'GATC_A Cuts at: 1548 2514 1548 Size: 966 5695 BfrBI ATG'CAT Cuts at: 3757 3757 Size: 6661 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3529 5272 136 Size: 107 122 71 631 605 1857 1743 1525 Fragments arranged by size: 1857 1743 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2314 6656 2314 Size: 4342 2319 BlpI GC'TnA_GC Cuts at: 4078 4078 Size: 6661 Bme1580I G_kGCm'C Cuts at: 1790 2136 3222 4722 5878 1790 Size: 346 1086 1500 1156 2573 Fragments arranged by size: 2573 1500 1156 1086 346 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6661 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3125 4392 5350 Size: 416 53 83 399 24 1708 1267 958 Cuts at: 5350 442 Size: 1753 Fragments arranged by size: 1753 1708 1267 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6661 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2125 2303 2862 3000 3165 3180 3526 3659 Size: 886 178 559 138 165 15 346 133 Cuts at: 3659 3795 5321 1239 Size: 136 1526 2579 Fragments arranged by size: 2579 1526 886 559 346 178 165 138 136 133 15 Bpu10I CC'TnA_GC Cuts at: 3665 3665 Size: 6661 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3534 4786 5651 5949 3534 Size: 1252 865 298 4246 Fragments arranged by size: 4246 1252 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2280 2639 3644 5324 882 Size: 617 781 359 1005 1680 2219 Fragments arranged by size: 2219 1680 1005 781 617 359 BsaAI yAC'GTr Cuts at: 493 1204 3357 3831 4452 493 Size: 711 2153 474 621 2702 Fragments arranged by size: 2702 2153 711 621 474 BsaBI GATnn'nnATC Cuts at: 6655 6655 Size: 6661 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4468 4850 5485 275 Size: 53 83 186 936 2935 382 635 1451 Fragments arranged by size: 2935 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5094 5492 5766 5913 6324 5094 Size: 398 274 147 411 5431 Fragments arranged by size: 5431 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6436 Fragments arranged by size: 6436 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 5781 BseYI C'CCAG_C Cuts at: 2873 5864 6360 2873 Size: 2991 496 3174 Fragments arranged by size: 3174 2991 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6088 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4111 4872 5900 1540 Size: 275 186 2110 761 1028 2301 Fragments arranged by size: 2301 2110 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3704 4722 4807 5878 3704 Size: 1018 85 1071 4487 Fragments arranged by size: 4487 1071 1018 85 BsmI GAATG_Cn' Cuts at: 3418 4172 4265 3418 Size: 754 93 5814 Fragments arranged by size: 5814 754 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 3222 3704 3980 4722 4807 5878 Size: 399 346 1086 482 276 742 85 1071 Cuts at: 5878 1391 Size: 2174 Fragments arranged by size: 2174 1086 1071 742 482 399 346 276 85 BspEI T'CCGG_A Cuts at: 6324 6324 Size: 6661 BspHI T'CATG_A Cuts at: 2167 4550 6280 2167 Size: 2383 1730 2548 Fragments arranged by size: 2548 2383 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4016 844 Size: 3172 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4548 1394 Size: 429 2725 3507 Fragments arranged by size: 3507 2725 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3449 3855 5156 5330 65 Size: 1183 2201 406 1301 174 1396 Fragments arranged by size: 2201 1396 1301 1183 406 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5305 5492 1700 Size: 110 3495 187 2869 Fragments arranged by size: 3495 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6661 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6661 BssSI C'ACGA_G Cuts at: 4721 5733 4721 Size: 1012 5649 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6661 BstBI TT'CG_AA Cuts at: 2151 3841 5465 2151 Size: 1690 1624 3347 Fragments arranged by size: 3347 1690 1624 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6661 BstYI r'GATC_y Cuts at: 1972 2314 2455 3076 3991 4358 4743 4760 6212 Size: 342 141 621 915 367 385 17 1452 Cuts at: 6212 6298 6310 6656 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 915 621 385 367 346 342 141 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2118 513 Size: 552 939 114 5056 Fragments arranged by size: 5056 939 552 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5922 Fragments arranged by size: 5922 683 56 BtsI GCAGTG_nn' Cuts at: 4177 4971 4991 4177 Size: 794 20 5847 Fragments arranged by size: 5847 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6661 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6626 DraI TTT'AAA Cuts at: 2923 3965 2923 Size: 1042 5619 DraIII CAC_nnn'GTG Cuts at: 2798 2798 Size: 6661 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5668 809 Size: 507 4352 1802 Fragments arranged by size: 4352 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3351 4108 4997 Size: 54 1425 50 275 18 1521 757 889 Cuts at: 4997 6626 8 Size: 1629 43 Fragments arranged by size: 1629 1521 1425 889 757 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4108 1537 Size: 275 2296 4090 Fragments arranged by size: 4090 2296 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 2248 4591 1353 Size: 800 95 2343 3423 Fragments arranged by size: 3423 2343 800 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5248 5622 5768 387 Size: 1064 138 3659 374 146 1280 Fragments arranged by size: 3659 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2125 2303 2744 2862 3000 3122 3165 Size: 393 493 178 441 118 138 122 43 Cuts at: 3165 3180 3477 3526 3636 3659 3795 3933 4724 Size: 15 297 49 110 23 136 138 791 Cuts at: 4724 5321 6108 6376 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 791 787 597 493 441 393 297 268 178 138 138 136 122 118 110 49 43 23 15 EcoNI CCTnn'n_nnAGG Cuts at: 2048 2048 Size: 6661 EcoO109I rG'GnC_Cy Cuts at: 3219 3426 3737 3219 Size: 207 311 6143 Fragments arranged by size: 6143 311 207 EcoRI G'AATT_C Cuts at: 2712 3970 4348 2712 Size: 1258 378 5025 Fragments arranged by size: 5025 1258 378 EcoRV GAT'ATC Cuts at: 3820 3820 Size: 6661 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2617 2924 2617 Size: 307 6354 FspI TGC'GCA Cuts at: 5167 5167 Size: 6661 HaeII r_GCGC'y Cuts at: 1678 3569 5808 1678 Size: 1891 2239 2531 Fragments arranged by size: 2531 2239 1891 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2440 2472 2561 2593 3766 Size: 32 135 32 868 32 89 32 1173 Cuts at: 3766 3798 5302 5334 5376 5408 1373 Size: 32 1504 32 42 32 2626 Fragments arranged by size: 2626 1504 1173 868 135 89 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4005 4251 669 Size: 988 281 2067 246 3079 Fragments arranged by size: 3079 2067 988 281 246 HindIII A'AGCT_T Cuts at: 748 2418 748 Size: 1670 4991 HpaI GTT'AAC Cuts at: 4251 4251 Size: 6661 KpnI G_GTAC'C Cuts at: 3986 3986 Size: 6661 MfeI C'AATT_G Cuts at: 2441 4260 2441 Size: 1819 4842 MluI A'CGCG_T Cuts at: 6537 6537 Size: 6661 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2013 5775 5959 1305 Size: 708 3762 184 2007 Fragments arranged by size: 3762 2007 708 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3353 10 Size: 54 1425 343 1521 3318 Fragments arranged by size: 3318 1521 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3197 4619 4978 5137 518 Size: 648 2031 1422 359 159 2042 Fragments arranged by size: 2042 2031 1422 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4077 4754 5902 6147 1606 Size: 2471 677 1148 245 2120 Fragments arranged by size: 2471 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5170 Fragments arranged by size: 5170 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6661 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6661 NotI GC'GGCC_GC Cuts at: 4108 4108 Size: 6661 NsiI A_TGCA'T Cuts at: 3759 3759 Size: 6661 NspI r_CATG'y Cuts at: 1132 1229 4019 5564 1132 Size: 97 2790 1545 2229 Fragments arranged by size: 2790 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6661 PciI A'CATG_T Cuts at: 5560 5560 Size: 6661 PflMI CCAn_nnn'nTGG Cuts at: 1108 3073 1108 Size: 1965 4696 PfoI T'CCnGG_A Cuts at: 3422 3675 3422 Size: 253 6408 PmeI GTTT'AAAC Cuts at: 3965 3965 Size: 6661 PmlI CAC'GTG Cuts at: 3357 3357 Size: 6661 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4730 4762 6243 6275 4730 Size: 32 1481 32 5116 Fragments arranged by size: 5116 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3426 3737 3426 Size: 311 6350 PshAI GACnn'nnGTC Cuts at: 1613 3139 1613 Size: 1526 5135 PsiI TTA'TAA Cuts at: 2658 4231 2658 Size: 1573 5088 PspOMI G'GGCC_C Cuts at: 3218 3218 Size: 6661 PstI C_TGCA'G Cuts at: 830 4013 830 Size: 3183 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6661 SalI G'TCGA_C Cuts at: 1936 4003 1936 Size: 2067 4594 SanDI GG'GwC_CC Cuts at: 3737 3737 Size: 6661 SbfI CC_TGCA'GG Cuts at: 4013 4013 Size: 6661 ScaI AGT'ACT Cuts at: 3547 4909 6621 3547 Size: 1362 1712 3587 Fragments arranged by size: 3587 1712 1362 SexAI A'CCwGG_T Cuts at: 3949 3949 Size: 6661 SfcI C'TryA_G Cuts at: 826 1045 3223 4009 5144 5825 6016 826 Size: 219 2178 786 1135 681 191 1471 Fragments arranged by size: 2178 1471 1135 786 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6661 SmaI CCC'GGG Cuts at: 1872 3988 1872 Size: 2116 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3549 4765 5666 5928 820 Size: 197 926 1606 1216 901 262 1553 Fragments arranged by size: 1606 1553 1216 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4452 493 Size: 3959 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6661 SphI G_CATG'C Cuts at: 1132 4019 1132 Size: 2887 3774 SspI AAT'ATT Cuts at: 5 52 3630 4585 5 Size: 47 3578 955 2081 Fragments arranged by size: 3578 2081 955 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6661 StyI C'CwwG_G Cuts at: 513 1065 2004 4100 513 Size: 552 939 2096 3074 Fragments arranged by size: 3074 2096 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2541 4688 4705 4858 5043 5488 325 Size: 1255 961 2147 17 153 185 445 1498 Fragments arranged by size: 2147 1498 1255 961 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2615 3545 4907 Size: 274 80 33 51 1443 638 930 1362 Cuts at: 4907 6619 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1362 930 638 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6621 6653 6621 Size: 32 6629 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6661 XbaI T'CTAG_A Cuts at: 3997 3997 Size: 6661 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6661 XmaI C'CCGG_G Cuts at: 1870 3986 1870 Size: 2116 4545 XmnI GAAnn'nnTTC Cuts at: 2712 4790 2712 Size: 2078 4583 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4469 276 Size: 53 83 186 936 2935 2468 Fragments arranged by size: 2935 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI