(Circular) (Six-base) MAPSORT of: pFN21ASDB1489.seq Check: 567 from: 1 to: 9283 pFN21ASDB1489 9283 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2239 2770 3661 3868 3909 2239 Size: 531 891 207 41 7613 Fragments arranged by size: 7613 891 531 207 41 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7093 278 Size: 53 83 186 936 5557 2468 Fragments arranged by size: 5557 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6626 1937 Size: 4689 4594 Acc65I G'GTAC_C Cuts at: 6604 6604 Size: 9283 AclI AA'CG_TT Cuts at: 1563 7410 7783 1563 Size: 5847 373 3063 Fragments arranged by size: 5847 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 6018 7346 8730 8998 1632 Size: 4386 1328 1384 268 1917 Fragments arranged by size: 4386 1917 1384 1328 268 AfeI AGC'GCT Cuts at: 3812 4931 3812 Size: 1119 8164 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9086 AflIII A'CryG_T Cuts at: 1203 3290 5796 8182 9159 1203 Size: 2087 2506 2386 977 1327 Fragments arranged by size: 2506 2386 2087 1327 977 AgeI A'CCGG_T Cuts at: 8114 8114 Size: 9283 AhdI GACnn_n'nnGTC Cuts at: 8012 8012 Size: 9283 AleI CACnn'nnGTG Cuts at: 1166 3785 5420 1166 Size: 2619 1635 5029 Fragments arranged by size: 5029 2619 1635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4666 4676 1184 Size: 3482 10 5791 Fragments arranged by size: 5791 3482 10 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4183 4215 4183 Size: 32 9251 AlwNI CAG_nnn'CTG Cuts at: 2103 2631 3467 3512 4244 4355 4510 4552 5022 Size: 528 836 45 732 111 155 42 470 Cuts at: 5022 5539 5861 7026 8598 2103 Size: 517 322 1165 1572 2788 Fragments arranged by size: 2788 1572 1165 836 732 528 517 470 322 155 111 45 42 ApaI G_GGCC'C Cuts at: 2753 3110 3173 4187 5069 5687 5950 6050 2753 Size: 357 63 1014 882 618 263 100 5986 Fragments arranged by size: 5986 1014 882 618 357 263 100 63 ApaLI G'TGCA_C Cuts at: 2909 3896 4807 5423 6388 7340 8496 2909 Size: 987 911 616 965 952 1156 3696 Fragments arranged by size: 3696 1156 987 965 952 911 616 ApoI r'AATT_y Cuts at: 1493 6592 6818 6970 9079 1493 Size: 5099 226 152 2109 1697 Fragments arranged by size: 5099 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7837 160 Size: 872 6805 1606 Fragments arranged by size: 6805 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9283 AvaI C'yCGr_G Cuts at: 1870 1943 2119 2173 2327 2744 2782 3210 3227 Size: 73 176 54 154 417 38 428 17 Cuts at: 3227 4338 4785 6031 6128 6361 6498 6608 1870 Size: 1111 447 1246 97 233 137 110 4545 Fragments arranged by size: 4545 1246 1111 447 428 417 233 176 154 137 110 97 73 54 38 17 BamHI G'GATC_C Cuts at: 4432 6613 6980 4432 Size: 2181 367 6735 Fragments arranged by size: 6735 2181 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2506 2740 3194 3362 4195 Size: 325 217 627 719 234 454 168 833 Cuts at: 4195 5184 5731 6438 6604 8059 618 Size: 989 547 707 166 1455 1842 Fragments arranged by size: 1842 1455 989 833 719 707 627 547 454 325 234 217 168 166 BanII G_rGCy'C Cuts at: 1391 2484 2753 3110 3173 3965 4011 4187 4389 Size: 1093 269 357 63 792 46 176 202 Cuts at: 4389 4407 4613 5069 5291 5667 5687 5950 6050 Size: 18 206 456 222 376 20 263 100 Cuts at: 6050 6194 6285 6482 6540 6602 1391 Size: 144 91 197 58 62 4072 Fragments arranged by size: 4072 1093 792 456 376 357 269 263 222 206 202 197 176 144 100 91 63 62 58 46 20 18 BbeI G_GCGC'C Cuts at: 2510 6442 2510 Size: 3932 5351 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5107 5380 5505 928 Size: 971 3208 273 125 4706 Fragments arranged by size: 4706 3208 971 273 125 BbvCI CC'TCA_GC Cuts at: 2766 5699 2766 Size: 2933 6350 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3208 3242 4900 4934 7474 7508 1903 Size: 34 1271 34 1658 34 2540 34 3678 Fragments arranged by size: 3678 2540 1658 1271 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4997 7175 8385 4997 Size: 2178 1210 5895 Fragments arranged by size: 5895 2178 1210 BclI T'GATC_A Cuts at: 1548 3990 1548 Size: 2442 6841 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2627 4059 4315 Size: 107 122 71 631 605 955 1432 256 Cuts at: 4315 5339 5682 5691 5945 7894 136 Size: 1024 343 9 254 1949 1525 Fragments arranged by size: 1949 1525 1432 1024 955 631 605 343 256 254 122 107 71 9 BglII A'GATC_T Cuts at: 9278 9278 Size: 9283 BlpI GC'TnA_GC Cuts at: 2478 2643 3873 4118 4383 4793 5215 5246 5251 Size: 165 1230 245 265 410 422 31 5 Cuts at: 5251 6700 2478 Size: 1449 5061 Fragments arranged by size: 5061 1449 1230 422 410 265 245 165 31 5 Bme1580I G_kGCm'C Cuts at: 1790 2745 2753 2913 3000 3110 3173 3199 3236 Size: 955 8 160 87 110 63 26 37 Cuts at: 3236 3341 3365 3494 3541 3792 3900 4187 4811 Size: 105 24 129 47 251 108 287 624 Cuts at: 4811 5069 5189 5427 5630 5687 5736 5950 6050 Size: 258 120 238 203 57 49 214 100 Cuts at: 6050 6129 6171 6392 7344 8500 1790 Size: 79 42 221 952 1156 2573 Fragments arranged by size: 2573 1156 955 952 624 287 258 251 238 221 214 203 160 129 120 110 108 105 100 87 79 63 57 49 47 42 37 26 24 8 BmgBI CAC'GTC Cuts at: 1318 3120 3291 3450 1318 Size: 1802 171 159 7151 Fragments arranged by size: 7151 1802 171 159 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2091 2854 3565 Size: 416 53 83 399 24 674 763 711 Cuts at: 3565 4081 4495 6485 7014 7972 442 Size: 516 414 1990 529 958 1753 Fragments arranged by size: 1990 1753 958 763 711 674 529 516 416 414 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9283 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4030 5875 4030 Size: 1845 7438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2617 2685 2959 4362 4444 4532 4619 Size: 974 404 68 274 1403 82 88 87 Cuts at: 4619 5057 5305 6050 6218 6472 7943 1239 Size: 438 248 745 168 254 1471 2579 Fragments arranged by size: 2579 1471 1403 974 745 438 404 274 254 248 168 88 87 82 68 Bpu10I CC'TnA_GC Cuts at: 2419 2766 3033 4477 5699 2419 Size: 347 267 1444 1222 6003 Fragments arranged by size: 6003 1444 1222 347 267 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3932 7408 8273 8571 3932 Size: 3476 865 298 4644 Fragments arranged by size: 4644 3476 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4508 7946 882 Size: 617 3009 3438 2219 Fragments arranged by size: 3438 3009 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3436 3787 5135 6580 7074 493 Size: 711 2232 351 1348 1445 494 2702 Fragments arranged by size: 2702 2232 1445 1348 711 494 351 BsaBI GATnn'nnATC Cuts at: 5562 9277 5562 Size: 3715 5568 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2507 6439 7090 7472 Size: 53 83 186 936 974 3932 651 382 Cuts at: 7472 8107 275 Size: 635 1451 Fragments arranged by size: 3932 1451 974 936 651 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3365 6550 7716 8114 8388 8535 8946 3365 Size: 3185 1166 398 274 147 411 3702 Fragments arranged by size: 3702 3185 1166 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2237 2267 2683 2713 2918 Size: 30 165 30 834 30 416 30 205 Cuts at: 2918 2948 3360 3390 4183 4213 4360 4390 5654 Size: 30 412 30 793 30 147 30 1264 Cuts at: 5654 5684 6332 6362 1178 Size: 30 648 30 4099 Fragments arranged by size: 4099 1264 834 793 648 416 412 205 165 147 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2345 2390 2393 2618 2843 2939 3386 3521 Size: 1159 45 3 225 225 96 447 135 Cuts at: 3521 4117 4220 4837 5195 5675 1186 Size: 596 103 617 358 480 4794 Fragments arranged by size: 4794 1159 617 596 480 447 358 225 225 135 103 96 45 3 BseYI C'CCAG_C Cuts at: 2029 2752 3003 3172 3566 4234 4371 4449 4575 Size: 723 251 169 394 668 137 78 126 Cuts at: 4575 4857 4947 5234 5755 6008 6043 6110 6401 Size: 282 90 287 521 253 35 67 291 Cuts at: 6401 6419 8486 8982 2029 Size: 18 2067 496 2330 Fragments arranged by size: 2330 2067 723 668 521 496 394 291 287 282 253 251 169 137 126 90 78 67 35 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2423 2431 2480 3178 3238 3442 3827 Size: 573 687 8 49 698 60 204 385 Cuts at: 3827 4662 4814 6500 1163 Size: 835 152 1686 3946 Fragments arranged by size: 3946 1686 835 698 687 573 385 204 152 60 49 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5954 6310 6733 7494 8522 1540 Size: 275 186 3953 356 423 761 1028 2301 Fragments arranged by size: 3953 2301 1028 761 423 356 275 186 BsiHKAI G_wGCw'C Cuts at: 2130 2589 2913 2951 3284 3627 3900 3965 4119 Size: 459 324 38 333 343 273 65 154 Cuts at: 4119 4407 4811 5291 5427 6285 6392 6458 7344 Size: 288 404 480 136 858 107 66 886 Cuts at: 7344 7429 8500 2130 Size: 85 1071 2913 Fragments arranged by size: 2913 1071 886 858 480 459 404 343 333 324 288 273 154 136 107 85 66 65 38 BsmI GAATG_Cn' Cuts at: 3271 3505 3996 6262 6794 6887 3271 Size: 234 491 2266 532 93 5667 Fragments arranged by size: 5667 2266 532 491 234 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4018 4018 Size: 9283 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2484 2589 2745 2753 2913 2951 Size: 399 340 354 105 156 8 160 38 Cuts at: 2951 3000 3110 3173 3199 3236 3284 3341 3365 Size: 49 110 63 26 37 48 57 24 Cuts at: 3365 3494 3541 3627 3792 3900 3965 4011 4119 Size: 129 47 86 165 108 65 46 108 Cuts at: 4119 4187 4389 4407 4613 4811 5069 5189 5291 Size: 68 202 18 206 198 258 120 102 Cuts at: 5291 5427 5630 5667 5687 5736 5950 6050 6129 Size: 136 203 37 20 49 214 100 79 Cuts at: 6129 6171 6194 6285 6392 6458 6482 6540 6602 Size: 42 23 91 107 66 24 58 62 Cuts at: 6602 7344 7429 8500 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 354 340 258 214 206 203 202 198 165 160 156 136 129 120 110 108 108 107 105 102 100 91 86 85 79 68 66 65 63 62 58 57 49 49 48 47 46 42 38 37 37 26 24 24 23 20 18 8 BspEI T'CCGG_A Cuts at: 8946 8946 Size: 9283 BspHI T'CATG_A Cuts at: 7172 8902 7172 Size: 1730 7553 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2235 2239 2318 2770 2999 3661 3735 3868 Size: 1391 4 79 452 229 662 74 133 Cuts at: 3868 3909 6638 844 Size: 41 2729 3489 Fragments arranged by size: 3489 2729 1391 662 452 229 133 79 74 41 4 BsrBI CCG'CTC Cuts at: 1394 1823 5182 5228 6379 7170 1394 Size: 429 3359 46 1151 791 3507 Fragments arranged by size: 3507 3359 1151 791 429 46 BsrDI GCAATG_nn' Cuts at: 65 1248 2800 4438 7778 7952 65 Size: 1183 1552 1638 3340 174 1396 Fragments arranged by size: 3340 1638 1552 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2621 2678 7927 8114 1700 Size: 110 811 57 5249 187 2869 Fragments arranged by size: 5249 2869 811 187 110 57 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9283 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9283 BssSI C'ACGA_G Cuts at: 4772 5512 5678 6278 7343 8355 4772 Size: 740 166 600 1065 1012 5700 Fragments arranged by size: 5700 1065 1012 740 600 166 BstAPI GCAn_nnn'nTGC Cuts at: 794 2264 2631 2908 3021 3646 3676 794 Size: 1470 367 277 113 625 30 6401 Fragments arranged by size: 6401 1470 625 367 277 113 30 BstBI TT'CG_AA Cuts at: 2007 8087 2007 Size: 6080 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9283 BstXI CCAn_nnnn'nTGG Cuts at: 2737 5986 6187 2737 Size: 3249 201 5833 Fragments arranged by size: 5833 3249 201 BstYI r'GATC_y Cuts at: 1972 4432 5535 5563 6059 6613 6980 7365 7382 Size: 2460 1103 28 496 554 367 385 17 Cuts at: 7382 8834 8920 8932 9278 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2460 1977 1452 1103 554 496 385 367 346 86 28 17 12 Bsu36I CC'TnA_GG Cuts at: 3505 5688 3505 Size: 2183 7100 BtgI C'CryG_G Cuts at: 513 1065 3260 3333 4428 5271 5736 6021 6327 Size: 552 2195 73 1095 843 465 285 306 Cuts at: 6327 513 Size: 3469 Fragments arranged by size: 3469 2195 1095 843 552 465 306 285 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2152 3954 487 Size: 683 56 926 1802 5816 Fragments arranged by size: 5816 1802 926 683 56 BtsI GCAGTG_nn' Cuts at: 4650 5415 5425 5711 6799 7593 7613 4650 Size: 765 10 286 1088 794 20 6320 Fragments arranged by size: 6320 1088 794 765 286 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9283 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9248 DraI TTT'AAA Cuts at: 6587 6587 Size: 9283 DraIII CAC_nnn'GTG Cuts at: 2917 3934 2917 Size: 1017 8266 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8290 809 Size: 507 6974 1802 Fragments arranged by size: 6974 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2366 2611 Size: 54 1425 50 275 18 243 293 245 Cuts at: 2611 2864 3237 3630 4326 4971 5259 5951 6330 Size: 253 373 393 696 645 288 692 379 Cuts at: 6330 6730 7619 9248 8 Size: 400 889 1629 43 Fragments arranged by size: 1629 1425 889 696 692 645 400 393 379 373 293 288 275 253 245 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5951 6730 1537 Size: 275 4139 779 4090 Fragments arranged by size: 4139 4090 779 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2367 2832 3389 3737 4103 5372 7213 1353 Size: 1014 465 557 348 366 1269 1841 3423 Fragments arranged by size: 3423 1841 1269 1014 557 465 366 348 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2363 4918 5757 5905 7870 8244 Size: 1064 138 774 2555 839 148 1965 374 Cuts at: 8244 8390 387 Size: 146 1280 Fragments arranged by size: 2555 1965 1280 1064 839 774 374 148 146 138 EcoICRI GAG'CTC Cuts at: 3963 4405 5289 6283 3963 Size: 442 884 994 6963 Fragments arranged by size: 6963 994 884 442 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2617 2685 2959 4362 4444 4532 Size: 393 581 404 68 274 1403 82 88 Cuts at: 4532 4619 5057 5305 6018 6050 6218 6472 7346 Size: 87 438 248 713 32 168 254 874 Cuts at: 7346 7943 8730 8998 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1403 874 787 713 597 581 438 404 393 274 268 254 248 168 88 87 82 68 32 EcoNI CCTnn'n_nnAGG Cuts at: 3632 3772 4106 4206 4693 5260 5369 5651 3632 Size: 140 334 100 487 567 109 282 7264 Fragments arranged by size: 7264 567 487 334 282 140 109 100 EcoO109I rG'GnC_Cy Cuts at: 2136 2152 2423 2491 2628 2705 2749 2934 2970 Size: 16 271 68 137 77 44 185 36 Cuts at: 2970 3029 3098 3106 3135 3724 4183 4317 4588 Size: 59 69 8 29 589 459 134 271 Cuts at: 4588 5066 5374 5684 5946 5961 6047 2136 Size: 478 308 310 262 15 86 5372 Fragments arranged by size: 5372 589 478 459 310 308 271 271 262 185 137 134 86 77 69 68 59 44 36 29 16 15 8 EcoRI G'AATT_C Cuts at: 6592 6970 6592 Size: 378 8905 FspI TGC'GCA Cuts at: 2257 3680 4910 7789 2257 Size: 1423 1230 2879 3751 Fragments arranged by size: 3751 2879 1423 1230 FspAI rTGC'GCAy Cuts at: 2257 4910 2257 Size: 2653 6630 HaeII r_GCGC'y Cuts at: 1678 2510 3313 3814 4228 4661 4933 5318 6117 Size: 832 803 501 414 433 272 385 799 Cuts at: 6117 6418 6442 8430 1678 Size: 301 24 1988 2531 Fragments arranged by size: 2531 1988 832 803 799 501 433 414 385 301 272 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2765 2797 2918 2950 3650 Size: 32 135 32 1193 32 121 32 700 Cuts at: 3650 3682 3996 4028 4238 4270 4417 4449 4460 Size: 32 314 32 210 32 147 32 11 Cuts at: 4460 4492 5129 5161 5654 5686 6142 6174 7924 Size: 32 637 32 493 32 456 32 1750 Cuts at: 7924 7956 7998 8030 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1750 1193 700 637 493 456 314 210 147 135 121 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 2302 5599 6627 6873 669 Size: 988 281 364 3297 1028 246 3079 Fragments arranged by size: 3297 3079 1028 988 364 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9283 HpaI GTT'AAC Cuts at: 6873 6873 Size: 9283 KasI G'GCGC_C Cuts at: 2506 6438 2506 Size: 3932 5351 KpnI G_GTAC'C Cuts at: 6608 6608 Size: 9283 MfeI C'AATT_G Cuts at: 6882 6882 Size: 9283 MluI A'CGCG_T Cuts at: 9159 9159 Size: 9283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2382 4782 5157 5172 6115 8397 8581 1305 Size: 1077 2400 375 15 943 2282 184 2007 Fragments arranged by size: 2400 2282 2007 1077 943 375 184 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 3632 10 Size: 54 1425 343 243 1557 5661 Fragments arranged by size: 5661 1557 1425 343 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 3785 4397 5324 5420 6185 7241 7600 Size: 648 2619 612 927 96 765 1056 359 Cuts at: 7600 7759 518 Size: 159 2042 Fragments arranged by size: 2619 2042 1056 927 765 648 612 359 159 96 MspA1I CmG'CkG Cuts at: 1606 2396 2415 2464 2513 2595 2658 2958 3464 Size: 790 19 49 49 82 63 300 506 Cuts at: 3464 3525 4019 4324 4382 4449 4536 5530 5824 Size: 61 494 305 58 67 87 994 294 Cuts at: 5824 6699 7376 8524 8769 1606 Size: 875 677 1148 245 2120 Fragments arranged by size: 2120 1148 994 875 790 677 506 494 305 300 294 245 87 82 67 63 61 58 49 49 19 NaeI GCC'GGC Cuts at: 2623 2680 2623 Size: 57 9226 NarI GG'CG_CC Cuts at: 2507 6439 2507 Size: 3932 5351 NcoI C'CATG_G Cuts at: 513 1065 3260 5271 5736 513 Size: 552 2195 2011 465 4060 Fragments arranged by size: 4060 2195 2011 552 465 NdeI CA'TA_TG Cuts at: 387 387 Size: 9283 NgoMIV G'CCGG_C Cuts at: 2621 2678 2621 Size: 57 9226 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9283 NotI GC'GGCC_GC Cuts at: 6730 6730 Size: 9283 NspI r_CATG'y Cuts at: 1132 1229 3189 3325 3649 3667 5098 5800 6641 Size: 97 1960 136 324 18 1431 702 841 Cuts at: 6641 8186 1132 Size: 1545 2229 Fragments arranged by size: 2229 1960 1545 1431 841 702 324 136 97 18 PasI CC'CwG_GG Cuts at: 1348 3102 4133 5190 5943 6407 1348 Size: 1754 1031 1057 753 464 4224 Fragments arranged by size: 4224 1754 1057 1031 753 464 PciI A'CATG_T Cuts at: 5796 8182 5796 Size: 2386 6897 PflMI CCAn_nnn'nTGG Cuts at: 1108 2296 3547 4510 5455 5717 6554 1108 Size: 1188 1251 963 945 262 837 3837 Fragments arranged by size: 3837 1251 1188 963 945 837 262 PfoI T'CCnGG_A Cuts at: 2183 2936 2972 5448 2183 Size: 753 36 2476 6018 Fragments arranged by size: 6018 2476 753 36 PmeI GTTT'AAAC Cuts at: 6587 6587 Size: 9283 PmlI CAC'GTG Cuts at: 5135 5135 Size: 9283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4183 4215 7352 7384 8865 8897 4183 Size: 32 3137 32 1481 32 4569 Fragments arranged by size: 4569 3137 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2152 2934 2970 3029 3098 3135 3724 4588 5961 Size: 782 36 59 69 37 589 864 1373 Cuts at: 5961 2152 Size: 5474 Fragments arranged by size: 5474 1373 864 782 589 69 59 37 36 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9283 PsiI TTA'TAA Cuts at: 6853 6853 Size: 9283 PspOMI G'GGCC_C Cuts at: 2749 3106 3169 4183 5065 5683 5946 6046 2749 Size: 357 63 1014 882 618 263 100 5986 Fragments arranged by size: 5986 1014 882 618 357 263 100 63 PspXI vC'TCGA_Gb Cuts at: 3227 3227 Size: 9283 PstI C_TGCA'G Cuts at: 830 2151 2657 2878 3042 3056 3690 5074 5535 Size: 1321 506 221 164 14 634 1384 461 Cuts at: 5535 5907 6635 830 Size: 372 728 3478 Fragments arranged by size: 3478 1384 1321 728 634 506 461 372 221 164 14 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9283 PvuII CAG'CTG Cuts at: 2464 2658 2958 3464 4019 4382 4449 5530 5824 Size: 194 300 506 555 363 67 1081 294 Cuts at: 5824 2464 Size: 5923 Fragments arranged by size: 5923 1081 555 506 363 300 294 194 67 SacI G_AGCT'C Cuts at: 3965 4407 5291 6285 3965 Size: 442 884 994 6963 Fragments arranged by size: 6963 994 884 442 SalI G'TCGA_C Cuts at: 1936 6625 1936 Size: 4689 4594 SanDI GG'GwC_CC Cuts at: 2970 3098 3135 5961 2970 Size: 128 37 2826 6292 Fragments arranged by size: 6292 2826 128 37 SapI GCTCTTCn'nnn_ Cuts at: 3737 3737 Size: 9283 SbfI CC_TGCA'GG Cuts at: 3690 6635 3690 Size: 2945 6338 ScaI AGT'ACT Cuts at: 2216 7531 9243 2216 Size: 5315 1712 2256 Fragments arranged by size: 5315 2256 1712 SexAI A'CCwGG_T Cuts at: 2295 3930 2295 Size: 1635 7648 SfcI C'TryA_G Cuts at: 826 1045 2147 2653 2874 3038 3052 3686 3715 Size: 219 1102 506 221 164 14 634 29 Cuts at: 3715 5070 5531 5903 6631 7766 8447 8638 826 Size: 1355 461 372 728 1135 681 191 1471 Fragments arranged by size: 1471 1355 1135 1102 728 681 634 506 461 372 221 219 191 164 29 14 SfoI GGC'GCC Cuts at: 2508 6440 2508 Size: 3932 5351 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9283 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9283 SmaI CCC'GGG Cuts at: 1872 2746 3212 4340 4787 6610 1872 Size: 874 466 1128 447 1823 4545 Fragments arranged by size: 4545 1823 1128 874 466 447 SmlI C'TyrA_G Cuts at: 820 1017 1943 3227 3947 7387 8288 8550 820 Size: 197 926 1284 720 3440 901 262 1553 Fragments arranged by size: 3440 1553 1284 926 901 720 262 197 SnaBI TAC'GTA Cuts at: 493 6580 7074 493 Size: 6087 494 2702 Fragments arranged by size: 6087 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9283 SphI G_CATG'C Cuts at: 1132 3649 6641 1132 Size: 2517 2992 3774 Fragments arranged by size: 3774 2992 2517 SspI AAT'ATT Cuts at: 5 52 7207 5 Size: 47 7155 2081 Fragments arranged by size: 7155 2081 47 StuI AGG'CCT Cuts at: 1517 3020 3953 4874 1517 Size: 1503 933 921 5926 Fragments arranged by size: 5926 1503 933 921 StyI C'CwwG_G Cuts at: 513 1065 3260 3541 3633 4107 4291 4584 5271 Size: 552 2195 281 92 474 184 293 687 Cuts at: 5271 5736 6722 513 Size: 465 986 3074 Fragments arranged by size: 3074 2195 986 687 552 474 465 293 281 184 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4063 4609 4849 6124 7310 7327 7480 Size: 1255 2483 546 240 1275 1186 17 153 Cuts at: 7480 7665 8110 325 Size: 185 445 1498 Fragments arranged by size: 2483 1498 1275 1255 1186 546 445 240 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 4276 4567 Size: 274 80 33 51 1443 237 2062 291 Cuts at: 4567 7529 9241 96 Size: 2962 1712 138 Fragments arranged by size: 2962 2062 1712 1443 291 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2237 2269 2683 2711 2715 2743 3068 3100 3360 Size: 32 414 28 4 28 325 32 260 Cuts at: 3360 3392 6332 6364 9243 9275 2237 Size: 32 2940 32 2879 32 2245 Fragments arranged by size: 2940 2879 2245 414 325 260 32 32 32 32 32 28 28 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9283 XbaI T'CTAG_A Cuts at: 6619 6619 Size: 9283 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2576 3267 4321 5269 6074 2576 Size: 691 1054 948 805 5785 Fragments arranged by size: 5785 1054 948 805 691 XhoI C'TCGA_G Cuts at: 1943 3227 1943 Size: 1284 7999 XmaI C'CCGG_G Cuts at: 1870 2744 3210 4338 4785 6608 1870 Size: 874 466 1128 447 1823 4545 Fragments arranged by size: 4545 1823 1128 874 466 447 XmnI GAAnn'nnTTC Cuts at: 7412 7412 Size: 9283 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7091 276 Size: 53 83 186 936 5557 2468 Fragments arranged by size: 5557 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI FseI NruI NsiI PacI PsrI RsrII SacII SfiI SrfI SwaI