(Circular) (Six-base) MAPSORT of: pFN21AB9574.seq Check: 8646 from: 1 to: 5845 pFN21AB9574 5845 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3188 1937 Size: 1251 4594 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 2816 3908 5292 5560 1632 Size: 953 231 1092 1384 268 1917 Fragments arranged by size: 1917 1384 1092 953 268 231 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 3015 4744 5721 1203 Size: 1812 1729 977 1327 Fragments arranged by size: 1812 1729 1327 977 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 2981 1166 Size: 1815 4030 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2479 1184 Size: 1295 4550 AlwNI CAG_nnn'CTG Cuts at: 2230 3588 5160 2230 Size: 1358 1572 2915 Fragments arranged by size: 2915 1572 1358 ApaI G_GGCC'C Cuts at: 2949 2949 Size: 5845 ApaLI G'TGCA_C Cuts at: 2974 3902 5058 2974 Size: 928 1156 3761 Fragments arranged by size: 3761 1156 928 ApoI r'AATT_y Cuts at: 1493 3154 3380 3532 5641 1493 Size: 1661 226 152 2109 1697 Fragments arranged by size: 2109 1697 1661 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 2014 3041 3170 1870 Size: 73 71 1027 129 4545 Fragments arranged by size: 4545 1027 129 73 71 BamHI G'GATC_C Cuts at: 3175 3542 3175 Size: 367 5478 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3166 4621 618 Size: 325 217 627 1379 1455 1842 Fragments arranged by size: 1842 1455 1379 627 325 217 BanII G_rGCy'C Cuts at: 1391 2129 2281 2751 2808 2949 2970 3164 1391 Size: 738 152 470 57 141 21 194 4072 Fragments arranged by size: 4072 738 470 194 152 141 57 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2348 2804 3012 3022 928 Size: 971 449 456 208 10 3751 Fragments arranged by size: 3751 971 456 449 208 10 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2595 2629 4036 4070 1903 Size: 34 658 34 1407 34 3678 Fragments arranged by size: 3678 1407 658 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4456 136 Size: 107 122 71 631 605 2784 1525 Fragments arranged by size: 2784 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5840 5840 Size: 5845 BlpI GC'TnA_GC Cuts at: 3119 3262 3119 Size: 143 5702 Bme1580I G_kGCm'C Cuts at: 1790 2949 2978 3906 5062 1790 Size: 1159 29 928 1156 2573 Fragments arranged by size: 2573 1159 1156 928 29 BmgBI CAC'GTC Cuts at: 1318 3018 1318 Size: 1700 4145 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2685 2838 2920 Size: 416 53 83 399 24 1268 153 82 Cuts at: 2920 3576 4534 442 Size: 656 958 1753 Fragments arranged by size: 1753 1268 958 656 416 399 153 83 82 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3067 3124 3067 Size: 57 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 2155 2308 2388 2984 4505 1239 Size: 862 54 153 80 596 1521 2579 Fragments arranged by size: 2579 1521 862 596 153 80 54 Bpu10I CC'TnA_GC Cuts at: 2745 2745 Size: 5845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3970 4835 5133 3970 Size: 865 298 4682 Fragments arranged by size: 4682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4508 882 Size: 617 3009 2219 Fragments arranged by size: 3009 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3636 493 Size: 711 2432 2702 Fragments arranged by size: 2702 2432 711 BsaBI GATnn'nnATC Cuts at: 5839 5839 Size: 5845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2039 3652 4034 4669 Size: 53 83 186 936 506 1613 382 635 Cuts at: 4669 275 Size: 1451 Fragments arranged by size: 1613 1451 936 635 506 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2215 2472 2980 4278 4676 4950 5097 5508 2215 Size: 257 508 1298 398 274 147 411 2552 Fragments arranged by size: 2552 1298 508 411 398 274 257 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2099 2129 2915 2945 2957 Size: 30 165 30 696 30 786 30 12 Cuts at: 2957 2980 2987 3010 1178 Size: 23 7 23 4013 Fragments arranged by size: 4013 786 696 165 30 30 30 30 23 23 12 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2242 2491 2723 2726 1186 Size: 1056 249 232 3 4305 Fragments arranged by size: 4305 1056 249 232 3 BseYI C'CCAG_C Cuts at: 2312 2827 2999 3059 5048 5544 2312 Size: 515 172 60 1989 496 2613 Fragments arranged by size: 2613 1989 515 496 172 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2137 2255 2906 2989 3087 1163 Size: 573 401 118 651 83 98 3921 Fragments arranged by size: 3921 651 573 401 118 98 83 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2073 3295 4056 5084 1540 Size: 275 186 72 1222 761 1028 2301 Fragments arranged by size: 2301 1222 1028 761 275 186 72 BsiHKAI G_wGCw'C Cuts at: 2978 3906 3991 5062 2978 Size: 928 85 1071 3761 Fragments arranged by size: 3761 1071 928 85 BsmI GAATG_Cn' Cuts at: 3356 3449 3356 Size: 93 5752 BsmBI CGTCTCn'nnnn_ Cuts at: 2173 2254 2173 Size: 81 5764 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2281 2751 2808 2949 2970 2978 Size: 399 339 152 470 57 141 21 8 Cuts at: 2978 3164 3906 3991 5062 1391 Size: 186 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 470 399 339 186 152 141 85 57 21 8 BspEI T'CCGG_A Cuts at: 2215 5508 2215 Size: 3293 2552 BspHI T'CATG_A Cuts at: 3734 5464 3734 Size: 1730 4115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2229 2568 2617 3200 844 Size: 1385 339 49 583 3489 Fragments arranged by size: 3489 1385 583 339 49 BsrBI CCG'CTC Cuts at: 1394 1823 2865 3732 1394 Size: 429 1042 867 3507 Fragments arranged by size: 3507 1042 867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3103 4340 4514 65 Size: 1183 1855 1237 174 1396 Fragments arranged by size: 1855 1396 1237 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4489 4676 1700 Size: 110 2679 187 2869 Fragments arranged by size: 2869 2679 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5845 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5845 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 2986 1184 Size: 1802 4043 BstXI CCAn_nnnn'nTGG Cuts at: 2962 3136 2962 Size: 174 5671 BstYI r'GATC_y Cuts at: 1972 2619 3175 3542 3927 3944 5396 5482 5494 Size: 647 556 367 385 17 1452 86 12 Cuts at: 5494 5840 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 647 556 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2190 2256 2949 513 Size: 552 1125 66 693 3409 Fragments arranged by size: 3409 1125 693 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2250 487 Size: 683 56 1024 4082 Fragments arranged by size: 4082 1024 683 56 BtsI GCAGTG_nn' Cuts at: 2196 2231 3006 3361 4155 4175 2196 Size: 35 775 355 794 20 3866 Fragments arranged by size: 3866 794 775 355 35 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2165 4852 809 Size: 507 849 2687 1802 Fragments arranged by size: 2687 1802 849 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2163 2952 Size: 54 1425 50 275 18 240 93 789 Cuts at: 2952 3292 4181 5810 8 Size: 340 889 1629 43 Fragments arranged by size: 1629 1425 889 789 340 275 240 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2070 3292 1537 Size: 275 258 1222 4090 Fragments arranged by size: 4090 1222 275 258 EarI CTCTTCn'nnn_ Cuts at: 1353 2409 2640 3775 1353 Size: 1056 231 1135 3423 Fragments arranged by size: 3423 1135 1056 231 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2155 2308 2388 2585 2816 2984 Size: 393 469 54 153 80 197 231 168 Cuts at: 2984 3908 4505 5292 5560 1239 Size: 924 597 787 268 1524 Fragments arranged by size: 1524 924 787 597 469 393 268 231 197 168 153 80 54 EcoNI CCTnn'n_nnAGG Cuts at: 2564 2687 2564 Size: 123 5722 EcoO109I rG'GnC_Cy Cuts at: 2680 2892 2945 2680 Size: 212 53 5580 Fragments arranged by size: 5580 212 53 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 FspI TGC'GCA Cuts at: 4351 4351 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 4992 1678 Size: 3314 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2069 2099 2101 Size: 32 135 32 455 32 10 30 2 Cuts at: 2101 2131 2516 2548 4486 4518 4560 4592 1373 Size: 30 385 32 1938 32 42 32 2626 Fragments arranged by size: 2626 1938 455 385 135 42 32 32 32 32 32 32 30 30 10 2 HincII GTy'rAC Cuts at: 669 1657 1938 2137 3189 3435 669 Size: 988 281 199 1052 246 3079 Fragments arranged by size: 3079 1052 988 281 246 199 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4959 5143 1305 Size: 3654 184 2007 Fragments arranged by size: 3654 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2954 10 Size: 54 1425 343 1122 2901 Fragments arranged by size: 2901 1425 1122 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2981 3803 4162 4321 518 Size: 648 1815 822 359 159 2042 Fragments arranged by size: 2042 1815 822 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2052 2312 3003 3063 3071 3261 3938 5086 Size: 446 260 691 60 8 190 677 1148 Cuts at: 5086 5331 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 691 677 446 260 245 190 60 8 NcoI C'CATG_G Cuts at: 513 1065 2949 513 Size: 552 1884 3409 Fragments arranged by size: 3409 1884 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 3203 4748 1132 Size: 97 1974 1545 2229 Fragments arranged by size: 2229 1974 1545 97 PasI CC'CwG_GG Cuts at: 1348 2935 1348 Size: 1587 4258 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5845 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2892 2892 Size: 5845 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PspOMI G'GGCC_C Cuts at: 2945 2945 Size: 5845 PstI C_TGCA'G Cuts at: 830 2057 2226 2458 3197 830 Size: 1227 169 232 739 3478 Fragments arranged by size: 3478 1227 739 232 169 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 3003 3063 3071 3003 Size: 60 8 5777 Fragments arranged by size: 5777 60 8 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SfcI C'TryA_G Cuts at: 826 1045 2053 2222 2454 3193 4328 5009 5200 Size: 219 1008 169 232 739 1135 681 191 Cuts at: 5200 826 Size: 1471 Fragments arranged by size: 1471 1135 1008 739 681 232 219 191 169 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3949 4850 5112 820 Size: 197 926 2006 901 262 1553 Fragments arranged by size: 2006 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 2717 1517 Size: 1200 4645 StyI C'CwwG_G Cuts at: 513 1065 2949 3284 513 Size: 552 1884 335 3074 Fragments arranged by size: 3074 1884 552 335 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2561 2816 3142 3872 3889 4042 4227 Size: 1255 981 255 326 730 17 153 185 Cuts at: 4227 4672 325 Size: 445 1498 Fragments arranged by size: 1498 1255 981 730 445 326 255 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2993 4091 5803 Size: 274 80 33 51 1443 1016 1098 1712 Cuts at: 5803 96 Size: 138 Fragments arranged by size: 1712 1443 1098 1016 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2210 2242 2915 2947 2957 2980 2989 3012 5805 Size: 32 673 32 10 23 9 23 2793 Cuts at: 5805 5837 2210 Size: 32 2218 Fragments arranged by size: 2793 2218 673 32 32 32 23 23 10 9 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2758 2758 Size: 5845 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI