Restriction map for
pFN21AB9538
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaJI BsiEI BsiWI BsmBI BsoBI
BspDI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MflI MluI MluNI
MscI MspA1I MvaI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
ScrFI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 561 TCCGGA T^CCGGA
AciI 905 GCGG CCGC(-3/-1)
AflII 52 CTTAAG C^TTAAG
AhdI 1016 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1016 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 614 GAGCAC G[AT]GC[AT]^C
BbvCI 327 CCTCAGC CCTCAGC(-5/-2)
BbvI 340 GCAGC GCAGC(8/12)
BfrI 52 CTTAAG C^TTAAG
BmyI 614 GAGCAC G[AGT]GC[ACT]^C
BpmI 455 CTGGAG CTGGAG(16/14)
BsaI 8 GAGACC GGTCTC(1/5)
BsaWI 561 TCCGGA [AT]^CCGG[AT]
BseAI 561 TCCGGA T^CCGGA
BseRI 427 CTCCTC GAGGAG(10/8)
BsiHKAI 614 GAGCAC G[AT]GC[AT]^C
BsmAI 8 GAGAC GTCTC(1/5)
BsmFI 1011 GTCCC GGGAC(10/14)
BsmI 472 GAATGC GAATGC(1/-1)
Bsp1286I 614 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 603 TGTACA T^GTACA
BspEI 561 TCCGGA T^CCGGA
BsrDI 476 GCAATG GCAATG(2/0)
BsrGI 603 TGTACA T^GTACA
Cac8I 30 GCAGGC GC[ACGT]^[ACGT]GC
DpnII 85 GATC ^GATC
DraI 349 TTTAAA TTT^AAA
Eam1105I 1016 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 998 CCTTTAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 562 CCGG C^CGG
HincII 927 GTCAAC GT[CT]^[GA]AC
HindII 927 GTCAAC GT[CT]^[GA]AC
HindIII 575 AAGCTT A^AGCTT
HpaII 562 CCGG C^CGG
MboI 85 GATC ^GATC
MfeI 68 CAATTG C^AATTG
MroI 561 TCCGGA T^CCGGA
MspI 562 CCGG C^CGG
MunI 68 CAATTG C^AATTG
NdeII 85 GATC ^GATC
NlaIII 861 CATG CATG^
PleI 1016 GACTC GAGTC(4/5)
Sau3AI 85 GATC ^GATC
SmlI 52 CTTAAG C^T[CT][GA]AG
SspBI 603 TGTACA T^GTACA
SspI 296 AATATT AAT^ATT
StuI 995 AGGCCT AGG^CCT
TaqI 554 TCGA T^CGA
TseI 340 GCAGC G^C[AT]GC
TthHB8I 554 TCGA T^CGA
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# TWO CUT ENZYMES ( 12 enzymes) #
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Asp700I 489 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
546 GAAACCTTTC
BsgI 642 GTGCAG GTGCAG(16/14)
810 GTGCAG
EcoRI 530 GAATTC G^AATTC
698 GAATTC
Fnu4HI 340 GCAGC GC^[ACGT]GC
905 GCGGC
HaeIII 507 GGCC GG^CC
996 GGCC
HphI 581 TCACC GGTGA(8/7)
683 TCACC
ItaI 340 GCAGC GC^[ACGT]GC
905 GCGGC
MboII 627 GAAGA GAAGA(8/7)
780 GAAGA
MwoI 30 GCAGGCGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
803 GCTATGAGTGC
TfiI 783 GATTC G^A[AT]TC
950 GAATC
Tsp45I 401 GTGAC ^GT[GC]AC
423 GTCAC
XmnI 489 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
546 GAAACCTTTC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
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AcsI AfaI AluI ApoI BsiYI
BslI BsrI DdeI HinfI MaeIII
MnlI MseI MslI NdeI RsaI
Tru9I Tsp509I TspRI