Restriction map for
pFN21AB9522
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AocI Aor51HI
AscI AseI AsnI Asp718I AspI
AvrII BbeI BbrPI BciVI BclI
BfrI BglI BglII BlnI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraI Eco47III
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MunI MvnI
NarI NdeI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 906 GAATTC [GA]^AATT[CT]
AhdI 670 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 393 GGGCCC GGGCC^C
ApoI 906 GAATTC [GA]^AATT[CT]
Asp700I 88 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 670 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 161 TGCGCA TGC^GCA
BalI 1739 TGGCCA TGG^CCA
BamHI 1670 GGATCC G^GATCC
BbvCI 1824 CCTCAGC CCTCAGC(-5/-2)
BsaAI 1222 TACGTG [CT]AC^GT[GA]
BsaWI 692 ACCGGA [AT]^CCGG[AT]
BsiEI 500 CGGCCG CG[GA][CT]^CG
BsmBI 1236 CGTCTC CGTCTC(1/5)
BsrBI 503 CCGCTC CCGCTC(-3/-3)
BstAPI 1764 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 705 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 500 CGGCCG C^GGCCG
Eam1105I 670 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 500 CGGCCG C^GGCCG
Eco52I 500 CGGCCG C^GGCCG
EcoRI 906 GAATTC G^AATTC
FspI 161 TGCGCA TGC^GCA
MaeII 1223 ACGT A^CGT
MluNI 1739 TGGCCA TGG^CCA
MscI 1739 TGGCCA TGG^CCA
NaeI 730 GCCGGC GCC^GGC
NgoMIV 730 GCCGGC G^CCGGC
NheI 1746 GCTAGC G^CTAGC
PaeR7I 1465 CTCGAG C^TCGAG
PvuII 550 CAGCTG CAG^CTG
SacI 532 GAGCTC GAGCT^C
ScaI 791 AGTACT AGT^ACT
StuI 584 AGGCCT AGG^CCT
TfiI 659 GATTC G^A[AT]TC
Tsp509I 907 AATT ^AATT
XcmI 911 CCAGCCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1465 CTCGAG C^TCGAG
XmnI 88 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1186 GACGCC G[GA]^CG[CT]C
1645 GGCGTC
AflIII 524 ACATGT A^C[GA][CT]GT
1803 ACATGT
Alw44I 1192 GTGCAC G^TGCAC
1315 GTGCAC
ApaLI 1192 GTGCAC G^TGCAC
1315 GTGCAC
BcgI 1355 CGAGCTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
164 GCATCTCCATCG
BfaI 350 CTAG C^TAG
1747 CTAG
BsaHI 1186 GACGCC G[GA]^CG[CT]C
1645 GGCGTC
BsaI 1099 GAGACC GGTCTC(1/5)
1167 GGTCTC
BspLU11I 524 ACATGT A^CATGT
1803 ACATGT
BsrDI 1263 CATTGC GCAATG(2/0)
1032 GCAATG
BsrFI 730 GCCGGC [GA]^CCGG[CT]
1374 GCCGGT
BssSI 1353 CACGAG CACGAG(-5/-1)
1489 CTCGTG
BstYI 1670 GGATCC [GA]^GATC[CT]
1766 AGATCC
Cfr10I 730 GCCGGC [GA]^CCGG[CT]
1374 GCCGGT
DrdI 3 GACTCCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1678 GACTCCCTGGTC
FauI 399 CCCGC CCCGC(4/6)
610 CCCGC
HaeII 1540 AGCGCC [GA]GCGC^[CT]
2014 AGCGCC
Hin1I 1186 GACGCC G[GA]^CG[CT]C
1645 GGCGTC
MaeI 350 CTAG C^TAG
1747 CTAG
MflI 1670 GGATCC [GA]^GATC[CT]
1766 AGATCC
NcoI 1544 CCATGG C^CATGG
1754 CCATGG
PpuMI 542 GGGACCT [GA]G^G[AT]CC[CT]
740 AGGACCT
SapI 1998 GAAGAGC GCTCTTC(1/4)
48 GCTCTTC
SmaI 1071 CCCGGG CCC^GGG
1976 CCCGGG
XhoII 1670 GGATCC [GA]^GATC[CT]
1766 AGATCC
XmaCI 1071 CCCGGG C^CCGGG
1976 CCCGGG
XmaI 1071 CCCGGG C^CCGGG
1976 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I