(Circular) (Six-base) MAPSORT of: pFN21AB9522.seq Check: 8962 from: 1 to: 6766 pFN21AB9522 6766 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3458 3458 Size: 6766 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4576 278 Size: 53 83 186 936 3040 2468 Fragments arranged by size: 3040 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4109 1937 Size: 2172 4594 Acc65I G'GTAC_C Cuts at: 4087 4087 Size: 6766 AclI AA'CG_TT Cuts at: 1563 4893 5266 1563 Size: 3330 373 3063 Fragments arranged by size: 3330 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2594 2866 3703 4022 4829 6213 6481 1632 Size: 962 272 837 319 807 1384 268 1917 Fragments arranged by size: 1917 1384 962 837 807 319 272 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6569 AflIII A'CryG_T Cuts at: 1203 2529 3808 5665 6642 1203 Size: 1326 1279 1857 977 1327 Fragments arranged by size: 1857 1327 1326 1279 977 AgeI A'CCGG_T Cuts at: 5597 5597 Size: 6766 AhdI GACnn_n'nnGTC Cuts at: 2680 5495 2680 Size: 2815 3951 AleI CACnn'nnGTG Cuts at: 1166 3761 1166 Size: 2595 4171 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6766 AlwNI CAG_nnn'CTG Cuts at: 2020 2572 2638 2749 3398 3775 4509 6081 2020 Size: 552 66 111 649 377 734 1572 2705 Fragments arranged by size: 2705 1572 734 649 552 377 111 66 ApaI G_GGCC'C Cuts at: 2402 2402 Size: 6766 ApaLI G'TGCA_C Cuts at: 3197 3320 4823 5979 3197 Size: 123 1503 1156 3984 Fragments arranged by size: 3984 1503 1156 123 ApoI r'AATT_y Cuts at: 1493 2911 4075 4301 4453 6562 1493 Size: 1418 1164 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 1164 226 152 AseI AT'TA_AT Cuts at: 160 1032 5320 160 Size: 872 4288 1606 Fragments arranged by size: 4288 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6766 AvaI C'yCGr_G Cuts at: 1870 1943 3076 3300 3470 3981 4039 4091 1870 Size: 73 1133 224 170 511 58 52 4545 Fragments arranged by size: 4545 1133 511 224 170 73 58 52 BamHI G'GATC_C Cuts at: 3675 4096 4463 3675 Size: 421 367 5978 Fragments arranged by size: 5978 421 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 3512 3884 4087 5542 Size: 325 217 627 282 1443 372 203 1455 Cuts at: 5542 618 Size: 1842 Fragments arranged by size: 1842 1455 1443 627 372 325 282 217 203 BanII G_rGCy'C Cuts at: 1391 2402 2441 2541 2988 4085 1391 Size: 1011 39 100 447 1097 4072 Fragments arranged by size: 4072 1097 1011 447 100 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2100 3063 3337 928 Size: 971 201 963 274 4357 Fragments arranged by size: 4357 971 963 274 201 BbvCI CC'TCA_GC Cuts at: 3830 3830 Size: 6766 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2158 2192 3349 3383 4957 4991 1903 Size: 34 221 34 1157 34 1574 34 3678 Fragments arranged by size: 3678 1574 1157 221 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4658 5868 4658 Size: 1210 5556 BclI T'GATC_A Cuts at: 1548 1548 Size: 6766 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5377 136 Size: 107 122 71 631 605 3705 1525 Fragments arranged by size: 3705 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6761 6761 Size: 6766 BlpI GC'TnA_GC Cuts at: 2561 3371 3542 4183 2561 Size: 810 171 641 5144 Fragments arranged by size: 5144 810 641 171 Bme1580I G_kGCm'C Cuts at: 1790 2074 2402 3201 3324 4827 5983 1790 Size: 284 328 799 123 1503 1156 2573 Fragments arranged by size: 2573 1503 1156 799 328 284 123 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6766 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2008 2454 4497 Size: 416 53 83 399 24 591 446 2043 Cuts at: 4497 5455 442 Size: 958 1753 Fragments arranged by size: 2043 1753 958 591 446 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3755 1056 Size: 2699 4067 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2409 3315 2409 Size: 906 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2537 2660 2708 2726 3271 3627 5426 1239 Size: 1298 123 48 18 545 356 1799 2579 Fragments arranged by size: 2579 1799 1298 545 356 123 48 18 Bpu10I CC'TnA_GC Cuts at: 2394 3212 3830 2394 Size: 818 618 5330 Fragments arranged by size: 5330 818 618 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2578 3521 4891 5756 6054 2578 Size: 943 1370 865 298 3290 Fragments arranged by size: 3290 1370 943 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3098 3178 5429 882 Size: 617 1599 80 2251 2219 Fragments arranged by size: 2251 2219 1599 617 80 BsaAI yAC'GTr Cuts at: 493 1204 3229 4557 493 Size: 711 2025 1328 2702 Fragments arranged by size: 2702 2025 1328 711 BsaBI GATnn'nnATC Cuts at: 6760 6760 Size: 6766 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3192 3651 4573 4955 Size: 53 83 186 936 1659 459 922 382 Cuts at: 4955 5590 275 Size: 635 1451 Fragments arranged by size: 1659 1451 936 922 635 459 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2697 5199 5597 5871 6018 6429 2697 Size: 2502 398 274 147 411 3034 Fragments arranged by size: 3034 2502 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2744 2774 3269 3299 1178 Size: 30 165 30 1341 30 495 30 4645 Fragments arranged by size: 4645 1341 495 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 2396 2705 2786 2789 2858 3166 3209 Size: 1165 45 309 81 3 69 308 43 Cuts at: 3209 1186 Size: 4743 Fragments arranged by size: 4743 1165 309 308 81 69 45 43 3 BseYI C'CCAG_C Cuts at: 2297 3095 3555 3823 5969 6465 2297 Size: 798 460 268 2146 496 2598 Fragments arranged by size: 2598 2146 798 496 460 268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2735 3416 3788 1163 Size: 573 999 681 372 4141 Fragments arranged by size: 4141 999 681 573 372 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2508 4216 4977 6005 1540 Size: 275 186 507 1708 761 1028 2301 Fragments arranged by size: 2301 1708 1028 761 507 275 186 BsiHKAI G_wGCw'C Cuts at: 2541 2997 3201 3324 4827 4912 5983 2541 Size: 456 204 123 1503 85 1071 3324 Fragments arranged by size: 3324 1503 1071 456 204 123 85 BsmI GAATG_Cn' Cuts at: 4277 4370 4277 Size: 93 6673 BsmBI CGTCTCn'nnnn_ Cuts at: 3247 3247 Size: 6766 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2402 2441 2541 2988 2997 3201 Size: 399 284 328 39 100 447 9 204 Cuts at: 3201 3324 4085 4827 4912 5983 1391 Size: 123 761 742 85 1071 2174 Fragments arranged by size: 2174 1071 761 742 447 399 328 284 204 123 100 85 39 9 BspEI T'CCGG_A Cuts at: 6429 6429 Size: 6766 BspHI T'CATG_A Cuts at: 4655 6385 4655 Size: 1730 5036 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2172 2757 3339 3458 4121 844 Size: 1328 585 582 119 663 3489 Fragments arranged by size: 3489 1328 663 585 582 119 BsrBI CCG'CTC Cuts at: 1394 1823 2510 4653 1394 Size: 429 687 2143 3507 Fragments arranged by size: 3507 2143 687 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3044 3267 5261 5435 65 Size: 1183 1796 223 1994 174 1396 Fragments arranged by size: 1994 1796 1396 1183 223 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2735 3379 5410 5597 1700 Size: 110 925 644 2031 187 2869 Fragments arranged by size: 2869 2031 925 644 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6766 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6766 BssSI C'ACGA_G Cuts at: 3358 3494 4826 5838 3358 Size: 136 1332 1012 4286 Fragments arranged by size: 4286 1332 1012 136 BstAPI GCAn_nnn'nTGC Cuts at: 794 3775 794 Size: 2981 3785 BstBI TT'CG_AA Cuts at: 5570 5570 Size: 6766 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6766 BstXI CCAn_nnnn'nTGG Cuts at: 3574 3625 3763 3574 Size: 51 138 6577 Fragments arranged by size: 6577 138 51 BstYI r'GATC_y Cuts at: 1972 3675 3771 4096 4463 4848 4865 6317 6403 Size: 1703 96 325 367 385 17 1452 86 Cuts at: 6403 6415 6761 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1703 1452 385 367 346 325 96 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2074 2709 2988 3549 3759 513 Size: 552 1009 635 279 561 210 3520 Fragments arranged by size: 3520 1009 635 561 552 279 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6027 Fragments arranged by size: 6027 683 56 BtsI GCAGTG_nn' Cuts at: 3097 3168 3818 4282 5076 5096 3097 Size: 71 650 464 794 20 4767 Fragments arranged by size: 4767 794 650 464 71 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6766 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6731 DraI TTT'AAA Cuts at: 4070 4070 Size: 6766 DraIII CAC_nnn'GTG Cuts at: 2715 2715 Size: 6766 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2014 3689 5773 809 Size: 507 698 1675 2084 1802 Fragments arranged by size: 2084 1802 1675 698 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2505 3339 3744 Size: 54 1425 50 275 18 675 834 405 Cuts at: 3744 4213 5102 6731 8 Size: 469 889 1629 43 Fragments arranged by size: 1629 1425 889 834 675 469 405 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2505 4213 1537 Size: 275 693 1708 4090 Fragments arranged by size: 4090 1708 693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2184 2828 2855 3722 3998 4696 1353 Size: 707 124 644 27 867 276 698 3423 Fragments arranged by size: 3423 867 707 698 644 276 124 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5353 5727 5873 387 Size: 1064 138 3764 374 146 1280 Fragments arranged by size: 3764 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2539 2539 Size: 6766 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2537 2594 2660 2708 2726 2866 3271 Size: 393 905 57 66 48 18 140 405 Cuts at: 3271 3627 3703 4022 4829 5426 6213 6481 1239 Size: 356 76 319 807 597 787 268 1524 Fragments arranged by size: 1524 905 807 787 597 405 393 356 319 268 140 76 66 57 48 18 EcoNI CCTnn'n_nnAGG Cuts at: 2393 2596 2900 2393 Size: 203 304 6259 Fragments arranged by size: 6259 304 203 EcoO109I rG'GnC_Cy Cuts at: 2194 2260 2398 2399 2548 2601 2746 2194 Size: 66 138 1 149 53 145 6214 Fragments arranged by size: 6214 149 145 138 66 53 1 EcoRI G'AATT_C Cuts at: 2911 4075 4453 2911 Size: 1164 378 5224 Fragments arranged by size: 5224 1164 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2347 2347 Size: 6766 FspI TGC'GCA Cuts at: 2168 5272 2168 Size: 3104 3662 HaeII r_GCGC'y Cuts at: 1678 3549 4023 5913 1678 Size: 1871 474 1890 2531 Fragments arranged by size: 2531 1890 1871 474 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2293 2325 2336 2368 2666 Size: 32 135 32 721 32 11 32 298 Cuts at: 2666 2698 5407 5439 5481 5513 1373 Size: 32 2709 32 42 32 2626 Fragments arranged by size: 2709 2626 721 298 135 42 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 4110 4356 669 Size: 988 281 2172 246 3079 Fragments arranged by size: 3079 2172 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6766 HpaI GTT'AAC Cuts at: 4356 4356 Size: 6766 KpnI G_GTAC'C Cuts at: 4091 4091 Size: 6766 MfeI C'AATT_G Cuts at: 4365 4365 Size: 6766 MluI A'CGCG_T Cuts at: 6642 6642 Size: 6766 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5880 6064 1305 Size: 4575 184 2007 Fragments arranged by size: 4575 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3746 10 Size: 54 1425 343 1914 3030 Fragments arranged by size: 3030 1914 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3317 3746 3761 3764 4724 5083 5242 Size: 648 2151 429 15 3 960 359 159 Cuts at: 5242 518 Size: 2042 Fragments arranged by size: 2151 2042 960 648 429 359 159 15 3 MspA1I CmG'CkG Cuts at: 1606 2116 2407 2557 4182 4859 6007 6252 1606 Size: 510 291 150 1625 677 1148 245 2120 Fragments arranged by size: 2120 1625 1148 677 510 291 245 150 NaeI GCC'GGC Cuts at: 2737 2737 Size: 6766 NcoI C'CATG_G Cuts at: 513 1065 3549 3759 513 Size: 552 2484 210 3520 Fragments arranged by size: 3520 2484 552 210 NdeI CA'TA_TG Cuts at: 387 387 Size: 6766 NgoMIV G'CCGG_C Cuts at: 2735 2735 Size: 6766 NheI G'CTAG_C Cuts at: 1052 3751 1052 Size: 2699 4067 NotI GC'GGCC_GC Cuts at: 4213 4213 Size: 6766 NspI r_CATG'y Cuts at: 1132 1229 2297 2533 3812 4124 5669 1132 Size: 97 1068 236 1279 312 1545 2229 Fragments arranged by size: 2229 1545 1279 1068 312 236 97 PasI CC'CwG_GG Cuts at: 1348 2256 3852 1348 Size: 908 1596 4262 Fragments arranged by size: 4262 1596 908 PciI A'CATG_T Cuts at: 2529 3808 5665 2529 Size: 1279 1857 3630 Fragments arranged by size: 3630 1857 1279 PflMI CCAn_nnn'nTGG Cuts at: 1108 2715 2926 3555 3672 1108 Size: 1607 211 629 117 4202 Fragments arranged by size: 4202 1607 629 211 117 PfoI T'CCnGG_A Cuts at: 2742 2901 3287 3458 3678 2742 Size: 159 386 171 220 5830 Fragments arranged by size: 5830 386 220 171 159 PmeI GTTT'AAAC Cuts at: 4070 4070 Size: 6766 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4835 4867 6348 6380 4835 Size: 32 1481 32 5221 Fragments arranged by size: 5221 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2548 2746 2548 Size: 198 6568 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6766 PsiI TTA'TAA Cuts at: 4336 4336 Size: 6766 PspOMI G'GGCC_C Cuts at: 2398 2398 Size: 6766 PspXI vC'TCGA_Gb Cuts at: 3470 3470 Size: 6766 PstI C_TGCA'G Cuts at: 830 2028 2111 2224 3978 4118 830 Size: 1198 83 113 1754 140 3478 Fragments arranged by size: 3478 1754 1198 140 113 83 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6766 PvuII CAG'CTG Cuts at: 2557 2557 Size: 6766 SacI G_AGCT'C Cuts at: 2541 2541 Size: 6766 SalI G'TCGA_C Cuts at: 1936 4108 1936 Size: 2172 4594 SapI GCTCTTCn'nnn_ Cuts at: 2060 3998 2060 Size: 1938 4828 SbfI CC_TGCA'GG Cuts at: 4118 4118 Size: 6766 ScaI AGT'ACT Cuts at: 2798 5014 6726 2798 Size: 2216 1712 2838 Fragments arranged by size: 2838 2216 1712 SfcI C'TryA_G Cuts at: 826 1045 2024 2107 2220 3014 3974 4114 5249 Size: 219 979 83 113 794 960 140 1135 Cuts at: 5249 5930 6121 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 979 960 794 681 219 191 140 113 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6766 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6766 SmaI CCC'GGG Cuts at: 1872 3078 3983 4093 1872 Size: 1206 905 110 4545 Fragments arranged by size: 4545 1206 905 110 SmlI C'TyrA_G Cuts at: 820 1017 1943 2593 3470 3536 4870 5771 6033 Size: 197 926 650 877 66 1334 901 262 Cuts at: 6033 820 Size: 1553 Fragments arranged by size: 1553 1334 926 901 877 650 262 197 66 SnaBI TAC'GTA Cuts at: 493 4557 493 Size: 4064 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6766 SphI G_CATG'C Cuts at: 1132 4124 1132 Size: 2992 3774 SspI AAT'ATT Cuts at: 5 52 4690 5 Size: 47 4638 2081 Fragments arranged by size: 4638 2081 47 StuI AGG'CCT Cuts at: 1517 2591 1517 Size: 1074 5692 StyI C'CwwG_G Cuts at: 513 1065 2065 3549 3759 3841 3934 4205 513 Size: 552 1000 1484 210 82 93 271 3074 Fragments arranged by size: 3074 1484 1000 552 271 210 93 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2918 3087 3809 4793 4810 4963 5148 Size: 1255 1338 169 722 984 17 153 185 Cuts at: 5148 5593 325 Size: 445 1498 Fragments arranged by size: 1498 1338 1255 984 722 445 185 169 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2796 5012 6724 Size: 274 80 33 51 1443 819 2216 1712 Cuts at: 6724 96 Size: 138 Fragments arranged by size: 2216 1712 1443 819 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2219 2251 2744 2776 6726 6758 2219 Size: 32 493 32 3950 32 2227 Fragments arranged by size: 3950 2227 493 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6766 XbaI T'CTAG_A Cuts at: 4102 4102 Size: 6766 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2923 2923 Size: 6766 XhoI C'TCGA_G Cuts at: 1943 3470 1943 Size: 1527 5239 XmaI C'CCGG_G Cuts at: 1870 3076 3981 4091 1870 Size: 1206 905 110 4545 Fragments arranged by size: 4545 1206 905 110 XmnI GAAnn'nnTTC Cuts at: 2097 4895 2097 Size: 2798 3968 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4574 276 Size: 53 83 186 936 3040 2468 Fragments arranged by size: 3040 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI