(Circular) (Six-base) MAPSORT of: pFN21AB9513.seq Check: 6814 from: 1 to: 6145 pFN21AB9513 6145 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3955 278 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2102 3488 1937 Size: 165 1386 4594 Fragments arranged by size: 4594 1386 165 Acc65I G'GTAC_C Cuts at: 2304 3466 2304 Size: 1162 4983 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4208 5592 5860 1632 Size: 2576 1384 268 1917 Fragments arranged by size: 2576 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 2409 3381 5044 6021 1203 Size: 1206 972 1663 977 1327 Fragments arranged by size: 1663 1327 1206 977 972 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 2357 1166 Size: 1191 4954 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6145 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3243 3275 3243 Size: 32 6113 AlwNI CAG_nnn'CTG Cuts at: 2542 2764 3888 5460 2542 Size: 222 1124 1572 3227 Fragments arranged by size: 3227 1572 1124 222 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 3454 3680 3832 5941 1493 Size: 1961 226 152 2109 1697 Fragments arranged by size: 2109 1961 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 3470 1870 Size: 73 1527 4545 Fragments arranged by size: 4545 1527 73 BamHI G'GATC_C Cuts at: 2420 3475 3842 2420 Size: 1055 367 4723 Fragments arranged by size: 4723 1055 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2304 2703 3047 3466 4921 Size: 325 217 627 517 399 344 419 1455 Cuts at: 4921 618 Size: 1842 Fragments arranged by size: 1842 1455 627 517 419 399 344 325 217 BanII G_rGCy'C Cuts at: 1391 2169 2499 3117 3464 1391 Size: 778 330 618 347 4072 Fragments arranged by size: 4072 778 618 347 330 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2828 928 Size: 971 929 4245 Fragments arranged by size: 4245 971 929 BbvCI CC'TCA_GC Cuts at: 2069 2543 2069 Size: 474 5671 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4336 4370 1903 Size: 34 50 34 2315 34 3678 Fragments arranged by size: 3678 2315 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BfrBI ATG'CAT Cuts at: 3425 3425 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4756 136 Size: 107 122 71 631 605 3084 1525 Fragments arranged by size: 3084 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 3052 3347 4206 5362 1790 Size: 1262 295 859 1156 2573 Fragments arranged by size: 2573 1262 1156 859 295 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6145 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2628 3240 3876 Size: 416 53 83 399 24 1211 612 636 Cuts at: 3876 4834 442 Size: 958 1753 Fragments arranged by size: 1753 1211 958 636 612 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2837 4805 1239 Size: 1598 1968 2579 Fragments arranged by size: 2579 1968 1598 Bpu10I CC'TnA_GC Cuts at: 2069 2543 2069 Size: 474 5671 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4270 5135 5433 4270 Size: 865 298 4982 Fragments arranged by size: 4982 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2275 2606 4808 882 Size: 617 776 331 2202 2219 Fragments arranged by size: 2219 2202 776 617 331 BsaAI yAC'GTr Cuts at: 493 1204 3936 493 Size: 711 2732 2702 Fragments arranged by size: 2732 2702 711 BsaBI GATnn'nnATC Cuts at: 2553 6139 2553 Size: 3586 2559 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3279 3952 4334 4969 Size: 53 83 186 936 1746 673 382 635 Cuts at: 4969 275 Size: 1451 Fragments arranged by size: 1746 1451 936 673 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2423 3261 4578 4976 5250 5397 5808 2423 Size: 838 1317 398 274 147 411 2760 Fragments arranged by size: 2760 1317 838 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2556 2586 1178 Size: 30 165 30 1153 30 4737 Fragments arranged by size: 4737 1153 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6145 BseYI C'CCAG_C Cuts at: 2216 5348 5844 2216 Size: 3132 496 2517 Fragments arranged by size: 3132 2517 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2335 2537 1163 Size: 573 599 202 4771 Fragments arranged by size: 4771 599 573 202 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3079 3595 4356 5384 1540 Size: 275 186 1078 516 761 1028 2301 Fragments arranged by size: 2301 1078 1028 761 516 275 186 BsiHKAI G_wGCw'C Cuts at: 2499 2964 3117 4206 4291 5362 2499 Size: 465 153 1089 85 1071 3282 Fragments arranged by size: 3282 1089 1071 465 153 85 BsmI GAATG_Cn' Cuts at: 3656 3749 3656 Size: 93 6052 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2499 2964 3052 3117 3347 3464 Size: 399 379 330 465 88 65 230 117 Cuts at: 3464 4206 4291 5362 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 465 399 379 330 230 117 88 85 65 BspEI T'CCGG_A Cuts at: 2423 3261 5808 2423 Size: 838 2547 2760 Fragments arranged by size: 2760 2547 838 BspHI T'CATG_A Cuts at: 3136 4034 5764 3136 Size: 898 1730 3517 Fragments arranged by size: 3517 1730 898 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3500 844 Size: 2656 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4032 1394 Size: 429 2209 3507 Fragments arranged by size: 3507 2209 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4640 4814 65 Size: 1183 3392 174 1396 Fragments arranged by size: 3392 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4789 4976 1700 Size: 110 2979 187 2869 Fragments arranged by size: 2979 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6145 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 2524 794 Size: 1730 4415 BstBI TT'CG_AA Cuts at: 2436 4949 2436 Size: 2513 3632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstXI CCAn_nnnn'nTGG Cuts at: 2848 2848 Size: 6145 BstYI r'GATC_y Cuts at: 1972 2420 3475 3842 4227 4244 5696 5782 5794 Size: 448 1055 367 385 17 1452 86 12 Cuts at: 5794 6140 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1055 448 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2293 513 Size: 552 1228 4365 Fragments arranged by size: 4365 1228 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5406 Fragments arranged by size: 5406 683 56 BtsI GCAGTG_nn' Cuts at: 3409 3661 4455 4475 3409 Size: 252 794 20 5079 Fragments arranged by size: 5079 794 252 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 2838 3076 Size: 54 1425 50 275 18 393 615 238 Cuts at: 3076 3592 4481 6110 8 Size: 516 889 1629 43 Fragments arranged by size: 1629 1425 889 615 516 393 275 238 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3076 3592 1537 Size: 275 1264 516 4090 Fragments arranged by size: 4090 1264 516 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3105 4075 1353 Size: 1752 970 3423 Fragments arranged by size: 3423 1752 970 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4732 5106 5252 387 Size: 1064 138 3143 374 146 1280 Fragments arranged by size: 3143 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2497 3115 2497 Size: 618 5527 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2837 4208 4805 5592 5860 1239 Size: 393 1205 1371 597 787 268 1524 Fragments arranged by size: 1524 1371 1205 787 597 393 268 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 EcoRV GAT'ATC Cuts at: 2551 2551 Size: 6145 FspI TGC'GCA Cuts at: 4651 4651 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 5292 1678 Size: 3614 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4786 4818 4860 4892 1373 Size: 32 135 32 3214 32 42 32 2626 Fragments arranged by size: 3214 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2103 2953 3489 3735 669 Size: 988 281 165 850 536 246 3079 Fragments arranged by size: 3079 988 850 536 281 246 165 HindIII A'AGCT_T Cuts at: 748 2394 748 Size: 1646 4499 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KpnI G_GTAC'C Cuts at: 2308 3470 2308 Size: 1162 4983 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5259 5443 1305 Size: 3954 184 2007 Fragments arranged by size: 3954 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2840 10 Size: 54 1425 343 1008 3315 Fragments arranged by size: 3315 1425 1008 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 4103 4462 4621 518 Size: 648 1191 1746 359 159 2042 Fragments arranged by size: 2042 1746 1191 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2539 2815 3561 4238 5386 5631 1606 Size: 933 276 746 677 1148 245 2120 Fragments arranged by size: 2120 1148 933 746 677 276 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5593 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NsiI A_TGCA'T Cuts at: 3427 3427 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 2282 2413 3385 3429 3503 5048 1132 Size: 97 1053 131 972 44 74 1545 2229 Fragments arranged by size: 2229 1545 1053 972 131 97 74 44 PasI CC'CwG_GG Cuts at: 1348 3394 1348 Size: 2046 4099 PciI A'CATG_T Cuts at: 2409 3381 5044 2409 Size: 972 1663 3510 Fragments arranged by size: 3510 1663 972 PflMI CCAn_nnn'nTGG Cuts at: 1108 2586 2764 1108 Size: 1478 178 4489 Fragments arranged by size: 4489 1478 178 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PstI C_TGCA'G Cuts at: 830 2079 2596 3497 830 Size: 1249 517 901 3478 Fragments arranged by size: 3478 1249 901 517 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2539 2815 2539 Size: 276 5869 SacI G_AGCT'C Cuts at: 2499 3117 2499 Size: 618 5527 SalI G'TCGA_C Cuts at: 1936 2101 3487 1936 Size: 165 1386 4594 Fragments arranged by size: 4594 1386 165 SapI GCTCTTCn'nnn_ Cuts at: 3105 3105 Size: 6145 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SfcI C'TryA_G Cuts at: 826 1045 2075 2592 3493 4628 5309 5500 826 Size: 219 1030 517 901 1135 681 191 1471 Fragments arranged by size: 1471 1135 1030 901 681 517 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 3472 1872 Size: 1600 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4249 5150 5412 820 Size: 197 926 2306 901 262 1553 Fragments arranged by size: 2306 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3503 1132 Size: 2371 3774 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 3168 3194 3584 513 Size: 552 2103 26 390 3074 Fragments arranged by size: 3074 2103 552 390 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2397 2490 3035 3386 4172 4189 4342 Size: 1255 817 93 545 351 786 17 153 Cuts at: 4342 4527 4972 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 817 786 545 445 351 185 153 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 3024 4391 Size: 274 80 33 51 1443 225 822 1367 Cuts at: 4391 6103 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1367 822 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3073 3105 6105 6137 3073 Size: 32 3000 32 3081 Fragments arranged by size: 3081 3000 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2679 2679 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 3470 1870 Size: 1600 4545 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3953 276 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI