Restriction map for pFN21AB9513
 (Circular) (Six-base) MAPSORT of: pFN21AB9513.seq  Check: 6814  from: 1  to: 6145



pFN21AB9513  6145 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3955     278 
   Size:         53      83     186     936    2419    2468

  Fragments arranged by size: 

                2468    2419     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2102    3488    1937 
   Size:        165    1386    4594

  Fragments arranged by size: 

                4594    1386     165
 
Acc65I G'GTAC_C

Cuts at:   2304    3466    2304 
   Size:       1162    4983
 
AclI AA'CG_TT

Cuts at:   1563    4272    4645    1563 
   Size:       2709     373    3063

  Fragments arranged by size: 

                3063    2709     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    4208    5592    5860    1632 
   Size:       2576    1384     268    1917

  Fragments arranged by size: 

                2576    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5948
 
AflIII A'CryG_T

Cuts at:   1203    2409    3381    5044    6021    1203 
   Size:       1206     972    1663     977    1327

  Fragments arranged by size: 

                1663    1327    1206     977     972
 
AgeI A'CCGG_T

Cuts at:   4976    4976 
   Size:       6145
 
AhdI GACnn_n'nnGTC

Cuts at:   4874    4874 
   Size:       6145
 
AleI CACnn'nnGTG

Cuts at:   1166    2357    1166 
   Size:       1191    4954
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6145
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3243    3275    3243 
   Size:         32    6113
 
AlwNI CAG_nnn'CTG

Cuts at:   2542    2764    3888    5460    2542 
   Size:        222    1124    1572    3227

  Fragments arranged by size: 

                3227    1572    1124     222
 
ApaLI G'TGCA_C

Cuts at:   4202    5358    4202 
   Size:       1156    4989
 
ApoI r'AATT_y

Cuts at:   1493    3454    3680    3832    5941    1493 
   Size:       1961     226     152    2109    1697

  Fragments arranged by size: 

                2109    1961    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4699     160 
   Size:        872    3667    1606

  Fragments arranged by size: 

                3667    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6145
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3470    1870 
   Size:         73    1527    4545

  Fragments arranged by size: 

                4545    1527      73
 
BamHI G'GATC_C

Cuts at:   2420    3475    3842    2420 
   Size:       1055     367    4723

  Fragments arranged by size: 

                4723    1055     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2304    2703    3047    3466    4921 
   Size:        325     217     627     517     399     344     419    1455

Cuts at:   4921     618 
   Size:       1842

  Fragments arranged by size: 

                1842    1455     627     517     419     399     344     325
                 217
 
BanII G_rGCy'C

Cuts at:   1391    2169    2499    3117    3464    1391 
   Size:        778     330     618     347    4072

  Fragments arranged by size: 

                4072     778     618     347     330
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2828     928 
   Size:        971     929    4245

  Fragments arranged by size: 

                4245     971     929
 
BbvCI CC'TCA_GC

Cuts at:   2069    2543    2069 
   Size:        474    5671
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4336    4370    1903 
   Size:         34      50      34    2315      34    3678

  Fragments arranged by size: 

                3678    2315      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4037    5247    4037 
   Size:       1210    4935
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6145
 
BfrBI ATG'CAT

Cuts at:   3425    3425 
   Size:       6145
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4756     136 
   Size:        107     122      71     631     605    3084    1525

  Fragments arranged by size: 

                3084    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6140    6140 
   Size:       6145
 
BlpI GC'TnA_GC

Cuts at:   3562    3562 
   Size:       6145
 
Bme1580I G_kGCm'C

Cuts at:   1790    3052    3347    4206    5362    1790 
   Size:       1262     295     859    1156    2573

  Fragments arranged by size: 

                2573    1262    1156     859     295
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6145
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2628    3240    3876 
   Size:        416      53      83     399      24    1211     612     636

Cuts at:   3876    4834     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1211     958     636     612     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6145
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2837    4805    1239 
   Size:       1598    1968    2579

  Fragments arranged by size: 

                2579    1968    1598
 
Bpu10I CC'TnA_GC

Cuts at:   2069    2543    2069 
   Size:        474    5671
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4270    5135    5433    4270 
   Size:        865     298    4982

  Fragments arranged by size: 

                4982     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2275    2606    4808     882 
   Size:        617     776     331    2202    2219

  Fragments arranged by size: 

                2219    2202     776     617     331
 
BsaAI yAC'GTr

Cuts at:    493    1204    3936     493 
   Size:        711    2732    2702

  Fragments arranged by size: 

                2732    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   2553    6139    2553 
   Size:       3586    2559
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3279    3952    4334    4969 
   Size:         53      83     186     936    1746     673     382     635

Cuts at:   4969     275 
   Size:       1451

  Fragments arranged by size: 

                1746    1451     936     673     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2423    3261    4578    4976    5250    5397    5808    2423 
   Size:        838    1317     398     274     147     411    2760

  Fragments arranged by size: 

                2760    1317     838     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2556    2586    1178 
   Size:         30     165      30    1153      30    4737

  Fragments arranged by size: 

                4737    1153     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       6145
 
BseYI C'CCAG_C

Cuts at:   2216    5348    5844    2216 
   Size:       3132     496    2517

  Fragments arranged by size: 

                3132    2517     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2335    2537    1163 
   Size:        573     599     202    4771

  Fragments arranged by size: 

                4771     599     573     202
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3079    3595    4356    5384    1540 
   Size:        275     186    1078     516     761    1028    2301

  Fragments arranged by size: 

                2301    1078    1028     761     516     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2499    2964    3117    4206    4291    5362    2499 
   Size:        465     153    1089      85    1071    3282

  Fragments arranged by size: 

                3282    1089    1071     465     153      85
 
BsmI GAATG_Cn'

Cuts at:   3656    3749    3656 
   Size:         93    6052
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2169    2499    2964    3052    3117    3347    3464 
   Size:        399     379     330     465      88      65     230     117

Cuts at:   3464    4206    4291    5362    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     465     399     379     330     230
                 117      88      85      65
 
BspEI T'CCGG_A

Cuts at:   2423    3261    5808    2423 
   Size:        838    2547    2760

  Fragments arranged by size: 

                2760    2547     838
 
BspHI T'CATG_A

Cuts at:   3136    4034    5764    3136 
   Size:        898    1730    3517

  Fragments arranged by size: 

                3517    1730     898
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3500     844 
   Size:       2656    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4032    1394 
   Size:        429    2209    3507

  Fragments arranged by size: 

                3507    2209     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4640    4814      65 
   Size:       1183    3392     174    1396

  Fragments arranged by size: 

                3392    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4789    4976    1700 
   Size:        110    2979     187    2869

  Fragments arranged by size: 

                2979    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6145
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6145
 
BssSI C'ACGA_G

Cuts at:   4205    5217    4205 
   Size:       1012    5133
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2524     794 
   Size:       1730    4415
 
BstBI TT'CG_AA

Cuts at:   2436    4949    2436 
   Size:       2513    3632
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6145
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2848    2848 
   Size:       6145
 
BstYI r'GATC_y

Cuts at:   1972    2420    3475    3842    4227    4244    5696    5782    5794 
   Size:        448    1055     367     385      17    1452      86      12

Cuts at:   5794    6140    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452    1055     448     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2293     513 
   Size:        552    1228    4365

  Fragments arranged by size: 

                4365    1228     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5406

  Fragments arranged by size: 

                5406     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3409    3661    4455    4475    3409 
   Size:        252     794      20    5079

  Fragments arranged by size: 

                5079     794     252      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6145
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6110
 
DraI TTT'AAA

Cuts at:   3449    3449 
   Size:       6145
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5152     809 
   Size:        507    3836    1802

  Fragments arranged by size: 

                3836    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2223    2838    3076 
   Size:         54    1425      50     275      18     393     615     238

Cuts at:   3076    3592    4481    6110       8 
   Size:        516     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     615     516     393     275     238
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3076    3592    1537 
   Size:        275    1264     516    4090

  Fragments arranged by size: 

                4090    1264     516     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3105    4075    1353 
   Size:       1752     970    3423

  Fragments arranged by size: 

                3423    1752     970
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4732    5106    5252     387 
   Size:       1064     138    3143     374     146    1280

  Fragments arranged by size: 

                3143    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2497    3115    2497 
   Size:        618    5527
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2837    4208    4805    5592    5860    1239 
   Size:        393    1205    1371     597     787     268    1524

  Fragments arranged by size: 

                1524    1371    1205     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   3454    3832    3454 
   Size:        378    5767
 
EcoRV GAT'ATC

Cuts at:   2551    2551 
   Size:       6145
 
FspI TGC'GCA

Cuts at:   4651    4651 
   Size:       6145
 
HaeII r_GCGC'y

Cuts at:   1678    5292    1678 
   Size:       3614    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4786    4818    4860    4892    1373 
   Size:         32     135      32    3214      32      42      32    2626

  Fragments arranged by size: 

                3214    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2103    2953    3489    3735     669 
   Size:        988     281     165     850     536     246    3079

  Fragments arranged by size: 

                3079     988     850     536     281     246     165
 
HindIII A'AGCT_T

Cuts at:    748    2394     748 
   Size:       1646    4499
 
HpaI GTT'AAC

Cuts at:   3735    3735 
   Size:       6145
 
KpnI G_GTAC'C

Cuts at:   2308    3470    2308 
   Size:       1162    4983
 
MfeI C'AATT_G

Cuts at:   3744    3744 
   Size:       6145
 
MluI A'CGCG_T

Cuts at:   6021    6021 
   Size:       6145
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5259    5443    1305 
   Size:       3954     184    2007

  Fragments arranged by size: 

                3954    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2840      10 
   Size:         54    1425     343    1008    3315

  Fragments arranged by size: 

                3315    1425    1008     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2357    4103    4462    4621     518 
   Size:        648    1191    1746     359     159    2042

  Fragments arranged by size: 

                2042    1746    1191     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2539    2815    3561    4238    5386    5631    1606 
   Size:        933     276     746     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     933     746     677     276     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5593
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6145
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6145
 
NotI GC'GGCC_GC

Cuts at:   3592    3592 
   Size:       6145
 
NsiI A_TGCA'T

Cuts at:   3427    3427 
   Size:       6145
 
NspI r_CATG'y

Cuts at:   1132    1229    2282    2413    3385    3429    3503    5048    1132 
   Size:         97    1053     131     972      44      74    1545    2229

  Fragments arranged by size: 

                2229    1545    1053     972     131      97      74      44
            
 
PasI CC'CwG_GG

Cuts at:   1348    3394    1348 
   Size:       2046    4099
 
PciI A'CATG_T

Cuts at:   2409    3381    5044    2409 
   Size:        972    1663    3510

  Fragments arranged by size: 

                3510    1663     972
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2586    2764    1108 
   Size:       1478     178    4489

  Fragments arranged by size: 

                4489    1478     178
 
PmeI GTTT'AAAC

Cuts at:   3449    3449 
   Size:       6145
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4214    4246    5727    5759    4214 
   Size:         32    1481      32    4600

  Fragments arranged by size: 

                4600    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6145
 
PsiI TTA'TAA

Cuts at:   3715    3715 
   Size:       6145
 
PstI C_TGCA'G

Cuts at:    830    2079    2596    3497     830 
   Size:       1249     517     901    3478

  Fragments arranged by size: 

                3478    1249     901     517
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6145
 
PvuII CAG'CTG

Cuts at:   2539    2815    2539 
   Size:        276    5869
 
SacI G_AGCT'C

Cuts at:   2499    3117    2499 
   Size:        618    5527
 
SalI G'TCGA_C

Cuts at:   1936    2101    3487    1936 
   Size:        165    1386    4594

  Fragments arranged by size: 

                4594    1386     165
 
SapI GCTCTTCn'nnn_

Cuts at:   3105    3105 
   Size:       6145
 
SbfI CC_TGCA'GG

Cuts at:   3497    3497 
   Size:       6145
 
ScaI AGT'ACT

Cuts at:   4393    6105    4393 
   Size:       1712    4433
 
SfcI C'TryA_G

Cuts at:    826    1045    2075    2592    3493    4628    5309    5500     826 
   Size:        219    1030     517     901    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1030     901     681     517     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6145
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6145
 
SmaI CCC'GGG

Cuts at:   1872    3472    1872 
   Size:       1600    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4249    5150    5412     820 
   Size:        197     926    2306     901     262    1553

  Fragments arranged by size: 

                2306    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3936     493 
   Size:       3443    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6145
 
SphI G_CATG'C

Cuts at:   1132    3503    1132 
   Size:       2371    3774
 
SspI AAT'ATT

Cuts at:      5      52    4069       5 
   Size:         47    4017    2081

  Fragments arranged by size: 

                4017    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6145
 
StyI C'CwwG_G

Cuts at:    513    1065    3168    3194    3584     513 
   Size:        552    2103      26     390    3074

  Fragments arranged by size: 

                3074    2103     552     390      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2397    2490    3035    3386    4172    4189    4342 
   Size:       1255     817      93     545     351     786      17     153

Cuts at:   4342    4527    4972     325 
   Size:        185     445    1498

  Fragments arranged by size: 

                1498    1255     817     786     545     445     351     185
                 153      93      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2202    3024    4391 
   Size:        274      80      33      51    1443     225     822    1367

Cuts at:   4391    6103      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1367     822     274     225     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3073    3105    6105    6137    3073 
   Size:         32    3000      32    3081

  Fragments arranged by size: 

                3081    3000      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6145
 
XbaI T'CTAG_A

Cuts at:   3481    3481 
   Size:       6145
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2679    2679 
   Size:       6145
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6145
 
XmaI C'CCGG_G

Cuts at:   1870    3470    1870 
   Size:       1600    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4274    4274 
   Size:       6145
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3953     276 
   Size:         53      83     186     936    2419    2468

  Fragments arranged by size: 

                2468    2419     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NsiI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI    PvuII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI     BplI
    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII    EcoNI EcoO109I     FalI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     PacI
     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    RsrII    SacII
    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI
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