(Circular) (Six-base) MAPSORT of: pFN21AB9506.seq Check: 1770 from: 1 to: 6031 pFN21AB9506 6031 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2344 2344 Size: 6031 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3841 278 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3374 1937 Size: 1437 4594 Acc65I G'GTAC_C Cuts at: 3352 3352 Size: 6031 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2284 4094 5478 5746 1632 Size: 652 1810 1384 268 1917 Fragments arranged by size: 1917 1810 1384 652 268 AfeI AGC'GCT Cuts at: 2150 2150 Size: 6031 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5834 AflIII A'CryG_T Cuts at: 1203 4930 5907 1203 Size: 3727 977 1327 Fragments arranged by size: 3727 1327 977 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 4760 4760 Size: 6031 AleI CACnn'nnGTG Cuts at: 1166 2864 1166 Size: 1698 4333 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6031 AlwNI CAG_nnn'CTG Cuts at: 2543 3774 5346 2543 Size: 1231 1572 3228 Fragments arranged by size: 3228 1572 1231 ApaI G_GGCC'C Cuts at: 2079 2086 2146 2943 3077 2079 Size: 7 60 797 134 5033 Fragments arranged by size: 5033 797 134 60 7 ApaLI G'TGCA_C Cuts at: 4088 5244 4088 Size: 1156 4875 ApoI r'AATT_y Cuts at: 1493 3340 3566 3718 5827 1493 Size: 1847 226 152 2109 1697 Fragments arranged by size: 2109 1847 1697 226 152 AscI GG'CGCG_CC Cuts at: 2131 2131 Size: 6031 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2242 2254 2684 3070 3103 3356 Size: 73 135 164 12 430 386 33 253 Cuts at: 3356 1870 Size: 4545 Fragments arranged by size: 4545 430 386 253 164 135 73 33 12 BamHI G'GATC_C Cuts at: 3361 3728 3361 Size: 367 5664 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2689 3062 3191 3352 Size: 325 217 627 264 638 373 129 161 Cuts at: 3352 4807 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 638 627 373 325 264 217 161 129 BanII G_rGCy'C Cuts at: 1391 2079 2086 2146 2195 2255 2391 2460 2779 Size: 688 7 60 49 60 136 69 319 Cuts at: 2779 2943 3077 3350 1391 Size: 164 134 273 4072 Fragments arranged by size: 4072 688 319 273 164 136 134 69 60 60 49 7 BbeI G_GCGC'C Cuts at: 2055 2693 3195 2055 Size: 638 502 4891 Fragments arranged by size: 4891 638 502 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5060 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4222 4256 1903 Size: 34 2285 34 3678 Fragments arranged by size: 3678 2285 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3149 3923 5133 3149 Size: 774 1210 4047 Fragments arranged by size: 4047 1210 774 BclI T'GATC_A Cuts at: 1548 1548 Size: 6031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2045 2141 2552 Size: 107 122 71 631 605 373 96 411 Cuts at: 2552 4642 136 Size: 2090 1525 Fragments arranged by size: 2090 1525 631 605 411 373 122 107 96 71 BglII A'GATC_T Cuts at: 6026 6026 Size: 6031 BlpI GC'TnA_GC Cuts at: 3112 3120 3448 3112 Size: 8 328 5695 Fragments arranged by size: 5695 328 8 Bme1580I G_kGCm'C Cuts at: 1790 2079 2086 2146 2375 2943 3067 3077 4092 Size: 289 7 60 229 568 124 10 1015 Cuts at: 4092 5248 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1015 568 289 229 124 60 10 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6031 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2937 3762 4720 Size: 416 53 83 399 24 1520 825 958 Cuts at: 4720 442 Size: 1753 Fragments arranged by size: 1753 1520 958 825 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6031 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2410 2710 2410 Size: 300 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2432 2868 3164 3287 4691 1239 Size: 968 225 436 296 123 1404 2579 Fragments arranged by size: 2579 1404 968 436 296 225 123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4156 5021 5319 4156 Size: 865 298 4868 Fragments arranged by size: 4868 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2167 2882 2966 4694 882 Size: 617 668 715 84 1728 2219 Fragments arranged by size: 2219 1728 715 668 617 84 BsaAI yAC'GTr Cuts at: 493 1204 3822 493 Size: 711 2618 2702 Fragments arranged by size: 2702 2618 711 BsaBI GATnn'nnATC Cuts at: 6025 6025 Size: 6031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 2690 3192 3838 Size: 53 83 186 936 519 638 502 646 Cuts at: 3838 4220 4855 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 646 638 635 519 502 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2926 4464 4862 5136 5283 5694 2926 Size: 1538 398 274 147 411 3263 Fragments arranged by size: 3263 1538 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2866 2896 1178 Size: 30 165 30 1463 30 4313 Fragments arranged by size: 4313 1463 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2836 3332 1186 Size: 1650 496 3885 Fragments arranged by size: 3885 1650 496 BseYI C'CCAG_C Cuts at: 2145 2459 2567 5234 5730 2145 Size: 314 108 2667 496 2446 Fragments arranged by size: 2667 2446 496 314 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5458 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2073 3481 4242 5270 1540 Size: 275 186 72 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 72 BsiHKAI G_wGCw'C Cuts at: 2391 2828 3162 4092 4177 5248 2391 Size: 437 334 930 85 1071 3174 Fragments arranged by size: 3174 1071 930 437 334 85 BsiWI C'GTAC_G Cuts at: 2626 2626 Size: 6031 BsmI GAATG_Cn' Cuts at: 3542 3635 3542 Size: 93 5938 BsmBI CGTCTCn'nnnn_ Cuts at: 2485 2485 Size: 6031 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2086 2146 2195 2255 2375 2391 Size: 399 289 7 60 49 60 120 16 Cuts at: 2391 2460 2779 2828 2943 3067 3077 3162 3350 Size: 69 319 49 115 124 10 85 188 Cuts at: 3350 4092 4177 5248 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 319 289 188 124 120 115 85 85 69 60 60 49 49 16 10 7 BspEI T'CCGG_A Cuts at: 5694 5694 Size: 6031 BspHI T'CATG_A Cuts at: 3920 5650 3920 Size: 1730 4301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2344 3386 844 Size: 1500 1042 3489 Fragments arranged by size: 3489 1500 1042 BsrBI CCG'CTC Cuts at: 1394 1823 3148 3918 1394 Size: 429 1325 770 3507 Fragments arranged by size: 3507 1325 770 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4526 4700 65 Size: 1183 3278 174 1396 Fragments arranged by size: 3278 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 2788 4675 4862 1700 Size: 110 238 740 1887 187 2869 Fragments arranged by size: 2869 1887 740 238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6031 BssHII G'CGCG_C Cuts at: 1925 2131 1925 Size: 206 5825 BssSI C'ACGA_G Cuts at: 4091 5103 4091 Size: 1012 5019 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6031 BstBI TT'CG_AA Cuts at: 4835 4835 Size: 6031 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6031 BstXI CCAn_nnnn'nTGG Cuts at: 2431 2491 2866 3001 2431 Size: 60 375 135 5461 Fragments arranged by size: 5461 375 135 60 BstYI r'GATC_y Cuts at: 1972 3361 3728 4113 4130 5582 5668 5680 6026 Size: 1389 367 385 17 1452 86 12 346 Cuts at: 6026 1972 Size: 1977 Fragments arranged by size: 1977 1452 1389 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2041 2067 2086 2136 2178 2490 2706 Size: 552 976 26 19 50 42 312 216 Cuts at: 2706 513 Size: 3838 Fragments arranged by size: 3838 976 552 312 216 50 42 26 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5292 Fragments arranged by size: 5292 683 56 BtsI GCAGTG_nn' Cuts at: 2164 2499 2601 2844 3050 3218 3547 4341 4361 Size: 335 102 243 206 168 329 794 20 Cuts at: 4361 2164 Size: 3834 Fragments arranged by size: 3834 794 335 329 243 206 168 102 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5996 DraI TTT'AAA Cuts at: 3335 3335 Size: 6031 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5038 809 Size: 507 3722 1802 Fragments arranged by size: 3722 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2532 2553 Size: 54 1425 50 275 18 240 462 21 Cuts at: 2553 2595 2768 2790 3024 3478 4367 5996 8 Size: 42 173 22 234 454 889 1629 43 Fragments arranged by size: 1629 1425 889 462 454 275 240 234 173 54 50 43 42 22 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2070 3478 1537 Size: 275 258 1408 4090 Fragments arranged by size: 4090 1408 275 258 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 2448 2964 3128 3961 1353 Size: 1026 69 516 164 833 3423 Fragments arranged by size: 3423 1026 833 516 164 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4618 4992 5138 387 Size: 1064 138 3029 374 146 1280 Fragments arranged by size: 3029 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2389 2389 Size: 6031 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2284 2432 2868 3164 3287 4094 Size: 393 575 77 148 436 296 123 807 Cuts at: 4094 4691 5478 5746 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 807 787 597 575 436 393 296 268 148 123 77 EcoO109I rG'GnC_Cy Cuts at: 2082 2142 2521 2731 2939 3074 2082 Size: 60 379 210 208 135 5039 Fragments arranged by size: 5039 379 210 208 135 60 EcoRI G'AATT_C Cuts at: 3340 3718 3340 Size: 378 5653 FseI GG_CCGG'CC Cuts at: 2792 2792 Size: 6031 FspI TGC'GCA Cuts at: 4537 4537 Size: 6031 HaeII r_GCGC'y Cuts at: 1678 2055 2152 2404 2693 3195 5178 1678 Size: 377 97 252 289 502 1983 2531 Fragments arranged by size: 2531 1983 502 377 289 252 97 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2225 2257 2258 Size: 32 135 32 620 32 1 32 1 Cuts at: 2258 2290 2866 2898 4672 4704 4746 4778 1373 Size: 32 576 32 1774 32 42 32 2626 Fragments arranged by size: 2626 1774 620 576 135 42 32 32 32 32 32 32 32 32 1 1 HincII GTy'rAC Cuts at: 669 1657 1938 3375 3621 669 Size: 988 281 1437 246 3079 Fragments arranged by size: 3079 1437 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6031 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KasI G'GCGC_C Cuts at: 2051 2689 3191 2051 Size: 638 502 4891 Fragments arranged by size: 4891 638 502 KpnI G_GTAC'C Cuts at: 3356 3356 Size: 6031 MfeI C'AATT_G Cuts at: 3630 3630 Size: 6031 MluI A'CGCG_T Cuts at: 5907 5907 Size: 6031 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5145 5329 1305 Size: 3840 184 2007 Fragments arranged by size: 3840 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2534 2555 10 Size: 54 1425 343 702 21 3486 Fragments arranged by size: 3486 1425 702 343 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2489 2864 3989 4348 4507 518 Size: 648 1323 375 1125 359 159 2042 Fragments arranged by size: 2042 1323 1125 648 375 359 159 MspA1I CmG'CkG Cuts at: 1606 2069 2088 2107 2138 3116 3124 3447 4124 Size: 463 19 19 31 978 8 323 677 Cuts at: 4124 5272 5517 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 978 677 463 323 245 31 19 19 8 NaeI GCC'GGC Cuts at: 2050 2790 2050 Size: 740 5291 NarI GG'CG_CC Cuts at: 2052 2690 3192 2052 Size: 638 502 4891 Fragments arranged by size: 4891 638 502 NcoI C'CATG_G Cuts at: 513 1065 2490 513 Size: 552 1425 4054 Fragments arranged by size: 4054 1425 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6031 NgoMIV G'CCGG_C Cuts at: 2048 2788 2048 Size: 740 5291 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6031 NotI GC'GGCC_GC Cuts at: 3478 3478 Size: 6031 NspI r_CATG'y Cuts at: 1132 1229 3389 4934 1132 Size: 97 2160 1545 2229 Fragments arranged by size: 2229 2160 1545 97 PasI CC'CwG_GG Cuts at: 1348 2836 1348 Size: 1488 4543 PciI A'CATG_T Cuts at: 4930 4930 Size: 6031 PflMI CCAn_nnn'nTGG Cuts at: 1108 2368 1108 Size: 1260 4771 PfoI T'CCnGG_A Cuts at: 2448 2907 3084 3141 2448 Size: 459 177 57 5338 Fragments arranged by size: 5338 459 177 57 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4100 4132 5613 5645 4100 Size: 32 1481 32 4486 Fragments arranged by size: 4486 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2521 2521 Size: 6031 PshAI GACnn'nnGTC Cuts at: 1613 2444 1613 Size: 831 5200 PsiI TTA'TAA Cuts at: 2612 3601 2612 Size: 989 5042 PspOMI G'GGCC_C Cuts at: 2075 2082 2142 2939 3073 2075 Size: 7 60 797 134 5033 Fragments arranged by size: 5033 797 134 60 7 PstI C_TGCA'G Cuts at: 830 2581 3383 830 Size: 1751 802 3478 Fragments arranged by size: 3478 1751 802 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6031 PvuII CAG'CTG Cuts at: 3116 3124 3116 Size: 8 6023 SacI G_AGCT'C Cuts at: 2391 2391 Size: 6031 SacII CC_GC'GG Cuts at: 2070 2089 2139 2070 Size: 19 50 5962 Fragments arranged by size: 5962 50 19 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SanDI GG'GwC_CC Cuts at: 2521 2521 Size: 6031 SapI GCTCTTCn'nnn_ Cuts at: 2379 2448 2379 Size: 69 5962 SbfI CC_TGCA'GG Cuts at: 3383 3383 Size: 6031 ScaI AGT'ACT Cuts at: 4279 5991 4279 Size: 1712 4319 SfcI C'TryA_G Cuts at: 826 1045 2577 3379 4514 5195 5386 826 Size: 219 1532 802 1135 681 191 1471 Fragments arranged by size: 1532 1471 1135 802 681 219 191 SfoI GGC'GCC Cuts at: 2053 2691 3193 2053 Size: 638 502 4891 Fragments arranged by size: 4891 638 502 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6031 SmaI CCC'GGG Cuts at: 1872 2080 3072 3358 1872 Size: 208 992 286 4545 Fragments arranged by size: 4545 992 286 208 SmlI C'TyrA_G Cuts at: 820 1017 1943 4135 5036 5298 820 Size: 197 926 2192 901 262 1553 Fragments arranged by size: 2192 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3822 493 Size: 3329 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6031 SphI G_CATG'C Cuts at: 1132 3389 1132 Size: 2257 3774 SrfI GCCC'GGGC Cuts at: 3072 3072 Size: 6031 SspI AAT'ATT Cuts at: 5 52 3955 5 Size: 47 3903 2081 Fragments arranged by size: 3903 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6031 StyI C'CwwG_G Cuts at: 513 1065 2490 3027 3057 3470 513 Size: 552 1425 537 30 413 3074 Fragments arranged by size: 3074 1425 552 537 413 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2755 2813 4058 4075 4228 4413 4858 Size: 1255 1175 58 1245 17 153 185 445 Cuts at: 4858 325 Size: 1498 Fragments arranged by size: 1498 1255 1245 1175 445 185 153 58 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4277 5989 96 Size: 274 80 33 51 1443 2300 1712 138 Fragments arranged by size: 2300 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5991 6023 5991 Size: 32 5999 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6031 XbaI T'CTAG_A Cuts at: 3367 3367 Size: 6031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2428 2431 2428 Size: 3 6028 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 2078 3070 3356 1870 Size: 208 992 286 4545 Fragments arranged by size: 4545 992 286 208 XmnI GAAnn'nnTTC Cuts at: 4160 4160 Size: 6031 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3839 276 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BfrBI Bpu10I BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SwaI