(Circular) (Six-base) MAPSORT of: pFN21AB9504.seq Check: 9985 from: 1 to: 8647 pFN21AB9504 8647 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3846 5231 3846 Size: 1385 7262 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6457 278 Size: 53 83 186 936 4921 2468 Fragments arranged by size: 4921 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4491 5990 1937 Size: 2554 1499 4594 Fragments arranged by size: 4594 2554 1499 Acc65I G'GTAC_C Cuts at: 4944 5968 4944 Size: 1024 7623 AclI AA'CG_TT Cuts at: 1563 6774 7147 1563 Size: 5211 373 3063 Fragments arranged by size: 5211 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3548 3730 6710 8094 8362 1632 Size: 1916 182 2980 1384 268 1917 Fragments arranged by size: 2980 1917 1916 1384 268 182 AfeI AGC'GCT Cuts at: 5630 5630 Size: 8647 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8450 AflIII A'CryG_T Cuts at: 1203 4791 7546 8523 1203 Size: 3588 2755 977 1327 Fragments arranged by size: 3588 2755 1327 977 AgeI A'CCGG_T Cuts at: 7478 7478 Size: 8647 AhdI GACnn_n'nnGTC Cuts at: 7376 7376 Size: 8647 AleI CACnn'nnGTG Cuts at: 1166 2390 5276 5522 5888 1166 Size: 1224 2886 246 366 3925 Fragments arranged by size: 3925 2886 1224 366 246 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2552 3281 3623 5489 5557 5624 5664 1184 Size: 1368 729 342 1866 68 67 40 4167 Fragments arranged by size: 4167 1866 1368 729 342 68 67 40 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3501 3533 3501 Size: 32 8615 AlwNI CAG_nnn'CTG Cuts at: 2066 2194 3088 3526 3817 3979 4070 4176 4300 Size: 128 894 438 291 162 91 106 124 Cuts at: 4300 4780 5359 5482 5597 5618 5695 5732 5848 Size: 480 579 123 115 21 77 37 116 Cuts at: 5848 6390 7962 2066 Size: 542 1572 2751 Fragments arranged by size: 2751 1572 894 579 542 480 438 291 162 128 124 123 116 115 106 91 77 37 21 ApaI G_GGCC'C Cuts at: 4897 4897 Size: 8647 ApaLI G'TGCA_C Cuts at: 2458 3077 4655 6704 7860 2458 Size: 619 1578 2049 1156 3245 Fragments arranged by size: 3245 2049 1578 1156 619 ApoI r'AATT_y Cuts at: 1493 5132 5956 6182 6334 8443 1493 Size: 3639 824 226 152 2109 1697 Fragments arranged by size: 3639 2109 1697 824 226 152 AseI AT'TA_AT Cuts at: 160 1032 7201 160 Size: 872 6169 1606 Fragments arranged by size: 6169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8647 AvaI C'yCGr_G Cuts at: 1870 1943 4208 4592 4832 5972 1870 Size: 73 2265 384 240 1140 4545 Fragments arranged by size: 4545 2265 1140 384 240 73 BamHI G'GATC_C Cuts at: 4908 5977 6344 4908 Size: 1069 367 7211 Fragments arranged by size: 7211 1069 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2369 4380 4870 4944 5080 Size: 325 217 627 582 2011 490 74 136 Cuts at: 5080 5278 5968 7423 618 Size: 198 690 1455 1842 Fragments arranged by size: 2011 1842 1455 690 627 582 490 325 217 198 136 74 BanII G_rGCy'C Cuts at: 1391 2442 2912 3326 3360 3903 4674 4897 5589 Size: 1051 470 414 34 543 771 223 692 Cuts at: 5589 5966 1391 Size: 377 4072 Fragments arranged by size: 4072 1051 771 692 543 470 414 377 223 34 BbeI G_GCGC'C Cuts at: 4874 4874 Size: 8647 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3298 3769 3851 928 Size: 971 1399 471 82 5724 Fragments arranged by size: 5724 1399 971 471 82 BbvCI CC'TCA_GC Cuts at: 4058 4283 5938 4058 Size: 225 1655 6767 Fragments arranged by size: 6767 1655 225 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4544 4578 6838 6872 1903 Size: 34 2607 34 2260 34 3678 Fragments arranged by size: 3678 2607 2260 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6539 7749 6539 Size: 1210 7437 BclI T'GATC_A Cuts at: 1548 1548 Size: 8647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3822 4018 7258 Size: 107 122 71 631 605 2150 196 3240 Cuts at: 7258 136 Size: 1525 Fragments arranged by size: 3240 2150 1525 631 605 196 122 107 71 BglII A'GATC_T Cuts at: 8642 8642 Size: 8647 BlpI GC'TnA_GC Cuts at: 3269 4133 5169 6064 3269 Size: 864 1036 895 5852 Fragments arranged by size: 5852 1036 895 864 Bme1580I G_kGCm'C Cuts at: 1790 2462 2663 2830 3053 3081 4385 4659 4751 Size: 672 201 167 223 28 1304 274 92 Cuts at: 4751 4897 6708 7864 1790 Size: 146 1811 1156 2573 Fragments arranged by size: 2573 1811 1304 1156 672 274 223 201 167 146 92 28 BmgBI CAC'GTC Cuts at: 1318 3289 1318 Size: 1971 6676 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2666 2768 5128 Size: 416 53 83 399 24 1249 102 2360 Cuts at: 5128 5914 6378 7336 442 Size: 786 464 958 1753 Fragments arranged by size: 2360 1753 1249 958 786 464 416 399 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3373 3887 3917 4945 4994 5198 7307 1239 Size: 2134 514 30 1028 49 204 2109 2579 Fragments arranged by size: 2579 2134 2109 1028 514 204 49 30 Bpu10I CC'TnA_GC Cuts at: 3482 4058 4218 4283 4457 4604 5938 3482 Size: 576 160 65 174 147 1334 6191 Fragments arranged by size: 6191 1334 576 174 160 147 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3122 4619 5006 5192 6772 7637 7935 3122 Size: 1497 387 186 1580 865 298 3834 Fragments arranged by size: 3834 1580 1497 865 387 298 186 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3422 4578 4682 7310 882 Size: 617 1923 1156 104 2628 2219 Fragments arranged by size: 2628 2219 1923 1156 617 104 BsaAI yAC'GTr Cuts at: 493 1204 4792 6438 493 Size: 711 3588 1646 2702 Fragments arranged by size: 3588 2702 1646 711 BsaBI GATnn'nnATC Cuts at: 5267 8641 5267 Size: 3374 5273 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4871 4992 5332 6454 Size: 53 83 186 936 3338 121 340 1122 Cuts at: 6454 6836 7471 275 Size: 382 635 1451 Fragments arranged by size: 3338 1451 1122 936 635 382 340 186 121 83 53 BsaWI w'CCGG_w Cuts at: 5622 7080 7478 7752 7899 8310 5622 Size: 1458 398 274 147 411 5959 Fragments arranged by size: 5959 1458 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3885 3890 3915 3920 4790 Size: 30 165 30 2482 5 25 5 870 Cuts at: 4790 4820 4918 4948 1178 Size: 30 98 30 4877 Fragments arranged by size: 4877 2482 870 165 98 30 30 30 30 25 5 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2252 2419 2501 2936 3353 3440 3452 Size: 1063 3 167 82 435 417 87 12 Cuts at: 3452 3767 3818 3953 4226 4405 4682 4850 5768 Size: 315 51 135 273 179 277 168 918 Cuts at: 5768 5926 1186 Size: 158 3907 Fragments arranged by size: 3907 1063 918 435 417 315 277 273 179 168 167 158 135 87 82 51 12 3 BseYI C'CCAG_C Cuts at: 2155 2341 2707 2839 3031 3949 3960 4195 4300 Size: 186 366 132 192 918 11 235 105 Cuts at: 4300 5003 5084 5494 5590 7850 8346 2155 Size: 703 81 410 96 2260 496 2456 Fragments arranged by size: 2456 2260 918 703 496 410 366 235 192 186 132 105 96 81 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2114 5495 1163 Size: 573 378 3381 4315 Fragments arranged by size: 4315 3381 573 378 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6097 6858 7886 1540 Size: 275 186 4096 761 1028 2301 Fragments arranged by size: 4096 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2462 2912 3018 3081 3294 3360 3413 3903 4106 Size: 450 106 63 213 66 53 490 203 Cuts at: 4106 4557 4633 4659 4674 5529 6708 6793 7864 Size: 451 76 26 15 855 1179 85 1071 Cuts at: 7864 2462 Size: 3245 Fragments arranged by size: 3245 1179 1071 855 490 451 450 213 203 106 85 76 66 63 53 26 15 BsmI GAATG_Cn' Cuts at: 6158 6251 6158 Size: 93 8554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2442 2462 2663 2830 2912 3018 3053 Size: 399 652 20 201 167 82 106 35 Cuts at: 3053 3081 3294 3326 3360 3413 3903 4106 4385 Size: 28 213 32 34 53 490 203 279 Cuts at: 4385 4557 4633 4659 4674 4751 4897 5529 5589 Size: 172 76 26 15 77 146 632 60 Cuts at: 5589 5966 6708 6793 7864 1391 Size: 377 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 652 632 490 399 377 279 213 203 201 172 167 146 106 85 82 77 76 60 53 35 34 32 28 26 20 15 BspEI T'CCGG_A Cuts at: 8310 8310 Size: 8647 BspHI T'CATG_A Cuts at: 3018 4080 4269 6536 8266 3018 Size: 1062 189 2267 1730 3399 Fragments arranged by size: 3399 2267 1730 1062 189 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2202 2346 3225 3816 3846 5231 6002 844 Size: 1358 144 879 591 30 1385 771 3489 Fragments arranged by size: 3489 1385 1358 879 771 591 144 30 BsrBI CCG'CTC Cuts at: 1394 1823 2379 3297 3442 5182 6534 1394 Size: 429 556 918 145 1740 1352 3507 Fragments arranged by size: 3507 1740 1352 918 556 429 145 BsrDI GCAATG_nn' Cuts at: 65 1248 3280 7142 7316 65 Size: 1183 2032 3862 174 1396 Fragments arranged by size: 3862 2032 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 2787 3344 3681 4377 5199 7291 Size: 110 211 766 557 337 696 822 2092 Cuts at: 7291 7478 1700 Size: 187 2869 Fragments arranged by size: 2869 2092 822 766 696 557 337 211 187 110 BsrGI T'GTAC_A Cuts at: 96 2394 4275 96 Size: 2298 1881 4468 Fragments arranged by size: 4468 2298 1881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8647 BssSI C'ACGA_G Cuts at: 6707 7719 6707 Size: 1012 7635 BstAPI GCAn_nnn'nTGC Cuts at: 794 5732 794 Size: 4938 3709 BstBI TT'CG_AA Cuts at: 7451 7451 Size: 8647 BstEII G'GTnAC_C Cuts at: 1184 5401 1184 Size: 4217 4430 BstXI CCAn_nnnn'nTGG Cuts at: 2689 5452 5810 2689 Size: 2763 358 5526 Fragments arranged by size: 5526 2763 358 BstYI r'GATC_y Cuts at: 1972 3207 3522 4908 5154 5536 5977 6344 6729 Size: 1235 315 1386 246 382 441 367 385 Cuts at: 6729 6746 8198 8284 8296 8642 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1386 1235 441 385 382 367 346 315 246 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3239 3239 Size: 8647 BtgI C'CryG_G Cuts at: 513 1065 2004 2736 3072 3915 4088 4851 513 Size: 552 939 732 336 843 173 763 4309 Fragments arranged by size: 4309 939 843 763 732 552 336 173 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7908 Fragments arranged by size: 7908 683 56 BtsI GCAGTG_nn' Cuts at: 2661 2862 3382 3995 5463 5601 6163 6957 6977 Size: 201 520 613 1468 138 562 794 20 Cuts at: 6977 2661 Size: 4331 Fragments arranged by size: 4331 1468 794 613 562 520 201 138 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5209 5244 529 Size: 35 4645 35 3932 Fragments arranged by size: 4645 3932 35 35 DraI TTT'AAA Cuts at: 5951 5951 Size: 8647 DraIII CAC_nnn'GTG Cuts at: 2810 3375 2810 Size: 565 8082 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5237 7654 809 Size: 507 3921 2417 1802 Fragments arranged by size: 3921 2417 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3625 4611 4782 Size: 54 1425 50 275 18 1795 986 171 Cuts at: 4782 5812 6094 6983 8612 8 Size: 1030 282 889 1629 43 Fragments arranged by size: 1795 1629 1425 1030 986 889 282 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6094 1537 Size: 275 4282 4090 Fragments arranged by size: 4282 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2143 3110 3287 4680 6577 1353 Size: 790 967 177 1393 1897 3423 Fragments arranged by size: 3423 1897 1393 967 790 177 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4364 5066 7234 7608 7754 387 Size: 1064 138 2775 702 2168 374 146 1280 Fragments arranged by size: 2775 2168 1280 1064 702 374 146 138 EcoICRI GAG'CTC Cuts at: 2910 3358 3901 4672 2910 Size: 448 543 771 6885 Fragments arranged by size: 6885 771 543 448 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3373 3548 3730 3887 3917 4945 4994 Size: 393 1741 175 182 157 30 1028 49 Cuts at: 4994 5198 6710 7307 8094 8362 1239 Size: 204 1512 597 787 268 1524 Fragments arranged by size: 1741 1524 1512 1028 787 597 393 268 204 182 175 157 49 30 EcoNI CCTnn'n_nnAGG Cuts at: 3929 5063 3929 Size: 1134 7513 EcoO109I rG'GnC_Cy Cuts at: 2419 2686 2800 3085 3823 3976 4149 4777 5069 Size: 267 114 285 738 153 173 628 292 Cuts at: 5069 5758 2419 Size: 689 5308 Fragments arranged by size: 5308 738 689 628 292 285 267 173 153 114 EcoRI G'AATT_C Cuts at: 5132 5956 6334 5132 Size: 824 378 7445 Fragments arranged by size: 7445 824 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2285 4660 5884 2285 Size: 2375 1224 5048 Fragments arranged by size: 5048 2375 1224 FseI GG_CCGG'CC Cuts at: 3348 3348 Size: 8647 FspI TGC'GCA Cuts at: 2540 2990 7153 2540 Size: 450 4163 4034 Fragments arranged by size: 4163 4034 450 HaeII r_GCGC'y Cuts at: 1678 3817 3967 4874 5176 5632 7794 1678 Size: 2139 150 907 302 456 2162 2531 Fragments arranged by size: 2531 2162 2139 907 456 302 150 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2663 2695 3139 3171 3890 Size: 32 135 32 1091 32 444 32 719 Cuts at: 3890 3922 4450 4482 7288 7320 7362 7394 1373 Size: 32 528 32 2806 32 42 32 2626 Fragments arranged by size: 2806 2626 1091 719 528 444 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3909 4416 5991 6237 669 Size: 988 281 1971 507 1575 246 3079 Fragments arranged by size: 3079 1971 1575 988 507 281 246 HindIII A'AGCT_T Cuts at: 748 3567 748 Size: 2819 5828 HpaI GTT'AAC Cuts at: 6237 6237 Size: 8647 KasI G'GCGC_C Cuts at: 4870 4870 Size: 8647 KpnI G_GTAC'C Cuts at: 4948 5972 4948 Size: 1024 7623 MfeI C'AATT_G Cuts at: 6246 6246 Size: 8647 MluI A'CGCG_T Cuts at: 8523 8523 Size: 8647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2476 2877 4055 7761 7945 1305 Size: 1171 401 1178 3706 184 2007 Fragments arranged by size: 3706 2007 1178 1171 401 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4613 4784 5814 10 Size: 54 1425 343 2781 171 1030 2843 Fragments arranged by size: 2843 2781 1425 1030 343 171 54 MslI CAynn'nnrTG Cuts at: 518 1166 2390 3416 3497 3641 4179 5141 5276 Size: 648 1224 1026 81 144 538 962 135 Cuts at: 5276 5450 5471 5522 5888 6605 6964 7123 518 Size: 174 21 51 366 717 359 159 2042 Fragments arranged by size: 2042 1224 1026 962 717 648 538 366 359 174 159 144 135 81 51 21 MspA1I CmG'CkG Cuts at: 1606 2469 2585 3478 4067 4300 4885 5344 5375 Size: 863 116 893 589 233 585 459 31 Cuts at: 5375 5732 5942 6063 6740 7888 8133 1606 Size: 357 210 121 677 1148 245 2120 Fragments arranged by size: 2120 1148 893 863 677 589 585 459 357 245 233 210 121 116 31 NaeI GCC'GGC Cuts at: 2023 3346 5201 2023 Size: 1323 1855 5469 Fragments arranged by size: 5469 1855 1323 NarI GG'CG_CC Cuts at: 4871 4871 Size: 8647 NcoI C'CATG_G Cuts at: 513 1065 2004 3915 4851 513 Size: 552 939 1911 936 4309 Fragments arranged by size: 4309 1911 939 936 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8647 NgoMIV G'CCGG_C Cuts at: 2021 3344 5199 2021 Size: 1323 1855 5469 Fragments arranged by size: 5469 1855 1323 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8647 NotI GC'GGCC_GC Cuts at: 6094 6094 Size: 8647 NspI r_CATG'y Cuts at: 1132 1229 2029 2150 2391 3640 6005 7550 1132 Size: 97 800 121 241 1249 2365 1545 2229 Fragments arranged by size: 2365 2229 1545 1249 800 241 121 97 PasI CC'CwG_GG Cuts at: 1348 2434 4890 1348 Size: 1086 2456 5105 Fragments arranged by size: 5105 2456 1086 PciI A'CATG_T Cuts at: 7546 7546 Size: 8647 PflMI CCAn_nnn'nTGG Cuts at: 1108 3088 3372 3375 4300 5597 1108 Size: 1980 284 3 925 1297 4158 Fragments arranged by size: 4158 1980 1297 925 284 3 PfoI T'CCnGG_A Cuts at: 2289 3087 4254 2289 Size: 798 1167 6682 Fragments arranged by size: 6682 1167 798 PmeI GTTT'AAAC Cuts at: 5951 5951 Size: 8647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6716 6748 8229 8261 6716 Size: 32 1481 32 7102 Fragments arranged by size: 7102 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2419 2800 3085 4149 5069 5758 2419 Size: 381 285 1064 920 689 5308 Fragments arranged by size: 5308 1064 920 689 381 285 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8647 PsiI TTA'TAA Cuts at: 6217 6217 Size: 8647 PspOMI G'GGCC_C Cuts at: 4893 4893 Size: 8647 PstI C_TGCA'G Cuts at: 830 2799 3354 3612 3813 5040 5643 5999 830 Size: 1969 555 258 201 1227 603 356 3478 Fragments arranged by size: 3478 1969 1227 603 555 356 258 201 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8647 PvuII CAG'CTG Cuts at: 2469 4300 5344 5375 5732 5942 2469 Size: 1831 1044 31 357 210 5174 Fragments arranged by size: 5174 1831 1044 357 210 31 SacI G_AGCT'C Cuts at: 2912 3360 3903 4674 2912 Size: 448 543 771 6885 Fragments arranged by size: 6885 771 543 448 SalI G'TCGA_C Cuts at: 1936 5989 1936 Size: 4053 4594 SapI GCTCTTCn'nnn_ Cuts at: 3110 3110 Size: 8647 SbfI CC_TGCA'GG Cuts at: 2799 3354 5999 2799 Size: 555 2645 5447 Fragments arranged by size: 5447 2645 555 ScaI AGT'ACT Cuts at: 5929 6895 8607 5929 Size: 966 1712 5969 Fragments arranged by size: 5969 1712 966 SexAI A'CCwGG_T Cuts at: 2817 3081 3371 5352 2817 Size: 264 290 1981 6112 Fragments arranged by size: 6112 1981 290 264 SfcI C'TryA_G Cuts at: 826 1045 2047 2219 2795 3203 3350 3512 3608 Size: 219 1002 172 576 408 147 162 96 Cuts at: 3608 3809 4624 5036 5060 5639 5895 5995 7130 Size: 201 815 412 24 579 256 100 1135 Cuts at: 7130 7811 8002 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1002 815 681 579 576 412 408 256 219 201 191 172 162 147 100 96 24 SfoI GGC'GCC Cuts at: 4872 4872 Size: 8647 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8647 SgrAI Cr'CCGG_yG Cuts at: 1700 4377 1700 Size: 2677 5970 SmaI CCC'GGG Cuts at: 1872 5974 1872 Size: 4102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3137 4634 4985 5207 6751 7652 Size: 197 926 1194 1497 351 222 1544 901 Cuts at: 7652 7914 820 Size: 262 1553 Fragments arranged by size: 1553 1544 1497 1194 926 901 351 262 222 197 SnaBI TAC'GTA Cuts at: 493 6438 493 Size: 5945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8647 SphI G_CATG'C Cuts at: 1132 6005 1132 Size: 4873 3774 SspI AAT'ATT Cuts at: 5 52 6571 5 Size: 47 6519 2081 Fragments arranged by size: 6519 2081 47 StuI AGG'CCT Cuts at: 1517 3758 1517 Size: 2241 6406 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 3915 4851 6086 513 Size: 552 939 9 1902 936 1235 3074 Fragments arranged by size: 3074 1902 1235 939 936 552 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3070 3196 4304 5098 5227 6674 6691 Size: 1255 1490 126 1108 794 129 1447 17 Cuts at: 6691 6844 7029 7474 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1490 1447 1255 1108 794 445 185 153 129 126 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2394 3660 4275 Size: 274 80 33 51 1443 417 1266 615 Cuts at: 4275 5927 6893 8605 96 Size: 1652 966 1712 138 Fragments arranged by size: 1712 1652 1443 1266 966 615 417 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3083 3115 3171 3203 3885 3917 4918 4950 5073 Size: 32 56 32 682 32 1001 32 123 Cuts at: 5073 5105 8607 8639 3083 Size: 32 3502 32 3091 Fragments arranged by size: 3502 3091 1001 682 123 56 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8647 XbaI T'CTAG_A Cuts at: 5983 5983 Size: 8647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2287 3976 4909 2287 Size: 1689 933 6025 Fragments arranged by size: 6025 1689 933 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8647 XmaI C'CCGG_G Cuts at: 1870 5972 1870 Size: 4102 4545 XmnI GAAnn'nnTTC Cuts at: 4433 5630 6776 4433 Size: 1197 1146 6304 Fragments arranged by size: 6304 1197 1146 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6455 276 Size: 53 83 186 936 4921 2468 Fragments arranged by size: 4921 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI