(Circular) (Six-base) MAPSORT of: pFN21AB9501.seq Check: 8288 from: 1 to: 8065 pFN21AB9501 8065 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5875 278 Size: 53 83 186 936 4339 2468 Fragments arranged by size: 4339 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5408 1937 Size: 3471 4594 Acc65I G'GTAC_C Cuts at: 5386 5386 Size: 8065 AclI AA'CG_TT Cuts at: 1563 6192 6565 1563 Size: 4629 373 3063 Fragments arranged by size: 4629 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2196 2240 2282 2588 3045 3245 3302 3432 Size: 564 44 42 306 457 200 57 130 Cuts at: 3432 4427 6128 7512 7780 1632 Size: 995 1701 1384 268 1917 Fragments arranged by size: 1917 1701 1384 995 564 457 306 268 200 130 57 44 42 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7868 AflIII A'CryG_T Cuts at: 1203 4899 6964 7941 1203 Size: 3696 2065 977 1327 Fragments arranged by size: 3696 2065 1327 977 AgeI A'CCGG_T Cuts at: 6896 6896 Size: 8065 AhdI GACnn_n'nnGTC Cuts at: 5318 6794 5318 Size: 1476 6589 AleI CACnn'nnGTG Cuts at: 1166 4158 1166 Size: 2992 5073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8065 AlwNI CAG_nnn'CTG Cuts at: 5808 7380 5808 Size: 1572 6493 ApaLI G'TGCA_C Cuts at: 2402 6122 7278 2402 Size: 3720 1156 3189 Fragments arranged by size: 3720 3189 1156 ApoI r'AATT_y Cuts at: 1493 2694 2799 3934 4622 5187 5374 5600 5752 Size: 1201 105 1135 688 565 187 226 152 Cuts at: 5752 7861 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1201 1135 688 565 226 187 152 105 AseI AT'TA_AT Cuts at: 160 1032 6619 160 Size: 872 5587 1606 Fragments arranged by size: 5587 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8065 AvaI C'yCGr_G Cuts at: 1870 1943 5390 1870 Size: 73 3447 4545 Fragments arranged by size: 4545 3447 73 AvrII C'CTAG_G Cuts at: 3880 3880 Size: 8065 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3817 3850 3817 Size: 33 8032 BamHI G'GATC_C Cuts at: 3378 5395 5762 3378 Size: 2017 367 5681 Fragments arranged by size: 5681 2017 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3799 4140 4322 5008 5386 Size: 325 217 627 2012 341 182 686 378 Cuts at: 5386 6841 618 Size: 1455 1842 Fragments arranged by size: 2012 1842 1455 686 627 378 341 325 217 182 BanII G_rGCy'C Cuts at: 1391 2931 3969 4335 5384 1391 Size: 1540 1038 366 1049 4072 Fragments arranged by size: 4072 1540 1049 1038 366 BbeI G_GCGC'C Cuts at: 4144 4144 Size: 8065 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4995 5314 928 Size: 971 3096 319 3679 Fragments arranged by size: 3679 3096 971 319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4811 4845 6256 6290 1903 Size: 34 50 34 2790 34 1411 34 3678 Fragments arranged by size: 3678 2790 1411 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5957 7167 5957 Size: 1210 6855 BclI T'GATC_A Cuts at: 1548 3126 3247 1548 Size: 1578 121 6366 Fragments arranged by size: 6366 1578 121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4054 6676 136 Size: 107 122 71 631 605 2382 2622 1525 Fragments arranged by size: 2622 2382 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2179 2441 4234 4287 8060 2179 Size: 262 1793 53 3773 2184 Fragments arranged by size: 3773 2184 1793 262 53 BlpI GC'TnA_GC Cuts at: 5482 5482 Size: 8065 Bme1580I G_kGCm'C Cuts at: 1790 2406 6126 7282 1790 Size: 616 3720 1156 2573 Fragments arranged by size: 3720 2573 1156 616 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8065 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2424 2577 2802 Size: 416 53 83 399 24 1007 153 225 Cuts at: 2802 3853 4035 5796 6754 442 Size: 1051 182 1761 958 1753 Fragments arranged by size: 1761 1753 1051 1007 958 416 399 225 182 153 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8065 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3762 3777 3901 4036 5294 5348 6725 1239 Size: 2523 15 124 135 1258 54 1377 2579 Fragments arranged by size: 2579 2523 1377 1258 135 124 54 15 Bpu10I CC'TnA_GC Cuts at: 5094 5094 Size: 8065 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2103 2624 2633 3581 6190 7055 7353 2103 Size: 521 9 948 2609 865 298 2815 Fragments arranged by size: 2815 2609 948 865 521 298 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2507 4625 6728 882 Size: 617 1008 2118 2103 2219 Fragments arranged by size: 2219 2118 2103 1008 617 BsaAI yAC'GTr Cuts at: 493 1204 5856 493 Size: 711 4652 2702 Fragments arranged by size: 4652 2702 711 BsaBI GATnn'nnATC Cuts at: 2309 3569 4391 4518 8059 2309 Size: 1260 822 127 3541 2315 Fragments arranged by size: 3541 2315 1260 822 127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4141 5872 6254 6889 Size: 53 83 186 936 2608 1731 382 635 Cuts at: 6889 275 Size: 1451 Fragments arranged by size: 2608 1731 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6498 6896 7170 7317 7728 6498 Size: 398 274 147 411 6835 Fragments arranged by size: 6835 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2115 2145 2718 2748 4069 Size: 30 165 30 712 30 573 30 1321 Cuts at: 4069 4099 4425 4455 4698 4714 4728 4744 1178 Size: 30 326 30 243 16 14 16 4499 Fragments arranged by size: 4499 1321 712 573 326 243 165 30 30 30 30 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2040 2681 4594 1186 Size: 854 641 1913 4657 Fragments arranged by size: 4657 1913 854 641 BseYI C'CCAG_C Cuts at: 2088 5198 7268 7764 2088 Size: 3110 2070 496 2389 Fragments arranged by size: 3110 2389 2070 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4182 4524 4962 1163 Size: 573 2446 342 438 4266 Fragments arranged by size: 4266 2446 573 438 342 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5515 6276 7304 1540 Size: 275 186 3514 761 1028 2301 Fragments arranged by size: 3514 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2406 2931 6126 6211 7282 2406 Size: 525 3195 85 1071 3189 Fragments arranged by size: 3195 3189 1071 525 85 BsmI GAATG_Cn' Cuts at: 2083 5270 5576 5669 2083 Size: 3187 306 93 4479 Fragments arranged by size: 4479 3187 306 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2406 2931 3969 4335 5384 6126 6211 Size: 399 616 525 1038 366 1049 742 85 Cuts at: 6211 7282 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1049 1038 742 616 525 399 366 85 BspEI T'CCGG_A Cuts at: 7728 7728 Size: 8065 BspHI T'CATG_A Cuts at: 3837 5954 7684 3837 Size: 2117 1730 4218 Fragments arranged by size: 4218 2117 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2634 4157 5420 844 Size: 1790 1523 1263 3489 Fragments arranged by size: 3489 1790 1523 1263 BsrBI CCG'CTC Cuts at: 1394 1823 5952 1394 Size: 429 4129 3507 Fragments arranged by size: 4129 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5345 6560 6734 65 Size: 1183 4097 1215 174 1396 Fragments arranged by size: 4097 1396 1215 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2903 6709 6896 1700 Size: 110 1093 3806 187 2869 Fragments arranged by size: 3806 2869 1093 187 110 BsrGI T'GTAC_A Cuts at: 96 2292 96 Size: 2196 5869 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8065 BssSI C'ACGA_G Cuts at: 4358 6125 7137 4358 Size: 1767 1012 5286 Fragments arranged by size: 5286 1767 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2687 3443 4019 4322 5223 794 Size: 1893 756 576 303 901 3636 Fragments arranged by size: 3636 1893 901 756 576 303 BstBI TT'CG_AA Cuts at: 6869 6869 Size: 8065 BstEII G'GTnAC_C Cuts at: 1184 2356 2956 1184 Size: 1172 600 6293 Fragments arranged by size: 6293 1172 600 BstXI CCAn_nnnn'nTGG Cuts at: 2968 3315 2968 Size: 347 7718 BstYI r'GATC_y Cuts at: 1972 2179 2441 2758 3378 3564 4234 4287 5395 Size: 207 262 317 620 186 670 53 1108 Cuts at: 5395 5762 6147 6164 7616 7702 7714 8060 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1108 670 620 385 367 346 317 262 207 186 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7513 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7326 Fragments arranged by size: 7326 683 56 BtsI GCAGTG_nn' Cuts at: 3640 4950 5083 5581 6375 6395 3640 Size: 1310 133 498 794 20 5310 Fragments arranged by size: 5310 1310 794 498 133 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8065 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2440 2475 529 Size: 35 1876 35 6119 Fragments arranged by size: 6119 1876 35 35 DraI TTT'AAA Cuts at: 5369 5369 Size: 8065 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4124 7072 809 Size: 507 2808 2948 1802 Fragments arranged by size: 2948 2808 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 3637 5512 Size: 54 1425 50 275 18 402 1405 1875 Cuts at: 5512 6401 8030 8 Size: 889 1629 43 Fragments arranged by size: 1875 1629 1425 1405 889 402 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5512 1537 Size: 275 3700 4090 Fragments arranged by size: 4090 3700 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2669 2936 3057 4098 4787 5995 1353 Size: 1316 267 121 1041 689 1208 3423 Fragments arranged by size: 3423 1316 1208 1041 689 267 121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6652 7026 7172 387 Size: 1064 138 5063 374 146 1280 Fragments arranged by size: 5063 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2929 2929 Size: 8065 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2240 2282 2588 3045 3245 3302 Size: 393 564 44 42 306 457 200 57 Cuts at: 3302 3432 3762 3777 3901 4036 4427 5294 5348 Size: 130 330 15 124 135 391 867 54 Cuts at: 5348 6128 6725 7512 7780 1239 Size: 780 597 787 268 1524 Fragments arranged by size: 1524 867 787 780 597 564 457 393 391 330 306 268 200 135 130 124 57 54 44 42 15 EcoNI CCTnn'n_nnAGG Cuts at: 2121 2275 4913 2121 Size: 154 2638 5273 Fragments arranged by size: 5273 2638 154 EcoRI G'AATT_C Cuts at: 5374 5752 5374 Size: 378 7687 EcoRV GAT'ATC Cuts at: 2149 2149 Size: 8065 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2287 2620 2287 Size: 333 7732 FspI TGC'GCA Cuts at: 2660 6571 2660 Size: 3911 4154 HaeII r_GCGC'y Cuts at: 1678 4144 7212 1678 Size: 2466 3068 2531 Fragments arranged by size: 3068 2531 2466 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2269 2301 2340 2372 2426 Size: 32 135 32 697 32 39 32 54 Cuts at: 2426 2458 3244 3276 3337 3369 3854 3886 4425 Size: 32 786 32 61 32 485 32 539 Cuts at: 4425 4457 4622 4654 4698 4730 5074 5106 5304 Size: 32 165 32 44 32 344 32 198 Cuts at: 5304 5336 6706 6738 6780 6812 1373 Size: 32 1370 32 42 32 2626 Fragments arranged by size: 2626 1370 786 697 539 485 344 198 165 135 61 54 44 42 39 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2719 3211 5035 5409 5655 669 Size: 988 281 781 492 1824 374 246 3079 Fragments arranged by size: 3079 1824 988 781 492 374 281 246 HindIII A'AGCT_T Cuts at: 748 3551 748 Size: 2803 5262 HpaI GTT'AAC Cuts at: 5035 5655 5035 Size: 620 7445 KasI G'GCGC_C Cuts at: 4140 4140 Size: 8065 KpnI G_GTAC'C Cuts at: 5390 5390 Size: 8065 MfeI C'AATT_G Cuts at: 4641 5664 4641 Size: 1023 7042 MluI A'CGCG_T Cuts at: 7941 7941 Size: 8065 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2748 4411 7179 7363 1305 Size: 1443 1663 2768 184 2007 Fragments arranged by size: 2768 2007 1663 1443 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2234 10 Size: 54 1425 343 402 5841 Fragments arranged by size: 5841 1425 402 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4158 4358 6023 6382 6541 518 Size: 648 2992 200 1665 359 159 2042 Fragments arranged by size: 2992 2042 1665 648 359 200 159 MspA1I CmG'CkG Cuts at: 1606 2092 2260 2382 2968 3576 4319 5481 6158 Size: 486 168 122 586 608 743 1162 677 Cuts at: 6158 7306 7551 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1162 1148 743 677 608 586 486 245 168 122 NarI GG'CG_CC Cuts at: 4141 4141 Size: 8065 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7513 NdeI CA'TA_TG Cuts at: 387 387 Size: 8065 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8065 NotI GC'GGCC_GC Cuts at: 5512 5512 Size: 8065 NspI r_CATG'y Cuts at: 1132 1229 2638 2659 3755 4903 5423 6968 1132 Size: 97 1409 21 1096 1148 520 1545 2229 Fragments arranged by size: 2229 1545 1409 1148 1096 520 97 21 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8065 PciI A'CATG_T Cuts at: 4899 6964 4899 Size: 2065 6000 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8065 PfoI T'CCnGG_A Cuts at: 3501 5325 3501 Size: 1824 6241 PmeI GTTT'AAAC Cuts at: 5369 5369 Size: 8065 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6134 6166 7647 7679 6134 Size: 32 1481 32 6520 Fragments arranged by size: 6520 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2039 1613 Size: 426 7639 PsiI TTA'TAA Cuts at: 5635 5635 Size: 8065 PstI C_TGCA'G Cuts at: 830 3160 5078 5417 830 Size: 2330 1918 339 3478 Fragments arranged by size: 3478 2330 1918 339 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8065 PvuII CAG'CTG Cuts at: 2092 2260 2382 2968 4319 2092 Size: 168 122 586 1351 5838 Fragments arranged by size: 5838 1351 586 168 122 SacI G_AGCT'C Cuts at: 2931 2931 Size: 8065 SalI G'TCGA_C Cuts at: 1936 5407 1936 Size: 3471 4594 SapI GCTCTTCn'nnn_ Cuts at: 2669 2936 4787 2669 Size: 267 1851 5947 Fragments arranged by size: 5947 1851 267 SbfI CC_TGCA'GG Cuts at: 5417 5417 Size: 8065 ScaI AGT'ACT Cuts at: 4340 6313 8025 4340 Size: 1973 1712 4380 Fragments arranged by size: 4380 1973 1712 SfcI C'TryA_G Cuts at: 826 1045 3156 5074 5413 6548 7229 7420 826 Size: 219 2111 1918 339 1135 681 191 1471 Fragments arranged by size: 2111 1918 1471 1135 681 339 219 191 SfoI GGC'GCC Cuts at: 4142 4142 Size: 8065 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8065 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8065 SmaI CCC'GGG Cuts at: 1872 5392 1872 Size: 3520 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2118 2639 2648 3560 6169 7070 Size: 197 926 175 521 9 912 2609 901 Cuts at: 7070 7332 820 Size: 262 1553 Fragments arranged by size: 2609 1553 926 912 901 521 262 197 175 9 SnaBI TAC'GTA Cuts at: 493 5856 493 Size: 5363 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8065 SphI G_CATG'C Cuts at: 1132 2638 2659 5423 1132 Size: 1506 21 2764 3774 Fragments arranged by size: 3774 2764 1506 21 SspI AAT'ATT Cuts at: 5 52 3164 4874 5989 5 Size: 47 3112 1710 1115 2081 Fragments arranged by size: 3112 2081 1710 1115 47 StuI AGG'CCT Cuts at: 1517 4057 1517 Size: 2540 5525 StyI C'CwwG_G Cuts at: 513 1065 3880 5245 5504 513 Size: 552 2815 1365 259 3074 Fragments arranged by size: 3074 2815 1365 552 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5026 6092 6109 6262 6447 6892 325 Size: 1255 3446 1066 17 153 185 445 1498 Fragments arranged by size: 3446 1498 1255 1066 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2292 4338 6311 Size: 274 80 33 51 1443 315 2046 1973 Cuts at: 6311 8023 96 Size: 1712 138 Fragments arranged by size: 2046 1973 1712 1443 315 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2115 2147 2718 2750 4714 4746 8025 8057 2115 Size: 32 571 32 1964 32 3279 32 2123 Fragments arranged by size: 3279 2123 1964 571 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4109 1316 Size: 2793 5272 XbaI T'CTAG_A Cuts at: 5401 5401 Size: 8065 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4485 4852 4485 Size: 367 7698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8065 XmaI C'CCGG_G Cuts at: 1870 5390 1870 Size: 3520 4545 XmnI GAAnn'nnTTC Cuts at: 2181 6194 2181 Size: 4013 4052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5873 276 Size: 53 83 186 936 4339 2468 Fragments arranged by size: 4339 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI