Restriction map for
pFN21AB9497
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# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
ApoI AscI AseI AsnI AvrII
BbrPI BfrI BlnI BsaAI BseAI
BsiWI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EcoRI EcoRV EcoT22I
FseI HincII HindII HpaI KspI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SfiI SfuI SnaBI SpeI SphI
SspI SwaI TfiI Tsp509I XbaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 571 GACGTC GACGT^C
Acc65I 408 GGTACC G^GTACC
AccI 1722 GTCTAC GT^[AC][GT]AC
AhdI 1148 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1422 GTGCAC G^TGCAC
AocI 3519 CCTGAGG CC^T[ACGT]AGG
ApaLI 1422 GTGCAC G^TGCAC
Asp718I 408 GGTACC G^GTACC
AspEI 1148 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1481 TGCGCA TGC^GCA
BciVI 429 GGATAC GTATCC(6/5)
BglII 2127 AGATCT A^GATCT
BsaBI 1353 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3202 CGGCCG CG[GA][CT]^CG
BsmBI 2615 GAGACG CGTCTC(1/5)
BsrDI 1825 GCAATG GCAATG(2/0)
BssSI 22 CTCGTG CACGAG(-5/-1)
BstAPI 1460 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3519 CCTGAGG CC^T[ACGT]AGG
DraIII 151 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3202 CGGCCG C^GGCCG
Eam1105I 1148 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3202 CGGCCG C^GGCCG
Eco52I 3202 CGGCCG C^GGCCG
Eco81I 3519 CCTGAGG CC^T[ACGT]AGG
FspI 1481 TGCGCA TGC^GCA
HindIII 2587 AAGCTT A^AGCTT
KpnI 408 GGTACC GGTAC^C
MamI 1353 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2393 TTAA T^TAA
NspI 2606 GCATGT [GA]CATG^[CT]
PshAI 1773 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 3288 CCTGCAGG CCTGCA^GG
ScaI 2594 AGTACT AGT^ACT
SgrAI 1090 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 3288 CCTGCAGG CCTGCA^GG
Tru9I 2393 TTAA T^TAA
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 729 CGCG CG^CG
2218 CGCG
AgeI 40 ACCGGT A^CCGGT
654 ACCGGT
Asp700I 2487 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3453 GAAGCGCTTC
BamHI 176 GGATCC G^GATCC
2397 GGATCC
BbeI 2620 GGCGCC GGCGC^C
3189 GGCGCC
BcgI 461 CGAAGCGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
14 GCAGCCCTCTCG
BclI 644 TGATCA T^GATCA
2303 TGATCA
BsaWI 40 ACCGGT [AT]^CCGG[AT]
654 ACCGGT
BsmI 1031 GCATTC GAATGC(1/-1)
1588 GCATTC
Bsp1407I 809 TGTACA T^GTACA
2801 TGTACA
BspHI 2153 TCATGA T^CATGA
2915 TCATGA
BsrGI 809 TGTACA T^GTACA
2801 TGTACA
BstUI 729 CGCG CG^CG
2218 CGCG
FbaI 644 TGATCA T^GATCA
2303 TGATCA
HgaI 1853 GCGTC GACGC(5/10)
2780 GCGTC
KasI 2620 GGCGCC G^GCGCC
3189 GGCGCC
MvnI 729 CGCG CG^CG
2218 CGCG
NarI 2620 GGCGCC GG^CGCC
3189 GGCGCC
PaeR7I 2350 CTCGAG C^TCGAG
2706 CTCGAG
PflMI 672 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1776 CCAGGTCTTGG
PinAI 40 ACCGGT A^CCGGT
654 ACCGGT
RcaI 2153 TCATGA T^CATGA
2915 TCATGA
SfoI 2620 GGCGCC GGC^GCC
3189 GGCGCC
SmaI 1133 CCCGGG CCC^GGG
2267 CCCGGG
SmlI 2350 CTCGAG C^T[CT][GA]AG
2706 CTCGAG
SspBI 809 TGTACA T^GTACA
2801 TGTACA
StuI 716 AGGCCT AGG^CCT
1934 AGGCCT
Van91I 672 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1776 CCAGGTCTTGG
XhoI 2350 CTCGAG C^TCGAG
2706 CTCGAG
XmaCI 1133 CCCGGG C^CCGGG
2267 CCCGGG
XmaI 1133 CCCGGG C^CCGGG
2267 CCCGGG
XmnI 2487 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3453 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI Aor51HI ApaI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflFI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
Tth111I TthHB8I XcmI XhoII