(Circular) (Six-base) MAPSORT of: pFN21AB9497.seq Check: 1468 from: 1 to: 8230 pFN21AB9497 8230 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3409 3668 3409 Size: 259 7971 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2580 6040 278 Size: 53 83 186 936 1044 3460 2468 Fragments arranged by size: 3460 2468 1044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3728 5573 1937 Size: 1791 1845 4594 Fragments arranged by size: 4594 1845 1791 Acc65I G'GTAC_C Cuts at: 2413 5551 2413 Size: 3138 5092 AclI AA'CG_TT Cuts at: 1563 6357 6730 1563 Size: 4794 373 3063 Fragments arranged by size: 4794 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2710 3068 3602 4193 4580 4667 6293 7677 Size: 1078 358 534 591 387 87 1626 1384 Cuts at: 7677 7945 1632 Size: 268 1917 Fragments arranged by size: 1917 1626 1384 1078 591 534 387 358 268 87 AfeI AGC'GCT Cuts at: 2504 4941 5462 2504 Size: 2437 521 5272 Fragments arranged by size: 5272 2437 521 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8033 AflIII A'CryG_T Cuts at: 1203 7129 8106 1203 Size: 5926 977 1327 Fragments arranged by size: 5926 1327 977 AgeI A'CCGG_T Cuts at: 2045 2659 7061 2045 Size: 614 4402 3214 Fragments arranged by size: 4402 3214 614 AhdI GACnn_n'nnGTC Cuts at: 3158 6959 3158 Size: 3801 4429 AleI CACnn'nnGTG Cuts at: 1166 3692 4385 4907 5222 1166 Size: 2526 693 522 315 4174 Fragments arranged by size: 4174 2526 693 522 315 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3059 3994 4088 5021 1184 Size: 1875 935 94 933 4393 Fragments arranged by size: 4393 1875 935 933 94 AlwNI CAG_nnn'CTG Cuts at: 2683 4018 4037 4108 4204 4879 5973 7545 2683 Size: 1335 19 71 96 675 1094 1572 3368 Fragments arranged by size: 3368 1572 1335 1094 675 96 71 19 ApaI G_GGCC'C Cuts at: 3078 3639 4473 4645 3078 Size: 561 834 172 6663 Fragments arranged by size: 6663 834 561 172 ApaLI G'TGCA_C Cuts at: 3427 6287 7443 3427 Size: 2860 1156 4214 Fragments arranged by size: 4214 2860 1156 ApoI r'AATT_y Cuts at: 1493 5539 5765 5917 8026 1493 Size: 4046 226 152 2109 1697 Fragments arranged by size: 4046 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6784 160 Size: 872 5752 1606 Fragments arranged by size: 5752 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8230 AvaI C'yCGr_G Cuts at: 1870 1943 3138 3935 3986 4272 4355 4711 5555 Size: 73 1195 797 51 286 83 356 844 Cuts at: 5555 1870 Size: 4545 Fragments arranged by size: 4545 1195 844 797 356 286 83 73 51 BamHI G'GATC_C Cuts at: 2181 4402 5560 5927 2181 Size: 2221 1158 367 4484 Fragments arranged by size: 4484 2221 1158 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2413 2423 3305 3343 3394 Size: 325 217 627 626 10 882 38 51 Cuts at: 3394 3536 3574 3847 4452 4625 5194 5551 7006 Size: 142 38 273 605 173 569 357 1455 Cuts at: 7006 618 Size: 1842 Fragments arranged by size: 1842 1455 882 627 626 605 569 357 325 273 217 173 142 51 38 38 10 BanII G_rGCy'C Cuts at: 1391 3020 3078 3218 3373 3639 3819 3843 3978 Size: 1629 58 140 155 266 180 24 135 Cuts at: 3978 4473 4645 5258 5549 1391 Size: 495 172 613 291 4072 Fragments arranged by size: 4072 1629 613 495 291 266 180 172 155 140 135 58 24 BbeI G_GCGC'C Cuts at: 4629 5198 4629 Size: 569 7661 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3056 4571 4780 5131 928 Size: 971 1157 1515 209 351 4027 Fragments arranged by size: 4027 1515 1157 971 351 209 BbvCI CC'TCA_GC Cuts at: 2087 2600 3671 4463 5312 2087 Size: 513 1071 792 849 5005 Fragments arranged by size: 5005 1071 849 792 513 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2008 2042 2455 2489 6421 6455 1903 Size: 34 71 34 413 34 3932 34 3678 Fragments arranged by size: 3932 3678 413 71 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2428 6122 7332 2428 Size: 3694 1210 3326 Fragments arranged by size: 3694 3326 1210 BclI T'GATC_A Cuts at: 1548 2649 4308 1548 Size: 1101 1659 5470 Fragments arranged by size: 5470 1659 1101 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2990 3067 3634 Size: 107 122 71 631 605 1318 77 567 Cuts at: 3634 4550 4649 6841 136 Size: 916 99 2192 1525 Fragments arranged by size: 2192 1525 1318 916 631 605 567 122 107 99 77 71 BglII A'GATC_T Cuts at: 4132 8225 4132 Size: 4093 4137 BlpI GC'TnA_GC Cuts at: 2114 4979 4994 5647 2114 Size: 2865 15 653 4697 Fragments arranged by size: 4697 2865 653 15 Bme1580I G_kGCm'C Cuts at: 1790 2931 3078 3431 3539 3579 3639 3999 4086 Size: 1141 147 353 108 40 60 360 87 Cuts at: 4086 4473 4535 4645 4740 4989 5229 6291 7447 Size: 387 62 110 95 249 240 1062 1156 Cuts at: 7447 1790 Size: 2573 Fragments arranged by size: 2573 1156 1141 1062 387 360 353 249 240 147 110 108 95 87 62 60 40 BmgBI CAC'GTC Cuts at: 1318 2158 3160 3562 1318 Size: 840 1002 402 5986 Fragments arranged by size: 5986 1002 840 402 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4813 5961 6919 Size: 416 53 83 399 24 3396 1148 958 Cuts at: 6919 442 Size: 1753 Fragments arranged by size: 3396 1753 1148 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8230 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2375 2591 2899 3665 3764 4112 5411 6890 Size: 1136 216 308 766 99 348 1299 1479 Cuts at: 6890 1239 Size: 2579 Fragments arranged by size: 2579 1479 1299 1136 766 348 308 216 99 Bpu10I CC'TnA_GC Cuts at: 2087 2600 3671 4332 4463 4557 5252 5312 2087 Size: 513 1071 661 131 94 695 60 5005 Fragments arranged by size: 5005 1071 695 661 513 131 94 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6355 7220 7518 6355 Size: 865 298 7067 Fragments arranged by size: 7067 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2513 3793 4702 6893 882 Size: 617 1014 1280 909 2191 2219 Fragments arranged by size: 2219 2191 1280 1014 909 617 BsaAI yAC'GTr Cuts at: 493 1204 6021 493 Size: 711 4817 2702 Fragments arranged by size: 4817 2702 711 BsaBI GATnn'nnATC Cuts at: 3362 8224 3362 Size: 4862 3368 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2577 4626 5195 6037 Size: 53 83 186 936 1044 2049 569 842 Cuts at: 6037 6419 7054 275 Size: 382 635 1451 Fragments arranged by size: 2049 1451 1044 936 842 635 569 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2045 2659 6663 7061 7335 7482 7893 2045 Size: 614 4004 398 274 147 411 2382 Fragments arranged by size: 4004 2382 614 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8005 Fragments arranged by size: 8005 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2083 2474 3290 3851 1186 Size: 897 391 816 561 5565 Fragments arranged by size: 5565 897 816 561 391 BseYI C'CCAG_C Cuts at: 2177 2442 2523 2676 3070 3735 3819 4085 4101 Size: 265 81 153 394 665 84 266 16 Cuts at: 4101 5064 5350 7433 7929 2177 Size: 963 286 2083 496 2478 Fragments arranged by size: 2478 2083 963 665 496 394 286 266 265 153 84 81 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2345 3105 3175 5054 1163 Size: 573 609 760 70 1879 4339 Fragments arranged by size: 4339 1879 760 609 573 70 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5210 5680 6441 7469 1540 Size: 275 186 3209 470 761 1028 2301 Fragments arranged by size: 3209 2301 1028 761 470 275 186 BsiHKAI G_wGCw'C Cuts at: 3020 3218 3431 3652 4920 4995 5258 6291 6376 Size: 198 213 221 1268 75 263 1033 85 Cuts at: 6376 7447 3020 Size: 1071 3803 Fragments arranged by size: 3803 1268 1071 1033 263 221 213 198 85 75 BsmI GAATG_Cn' Cuts at: 3036 3593 5741 5834 3036 Size: 557 2148 93 5432 Fragments arranged by size: 5432 2148 557 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4614 4614 Size: 8230 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2931 3020 3078 3218 3373 3431 3539 Size: 399 1141 89 58 140 155 58 108 Cuts at: 3539 3579 3639 3652 3819 3843 3978 3999 4086 Size: 40 60 13 167 24 135 21 87 Cuts at: 4086 4473 4535 4645 4740 4920 4989 4995 5229 Size: 387 62 110 95 180 69 6 234 Cuts at: 5229 5258 5549 6291 6376 7447 1391 Size: 29 291 742 85 1071 2174 Fragments arranged by size: 2174 1141 1071 742 399 387 291 234 180 167 155 140 135 110 108 95 89 87 85 69 62 60 58 58 40 29 24 21 13 6 BspEI T'CCGG_A Cuts at: 7893 7893 Size: 8230 BspHI T'CATG_A Cuts at: 4158 4920 6119 7849 4158 Size: 762 1199 1730 4539 Fragments arranged by size: 4539 1730 1199 762 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3409 3668 4257 4878 5121 5585 844 Size: 2565 259 589 621 243 464 3489 Fragments arranged by size: 3489 2565 621 589 464 259 243 BsrBI CCG'CTC Cuts at: 1394 1823 2565 2586 4336 5212 6117 1394 Size: 429 742 21 1750 876 905 3507 Fragments arranged by size: 3507 1750 905 876 742 429 21 BsrDI GCAATG_nn' Cuts at: 65 1248 3837 6725 6899 65 Size: 1183 2589 2888 174 1396 Fragments arranged by size: 2888 2589 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2045 2659 2883 2993 3096 3675 3754 Size: 110 235 614 224 110 103 579 79 Cuts at: 3754 5027 5205 5330 5516 6874 7061 1700 Size: 1273 178 125 186 1358 187 2869 Fragments arranged by size: 2869 1358 1273 614 579 235 224 187 186 178 125 110 110 103 79 BsrGI T'GTAC_A Cuts at: 96 2814 4806 96 Size: 2718 1992 3520 Fragments arranged by size: 3520 2718 1992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8230 BssSI C'ACGA_G Cuts at: 2027 6290 7302 2027 Size: 4263 1012 2955 Fragments arranged by size: 4263 2955 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3471 794 Size: 2677 5553 BstBI TT'CG_AA Cuts at: 7034 7034 Size: 8230 BstEII G'GTnAC_C Cuts at: 1184 3172 3382 4865 5023 5359 1184 Size: 1988 210 1483 158 336 4055 Fragments arranged by size: 4055 1988 1483 336 210 158 BstXI CCAn_nnnn'nTGG Cuts at: 2960 4456 4522 4891 4909 2960 Size: 1496 66 369 18 6281 Fragments arranged by size: 6281 1496 369 66 18 BstYI r'GATC_y Cuts at: 1972 2080 2181 4132 4402 5560 5927 6312 6329 Size: 108 101 1951 270 1158 367 385 17 Cuts at: 6329 7781 7867 7879 8225 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1951 1452 1158 385 367 346 270 108 101 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5525 5525 Size: 8230 BtgI C'CryG_G Cuts at: 513 1065 2135 2557 2593 3387 3693 3705 4428 Size: 552 1070 422 36 794 306 12 723 Cuts at: 4428 4740 5069 5133 513 Size: 312 329 64 3610 Fragments arranged by size: 3610 1070 794 723 552 422 329 312 306 64 36 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2716 487 Size: 683 56 1490 6001 Fragments arranged by size: 6001 1490 683 56 BtsI GCAGTG_nn' Cuts at: 3056 3174 3482 3798 4070 4920 5746 6540 6560 Size: 118 308 316 272 850 826 794 20 Cuts at: 6560 3056 Size: 4726 Fragments arranged by size: 4726 850 826 794 316 308 272 118 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8230 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4618 4653 529 Size: 35 4054 35 4106 Fragments arranged by size: 4106 4054 35 35 DraI TTT'AAA Cuts at: 5534 5534 Size: 8230 DraIII CAC_nnn'GTG Cuts at: 2161 2161 Size: 8230 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7237 809 Size: 507 5921 1802 Fragments arranged by size: 5921 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2386 2560 2885 Size: 54 1425 50 275 18 556 174 325 Cuts at: 2885 2991 3654 3917 4059 4719 4881 4961 5097 Size: 106 663 263 142 660 162 80 136 Cuts at: 5097 5207 5487 5501 5677 6566 8195 8 Size: 110 280 14 176 889 1629 43 Fragments arranged by size: 1629 1425 889 663 660 556 325 280 275 263 176 174 162 142 136 110 106 80 54 50 43 18 14 EagI C'GGCC_G Cuts at: 1537 1812 5207 5677 1537 Size: 275 3395 470 4090 Fragments arranged by size: 4090 3395 470 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 2315 2495 2895 3116 3744 3886 6160 Size: 848 114 180 400 221 628 142 2274 Cuts at: 6160 1353 Size: 3423 Fragments arranged by size: 3423 2274 848 628 400 221 180 142 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5192 6817 7191 7337 387 Size: 1064 138 3603 1625 374 146 1280 Fragments arranged by size: 3603 1625 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3018 3216 5256 3018 Size: 198 2040 5992 Fragments arranged by size: 5992 2040 198 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2375 2591 2710 2899 3068 3602 3665 Size: 393 743 216 119 189 169 534 63 Cuts at: 3665 3764 4112 4193 4580 4667 5411 6293 6890 Size: 99 348 81 387 87 744 882 597 Cuts at: 6890 7677 7945 1239 Size: 787 268 1524 Fragments arranged by size: 1524 882 787 744 743 597 534 393 387 348 268 216 189 169 119 99 87 81 63 EcoNI CCTnn'n_nnAGG Cuts at: 2492 3545 4526 5294 2492 Size: 1053 981 768 5428 Fragments arranged by size: 5428 1053 981 768 EcoO109I rG'GnC_Cy Cuts at: 2055 3074 3075 3120 3205 3635 3636 3739 3777 Size: 1019 1 45 85 430 1 103 38 Cuts at: 3777 4212 4519 4641 4642 5379 2055 Size: 435 307 122 1 737 4906 Fragments arranged by size: 4906 1019 737 435 430 307 122 103 85 45 38 1 1 1 EcoRI G'AATT_C Cuts at: 5539 5917 5539 Size: 378 7852 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5479 5479 Size: 8230 FspI TGC'GCA Cuts at: 3488 6736 3488 Size: 3248 4982 HaeII r_GCGC'y Cuts at: 1678 2506 4629 4762 4943 4976 5198 5249 5312 Size: 828 2123 133 181 33 222 51 63 Cuts at: 5312 5464 7377 1678 Size: 152 1913 2531 Fragments arranged by size: 2531 2123 1913 828 222 181 152 133 63 51 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3144 3176 4430 4462 6871 Size: 32 135 32 1572 32 1254 32 2409 Cuts at: 6871 6903 6945 6977 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2409 1572 1254 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5574 5820 669 Size: 988 281 3636 246 3079 Fragments arranged by size: 3636 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4592 748 Size: 3844 4386 HpaI GTT'AAC Cuts at: 5820 5820 Size: 8230 KasI G'GCGC_C Cuts at: 4625 5194 4625 Size: 569 7661 KpnI G_GTAC'C Cuts at: 2417 5555 2417 Size: 3138 5092 MfeI C'AATT_G Cuts at: 5829 5829 Size: 8230 MluI A'CGCG_T Cuts at: 8106 8106 Size: 8230 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2183 7344 7528 1305 Size: 878 5161 184 2007 Fragments arranged by size: 5161 2007 878 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3656 3919 4061 4721 4883 Size: 54 1425 343 1824 263 142 660 162 Cuts at: 4883 10 Size: 3357 Fragments arranged by size: 3357 1824 1425 660 343 263 162 142 54 MslI CAynn'nnrTG Cuts at: 518 1166 2870 3041 3422 3692 3902 4385 4907 Size: 648 1704 171 381 270 210 483 522 Cuts at: 4907 5222 6188 6547 6706 518 Size: 315 966 359 159 2042 Fragments arranged by size: 2042 1704 966 648 522 483 381 359 315 270 210 171 159 MspA1I CmG'CkG Cuts at: 1606 2118 2680 3514 3823 4034 4259 4467 5305 Size: 512 562 834 309 211 225 208 838 Cuts at: 5305 5473 5509 5646 6323 7471 7716 1606 Size: 168 36 137 677 1148 245 2120 Fragments arranged by size: 2120 1148 838 834 677 562 512 309 245 225 211 208 168 137 36 NaeI GCC'GGC Cuts at: 2885 2995 3677 3756 5207 5518 2885 Size: 110 682 79 1451 311 5597 Fragments arranged by size: 5597 1451 682 311 110 79 NarI GG'CG_CC Cuts at: 4626 5195 4626 Size: 569 7661 NcoI C'CATG_G Cuts at: 513 1065 2135 2557 2593 3705 4740 5069 513 Size: 552 1070 422 36 1112 1035 329 3674 Fragments arranged by size: 3674 1112 1070 1035 552 422 329 36 NdeI CA'TA_TG Cuts at: 387 387 Size: 8230 NgoMIV G'CCGG_C Cuts at: 2883 2993 3675 3754 5205 5516 2883 Size: 110 682 79 1451 311 5597 Fragments arranged by size: 5597 1451 682 311 110 79 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8230 NotI GC'GGCC_GC Cuts at: 5677 5677 Size: 8230 NspI r_CATG'y Cuts at: 1132 1229 4615 5588 7133 1132 Size: 97 3386 973 1545 2229 Fragments arranged by size: 3386 2229 1545 973 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8230 PciI A'CATG_T Cuts at: 7129 7129 Size: 8230 PflMI CCAn_nnn'nTGG Cuts at: 1108 2683 3787 1108 Size: 1575 1104 5551 Fragments arranged by size: 5551 1575 1104 PfoI T'CCnGG_A Cuts at: 2550 2607 3681 3741 2550 Size: 57 1074 60 7039 Fragments arranged by size: 7039 1074 60 57 PmeI GTTT'AAAC Cuts at: 5534 5534 Size: 8230 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6299 6331 7812 7844 6299 Size: 32 1481 32 6685 Fragments arranged by size: 6685 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2055 3120 3739 3777 4212 4519 2055 Size: 1065 619 38 435 307 5766 Fragments arranged by size: 5766 1065 619 435 307 38 PshAI GACnn'nnGTC Cuts at: 1613 3782 1613 Size: 2169 6061 PsiI TTA'TAA Cuts at: 5800 5800 Size: 8230 PspOMI G'GGCC_C Cuts at: 3074 3635 4469 4641 3074 Size: 561 834 172 6663 Fragments arranged by size: 6663 834 561 172 PspXI vC'TCGA_Gb Cuts at: 4711 4711 Size: 8230 PstI C_TGCA'G Cuts at: 830 3513 3763 3828 3894 4023 4254 4875 5013 Size: 2683 250 65 66 129 231 621 138 Cuts at: 5013 5179 5298 5582 830 Size: 166 119 284 3478 Fragments arranged by size: 3478 2683 621 284 250 231 166 138 129 119 66 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8230 PvuII CAG'CTG Cuts at: 2680 3514 3823 4034 5473 2680 Size: 834 309 211 1439 5437 Fragments arranged by size: 5437 1439 834 309 211 SacI G_AGCT'C Cuts at: 3020 3218 5258 3020 Size: 198 2040 5992 Fragments arranged by size: 5992 2040 198 SalI G'TCGA_C Cuts at: 1936 5572 1936 Size: 3636 4594 SanDI GG'GwC_CC Cuts at: 2055 3120 3777 2055 Size: 1065 657 6508 Fragments arranged by size: 6508 1065 657 SapI GCTCTTCn'nnn_ Cuts at: 2201 2315 3744 2201 Size: 114 1429 6687 Fragments arranged by size: 6687 1429 114 SbfI CC_TGCA'GG Cuts at: 5298 5582 5298 Size: 284 7946 ScaI AGT'ACT Cuts at: 4601 6478 8190 4601 Size: 1877 1712 4641 Fragments arranged by size: 4641 1877 1712 SexAI A'CCwGG_T Cuts at: 2538 3378 4890 2538 Size: 840 1512 5878 Fragments arranged by size: 5878 1512 840 SfcI C'TryA_G Cuts at: 826 1045 3313 3509 3759 3824 3890 4019 4250 Size: 219 2268 196 250 65 66 129 231 Cuts at: 4250 4586 4871 5009 5175 5294 5578 6713 7394 Size: 336 285 138 166 119 284 1135 681 Cuts at: 7394 7585 826 Size: 191 1471 Fragments arranged by size: 2268 1471 1135 681 336 285 284 250 231 219 196 191 166 138 129 119 66 65 SfoI GGC'GCC Cuts at: 4627 5196 4627 Size: 569 7661 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8230 SgrAI Cr'CCGG_yG Cuts at: 1700 3096 1700 Size: 1396 6834 SmaI CCC'GGG Cuts at: 1872 3140 4274 5557 1872 Size: 1268 1134 1283 4545 Fragments arranged by size: 4545 1283 1268 1134 SmlI C'TyrA_G Cuts at: 820 1017 1943 4355 4711 6334 7235 7497 820 Size: 197 926 2412 356 1623 901 262 1553 Fragments arranged by size: 2412 1623 1553 926 901 356 262 197 SnaBI TAC'GTA Cuts at: 493 6021 493 Size: 5528 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8230 SphI G_CATG'C Cuts at: 1132 5588 1132 Size: 4456 3774 SrfI GCCC'GGGC Cuts at: 3140 3140 Size: 8230 SspI AAT'ATT Cuts at: 5 52 6154 5 Size: 47 6102 2081 Fragments arranged by size: 6102 2081 47 StuI AGG'CCT Cuts at: 1517 2723 3941 1517 Size: 1206 1218 5806 Fragments arranged by size: 5806 1218 1206 StyI C'CwwG_G Cuts at: 513 1065 2135 2557 2593 3705 4515 4740 5069 Size: 552 1070 422 36 1112 810 225 329 Cuts at: 5069 5187 5669 513 Size: 118 482 3074 Fragments arranged by size: 3074 1112 1070 810 552 482 422 329 225 118 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2896 3495 4340 4853 6257 6274 6427 Size: 1255 1316 599 845 513 1404 17 153 Cuts at: 6427 6612 7057 325 Size: 185 445 1498 Fragments arranged by size: 1498 1404 1316 1255 845 599 513 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2814 4599 4794 Size: 274 80 33 51 1443 837 1785 195 Cuts at: 4794 4806 6476 8188 96 Size: 12 1670 1712 138 Fragments arranged by size: 1785 1712 1670 1443 837 274 195 138 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8190 8222 8190 Size: 32 8198 Tth111I GACn'n_nGTC Cuts at: 1316 2576 2852 4892 1316 Size: 1260 276 2040 4654 Fragments arranged by size: 4654 2040 1260 276 XbaI T'CTAG_A Cuts at: 5566 5566 Size: 8230 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2381 2402 2424 2381 Size: 21 22 8187 Fragments arranged by size: 8187 22 21 XhoI C'TCGA_G Cuts at: 1943 4355 4711 1943 Size: 2412 356 5462 Fragments arranged by size: 5462 2412 356 XmaI C'CCGG_G Cuts at: 1870 3138 4272 5555 1870 Size: 1268 1134 1283 4545 Fragments arranged by size: 4545 1283 1268 1134 XmnI GAAnn'nnTTC Cuts at: 4496 5462 6359 4496 Size: 966 897 6367 Fragments arranged by size: 6367 966 897 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2578 6038 276 Size: 53 83 186 936 1044 3460 2468 Fragments arranged by size: 3460 2468 1044 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SfiI SwaI