Restriction map for
pFN21AB9491
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AvaII
AviII AvrII BanI BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BlnI BsaAI BsaHI BsiEI BsiWI
BsmBI Bsp1407I BspDI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MluI
NarI NcoI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 60 CGCG CG^CG
AccIII 2384 TCCGGA T^CCGGA
AclI 1109 AACGTT AA^CGTT
AflIII 2250 ACATGT A^C[GA][CT]GT
AlwNI 2104 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 3322 GGGCCC GGGCC^C
AseI 3121 ATTAAT AT^TAAT
AsnI 3121 ATTAAT AT^TAAT
AspI 456 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 9 CCCGGG C^[CT]CG[GA]G
BalI 1656 TGGCCA TGG^CCA
BamHI 3243 GGATCC G^GATCC
BcgI 134 GCAAGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 3133 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3133 GCTAAGC GC^T[ACGT]AGC
BsaBI 1576 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2384 TCCGGA [AT]^CCGG[AT]
BseAI 2384 TCCGGA T^CCGGA
BsgI 425 GTGCAG GTGCAG(16/14)
BsoBI 9 CCCGGG C^[CT]CG[GA]G
BspEI 2384 TCCGGA T^CCGGA
BspLU11I 2250 ACATGT A^CATGT
BspMI 1388 ACCTGC ACCTGC(4/8)
BsrFI 69 GCCGGC [GA]^CCGG[CT]
BstAPI 2145 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 60 CGCG CG^CG
BstXI 2702 CCAAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3133 GCTAAGC GC^T[ACGT]AGC
Cfr10I 69 GCCGGC [GA]^CCGG[CT]
DraII 3321 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoNI 1566 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3321 AGGGCCC [GA]G^G[ACGT]CC[CT]
FauI 2853 GCGGG CCCGC(4/6)
HaeII 3386 AGCGCC [GA]GCGC^[CT]
MamI 1576 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1656 TGGCCA TGG^CCA
MroI 2384 TCCGGA T^CCGGA
MscI 1656 TGGCCA TGG^CCA
MvnI 60 CGCG CG^CG
NaeI 69 GCCGGC GCC^GGC
NgoMIV 69 GCCGGC G^CCGGC
NheI 1092 GCTAGC G^CTAGC
PflFI 456 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3121 ATTAAT AT^TAAT
Psp1406I 1109 AACGTT AA^CGTT
SacI 2300 GAGCTC GAGCT^C
SapI 1297 GAAGAGC GCTCTTC(1/4)
ScaI 1617 AGTACT AGT^ACT
SmaI 9 CCCGGG CCC^GGG
Tth111I 456 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 74 CCACCGCACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 9 CCCGGG C^CCGGG
XmaI 9 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Asp700I 232 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1741 GAAAGGCTTC
BanII 2300 GAGCTC G[GA]GC[CT]^C
3322 GGGCCC
BsaI 1786 GAGACC GGTCTC(1/5)
3008 GAGACC
BsmFI 876 GGGAC GGGAC(10/14)
684 GTCCC
BsmI 1160 GAATGC GAATGC(1/-1)
2328 GCATTC
BsrDI 1999 GCAATG GCAATG(2/0)
3221 GCAATG
DraI 171 TTTAAA TTT^AAA
2784 TTTAAA
MaeII 780 ACGT A^CGT
1110 ACGT
NdeI 1471 CATATG CA^TATG
2595 CATATG
NlaIV 3243 GGATCC GG[ACGT]^[ACGT]CC
3322 GGGCCC
PflMI 1284 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1665 CCAAGATATGG
PstI 1703 CTGCAG CTGCA^G
2333 CTGCAG
SspI 270 AATATT AAT^ATT
1364 AATATT
Van91I 1284 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1665 CCAAGATATGG
XbaI 463 TCTAGA T^CTAGA
1317 TCTAGA
XmnI 232 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1741 GAAAGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NdeII
NlaIII NspI PleI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII