(Circular) (Six-base) MAPSORT of: pFN21AB9491.seq Check: 6238 from: 1 to: 8125 pFN21AB9491 8125 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5935 278 Size: 53 83 186 936 4399 2468 Fragments arranged by size: 4399 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5468 1937 Size: 3531 4594 Acc65I G'GTAC_C Cuts at: 5446 5446 Size: 8125 AclI AA'CG_TT Cuts at: 1563 3115 6252 6625 1563 Size: 1552 3137 373 3063 Fragments arranged by size: 3137 3063 1552 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2966 3117 3716 3737 4397 6188 7572 7840 Size: 1334 151 599 21 660 1791 1384 268 Cuts at: 7840 1632 Size: 1917 Fragments arranged by size: 1917 1791 1384 1334 660 599 268 151 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7928 AflIII A'CryG_T Cuts at: 1203 4255 7024 8001 1203 Size: 3052 2769 977 1327 Fragments arranged by size: 3052 2769 1327 977 AgeI A'CCGG_T Cuts at: 6956 6956 Size: 8125 AhdI GACnn_n'nnGTC Cuts at: 6854 6854 Size: 8125 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8125 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2220 2445 1184 Size: 1036 225 6864 Fragments arranged by size: 6864 1036 225 AlwNI CAG_nnn'CTG Cuts at: 4114 5868 7440 4114 Size: 1754 1572 4799 Fragments arranged by size: 4799 1754 1572 ApaI G_GGCC'C Cuts at: 5331 5331 Size: 8125 ApaLI G'TGCA_C Cuts at: 6182 7338 6182 Size: 1156 6969 ApoI r'AATT_y Cuts at: 1493 2060 2241 2250 2387 2495 2873 4718 4734 Size: 567 181 9 137 108 378 1845 16 Cuts at: 4734 5434 5660 5812 7921 1493 Size: 700 226 152 2109 1697 Fragments arranged by size: 2109 1845 1697 700 567 378 226 181 152 137 108 16 9 AseI AT'TA_AT Cuts at: 160 1032 5127 6679 160 Size: 872 4095 1552 1606 Fragments arranged by size: 4095 1606 1552 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8125 AvaI C'yCGr_G Cuts at: 1870 1943 2014 5450 1870 Size: 73 71 3436 4545 Fragments arranged by size: 4545 3436 73 71 BamHI G'GATC_C Cuts at: 5248 5455 5822 5248 Size: 207 367 7551 Fragments arranged by size: 7551 367 207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5446 6901 618 Size: 325 217 627 3659 1455 1842 Fragments arranged by size: 3659 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4309 5331 5444 1391 Size: 2918 1022 113 4072 Fragments arranged by size: 4072 2918 1022 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2574 2951 3636 3722 4588 928 Size: 971 675 377 685 86 866 4465 Fragments arranged by size: 4465 971 866 685 675 377 86 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2128 2162 6316 6350 1903 Size: 34 191 34 4154 34 3678 Fragments arranged by size: 4154 3678 191 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6017 7227 6017 Size: 1210 6915 BclI T'GATC_A Cuts at: 1548 1548 Size: 8125 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6736 136 Size: 107 122 71 631 605 5064 1525 Fragments arranged by size: 5064 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3136 4078 4615 8120 3136 Size: 942 537 3505 3141 Fragments arranged by size: 3505 3141 942 537 BlpI GC'TnA_GC Cuts at: 5139 5542 5139 Size: 403 7722 Bme1580I G_kGCm'C Cuts at: 1790 2100 4702 5331 6186 7342 1790 Size: 310 2602 629 855 1156 2573 Fragments arranged by size: 2602 2573 1156 855 629 310 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8125 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4126 4159 5856 Size: 416 53 83 399 24 2709 33 1697 Cuts at: 5856 6814 442 Size: 958 1753 Fragments arranged by size: 2709 1753 1697 958 416 399 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 3101 1056 Size: 2045 6080 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3264 5367 3264 Size: 2103 6022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3350 4136 5250 5352 5364 6785 1239 Size: 2111 786 1114 102 12 1421 2579 Fragments arranged by size: 2579 2111 1421 1114 786 102 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2726 4874 5089 6250 7115 7413 2726 Size: 2148 215 1161 865 298 3438 Fragments arranged by size: 3438 2148 1161 865 298 215 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3785 5007 6788 882 Size: 617 2286 1222 1781 2219 Fragments arranged by size: 2286 2219 1781 1222 617 BsaAI yAC'GTr Cuts at: 493 1204 5916 493 Size: 711 4712 2702 Fragments arranged by size: 4712 2702 711 BsaBI GATnn'nnATC Cuts at: 3585 8119 3585 Size: 4534 3591 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5932 6314 6949 275 Size: 53 83 186 936 4399 382 635 1451 Fragments arranged by size: 4399 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4389 6558 6956 7230 7377 7788 4389 Size: 2169 398 274 147 411 4726 Fragments arranged by size: 4726 2169 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2672 2702 1178 Size: 30 165 30 1269 30 6601 Fragments arranged by size: 6601 1269 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 2723 3428 4229 1186 Size: 1132 405 705 801 5082 Fragments arranged by size: 5082 1132 801 705 405 BseYI C'CCAG_C Cuts at: 2093 2151 2439 5388 7328 7824 2093 Size: 58 288 2949 1940 496 2394 Fragments arranged by size: 2949 2394 1940 496 288 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2451 1163 Size: 573 715 6837 Fragments arranged by size: 6837 715 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5575 6336 7364 1540 Size: 275 186 3574 761 1028 2301 Fragments arranged by size: 3574 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4309 4599 5206 6186 6271 7342 4309 Size: 290 607 980 85 1071 5092 Fragments arranged by size: 5092 1071 980 607 290 85 BsmI GAATG_Cn' Cuts at: 3171 4333 5636 5729 3171 Size: 1162 1303 93 5567 Fragments arranged by size: 5567 1303 1162 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 4309 4599 4702 5206 5331 5444 Size: 399 310 2209 290 103 504 125 113 Cuts at: 5444 6186 6271 7342 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2209 2174 1071 742 504 399 310 290 125 113 103 85 BspEI T'CCGG_A Cuts at: 4389 7788 4389 Size: 3399 4726 BspHI T'CATG_A Cuts at: 2230 4684 4821 6014 7744 2230 Size: 2454 137 1193 1730 2611 Fragments arranged by size: 2611 2454 1730 1193 137 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3402 5480 844 Size: 2558 2078 3489 Fragments arranged by size: 3489 2558 2078 BsrBI CCG'CTC Cuts at: 1394 1823 6012 1394 Size: 429 4189 3507 Fragments arranged by size: 4189 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4011 5233 6620 6794 65 Size: 1183 2763 1222 1387 174 1396 Fragments arranged by size: 2763 1396 1387 1222 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2074 6769 6956 1700 Size: 110 264 4695 187 2869 Fragments arranged by size: 4695 2869 264 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8125 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8125 BssSI C'ACGA_G Cuts at: 6185 7197 6185 Size: 1012 7113 BstAPI GCAn_nnn'nTGC Cuts at: 794 4156 794 Size: 3362 4763 BstBI TT'CG_AA Cuts at: 6929 6929 Size: 8125 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8125 BstXI CCAn_nnnn'nTGG Cuts at: 4714 4714 Size: 8125 BstYI r'GATC_y Cuts at: 1972 3136 4078 4615 5248 5383 5455 5822 6207 Size: 1164 942 537 633 135 72 367 385 Cuts at: 6207 6224 7676 7762 7774 8120 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1164 942 633 537 385 367 346 135 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6634 Fragments arranged by size: 6634 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7386 Fragments arranged by size: 7386 683 56 BtsI GCAGTG_nn' Cuts at: 5641 6435 6455 5641 Size: 794 20 7311 Fragments arranged by size: 7311 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8125 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8090 DraI TTT'AAA Cuts at: 2178 4791 5429 2178 Size: 2613 638 4874 Fragments arranged by size: 4874 2613 638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7132 809 Size: 507 5816 1802 Fragments arranged by size: 5816 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2076 3661 Size: 54 1425 50 275 18 225 21 1585 Cuts at: 3661 5572 6461 8090 8 Size: 1911 889 1629 43 Fragments arranged by size: 1911 1629 1585 1425 889 275 225 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 5572 1537 Size: 275 3760 4090 Fragments arranged by size: 4090 3760 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2475 3252 3297 3405 3570 3873 3960 3981 Size: 1122 777 45 108 165 303 87 21 Cuts at: 3981 4275 4425 6055 1353 Size: 294 150 1630 3423 Fragments arranged by size: 3423 1630 1122 777 303 294 165 150 108 87 45 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6712 7086 7232 387 Size: 1064 138 5123 374 146 1280 Fragments arranged by size: 5123 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4307 4307 Size: 8125 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2966 3117 3350 3716 3737 4136 4397 Size: 393 1334 151 233 366 21 399 261 Cuts at: 4397 5250 5352 5364 6188 6785 7572 7840 1239 Size: 853 102 12 824 597 787 268 1524 Fragments arranged by size: 1524 1334 853 824 787 597 399 393 366 268 261 233 151 102 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 3575 3575 Size: 8125 EcoO109I rG'GnC_Cy Cuts at: 5327 5327 Size: 8125 EcoRI G'AATT_C Cuts at: 5434 5812 5434 Size: 378 7747 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2879 5018 2879 Size: 2139 5986 FspI TGC'GCA Cuts at: 6631 6631 Size: 8125 HaeII r_GCGC'y Cuts at: 1678 5395 7272 1678 Size: 3717 1877 2531 Fragments arranged by size: 3717 2531 1877 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2672 2704 3200 Size: 32 135 32 614 32 454 32 496 Cuts at: 3200 3230 3232 3262 3919 3951 3983 4015 4334 Size: 30 2 30 657 32 32 32 319 Cuts at: 4334 4366 5004 5036 6766 6798 6840 6872 1373 Size: 32 638 32 1730 32 42 32 2626 Fragments arranged by size: 2626 1730 657 638 614 496 454 319 135 42 32 32 32 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 5469 5715 669 Size: 988 281 3531 246 3079 Fragments arranged by size: 3531 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2394 2978 3129 5361 748 Size: 1646 584 151 2232 3512 Fragments arranged by size: 3512 2232 1646 584 151 HpaI GTT'AAC Cuts at: 5715 5715 Size: 8125 KpnI G_GTAC'C Cuts at: 5450 5450 Size: 8125 MfeI C'AATT_G Cuts at: 3483 3858 4500 5724 3483 Size: 375 642 1224 5884 Fragments arranged by size: 5884 1224 642 375 MluI A'CGCG_T Cuts at: 8001 8001 Size: 8125 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2473 4794 7239 7423 1305 Size: 1168 2321 2445 184 2007 Fragments arranged by size: 2445 2321 2007 1168 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3663 10 Size: 54 1425 343 1831 4472 Fragments arranged by size: 4472 1831 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 3501 4488 4683 6083 6442 6601 Size: 648 1197 1138 987 195 1400 359 159 Cuts at: 6601 518 Size: 2042 Fragments arranged by size: 2042 1400 1197 1138 987 648 359 195 159 MspA1I CmG'CkG Cuts at: 1606 2089 3104 4186 4496 4541 5059 5541 6218 Size: 483 1015 1082 310 45 518 482 677 Cuts at: 6218 7366 7611 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1082 1015 677 518 483 482 310 245 45 NaeI GCC'GGC Cuts at: 2076 2076 Size: 8125 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6634 Fragments arranged by size: 6634 939 552 NdeI CA'TA_TG Cuts at: 387 3477 4601 387 Size: 3090 1124 3911 Fragments arranged by size: 3911 3090 1124 NgoMIV G'CCGG_C Cuts at: 2074 2074 Size: 8125 NheI G'CTAG_C Cuts at: 1052 3097 1052 Size: 2045 6080 NotI GC'GGCC_GC Cuts at: 5572 5572 Size: 8125 NspI r_CATG'y Cuts at: 1132 1229 2422 2447 4259 4493 5483 7028 1132 Size: 97 1193 25 1812 234 990 1545 2229 Fragments arranged by size: 2229 1812 1545 1193 990 234 97 25 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8125 PciI A'CATG_T Cuts at: 4255 7024 4255 Size: 2769 5356 PflMI CCAn_nnn'nTGG Cuts at: 1108 3295 3676 1108 Size: 2187 381 5557 Fragments arranged by size: 5557 2187 381 PmeI GTTT'AAAC Cuts at: 5429 5429 Size: 8125 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6194 6226 7707 7739 6194 Size: 32 1481 32 6580 Fragments arranged by size: 6580 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8125 PsiI TTA'TAA Cuts at: 2400 5695 2400 Size: 3295 4830 PspOMI G'GGCC_C Cuts at: 5327 5327 Size: 8125 PstI C_TGCA'G Cuts at: 830 3712 4342 5477 830 Size: 2882 630 1135 3478 Fragments arranged by size: 3478 2882 1135 630 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8125 PvuII CAG'CTG Cuts at: 2089 3104 4496 4541 5059 2089 Size: 1015 1392 45 518 5155 Fragments arranged by size: 5155 1392 1015 518 45 SacI G_AGCT'C Cuts at: 4309 4309 Size: 8125 SalI G'TCGA_C Cuts at: 1936 5467 1936 Size: 3531 4594 SapI GCTCTTCn'nnn_ Cuts at: 3297 3297 Size: 8125 SbfI CC_TGCA'GG Cuts at: 5477 5477 Size: 8125 ScaI AGT'ACT Cuts at: 3624 6373 8085 3624 Size: 2749 1712 3664 Fragments arranged by size: 3664 2749 1712 SfcI C'TryA_G Cuts at: 826 1045 2110 2834 2907 3708 4338 5473 6608 Size: 219 1065 724 73 801 630 1135 1135 Cuts at: 6608 7289 7480 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1135 1065 801 724 681 630 219 191 73 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8125 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8125 SmaI CCC'GGG Cuts at: 1872 2016 5452 1872 Size: 144 3436 4545 Fragments arranged by size: 4545 3436 144 SmlI C'TyrA_G Cuts at: 820 1017 1943 2705 4889 5104 6229 7130 7392 Size: 197 926 762 2184 215 1125 901 262 Cuts at: 7392 820 Size: 1553 Fragments arranged by size: 2184 1553 1125 926 901 762 262 215 197 SnaBI TAC'GTA Cuts at: 493 5916 493 Size: 5423 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8125 SphI G_CATG'C Cuts at: 1132 5483 1132 Size: 4351 3774 SspI AAT'ATT Cuts at: 5 52 2277 3371 6049 5 Size: 47 2225 1094 2678 2081 Fragments arranged by size: 2678 2225 2081 1094 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8125 StyI C'CwwG_G Cuts at: 513 1065 2004 2160 3045 3795 5564 513 Size: 552 939 156 885 750 1769 3074 Fragments arranged by size: 3074 1769 939 885 750 552 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6152 6169 6322 6507 6952 325 Size: 1255 4572 17 153 185 445 1498 Fragments arranged by size: 4572 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2533 3622 6371 Size: 274 80 33 51 1443 556 1089 2749 Cuts at: 6371 8083 96 Size: 1712 138 Fragments arranged by size: 2749 1712 1443 1089 556 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8085 8117 8085 Size: 32 8093 Tth111I GACn'n_nGTC Cuts at: 1316 2464 1316 Size: 1148 6977 XbaI T'CTAG_A Cuts at: 2468 3322 5461 2468 Size: 854 2139 5132 Fragments arranged by size: 5132 2139 854 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2086 2086 Size: 8125 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8125 XmaI C'CCGG_G Cuts at: 1870 2014 5450 1870 Size: 144 3436 4545 Fragments arranged by size: 4545 3436 144 XmnI GAAnn'nnTTC Cuts at: 2241 3750 6254 2241 Size: 1509 2504 4112 Fragments arranged by size: 4112 2504 1509 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5933 276 Size: 53 83 186 936 4399 2468 Fragments arranged by size: 4399 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI