Restriction map for
pFN21AB9486
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AclI AgeI AhdI AscI
AseI AsnI AspEI AviII AvrII
BbrPI BciVI BglII BlnI BlpI
Bpu1102I BsaBI BsiWI BsrBI BssHII
Bst1107I BstZ17I CelII CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoRV
FspI HincII HindII HindIII HpaI
MamI MfeI MluI MunI NcoI
NdeI NheI NotI NruI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I RsrII
SalI SapI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SphI
Sse8387I SwaI Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 638 GGTACC G^GTACC
AccIII 1518 TCCGGA T^CCGGA
AflII 334 CTTAAG C^TTAAG
Alw44I 3402 GTGCAC G^TGCAC
AocI 1465 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2465 AGCGCT AGC^GCT
ApaLI 3402 GTGCAC G^TGCAC
Asp718I 638 GGTACC G^GTACC
AspI 3432 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 83 TGGCCA TGG^CCA
BamHI 3552 GGATCC G^GATCC
BbsI 1422 GTCTTC GAAGAC(2/6)
BfrI 334 CTTAAG C^TTAAG
BglI 28 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1422 GTCTTC GAAGAC(2/6)
BseAI 1518 TCCGGA T^CCGGA
BspDI 2039 ATCGAT AT^CGAT
BspEI 1518 TCCGGA T^CCGGA
BspLU11I 1810 ACATGT A^CATGT
BssSI 892 CTCGTG CACGAG(-5/-1)
BstAPI 3641 GCAAGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2372 TTCGAA TT^CGAA
BstEII 586 GGTCACC G^GT[ACGT]ACC
BstPI 586 GGTCACC G^GT[ACGT]ACC
Bsu36I 1465 CCTCAGG CC^T[ACGT]AGG
ClaI 2039 ATCGAT AT^CGAT
DraI 985 TTTAAA TTT^AAA
DraIII 1554 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2465 AGCGCT AGC^GCT
Eco81I 1465 CCTCAGG CC^T[ACGT]AGG
EcoO65I 586 GGTCACC G^GT[ACGT]ACC
EcoRI 523 GAATTC G^AATTC
EcoT22I 701 ATGCAT ATGCA^T
FseI 3460 GGCCGGCC GGCCGG^CC
KpnI 638 GGTACC GGTAC^C
KspI 128 CCGCGG CCGC^GG
MluNI 83 TGGCCA TGG^CCA
MroI 1518 TCCGGA T^CCGGA
MscI 83 TGGCCA TGG^CCA
NsiI 701 ATGCAT ATGCA^T
NspV 2372 TTCGAA TT^CGAA
PflFI 3432 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 262 CGATCG CGAT^CG
SacII 128 CCGCGG CCGC^GG
SfuI 2372 TTCGAA TT^CGAA
SpeI 2163 ACTAGT A^CTAGT
Tth111I 3432 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
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AccI 205 GTAGAC GT^[AC][GT]AC
477 GTCTAC
AcyI 157 GGCGCC G[GA]^CG[CT]C
2317 GGCGCC
AflIII 481 ACGTGT A^C[GA][CT]GT
1810 ACATGT
Asp700I 2883 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3243 GAAGGGCTTC
BbeI 157 GGCGCC GGCGC^C
2317 GGCGCC
BcgI 3186 CGAGTGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3334 CGACGGGCTTGC
BsaAI 480 TACGTG [CT]AC^GT[GA]
598 CACGTA
BsaHI 157 GGCGCC G[GA]^CG[CT]C
2317 GGCGCC
BsiEI 122 CGGTCG CG[GA][CT]^CG
262 CGATCG
BsmBI 2658 CGTCTC CGTCTC(1/5)
2515 GAGACG
BsmI 273 GAATGC GAATGC(1/-1)
2913 GCATTC
Bsp1407I 605 TGTACA T^GTACA
3359 TGTACA
BsrFI 3155 GCCGGC [GA]^CCGG[CT]
3461 GCCGGC
BsrGI 605 TGTACA T^GTACA
3359 TGTACA
BstXI 372 CCAGGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2809 CCAGGTGAATGG
Cfr10I 3155 GCCGGC [GA]^CCGG[CT]
3461 GCCGGC
EcoNI 806 CCTATCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3048 CCTCTGCAAGG
Hin1I 157 GGCGCC G[GA]^CG[CT]C
2317 GGCGCC
KasI 157 GGCGCC G^GCGCC
2317 GGCGCC
NaeI 3155 GCCGGC GCC^GGC
3461 GCCGGC
NarI 157 GGCGCC GG^CGCC
2317 GGCGCC
NgoMIV 3155 GCCGGC G^CCGGC
3461 GCCGGC
NspI 1810 ACATGT [GA]CATG^[CT]
2824 ACATGC
PshAI 2517 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2993 GACCCTGGTC
SacI 831 GAGCTC GAGCT^C
1744 GAGCTC
SfoI 157 GGCGCC GGC^GCC
2317 GGCGCC
SspBI 605 TGTACA T^GTACA
3359 TGTACA
StuI 2256 AGGCCT AGG^CCT
2834 AGGCCT
TfiI 1332 GATTC G^A[AT]TC
1350 GAATC
XcmI 633 CCAGCGGTACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2554 CCAGGGATAGCTTGG
XmnI 2883 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3243 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BclI BcnI BfaI BmyI
BpmI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII