Restriction map for
pFN21AB9486
(Linear) (Six-base) MAPSORT of: KIBB9486.seq Check: 2685 from: 1 to: 3663
KIBB9486.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 206 478 3663
Size: 206 272 3185
Fragments arranged by size:
3185 272 206
Acc65I G'GTAC_C
Cuts at: 0 638 3663
Size: 638 3025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1388 3663
Size: 380 1008 2275
Fragments arranged by size:
2275 1008 380
AfeI AGC'GCT
Cuts at: 0 2467 3663
Size: 2467 1196
AflII C'TTAA_G
Cuts at: 0 334 3663
Size: 334 3329
AflIII A'CryG_T
Cuts at: 0 481 1810 3663
Size: 481 1329 1853
Fragments arranged by size:
1853 1329 481
AleI CACnn'nnGTG
Cuts at: 0 1632 1655 3663
Size: 1632 23 2008
Fragments arranged by size:
2008 1632 23
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3174 3206 3663
Size: 3174 32 457
Fragments arranged by size:
3174 457 32
AlwNI CAG_nnn'CTG
Cuts at: 0 321 552 1092 2343 2761 3538 3663
Size: 321 231 540 1251 418 777 125
Fragments arranged by size:
1251 777 540 418 321 231 125
ApaI G_GGCC'C
Cuts at: 0 52 1754 2507 3663
Size: 52 1702 753 1156
Fragments arranged by size:
1702 1156 753 52
ApaLI G'TGCA_C
Cuts at: 0 3402 3663
Size: 3402 261
ApoI r'AATT_y
Cuts at: 0 523 1450 2074 2531 3663
Size: 523 927 624 457 1132
Fragments arranged by size:
1132 927 624 523 457
AvaI C'yCGr_G
Cuts at: 0 412 1747 3079 3663
Size: 412 1335 1332 584
Fragments arranged by size:
1335 1332 584 412
BamHI G'GATC_C
Cuts at: 0 3552 3663
Size: 3552 111
BanI G'GyrC_C
Cuts at: 0 157 638 1634 2317 3663
Size: 157 481 996 683 1346
Fragments arranged by size:
1346 996 683 481 157
BanII G_rGCy'C
Cuts at: 0 52 835 1493 1748 1754 1956 2507 2768
Size: 52 783 658 255 6 202 551 261
Cuts at: 2768 3061 3663
Size: 293 602
Fragments arranged by size:
783 658 602 551 293 261 255 202
52 6
BbeI G_GCGC'C
Cuts at: 0 161 2321 3663
Size: 161 2160 1342
Fragments arranged by size:
2160 1342 161
BbsI GAAGACnn'nnnn_
Cuts at: 0 1415 3663
Size: 1415 2248
BbvCI CC'TCA_GC
Cuts at: 0 545 1456 3596 3663
Size: 545 911 2140 67
Fragments arranged by size:
2140 911 545 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3175 3209 3323 3357 3663
Size: 3175 34 114 34 306
Fragments arranged by size:
3175 306 114 34 34
BclI T'GATC_A
Cuts at: 0 1067 1149 2175 3663
Size: 1067 82 1026 1488
Fragments arranged by size:
1488 1067 1026 82
BfrBI ATG'CAT
Cuts at: 0 703 3663
Size: 703 2960
BglI GCCn_nnn'nGGC
Cuts at: 0 34 3663
Size: 34 3629
Bme1580I G_kGCm'C
Cuts at: 0 52 1319 1754 2507 3406 3663
Size: 52 1267 435 753 899 257
Fragments arranged by size:
1267 899 753 435 257 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 82 3663
Size: 82 3581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1076 2340 2430 2652 2864 3663
Size: 79 997 1264 90 222 212 799
Fragments arranged by size:
1264 997 799 222 212 90 79
Bpu10I CC'TnA_GC
Cuts at: 0 135 545 1456 3110 3596 3663
Size: 135 410 911 1654 486 67
Fragments arranged by size:
1654 911 486 410 135 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1429 1722 2702 3456 3663
Size: 1429 293 980 754 207
Fragments arranged by size:
1429 980 754 293 207
BsaI GGTCTCn'nnnn_
Cuts at: 0 1654 3005 3316 3663
Size: 1654 1351 311 347
Fragments arranged by size:
1654 1351 347 311
BsaAI yAC'GTr
Cuts at: 0 482 600 3663
Size: 482 118 3063
Fragments arranged by size:
3063 482 118
BsaHI Gr'CG_yC
Cuts at: 0 158 2318 3663
Size: 158 2160 1345
Fragments arranged by size:
2160 1345 158
BsaWI w'CCGG_w
Cuts at: 0 1396 1518 2295 3498 3663
Size: 1396 122 777 1203 165
Fragments arranged by size:
1396 1203 777 165 122
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 559 589 2770 2800 3174 3204
Size: 445 30 84 30 2181 30 374 30
Cuts at: 3204 3217 3247 3663
Size: 13 30 416
Fragments arranged by size:
2181 445 416 374 84 30 30 30
30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 450 453 637 1835 3037 3663
Size: 450 3 184 1198 1202 626
Fragments arranged by size:
1202 1198 626 450 184 3
BseYI C'CCAG_C
Cuts at: 0 2443 3345 3663
Size: 2443 902 318
Fragments arranged by size:
2443 902 318
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 307 439 1985 2662 3255 3663
Size: 274 33 132 1546 677 593 408
Fragments arranged by size:
1546 677 593 408 274 132 33
BsiEI CG_ry'CG
Cuts at: 0 125 265 3663
Size: 125 140 3398
Fragments arranged by size:
3398 140 125
BsiHKAI G_wGCw'C
Cuts at: 0 835 1243 1748 2656 2935 3406 3663
Size: 835 408 505 908 279 471 257
Fragments arranged by size:
908 835 505 471 408 279 257
BsmI GAATG_Cn'
Cuts at: 0 279 2913 3663
Size: 279 2634 750
Fragments arranged by size:
2634 750 279
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2509 2664 3663
Size: 2509 155 999
Fragments arranged by size:
2509 999 155
Bsp1286I G_dGCh'C
Cuts at: 0 52 835 1243 1319 1493 1748 1754 1956
Size: 52 783 408 76 174 255 6 202
Cuts at: 1956 2507 2656 2768 2935 3061 3406 3663
Size: 551 149 112 167 126 345 257
Fragments arranged by size:
783 551 408 345 257 255 202 174
167 149 126 112 76 52 6
BspEI T'CCGG_A
Cuts at: 0 1518 3663
Size: 1518 2145
BspHI T'CATG_A
Cuts at: 0 1305 2178 2588 3663
Size: 1305 873 410 1075
Fragments arranged by size:
1305 1075 873 410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2337 2628 3334 3663
Size: 2337 291 706 329
Fragments arranged by size:
2337 706 329 291
BsrDI GCAATG_nn'
Cuts at: 0 430 1935 2093 3385 3663
Size: 430 1505 158 1292 278
Fragments arranged by size:
1505 1292 430 278 158
BsrFI r'CCGG_y
Cuts at: 0 3155 3461 3663
Size: 3155 306 202
Fragments arranged by size:
3155 306 202
BsrGI T'GTAC_A
Cuts at: 0 605 3359 3663
Size: 605 2754 304
Fragments arranged by size:
2754 605 304
BssSI C'ACGA_G
Cuts at: 0 892 3663
Size: 892 2771
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3647 3663
Size: 3647 16
BstBI TT'CG_AA
Cuts at: 0 2373 3663
Size: 2373 1290
BstEII G'GTnAC_C
Cuts at: 0 586 3663
Size: 586 3077
BstXI CCAn_nnnn'nTGG
Cuts at: 0 379 2816 3663
Size: 379 2437 847
Fragments arranged by size:
2437 847 379
BstYI r'GATC_y
Cuts at: 0 629 2864 3552 3663
Size: 629 2235 688 111
Fragments arranged by size:
2235 688 629 111
Bsu36I CC'TnA_GG
Cuts at: 0 1466 3663
Size: 1466 2197
BtgI C'CryG_G
Cuts at: 0 128 642 1764 1989 3391 3663
Size: 128 514 1122 225 1402 272
Fragments arranged by size:
1402 1122 514 272 225 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1284 3663
Size: 1284 2379
BtsI GCAGTG_nn'
Cuts at: 0 1842 2745 2912 3406 3663
Size: 1842 903 167 494 257
Fragments arranged by size:
1842 903 494 257 167
ClaI AT'CG_AT
Cuts at: 0 2040 3663
Size: 2040 1623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3000 3035 3663
Size: 3000 35 628
Fragments arranged by size:
3000 628 35
DraI TTT'AAA
Cuts at: 0 987 3663
Size: 987 2676
DraIII CAC_nnn'GTG
Cuts at: 0 1559 3663
Size: 1559 2104
EaeI y'GGCC_r
Cuts at: 0 83 660 1767 3663
Size: 83 577 1107 1896
Fragments arranged by size:
1896 1107 577 83
EarI CTCTTCn'nnn_
Cuts at: 0 2024 3214 3314 3663
Size: 2024 1190 100 349
Fragments arranged by size:
2024 1190 349 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1767 3663
Size: 1767 1896
EcoICRI GAG'CTC
Cuts at: 0 833 1746 3663
Size: 833 913 1917
Fragments arranged by size:
1917 913 833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 380 1076 1388 2340 2430 2652 2864
Size: 79 301 696 312 952 90 222 212
Cuts at: 2864 3663
Size: 799
Fragments arranged by size:
952 799 696 312 301 222 212 90
79
EcoNI CCTnn'n_nnAGG
Cuts at: 0 810 3052 3663
Size: 810 2242 611
Fragments arranged by size:
2242 810 611
EcoO109I rG'GnC_Cy
Cuts at: 0 60 873 1751 2503 3663
Size: 60 813 878 752 1160
Fragments arranged by size:
1160 878 813 752 60
EcoRI G'AATT_C
Cuts at: 0 523 3663
Size: 523 3140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2904 3663
Size: 2904 759
FseI GG_CCGG'CC
Cuts at: 0 3465 3663
Size: 3465 198
HaeII r_GCGC'y
Cuts at: 0 94 161 2321 2469 3663
Size: 94 67 2160 148 1194
Fragments arranged by size:
2160 1194 148 94 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 711 743 1052 1059 1084 1091
Size: 445 32 234 32 309 7 25 7
Cuts at: 1091 1134 1166 3537 3541 3569 3573 3663
Size: 43 32 2371 4 28 4 90
Fragments arranged by size:
2371 445 309 234 90 43 32 32
32 28 25 7 7 4 4
KasI G'GCGC_C
Cuts at: 0 157 2317 3663
Size: 157 2160 1346
Fragments arranged by size:
2160 1346 157
KpnI G_GTAC'C
Cuts at: 0 642 3663
Size: 642 3021
MscI TGG'CCA
Cuts at: 0 85 3663
Size: 85 3578
MslI CAynn'nnrTG
Cuts at: 0 758 961 1015 1151 1632 1655 3390 3663
Size: 758 203 54 136 481 23 1735 273
Fragments arranged by size:
1735 758 481 273 203 136 54 23
MspA1I CmG'CkG
Cuts at: 0 99 130 155 164 636 856 1908 2314
Size: 99 31 25 9 472 220 1052 406
Cuts at: 2314 3663
Size: 1349
Fragments arranged by size:
1349 1052 472 406 220 99 31 25
9
NaeI GCC'GGC
Cuts at: 0 3157 3463 3663
Size: 3157 306 200
Fragments arranged by size:
3157 306 200
NarI GG'CG_CC
Cuts at: 0 158 2318 3663
Size: 158 2160 1345
Fragments arranged by size:
2160 1345 158
NgoMIV G'CCGG_C
Cuts at: 0 3155 3461 3663
Size: 3155 306 202
Fragments arranged by size:
3155 306 202
NsiI A_TGCA'T
Cuts at: 0 705 3663
Size: 705 2958
NspI r_CATG'y
Cuts at: 0 1814 2828 3663
Size: 1814 1014 835
Fragments arranged by size:
1814 1014 835
PciI A'CATG_T
Cuts at: 0 1810 3663
Size: 1810 1853
PfoI T'CCnGG_A
Cuts at: 0 1804 1832 2336 3663
Size: 1804 28 504 1327
Fragments arranged by size:
1804 1327 504 28
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3174 3206 3663
Size: 3174 32 457
Fragments arranged by size:
3174 457 32
PshAI GACnn'nnGTC
Cuts at: 0 2521 2997 3663
Size: 2521 476 666
Fragments arranged by size:
2521 666 476
PsiI TTA'TAA
Cuts at: 0 2252 3663
Size: 2252 1411
PspOMI G'GGCC_C
Cuts at: 0 48 1750 2503 3663
Size: 48 1702 753 1160
Fragments arranged by size:
1702 1160 753 48
PstI C_TGCA'G
Cuts at: 0 1360 1432 2348 2740 3119 3663
Size: 1360 72 916 392 379 544
Fragments arranged by size:
1360 916 544 392 379 72
PvuI CG_AT'CG
Cuts at: 0 265 3663
Size: 265 3398
PvuII CAG'CTG
Cuts at: 0 99 856 1908 2314 3663
Size: 99 757 1052 406 1349
Fragments arranged by size:
1349 1052 757 406 99
SacI G_AGCT'C
Cuts at: 0 835 1748 3663
Size: 835 913 1915
Fragments arranged by size:
1915 913 835
SacII CC_GC'GG
Cuts at: 0 131 3663
Size: 131 3532
SfcI C'TryA_G
Cuts at: 0 570 1356 1428 2344 2736 3115 3160 3663
Size: 570 786 72 916 392 379 45 503
Fragments arranged by size:
916 786 570 503 392 379 72 45
SfoI GGC'GCC
Cuts at: 0 159 2319 3663
Size: 159 2160 1344
Fragments arranged by size:
2160 1344 159
SmlI C'TyrA_G
Cuts at: 0 334 1444 1701 2717 3471 3663
Size: 334 1110 257 1016 754 192
Fragments arranged by size:
1110 1016 754 334 257 192
SpeI A'CTAG_T
Cuts at: 0 2163 3663
Size: 2163 1500
SspI AAT'ATT
Cuts at: 0 1917 2290 3294 3663
Size: 1917 373 1004 369
Fragments arranged by size:
1917 1004 373 369
StuI AGG'CCT
Cuts at: 0 2258 2836 3663
Size: 2258 578 827
Fragments arranged by size:
2258 827 578
StyI C'CwwG_G
Cuts at: 0 86 1599 2582 2988 3663
Size: 86 1513 983 406 675
Fragments arranged by size:
1513 983 675 406 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 111 2952 3199 3663
Size: 111 2841 247 464
Fragments arranged by size:
2841 464 247 111
TatI w'GTAC_w
Cuts at: 0 605 2051 2821 3359 3663
Size: 605 1446 770 538 304
Fragments arranged by size:
1446 770 605 538 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1680 1712 1800 1832 2796 2828 3014 3046
Size: 1680 32 88 32 964 32 186 32
Cuts at: 3046 3217 3249 3663
Size: 171 32 414
Fragments arranged by size:
1680 964 414 186 171 88 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3435 3663
Size: 3435 228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 640 2561 3663
Size: 640 1921 1102
Fragments arranged by size:
1921 1102 640
XmnI GAAnn'nnTTC
Cuts at: 0 2887 3247 3663
Size: 2887 360 416
Fragments arranged by size:
2887 416 360
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BclI BfrBI BglI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII
Hin4I KasI KpnI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PciI PfoI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SfcI SfoI
SmlI SpeI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AgeI AhdI AlfI AscI AseI
AsiSI AvrII BaeI BciVI BglII BlpI BmgBI BmtI
BplI BsaBI BsiWI BsrBI BssHII BstZ17I DrdI EagI
EcoRV FspI FspAI HincII HindIII HpaI MfeI MluI
MmeI NcoI NdeI NheI NotI NruI PacI PasI
PflMI PmeI PmlI PpuMI PspXI PsrI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SphI SrfI SwaI XbaI XhoI XmaI
ZraI