Restriction map for
pFN21AB9484
##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp718I
AvrII BamHI BfrI BglII BlnI
BsaBI BsiWI BsmBI BspDI BspHI
BspLU11I Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eco47III EcoRV EcoT22I
HindIII HpaI KpnI MamI MfeI
MluI MseI MunI NdeI NheI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I RcaI RsrII SacI
SalI ScaI SfuI SgrAI SpeI
SspI SwaI Tru9I XbaI
###################################
# ONE CUT ENZYMES ( 14 enzymes) #
###################################
AviII 5690 TGCGCA TGC^GCA
BbvCI 1781 CCTCAGC CCTCAGC(-5/-2)
BciVI 5592 GGATAC GTATCC(6/5)
BclI 278 TGATCA T^GATCA
BfaI 4276 CTAG C^TAG
DrdI 1075 GACGACGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 278 TGATCA T^GATCA
FseI 3 GGCCGGCC GGCCGG^CC
FspI 5690 TGCGCA TGC^GCA
MaeI 4276 CTAG C^TAG
NruI 3064 TCGCGA TCG^CGA
PvuI 1114 CGATCG CGAT^CG
SfiI 5014 GGCCCCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1733 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 3534 GAATTC [GA]^AATT[CT]
4195 GAATTC
Alw44I 1744 GTGCAC G^TGCAC
3616 GTGCAC
ApaLI 1744 GTGCAC G^TGCAC
3616 GTGCAC
ApoI 3534 GAATTC [GA]^AATT[CT]
4195 GAATTC
Asp700I 921 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3549 GAAGGATTTC
BlpI 708 GCTCAGC GC^T[ACGT]AGC
2750 GCTCAGC
Bpu1102I 708 GCTCAGC GC^T[ACGT]AGC
2750 GCTCAGC
Bsp1407I 2530 TGTACA T^GTACA
4229 TGTACA
BsrGI 2530 TGTACA T^GTACA
4229 TGTACA
BstEII 2166 GGTCACC G^GT[ACGT]ACC
5443 GGTCACC
BstPI 2166 GGTCACC G^GT[ACGT]ACC
5443 GGTCACC
CelII 708 GCTCAGC GC^T[ACGT]AGC
2750 GCTCAGC
DraIII 2178 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3099 CACCGTGTG
EcoO65I 2166 GGTCACC G^GT[ACGT]ACC
5443 GGTCACC
EcoRI 3534 GAATTC G^AATTC
4195 GAATTC
HincII 4573 GTCAAC GT[CT]^[GA]AC
5839 GTCAAC
HindII 4573 GTCAAC GT[CT]^[GA]AC
5839 GTCAAC
KspI 714 CCGCGG CCGC^GG
2663 CCGCGG
NotI 30 GCGGCCGC GC^GGCCGC
3714 GCGGCCGC
NspI 88 ACATGC [GA]CATG^[CT]
1733 GCATGC
PaeR7I 1482 CTCGAG C^TCGAG
3253 CTCGAG
SacII 714 CCGCGG CCGC^GG
2663 CCGCGG
SapI 594 GAAGAGC GCTCTTC(1/4)
4764 GAAGAGC
SbfI 1470 CCTGCAGG CCTGCA^GG
5895 CCTGCAGG
SexAI 233 ACCTGGT A^CC[AT]GGT
314 ACCAGGT
SmlI 1482 CTCGAG C^T[CT][GA]AG
3253 CTCGAG
SnaBI 2227 TACGTA TAC^GTA
5290 TACGTA
Sse8387I 1470 CCTGCAGG CCTGCA^GG
5895 CCTGCAGG
SspBI 2530 TGTACA T^GTACA
4229 TGTACA
StuI 4947 AGGCCT AGG^CCT
6020 AGGCCT
TfiI 3175 GAATC G^A[AT]TC
5537 GATTC
Tsp509I 3535 AATT ^AATT
4196 AATT
XhoI 1482 CTCGAG C^TCGAG
3253 CTCGAG
XmnI 921 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3549 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AatII AccII AccIII AciI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI AspEI AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvI
BcgI BcnI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflFI PflMI PleI
PmaCI PmlI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StyI
TaqI TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI