(Circular) (Six-base) MAPSORT of: pFN21AB9484.seq Check: 9441 from: 1 to: 10840 pFN21AB9484 10840 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6074 7414 7794 7894 8097 6074 Size: 1340 380 100 203 8817 Fragments arranged by size: 8817 1340 380 203 100 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5550 5968 6579 7434 Size: 53 83 186 936 4014 418 611 855 Cuts at: 7434 8650 278 Size: 1216 2468 Fragments arranged by size: 4014 2468 1216 936 855 611 418 186 83 53 AccI GT'mk_AC Cuts at: 1937 8183 1937 Size: 6246 4594 Acc65I G'GTAC_C Cuts at: 8161 8161 Size: 10840 AclI AA'CG_TT Cuts at: 1563 8967 9340 1563 Size: 7404 373 3063 Fragments arranged by size: 7404 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4108 4217 4874 5629 7300 7301 8903 10287 Size: 2476 109 657 755 1671 1 1602 1384 Cuts at: 10287 10555 1632 Size: 268 1917 Fragments arranged by size: 2476 1917 1671 1602 1384 755 657 268 109 1 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10643 AflIII A'CryG_T Cuts at: 1203 2190 3437 3720 5924 9739 10716 1203 Size: 987 1247 283 2204 3815 977 1327 Fragments arranged by size: 3815 2204 1327 1247 987 977 283 AgeI A'CCGG_T Cuts at: 9671 9671 Size: 10840 AhdI GACnn_n'nnGTC Cuts at: 5476 7075 7435 9569 5476 Size: 1599 360 2134 6747 Fragments arranged by size: 6747 2134 1599 360 AleI CACnn'nnGTG Cuts at: 1166 2492 5399 1166 Size: 1326 2907 6607 Fragments arranged by size: 6607 2907 1326 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2726 3372 4009 6584 6981 1184 Size: 1542 646 637 2575 397 5043 Fragments arranged by size: 5043 2575 1542 646 637 397 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2438 2470 6989 7021 2438 Size: 32 4519 32 6257 Fragments arranged by size: 6257 4519 32 32 AlwNI CAG_nnn'CTG Cuts at: 2778 2899 3930 4060 4380 4879 4900 4993 5446 Size: 121 1031 130 320 499 21 93 453 Cuts at: 5446 6226 6592 6703 7399 7717 8583 10155 2778 Size: 780 366 111 696 318 866 1572 3463 Fragments arranged by size: 3463 1572 1031 866 780 696 499 453 366 320 318 130 121 111 93 21 ApaI G_GGCC'C Cuts at: 2030 3383 4667 4907 5592 5671 5869 6691 6909 Size: 1353 1284 240 685 79 198 822 218 Cuts at: 6909 8012 2030 Size: 1103 4858 Fragments arranged by size: 4858 1353 1284 1103 822 685 240 218 198 79 ApaLI G'TGCA_C Cuts at: 3749 5621 8897 10053 3749 Size: 1872 3276 1156 4536 Fragments arranged by size: 4536 3276 1872 1156 ApoI r'AATT_y Cuts at: 1493 5539 6200 8149 8375 8527 10636 1493 Size: 4046 661 1949 226 152 2109 1697 Fragments arranged by size: 4046 2109 1949 1697 661 226 152 AseI AT'TA_AT Cuts at: 160 1032 9394 160 Size: 872 8362 1606 Fragments arranged by size: 8362 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10840 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2247 2474 2843 2983 3041 3132 Size: 73 80 224 227 369 140 58 91 Cuts at: 3132 3410 3487 3569 3981 4347 4613 5258 5459 Size: 278 77 82 412 366 266 645 201 Cuts at: 5459 5664 5862 6249 6500 6624 6824 6886 7461 Size: 205 198 387 251 124 200 62 575 Cuts at: 7461 7504 7535 8165 1870 Size: 43 31 630 4545 Fragments arranged by size: 4545 645 630 575 412 387 369 366 278 266 251 227 224 205 201 200 198 140 124 91 82 80 77 73 62 58 43 31 BamHI G'GATC_C Cuts at: 8170 8537 8170 Size: 367 10473 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2543 2623 2837 2870 2969 Size: 325 217 627 756 80 214 33 99 Cuts at: 2969 3018 3064 4412 4562 5009 5125 5137 5650 Size: 49 46 1348 150 447 116 12 513 Cuts at: 5650 5697 6092 6116 6366 6393 6401 6452 6542 Size: 47 395 24 250 27 8 51 90 Cuts at: 6542 6805 6866 7211 7361 7782 7946 8076 8161 Size: 263 61 345 150 421 164 130 85 Cuts at: 8161 9616 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1348 756 627 513 447 421 395 345 325 263 250 217 214 164 150 150 130 116 99 90 85 80 61 51 49 47 46 33 27 24 12 8 BanII G_rGCy'C Cuts at: 1391 2030 2559 2606 3198 3383 3637 4230 4667 Size: 639 529 47 592 185 254 593 437 Cuts at: 4667 4907 4977 5259 5265 5381 5592 5671 5869 Size: 240 70 282 6 116 211 79 198 Cuts at: 5869 6691 6776 6909 7811 7975 8012 8159 1391 Size: 822 85 133 902 164 37 147 4072 Fragments arranged by size: 4072 902 822 639 593 592 529 437 282 254 240 211 198 185 164 147 133 116 85 79 70 47 37 6 BbeI G_GCGC'C Cuts at: 2547 2627 2841 2874 2973 3022 3068 4566 5013 Size: 80 214 33 99 49 46 1498 447 Cuts at: 5013 5701 6096 6120 6397 6546 6809 7950 2547 Size: 688 395 24 277 149 263 1141 5437 Fragments arranged by size: 5437 1498 1141 688 447 395 277 263 214 149 99 80 49 46 33 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2923 4862 5360 7058 928 Size: 971 1024 1939 498 1698 4710 Fragments arranged by size: 4710 1939 1698 1024 971 498 BbvCI CC'TCA_GC Cuts at: 3787 3787 Size: 10840 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2122 2156 3168 3202 6815 6849 9031 Size: 34 185 34 1012 34 3613 34 2182 Cuts at: 9031 9065 1903 Size: 34 3678 Fragments arranged by size: 3678 3613 2182 1012 185 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7591 8732 9942 7591 Size: 1141 1210 8489 Fragments arranged by size: 8489 1210 1141 BclI T'GATC_A Cuts at: 1548 2283 1548 Size: 735 10105 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3062 4365 4530 Size: 107 122 71 631 605 1390 1303 165 Cuts at: 4530 4671 4875 5648 6081 6223 6738 7026 9451 Size: 141 204 773 433 142 515 288 2425 Cuts at: 9451 136 Size: 1525 Fragments arranged by size: 2425 1525 1390 1303 773 631 605 515 433 288 204 165 142 141 122 107 71 BglII A'GATC_T Cuts at: 10835 10835 Size: 10840 BlpI GC'TnA_GC Cuts at: 2714 4756 8257 2714 Size: 2042 3501 5297 Fragments arranged by size: 5297 3501 2042 Bme1580I G_kGCm'C Cuts at: 1790 2030 2612 2822 3191 3295 3383 3671 3720 Size: 240 582 210 369 104 88 288 49 Cuts at: 3720 3753 4306 4660 4667 4907 5130 5592 5625 Size: 33 553 354 7 240 223 462 33 Cuts at: 5625 5653 5671 5869 5961 6404 6691 6734 6851 Size: 28 18 198 92 443 287 43 117 Cuts at: 6851 6869 6909 7216 7785 7842 8012 8901 10057 Size: 18 40 307 569 57 170 889 1156 Cuts at: 10057 1790 Size: 2573 Fragments arranged by size: 2573 1156 889 582 569 553 462 443 369 354 307 288 287 240 240 223 210 198 170 117 104 92 88 57 49 43 40 33 33 28 18 18 7 BmgBI CAC'GTC Cuts at: 1318 3438 5659 5787 5927 7437 7594 1318 Size: 2120 2221 128 140 1510 157 4564 Fragments arranged by size: 4564 2221 2120 1510 157 140 128 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2340 4368 5871 Size: 416 53 83 399 24 923 2028 1503 Cuts at: 5871 6052 6441 8132 8571 9529 442 Size: 181 389 1691 439 958 1753 Fragments arranged by size: 2028 1753 1691 1503 958 923 439 416 399 389 181 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10840 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2321 4771 6160 2321 Size: 2450 1389 7001 Fragments arranged by size: 7001 2450 1389 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2395 2462 2475 3569 3941 5438 5444 Size: 893 263 67 13 1094 372 1497 6 Cuts at: 5444 6233 6395 6545 6614 6725 6962 7016 7124 Size: 789 162 150 69 111 237 54 108 Cuts at: 7124 7211 7747 8114 9500 1239 Size: 87 536 367 1386 2579 Fragments arranged by size: 2579 1497 1386 1094 893 789 536 372 367 263 237 162 150 111 108 87 69 67 54 13 6 Bpu10I CC'TnA_GC Cuts at: 3787 4982 6299 3787 Size: 1195 1317 8328 Fragments arranged by size: 8328 1317 1195 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 8965 9830 10128 8965 Size: 865 298 9677 Fragments arranged by size: 9677 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2292 3143 3174 3644 3797 6416 7189 Size: 617 793 851 31 470 153 2619 773 Cuts at: 7189 7381 9503 882 Size: 192 2122 2219 Fragments arranged by size: 2619 2219 2122 851 793 773 617 470 192 153 31 BsaAI yAC'GTr Cuts at: 493 1204 2193 3309 3723 3748 3781 4234 5737 Size: 711 989 1116 414 25 33 453 1503 Cuts at: 5737 7297 7414 8631 493 Size: 1560 117 1217 2702 Fragments arranged by size: 2702 1560 1503 1217 1116 989 711 453 414 117 33 25 BsaBI GATnn'nnATC Cuts at: 10834 10834 Size: 10840 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2504 2544 2582 2624 Size: 53 83 186 936 971 40 38 42 Cuts at: 2624 2643 2838 2871 2970 3019 3065 3522 4310 Size: 19 195 33 99 49 46 457 788 Cuts at: 4310 4563 5010 5165 5357 5412 5430 5547 5698 Size: 253 447 155 192 55 18 117 151 Cuts at: 5698 5965 6093 6117 6394 6543 6576 6806 7431 Size: 267 128 24 277 149 33 230 625 Cuts at: 7431 7947 8100 8647 9029 9664 275 Size: 516 153 547 382 635 1451 Fragments arranged by size: 1451 971 936 788 635 625 547 516 457 447 382 277 267 253 230 195 192 186 155 153 151 149 128 117 99 83 55 53 49 46 42 40 38 33 33 24 19 18 BsaWI w'CCGG_w Cuts at: 4016 5535 5562 9273 9671 9945 10092 10503 4016 Size: 1519 27 3711 398 274 147 411 4353 Fragments arranged by size: 4353 3711 1519 411 398 274 147 27 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 2438 2468 2732 Size: 30 165 30 777 30 228 30 264 Cuts at: 2732 2762 3804 3834 4519 4549 5041 5071 6081 Size: 30 1042 30 685 30 492 30 1010 Cuts at: 6081 6111 6917 6947 6989 7019 7793 7823 1178 Size: 30 806 30 42 30 774 30 4195 Fragments arranged by size: 4195 1042 1010 806 777 774 685 492 264 228 165 42 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2141 2174 2459 3131 3989 4760 4967 Size: 952 3 33 285 672 858 771 207 Cuts at: 4967 7924 1186 Size: 2957 4102 Fragments arranged by size: 4102 2957 952 858 771 672 285 207 33 3 BseYI C'CCAG_C Cuts at: 2612 3365 3693 3728 4423 4928 5027 5153 5948 Size: 753 328 35 695 505 99 126 795 Cuts at: 5948 5982 7022 7812 7942 8011 10043 10539 2612 Size: 34 1040 790 130 69 2032 496 2913 Fragments arranged by size: 2913 2032 1040 795 790 753 695 505 496 328 130 126 99 69 35 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 2346 2707 3437 3728 3742 3761 Size: 573 468 142 361 730 291 14 19 Cuts at: 3761 5133 6314 6941 7441 8069 1163 Size: 1372 1181 627 500 628 3934 Fragments arranged by size: 3934 1372 1181 730 628 627 573 500 468 361 291 142 19 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2014 2039 2793 3122 3256 4654 Size: 275 186 13 25 754 329 134 1398 Cuts at: 4654 5723 6742 7204 8290 9051 10079 1540 Size: 1069 1019 462 1086 761 1028 2301 Fragments arranged by size: 2301 1398 1086 1069 1028 1019 761 754 462 329 275 186 134 25 13 BsiHKAI G_wGCw'C Cuts at: 2145 3488 3753 3845 3879 4442 4604 5519 5625 Size: 1343 265 92 34 563 162 915 106 Cuts at: 5625 5736 6838 7413 7725 8901 8986 10057 2145 Size: 111 1102 575 312 1176 85 1071 2928 Fragments arranged by size: 2928 1343 1176 1102 1071 915 575 563 312 265 162 111 106 92 85 34 BsmI GAATG_Cn' Cuts at: 2532 2669 3476 3683 8351 8444 2532 Size: 137 807 207 4668 93 4928 Fragments arranged by size: 4928 4668 807 207 137 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2145 2559 2606 2612 2822 3191 Size: 399 240 115 414 47 6 210 369 Cuts at: 3191 3198 3295 3383 3488 3637 3671 3720 3753 Size: 7 97 88 105 149 34 49 33 Cuts at: 3753 3845 3879 4230 4306 4442 4604 4660 4667 Size: 92 34 351 76 136 162 56 7 Cuts at: 4667 4907 4977 5130 5259 5265 5381 5519 5592 Size: 240 70 153 129 6 116 138 73 Cuts at: 5592 5625 5653 5671 5736 5869 5961 6404 6691 Size: 33 28 18 65 133 92 443 287 Cuts at: 6691 6734 6776 6838 6851 6869 6909 7216 7413 Size: 43 42 62 13 18 40 307 197 Cuts at: 7413 7725 7785 7811 7842 7975 8012 8159 8901 Size: 312 60 26 31 133 37 147 742 Cuts at: 8901 8986 10057 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 414 399 369 351 312 307 287 240 240 210 197 162 153 149 147 138 136 133 133 129 116 115 105 97 92 92 88 85 76 73 70 65 62 60 56 49 47 43 42 40 37 34 34 33 33 31 28 26 18 18 13 7 7 6 6 BspEI T'CCGG_A Cuts at: 4016 5535 5562 10503 4016 Size: 1519 27 4941 4353 Fragments arranged by size: 4941 4353 1519 27 BspHI T'CATG_A Cuts at: 8729 10459 8729 Size: 1730 9110 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2768 2837 6041 6074 7414 7436 7794 7894 Size: 1924 69 3204 33 1340 22 358 100 Cuts at: 7894 8097 8195 844 Size: 203 98 3489 Fragments arranged by size: 3489 3204 1924 1340 358 203 100 98 69 33 22 BsrBI CCG'CTC Cuts at: 1394 1823 2050 2116 5220 6144 6529 7291 7747 Size: 429 227 66 3104 924 385 762 456 Cuts at: 7747 8727 1394 Size: 980 3507 Fragments arranged by size: 3507 3104 980 924 762 456 429 385 227 66 BsrDI GCAATG_nn' Cuts at: 65 1248 5796 6721 7918 9335 9509 65 Size: 1183 4548 925 1197 1417 174 1396 Fragments arranged by size: 4548 1417 1396 1197 1183 925 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2013 2084 3201 3345 4065 4996 Size: 110 199 4 71 1117 144 720 931 Cuts at: 4996 5700 6127 6322 6346 6545 7928 8073 9484 Size: 704 427 195 24 199 1383 145 1411 Cuts at: 9484 9671 1700 Size: 187 2869 Fragments arranged by size: 2869 1411 1383 1117 931 720 704 427 199 199 195 187 145 144 110 71 24 4 BsrGI T'GTAC_A Cuts at: 96 4535 6234 96 Size: 4439 1699 4702 Fragments arranged by size: 4702 4439 1699 BssHII G'CGCG_C Cuts at: 1925 2104 2106 2783 3590 1925 Size: 179 2 677 807 9175 Fragments arranged by size: 9175 807 677 179 2 BssSI C'ACGA_G Cuts at: 2809 4603 7014 8900 9912 2809 Size: 1794 2411 1886 1012 3737 Fragments arranged by size: 3737 2411 1886 1794 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2664 3154 3540 3930 6873 7417 8083 794 Size: 1870 490 386 390 2943 544 666 3551 Fragments arranged by size: 3551 2943 1870 666 544 490 390 386 BstBI TT'CG_AA Cuts at: 9644 9644 Size: 10840 BstEII G'GTnAC_C Cuts at: 1184 4171 7448 1184 Size: 2987 3277 4576 Fragments arranged by size: 4576 3277 2987 BstXI CCAn_nnnn'nTGG Cuts at: 2376 2620 5161 7372 7957 2376 Size: 244 2541 2211 585 5259 Fragments arranged by size: 5259 2541 2211 585 244 BstYI r'GATC_y Cuts at: 1972 2163 2343 3579 5682 8170 8537 8922 8939 Size: 191 180 1236 2103 2488 367 385 17 Cuts at: 8939 10391 10477 10489 10835 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2488 2103 1977 1452 1236 385 367 346 191 180 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4466 6219 6551 7468 4466 Size: 1753 332 917 7838 Fragments arranged by size: 7838 1753 917 332 BtgI C'CryG_G Cuts at: 513 1065 2004 2719 2833 3229 3555 4396 4668 Size: 552 939 715 114 396 326 841 272 Cuts at: 4668 5133 5811 5907 6022 6516 6679 6796 7740 Size: 465 678 96 115 494 163 117 944 Cuts at: 7740 7818 8004 513 Size: 78 186 3349 Fragments arranged by size: 3349 944 939 841 715 678 552 494 465 396 326 272 186 163 117 115 114 96 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4846 5052 5220 5817 487 Size: 683 56 3620 206 168 597 5510 Fragments arranged by size: 5510 3620 683 597 206 168 56 BtsI GCAGTG_nn' Cuts at: 2690 3425 3626 3860 3931 4646 4991 5082 5826 Size: 735 201 234 71 715 345 91 744 Cuts at: 5826 5940 7085 7555 7664 8015 8356 9150 9170 Size: 114 1145 470 109 351 341 794 20 Cuts at: 9170 2690 Size: 4360 Fragments arranged by size: 4360 1145 794 744 735 715 470 351 345 341 234 201 114 109 91 71 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10840 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4795 4830 529 Size: 35 4231 35 6539 Fragments arranged by size: 6539 4231 35 35 DraI TTT'AAA Cuts at: 8144 8144 Size: 10840 DraIII CAC_nnn'GTG Cuts at: 4188 5109 4188 Size: 921 9919 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3086 9847 809 Size: 507 1770 6761 1802 Fragments arranged by size: 6761 1802 1770 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2011 2036 Size: 54 1425 50 275 18 177 4 25 Cuts at: 2036 2061 2378 2790 3163 3232 4522 4651 5283 Size: 25 317 412 373 69 1290 129 632 Cuts at: 5283 5720 5814 6286 6739 7201 7767 8287 9176 Size: 437 94 472 453 462 566 520 889 Cuts at: 9176 10805 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1290 889 632 566 520 472 462 453 437 412 373 317 275 177 129 94 69 54 50 43 25 25 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2011 2036 2790 4651 5720 6739 7201 Size: 275 199 25 754 1861 1069 1019 462 Cuts at: 7201 8287 1537 Size: 1086 4090 Fragments arranged by size: 4090 1861 1086 1069 1019 754 462 275 199 25 EarI CTCTTCn'nnn_ Cuts at: 1353 2594 5537 6764 8770 1353 Size: 1241 2943 1227 2006 3423 Fragments arranged by size: 3423 2943 2006 1241 1227 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2696 5049 5158 7633 9427 9801 Size: 1064 138 1107 2353 109 2475 1794 374 Cuts at: 9801 9947 387 Size: 146 1280 Fragments arranged by size: 2475 2353 1794 1280 1107 1064 374 146 138 109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2395 2462 2475 3569 3941 4108 Size: 393 500 263 67 13 1094 372 167 Cuts at: 4108 4217 4874 5438 5444 5629 6233 6395 6545 Size: 109 657 564 6 185 604 162 150 Cuts at: 6545 6614 6725 6962 7016 7124 7211 7300 7301 Size: 69 111 237 54 108 87 89 1 Cuts at: 7301 7747 8114 8903 9500 10287 10555 1239 Size: 446 367 789 597 787 268 1524 Fragments arranged by size: 1524 1094 789 787 657 604 597 564 500 446 393 372 367 268 263 237 185 167 162 150 111 109 108 89 87 69 67 54 13 6 1 EcoNI CCTnn'n_nnAGG Cuts at: 2444 3641 4985 7124 7177 8093 2444 Size: 1197 1344 2139 53 916 5191 Fragments arranged by size: 5191 2139 1344 1197 916 53 EcoO109I rG'GnC_Cy Cuts at: 2478 2740 3273 3379 3394 3599 4259 4462 4664 Size: 262 533 106 15 205 660 203 202 Cuts at: 4664 4876 5581 5588 5668 5866 6030 6687 6688 Size: 212 705 7 80 198 164 657 1 Cuts at: 6688 6906 6973 7019 7491 7918 7987 2478 Size: 218 67 46 472 427 69 5331 Fragments arranged by size: 5331 705 660 657 533 472 427 262 218 212 205 203 202 198 164 106 80 69 67 46 15 7 1 EcoRI G'AATT_C Cuts at: 5539 6200 8149 8527 5539 Size: 661 1949 378 7852 Fragments arranged by size: 7852 1949 661 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 7087 7087 Size: 10840 FseI GG_CCGG'CC Cuts at: 2013 2013 Size: 10840 FspI TGC'GCA Cuts at: 7697 9346 7697 Size: 1649 9191 HaeII r_GCGC'y Cuts at: 1678 2547 2627 2701 2841 2874 2973 3022 3068 Size: 869 80 74 140 33 99 49 46 Cuts at: 3068 3661 4566 5013 5701 6096 6120 6212 6278 Size: 593 905 447 688 395 24 92 66 Cuts at: 6278 6397 6524 6546 6592 6809 6995 7308 7950 Size: 119 127 22 46 217 186 313 642 Cuts at: 7950 9987 1678 Size: 2037 2531 Fragments arranged by size: 2531 2037 905 869 688 642 593 447 395 313 217 186 140 127 119 99 92 80 74 66 49 46 46 33 24 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2732 2764 4079 4111 4497 Size: 32 135 32 1160 32 1315 32 386 Cuts at: 4497 4529 5041 5073 5462 5494 6081 6113 7061 Size: 32 512 32 389 32 587 32 948 Cuts at: 7061 7093 7421 7453 9481 9513 9555 9587 1373 Size: 32 328 32 2028 32 42 32 2626 Fragments arranged by size: 2626 2028 1315 1160 948 587 512 389 386 328 135 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6580 7846 8184 8430 669 Size: 988 281 4642 1266 338 246 3079 Fragments arranged by size: 4642 3079 1266 988 338 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 10840 HpaI GTT'AAC Cuts at: 8430 8430 Size: 10840 KasI G'GCGC_C Cuts at: 2543 2623 2837 2870 2969 3018 3064 4562 5009 Size: 80 214 33 99 49 46 1498 447 Cuts at: 5009 5697 6092 6116 6393 6542 6805 7946 2543 Size: 688 395 24 277 149 263 1141 5437 Fragments arranged by size: 5437 1498 1141 688 447 395 277 263 214 149 99 80 49 46 33 24 KpnI G_GTAC'C Cuts at: 8165 8165 Size: 10840 MfeI C'AATT_G Cuts at: 8439 8439 Size: 10840 MluI A'CGCG_T Cuts at: 10716 10716 Size: 10840 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2525 2619 2775 3735 5406 6727 9954 10138 Size: 1220 94 156 960 1671 1321 3227 184 Cuts at: 10138 1305 Size: 2007 Fragments arranged by size: 3227 2007 1671 1321 1220 960 184 156 94 MscI TGG'CCA Cuts at: 10 64 1489 1832 2380 5285 7769 10 Size: 54 1425 343 548 2905 2484 3081 Fragments arranged by size: 3081 2905 2484 1425 548 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2374 2492 5399 8798 9157 9316 518 Size: 648 1208 118 2907 3399 359 159 2042 Fragments arranged by size: 3399 2907 2042 1208 648 359 159 118 MspA1I CmG'CkG Cuts at: 1606 2032 2041 2289 2677 2721 2994 3243 3612 Size: 426 9 248 388 44 273 249 369 Cuts at: 3612 3791 4128 4276 4479 4548 4670 4852 5725 Size: 179 337 148 203 69 122 182 873 Cuts at: 5725 6072 7026 7136 7372 7396 7586 7857 7942 Size: 347 954 110 236 24 190 271 85 Cuts at: 7942 7957 8083 8256 8933 10081 10326 1606 Size: 15 126 173 677 1148 245 2120 Fragments arranged by size: 2120 1148 954 873 677 426 388 369 347 337 273 271 249 248 245 236 203 190 182 179 173 148 126 122 110 85 69 44 24 15 9 NaeI GCC'GGC Cuts at: 2011 2086 3347 4067 4998 6129 6324 6547 8075 Size: 75 1261 720 931 1131 195 223 1528 Cuts at: 8075 2011 Size: 4776 Fragments arranged by size: 4776 1528 1261 1131 931 720 223 195 75 NarI GG'CG_CC Cuts at: 2544 2624 2838 2871 2970 3019 3065 4563 5010 Size: 80 214 33 99 49 46 1498 447 Cuts at: 5010 5698 6093 6117 6394 6543 6806 7947 2544 Size: 688 395 24 277 149 263 1141 5437 Fragments arranged by size: 5437 1498 1141 688 447 395 277 263 214 149 99 80 49 46 33 24 NcoI C'CATG_G Cuts at: 513 1065 2004 4396 6679 6796 513 Size: 552 939 2392 2283 117 4557 Fragments arranged by size: 4557 2392 2283 939 552 117 NdeI CA'TA_TG Cuts at: 387 387 Size: 10840 NgoMIV G'CCGG_C Cuts at: 2009 2084 3345 4065 4996 6127 6322 6545 8073 Size: 75 1261 720 931 1131 195 223 1528 Cuts at: 8073 2009 Size: 4776 Fragments arranged by size: 4776 1528 1261 1131 931 720 223 195 75 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10840 NotI GC'GGCC_GC Cuts at: 2036 5720 8287 2036 Size: 3684 2567 4589 Fragments arranged by size: 4589 3684 2567 NruI TCG'CGA Cuts at: 5071 5071 Size: 10840 NspI r_CATG'y Cuts at: 1132 1229 2097 3742 8198 9743 1132 Size: 97 868 1645 4456 1545 2229 Fragments arranged by size: 4456 2229 1645 1545 868 97 PasI CC'CwG_GG Cuts at: 1348 4243 4660 4970 5291 6853 7165 1348 Size: 2895 417 310 321 1562 312 5023 Fragments arranged by size: 5023 2895 1562 417 321 312 310 PciI A'CATG_T Cuts at: 9739 9739 Size: 10840 PflMI CCAn_nnn'nTGG Cuts at: 1108 3074 4900 5109 6592 6703 7819 7857 1108 Size: 1966 1826 209 1483 111 1116 38 4091 Fragments arranged by size: 4091 1966 1826 1483 1116 209 111 38 PfoI T'CCnGG_A Cuts at: 5415 5415 Size: 10840 PmeI GTTT'AAAC Cuts at: 8144 8144 Size: 10840 PmlI CAC'GTG Cuts at: 3748 3781 5737 7414 3748 Size: 33 1956 1677 7174 Fragments arranged by size: 7174 1956 1677 33 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2438 2470 6989 7021 7879 7911 8909 8941 10422 Size: 32 4519 32 858 32 998 32 1481 Cuts at: 10422 10454 2438 Size: 32 2824 Fragments arranged by size: 4519 2824 1481 998 858 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2478 2740 3599 5581 6030 6973 2478 Size: 262 859 1982 449 943 6345 Fragments arranged by size: 6345 1982 943 859 449 262 PshAI GACnn'nnGTC Cuts at: 1613 2961 7181 7625 1613 Size: 1348 4220 444 4828 Fragments arranged by size: 4828 4220 1348 444 PsiI TTA'TAA Cuts at: 8410 8410 Size: 10840 PspOMI G'GGCC_C Cuts at: 2026 3379 4663 4903 5588 5667 5865 6687 6905 Size: 1353 1284 240 685 79 198 822 218 Cuts at: 6905 8008 2026 Size: 1103 4858 Fragments arranged by size: 4858 1353 1284 1103 822 685 240 218 198 79 PspXI vC'TCGA_Gb Cuts at: 3487 5258 3487 Size: 1771 9069 PstI C_TGCA'G Cuts at: 830 2676 2765 3150 3480 4547 5730 6071 7671 Size: 1846 89 385 330 1067 1183 341 1600 Cuts at: 7671 7905 8192 830 Size: 234 287 3478 Fragments arranged by size: 3478 1846 1600 1183 1067 385 341 330 287 234 89 PvuI CG_AT'CG Cuts at: 2001 3122 2001 Size: 1121 9719 PvuII CAG'CTG Cuts at: 2994 3791 4128 4548 4852 6072 7372 7396 7957 Size: 797 337 420 304 1220 1300 24 561 Cuts at: 7957 2994 Size: 5877 Fragments arranged by size: 5877 1300 1220 797 561 420 337 304 24 SacII CC_GC'GG Cuts at: 2722 4671 2722 Size: 1949 8891 SalI G'TCGA_C Cuts at: 1936 8182 1936 Size: 6246 4594 SanDI GG'GwC_CC Cuts at: 2478 2740 2478 Size: 262 10578 SapI GCTCTTCn'nnn_ Cuts at: 2594 6764 2594 Size: 4170 6670 SbfI CC_TGCA'GG Cuts at: 3480 7905 8192 3480 Size: 4425 287 6128 Fragments arranged by size: 6128 4425 287 ScaI AGT'ACT Cuts at: 9088 10800 9088 Size: 1712 9128 SexAI A'CCwGG_T Cuts at: 2238 2319 2238 Size: 81 10759 SfcI C'TryA_G Cuts at: 826 1045 2653 2672 2761 3146 3277 3476 4177 Size: 219 1608 19 89 385 131 199 701 Cuts at: 4177 4543 5726 6067 7667 7901 8188 9323 10004 Size: 366 1183 341 1600 234 287 1135 681 Cuts at: 10004 10195 826 Size: 191 1471 Fragments arranged by size: 1608 1600 1471 1183 1135 701 681 385 366 341 287 234 219 199 191 131 89 19 SfiI GGCCn_nnn'nGGCC Cuts at: 7026 7026 Size: 10840 SfoI GGC'GCC Cuts at: 2545 2625 2839 2872 2971 3020 3066 4564 5011 Size: 80 214 33 99 49 46 1498 447 Cuts at: 5011 5699 6094 6118 6395 6544 6807 7948 2545 Size: 688 395 24 277 149 263 1141 5437 Fragments arranged by size: 5437 1498 1141 688 447 395 277 263 214 149 99 80 49 46 33 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10840 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10840 SmaI CCC'GGG Cuts at: 1872 2025 2249 2476 3134 3983 4349 4615 5666 Size: 153 224 227 658 849 366 266 1051 Cuts at: 5666 6251 7463 7537 8167 1872 Size: 585 1212 74 630 4545 Fragments arranged by size: 4545 1212 1051 849 658 630 585 366 266 227 224 153 74 SmlI C'TyrA_G Cuts at: 820 1017 1943 3487 5258 8944 9845 10107 820 Size: 197 926 1544 1771 3686 901 262 1553 Fragments arranged by size: 3686 1771 1553 1544 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4234 7297 8631 493 Size: 3741 3063 1334 2702 Fragments arranged by size: 3741 3063 2702 1334 SpeI A'CTAG_T Cuts at: 152 152 Size: 10840 SphI G_CATG'C Cuts at: 1132 3742 8198 1132 Size: 2610 4456 3774 Fragments arranged by size: 4456 3774 2610 SrfI GCCC'GGGC Cuts at: 3983 5666 3983 Size: 1683 9157 SspI AAT'ATT Cuts at: 5 52 8764 5 Size: 47 8712 2081 Fragments arranged by size: 8712 2081 47 StuI AGG'CCT Cuts at: 1517 6954 8027 1517 Size: 5437 1073 4330 Fragments arranged by size: 5437 4330 1073 StyI C'CwwG_G Cuts at: 513 1065 2004 4396 4558 4806 5265 5397 5706 Size: 552 939 2392 162 248 459 132 309 Cuts at: 5706 6679 6796 7602 8022 8279 513 Size: 973 117 806 420 257 3074 Fragments arranged by size: 3074 2392 973 939 806 552 459 420 309 257 248 162 132 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5167 7664 8867 8884 9037 9222 9667 Size: 1255 3587 2497 1203 17 153 185 445 Cuts at: 9667 325 Size: 1498 Fragments arranged by size: 3587 2497 1498 1255 1203 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4535 6234 9086 Size: 274 80 33 51 1443 2558 1699 2852 Cuts at: 9086 10798 96 Size: 1712 138 Fragments arranged by size: 2852 2558 1712 1699 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2180 2212 3804 3836 4094 4126 6186 6218 10800 Size: 32 1592 32 258 32 2060 32 4582 Cuts at: 10800 10832 2180 Size: 32 2188 Fragments arranged by size: 4582 2188 2060 1592 258 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3086 3107 3257 3521 3911 8102 1316 Size: 1770 21 150 264 390 4191 4054 Fragments arranged by size: 4191 4054 1770 390 264 150 21 XbaI T'CTAG_A Cuts at: 8176 8176 Size: 10840 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2327 3701 5234 7186 7714 2327 Size: 1374 1533 1952 528 5453 Fragments arranged by size: 5453 1952 1533 1374 528 XhoI C'TCGA_G Cuts at: 1943 3487 5258 1943 Size: 1544 1771 7525 Fragments arranged by size: 7525 1771 1544 XmaI C'CCGG_G Cuts at: 1870 2023 2247 2474 3132 3981 4347 4613 5664 Size: 153 224 227 658 849 366 266 1051 Cuts at: 5664 6249 7461 7535 8165 1870 Size: 585 1212 74 630 4545 Fragments arranged by size: 4545 1212 1051 849 658 630 585 366 266 227 224 153 74 XmnI GAAnn'nnTTC Cuts at: 2930 5558 8969 2930 Size: 2628 3411 4801 Fragments arranged by size: 4801 3411 2628 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5548 5966 6577 7432 Size: 53 83 186 936 4014 418 611 855 Cuts at: 7432 8648 276 Size: 1216 2468 Fragments arranged by size: 4014 2468 1216 936 855 611 418 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FspAI NsiI PacI PsrI RsrII SacI SwaI