Restriction map for
pFN21AB9482
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI Asp718I BamHI BbrPI
BciVI BfrI BglII BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DrdI DsaI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI HpaI
KpnI KspI MamI MfeI MluI
MroI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SbfI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1305 GTCGAC GT^[AC][GT]AC
AccII 198 CGCG CG^CG
AhdI 814 GACTACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 418 GTGCAC G^TGCAC
AlwNI 1288 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 949 CCTGAGG CC^T[ACGT]AGG
ApaI 889 GGGCCC GGGCC^C
ApaLI 418 GTGCAC G^TGCAC
AspEI 814 GACTACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 103 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 614 TGCGCA TGC^GCA
AvrII 9 CCTAGG C^CTAGG
BalI 719 TGGCCA TGG^CCA
BbvCI 1073 CCTCAGC CCTCAGC(-5/-2)
BclI 1517 TGATCA T^GATCA
BlnI 9 CCTAGG C^CTAGG
BlpI 1449 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1449 GCTGAGC GC^T[ACGT]AGC
BsaAI 120 TACGTG [CT]AC^GT[GA]
BsgI 1354 GTGCAG GTGCAG(16/14)
BspLU11I 818 ACATGT A^CATGT
BstUI 198 CGCG CG^CG
BstYI 1484 AGATCC [GA]^GATC[CT]
Bsu36I 949 CCTGAGG CC^T[ACGT]AGG
CelII 1449 GCTGAGC GC^T[ACGT]AGC
DraIII 381 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 814 GACTACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 949 CCTGAGG CC^T[ACGT]AGG
FbaI 1517 TGATCA T^GATCA
FspI 614 TGCGCA TGC^GCA
HgaI 1101 GACGC GACGC(5/10)
HindIII 220 AAGCTT A^AGCTT
MflI 1484 AGATCC [GA]^GATC[CT]
MluNI 719 TGGCCA TGG^CCA
MscI 719 TGGCCA TGG^CCA
MseI 92 TTAA T^TAA
MvnI 198 CGCG CG^CG
NspI 818 ACATGT [GA]CATG^[CT]
PaeR7I 1222 CTCGAG C^TCGAG
PflFI 103 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1253 CTGCAG CTGCA^G
SacI 1137 GAGCTC GAGCT^C
SalI 1305 GTCGAC G^TCGAC
SapI 847 GAAGAGC GCTCTTC(1/4)
SfcI 1253 CTGCAG C^T[GA][CT]AG
SfiI 399 GGCCTTCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I 92 TTAA T^TAA
Tsp509I 433 AATT ^AATT
Tth111I 103 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1287 CCAGAGCCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1222 CTCGAG C^TCGAG
XhoII 1484 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 249 GTAC GT^AC
429 GTAC
AlwI 1485 GATCC GGATC(4/5)
549 GGATC
Asp700I 513 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1422 GAAAAAGTTC
AspHI 418 GTGCAC G[AT]GC[AT]^C
1137 GAGCTC
BanII 889 GGGCCC G[GA]GC[CT]^C
1137 GAGCTC
BbeI 711 GGCGCC GGCGC^C
870 GGCGCC
BbsI 1098 GAAGAC GAAGAC(2/6)
1440 GAAGAC
BcgI 605 CGAGCGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1245 CGAGATGCCTGC
BcnI 300 CCGGG CC^[GC]GG
449 CCCGG
BfaI 10 CTAG C^TAG
17 CTAG
BpuAI 1098 GAAGAC GAAGAC(2/6)
1440 GAAGAC
BsiHKAI 418 GTGCAC G[AT]GC[AT]^C
1137 GAGCTC
BspMI 1415 ACCTGC ACCTGC(4/8)
1356 GCAGGT
BsrBI 142 GAGCGG CCGCTC(-3/-3)
1117 GAGCGG
EaeI 591 TGGCCG [CT]^GGCC[GA]
719 TGGCCA
EarI 1081 CTCTTC CTCTTC(1/4)
847 GAAGAG
HaeII 711 GGCGCC [GA]GCGC^[CT]
870 GGCGCC
KasI 711 GGCGCC G^GCGCC
870 GGCGCC
Ksp632I 1081 CTCTTC CTCTTC(1/4)
847 GAAGAG
MaeI 10 CTAG C^TAG
17 CTAG
NarI 711 GGCGCC GG^CGCC
870 GGCGCC
NciI 300 CCGGG CC^[GC]GG
449 CCCGG
PflMI 621 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
804 CCAAGAGGTGG
PpuMI 1281 GGGACCC [GA]G^G[AT]CC[CT]
1412 AGGACCT
RsaI 249 GTAC GT^AC
429 GTAC
SfoI 711 GGCGCC GGC^GCC
870 GGCGCC
TfiI 126 GAATC G^A[AT]TC
190 GAATC
Tsp45I 31 GTCAC ^GT[GC]AC
1360 GTGAC
Van91I 621 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
804 CCAAGAGGTGG
XmnI 513 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1422 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcyI AflIII AluI AvaI
AvaII BanI BbvI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SmlI StuI
StyI TaqI TseI TspRI TthHB8I