(Circular) (Six-base) MAPSORT of: pFN21AB9482.seq Check: 5690 from: 1 to: 6256 pFN21AB9482 6256 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3352 3352 Size: 6256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3311 3599 1937 Size: 1374 288 4594 Fragments arranged by size: 4594 1374 288 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2509 4319 5703 5971 1632 Size: 877 1810 1384 268 1917 Fragments arranged by size: 1917 1810 1384 877 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 2367 2823 3477 5155 6132 1203 Size: 1164 456 654 1678 977 1327 Fragments arranged by size: 1678 1327 1164 977 654 456 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 2824 4985 2824 Size: 2161 4095 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 3298 3999 5571 3298 Size: 701 1572 3983 Fragments arranged by size: 3983 1572 701 ApaI G_GGCC'C Cuts at: 2898 2898 Size: 6256 ApaLI G'TGCA_C Cuts at: 2423 4313 5469 2423 Size: 1890 1156 3210 Fragments arranged by size: 3210 1890 1156 ApoI r'AATT_y Cuts at: 1493 3565 3791 3943 6052 1493 Size: 2072 226 152 2109 1697 Fragments arranged by size: 2109 2072 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 2282 3227 3509 3581 1870 Size: 73 339 945 282 72 4545 Fragments arranged by size: 4545 945 339 282 73 72 AvrII C'CTAG_G Cuts at: 2014 2014 Size: 6256 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2716 2875 2989 3577 5032 Size: 325 217 627 929 159 114 588 1455 Cuts at: 5032 618 Size: 1842 Fragments arranged by size: 1842 1455 929 627 588 325 217 159 114 BanII G_rGCy'C Cuts at: 1391 2898 3146 3575 1391 Size: 1507 248 429 4072 Fragments arranged by size: 4072 1507 429 248 BbeI G_GCGC'C Cuts at: 2720 2879 2720 Size: 159 6097 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3110 3452 928 Size: 971 1211 342 3732 Fragments arranged by size: 3732 1211 971 342 BbvCI CC'TCA_GC Cuts at: 3079 3079 Size: 6256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2599 2633 3239 3273 4447 4481 1903 Size: 34 662 34 606 34 1174 34 3678 Fragments arranged by size: 3678 1174 662 606 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 3522 1548 Size: 1974 4282 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2411 2480 Size: 107 122 71 631 605 337 402 69 Cuts at: 2480 3165 4867 136 Size: 685 1702 1525 Fragments arranged by size: 1702 1525 685 631 605 402 337 122 107 71 69 BglII A'GATC_T Cuts at: 6251 6251 Size: 6256 BlpI GC'TnA_GC Cuts at: 3455 3673 3455 Size: 218 6038 Bme1580I G_kGCm'C Cuts at: 1790 2396 2427 2898 4317 5473 1790 Size: 606 31 471 1419 1156 2573 Fragments arranged by size: 2573 1419 1156 606 471 31 BmgBI CAC'GTC Cuts at: 1318 2370 3358 1318 Size: 1052 988 4216 Fragments arranged by size: 4216 1052 988 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2383 3987 4945 Size: 416 53 83 399 24 966 1604 958 Cuts at: 4945 442 Size: 1753 Fragments arranged by size: 1753 1604 966 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3094 3094 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 2654 2666 2821 4916 1239 Size: 926 489 12 155 2095 2579 Fragments arranged by size: 2579 2095 926 489 155 12 Bpu10I CC'TnA_GC Cuts at: 3079 3079 Size: 6256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2176 2252 2534 3130 3479 3533 4381 5246 5544 Size: 76 282 596 349 54 848 865 298 Cuts at: 5544 2176 Size: 2888 Fragments arranged by size: 2888 865 848 596 349 298 282 76 54 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4919 882 Size: 617 3420 2219 Fragments arranged by size: 3420 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2127 4047 493 Size: 711 923 1920 2702 Fragments arranged by size: 2702 1920 923 711 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2717 2876 3107 4063 Size: 53 83 186 936 1184 159 231 956 Cuts at: 4063 4445 5080 275 Size: 382 635 1451 Fragments arranged by size: 1451 1184 956 936 635 382 231 186 159 83 53 BsaWI w'CCGG_w Cuts at: 4689 5087 5361 5508 5919 4689 Size: 398 274 147 411 5026 Fragments arranged by size: 5026 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2639 2669 1178 Size: 30 165 30 1236 30 4765 Fragments arranged by size: 4765 1236 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 2198 2249 2492 2501 2504 2515 2591 Size: 1009 3 51 243 9 3 11 76 Cuts at: 2591 2594 2696 2903 2945 2954 2957 2972 2984 Size: 3 102 207 42 9 3 15 12 Cuts at: 2984 2987 2990 2993 3047 3065 3067 3187 1186 Size: 3 3 3 54 18 2 120 4255 Fragments arranged by size: 4255 1009 243 207 120 102 76 54 51 42 18 15 12 11 9 9 3 3 3 3 3 3 3 2 BseYI C'CCAG_C Cuts at: 3173 3318 5459 5955 3173 Size: 145 2141 496 3474 Fragments arranged by size: 3474 2141 496 145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3380 1163 Size: 573 1644 4039 Fragments arranged by size: 4039 1644 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3706 4467 5495 1540 Size: 275 186 1705 761 1028 2301 Fragments arranged by size: 2301 1705 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2427 3146 4317 4402 5473 2427 Size: 719 1171 85 1071 3210 Fragments arranged by size: 3210 1171 1071 719 85 BsmI GAATG_Cn' Cuts at: 3767 3860 3767 Size: 93 6163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2396 2427 2898 3146 3575 4317 4402 Size: 399 606 31 471 248 429 742 85 Cuts at: 4402 5473 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 606 471 429 399 248 85 31 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3352 3429 3611 844 Size: 2508 77 182 3489 Fragments arranged by size: 3489 2508 182 77 BsrBI CCG'CTC Cuts at: 1394 1823 2149 3124 4143 1394 Size: 429 326 975 1019 3507 Fragments arranged by size: 3507 1019 975 429 326 BsrDI GCAATG_nn' Cuts at: 65 1248 4751 4925 65 Size: 1183 3503 174 1396 Fragments arranged by size: 3503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4900 5087 1700 Size: 110 3090 187 2869 Fragments arranged by size: 3090 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6256 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6256 BstYI r'GATC_y Cuts at: 1972 3489 3586 3953 4338 4355 5807 5893 5905 Size: 1517 97 367 385 17 1452 86 12 Cuts at: 5905 6251 1972 Size: 346 1977 Fragments arranged by size: 1977 1517 1452 385 367 346 97 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2955 2955 Size: 6256 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4765 Fragments arranged by size: 4765 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2444 2802 487 Size: 683 56 1218 358 3941 Fragments arranged by size: 3941 1218 683 358 56 BtsI GCAGTG_nn' Cuts at: 2215 2469 3772 4566 4586 2215 Size: 254 1303 794 20 3885 Fragments arranged by size: 3885 1303 794 254 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3560 3560 Size: 6256 DraIII CAC_nnn'GTG Cuts at: 2391 2391 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2596 2724 3703 Size: 54 1425 50 275 18 766 128 979 Cuts at: 3703 4592 6221 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 979 889 766 275 128 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2847 3092 4186 1353 Size: 1494 245 1094 3423 Fragments arranged by size: 3423 1494 1094 245 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4843 5217 5363 387 Size: 1064 138 3254 374 146 1280 Fragments arranged by size: 3254 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3144 3144 Size: 6256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2509 2654 2666 2821 4319 4916 Size: 393 533 344 145 12 155 1498 597 Cuts at: 4916 5703 5971 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1498 787 597 533 393 344 268 155 145 12 EcoNI CCTnn'n_nnAGG Cuts at: 3551 3551 Size: 6256 EcoO109I rG'GnC_Cy Cuts at: 2018 2403 2894 3287 3418 2018 Size: 385 491 393 131 4856 Fragments arranged by size: 4856 491 393 385 131 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 FspI TGC'GCA Cuts at: 2621 4762 2621 Size: 2141 4115 HaeII r_GCGC'y Cuts at: 1678 2720 2879 5403 1678 Size: 1042 159 2524 2531 Fragments arranged by size: 2531 2524 1042 159 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2354 2386 2639 2671 2810 Size: 32 135 32 782 32 253 32 139 Cuts at: 2810 2842 4897 4929 4971 5003 1373 Size: 32 2055 32 42 32 2626 Fragments arranged by size: 2626 2055 782 253 139 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2116 2311 3312 3600 3846 669 Size: 988 281 178 195 1001 288 246 3079 Fragments arranged by size: 3079 1001 988 288 281 246 195 178 HindIII A'AGCT_T Cuts at: 748 2225 748 Size: 1477 4779 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KasI G'GCGC_C Cuts at: 2716 2875 2716 Size: 159 6097 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3018 5370 5554 1305 Size: 1713 2352 184 2007 Fragments arranged by size: 2352 2007 1713 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2726 10 Size: 54 1425 343 894 3540 Fragments arranged by size: 3540 1425 894 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4214 4573 4732 518 Size: 648 3048 359 159 2042 Fragments arranged by size: 3048 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2449 2542 2722 3351 3672 4349 5497 5742 Size: 843 93 180 629 321 677 1148 245 Cuts at: 5742 1606 Size: 2120 Fragments arranged by size: 2120 1148 843 677 629 321 245 180 93 NarI GG'CG_CC Cuts at: 2717 2876 2717 Size: 159 6097 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4765 Fragments arranged by size: 4765 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 2827 3614 5159 1132 Size: 97 1598 787 1545 2229 Fragments arranged by size: 2229 1598 1545 787 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6256 PciI A'CATG_T Cuts at: 2823 5155 2823 Size: 2332 3924 PflMI CCAn_nnn'nTGG Cuts at: 1108 2632 2815 1108 Size: 1524 183 4549 Fragments arranged by size: 4549 1524 183 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3287 3418 3287 Size: 131 6125 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PspOMI G'GGCC_C Cuts at: 2894 2894 Size: 6256 PspXI vC'TCGA_Gb Cuts at: 3227 3227 Size: 6256 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2357 2389 2357 Size: 32 6224 PstI C_TGCA'G Cuts at: 830 3262 3608 830 Size: 2432 346 3478 Fragments arranged by size: 3478 2432 346 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 SacI G_AGCT'C Cuts at: 3146 3146 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3310 3598 1936 Size: 1374 288 4594 Fragments arranged by size: 4594 1374 288 SanDI GG'GwC_CC Cuts at: 3287 3287 Size: 6256 SapI GCTCTTCn'nnn_ Cuts at: 2847 2847 Size: 6256 SbfI CC_TGCA'GG Cuts at: 3608 3608 Size: 6256 ScaI AGT'ACT Cuts at: 4504 6216 4504 Size: 1712 4544 SfcI C'TryA_G Cuts at: 826 1045 3258 3604 4739 5420 5611 826 Size: 219 2213 346 1135 681 191 1471 Fragments arranged by size: 2213 1471 1135 681 346 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2411 2411 Size: 6256 SfoI GGC'GCC Cuts at: 2718 2877 2718 Size: 159 6097 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 3583 1872 Size: 1711 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2155 2267 2549 3145 3227 3494 Size: 197 926 212 112 282 596 82 267 Cuts at: 3494 3548 4360 5261 5523 820 Size: 54 812 901 262 1553 Fragments arranged by size: 1553 926 901 812 596 282 267 262 212 197 112 82 54 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 3614 1132 Size: 2482 3774 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 2414 2774 3074 1517 Size: 897 360 300 4699 Fragments arranged by size: 4699 897 360 300 StyI C'CwwG_G Cuts at: 513 1065 2004 2014 2700 2727 2871 2898 3695 Size: 552 939 10 686 27 144 27 797 Cuts at: 3695 513 Size: 3074 Fragments arranged by size: 3074 939 797 686 552 144 27 27 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2402 3007 3127 3358 4283 4300 4453 Size: 1255 822 605 120 231 925 17 153 Cuts at: 4453 4638 5083 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 925 822 605 445 231 185 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4502 6214 96 Size: 274 80 33 51 1443 2525 1712 138 Fragments arranged by size: 2525 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6216 6248 6216 Size: 32 6224 Tth111I GACn'n_nGTC Cuts at: 1316 2111 1316 Size: 795 5461 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3299 3299 Size: 6256 XhoI C'TCGA_G Cuts at: 1943 3227 1943 Size: 1284 4972 XmaI C'CCGG_G Cuts at: 1870 3581 1870 Size: 1711 4545 XmnI GAAnn'nnTTC Cuts at: 2522 3431 4385 2522 Size: 909 954 4393 Fragments arranged by size: 4393 954 909 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PvuII RsrII SacII SexAI SrfI SwaI