Restriction map for
pFN21AB9479
(Linear) (Six-base) MAPSORT of: KIBB9479.seq Check: 4335 from: 1 to: 2193
KIBB9479.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1926 2193
Size: 1926 267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1162 2049 2113 2193
Size: 871 291 887 64 80
Fragments arranged by size:
887 871 291 80 64
AflIII A'CryG_T
Cuts at: 0 587 2193
Size: 587 1606
AleI CACnn'nnGTG
Cuts at: 0 328 457 2193
Size: 328 129 1736
Fragments arranged by size:
1736 328 129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1121 1153 2193
Size: 1121 32 1040
Fragments arranged by size:
1121 1040 32
AlwNI CAG_nnn'CTG
Cuts at: 0 19 100 360 387 658 894 999 1020
Size: 19 81 260 27 271 236 105 21
Cuts at: 1020 1233 1317 1326 1842 2193
Size: 213 84 9 516 351
Fragments arranged by size:
516 351 271 260 236 213 105 84
81 27 21 19 9
ApaI G_GGCC'C
Cuts at: 0 76 606 671 1008 1045 1612 2150 2193
Size: 76 530 65 337 37 567 538 43
Fragments arranged by size:
567 538 530 337 76 65 43 37
ApoI r'AATT_y
Cuts at: 0 1401 2193
Size: 1401 792
AvaI C'yCGr_G
Cuts at: 0 706 2193
Size: 706 1487
BanI G'GyrC_C
Cuts at: 0 244 519 1379 1749 2193
Size: 244 275 860 370 444
Fragments arranged by size:
860 444 370 275 244
BanII G_rGCy'C
Cuts at: 0 76 111 376 606 671 948 978 1008
Size: 76 35 265 230 65 277 30 30
Cuts at: 1008 1045 1612 2134 2150 2193
Size: 37 567 522 16 43
Fragments arranged by size:
567 522 277 265 230 76 65 43
37 35 30 30 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 799 2193
Size: 799 1394
BbvCI CC'TCA_GC
Cuts at: 0 509 1166 2193
Size: 509 657 1027
Fragments arranged by size:
1027 657 509
BglI GCCn_nnn'nGGC
Cuts at: 0 115 912 1372 2193
Size: 115 797 460 821
Fragments arranged by size:
821 797 460 115
BlpI GC'TnA_GC
Cuts at: 0 1289 1460 2193
Size: 1289 171 733
Fragments arranged by size:
1289 733 171
Bme1580I G_kGCm'C
Cuts at: 0 76 247 524 606 671 1008 1045 1612
Size: 76 171 277 82 65 337 37 567
Cuts at: 1612 1752 1785 2150 2162 2193
Size: 140 33 365 12 31
Fragments arranged by size:
567 365 337 277 171 140 82 76
65 37 33 31 12
BmgBI CAC'GTC
Cuts at: 0 459 2193
Size: 459 1734
BmrI ACTGGGnnnn_n'
Cuts at: 0 79 674 760 1344 1521 1819 2193
Size: 79 595 86 584 177 298 374
Fragments arranged by size:
595 584 374 298 177 86 79
BmtI G_CTAG'C
Cuts at: 0 1822 2193
Size: 1822 371
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1863 2193
Size: 1863 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 997 1000 1042 1180 1255 1348 1673
Size: 420 577 3 42 138 75 93 325
Cuts at: 1673 2008 2193
Size: 335 185
Fragments arranged by size:
577 420 335 325 185 138 93 75
42 3
Bpu10I CC'TnA_GC
Cuts at: 0 509 745 1166 1764 2193
Size: 509 236 421 598 429
Fragments arranged by size:
598 509 429 421 236
BsaI GGTCTCn'nnnn_
Cuts at: 0 302 313 1359 1442 2193
Size: 302 11 1046 83 751
Fragments arranged by size:
1046 751 302 83 11
BsaWI w'CCGG_w
Cuts at: 0 1451 2193
Size: 1451 742
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 281 311 2193
Size: 115 30 136 30 1882
Fragments arranged by size:
1882 136 115 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 241 436 622 934 937 1254 1588 1638
Size: 241 195 186 312 3 317 334 50
Cuts at: 1638 2017 2193
Size: 379 176
Fragments arranged by size:
379 334 317 312 241 195 186 176
50 3
BseYI C'CCAG_C
Cuts at: 0 29 157 544 570 787 860 932 1212
Size: 29 128 387 26 217 73 72 280
Cuts at: 1212 1269 1293 1885 2193
Size: 57 24 592 308
Fragments arranged by size:
592 387 308 280 217 128 73 72
57 29 26 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 2193
Size: 578 1615
BsiHKAI G_wGCw'C
Cuts at: 0 1466 2193
Size: 1466 727
BsmI GAATG_Cn'
Cuts at: 0 215 933 2193
Size: 215 718 1260
Fragments arranged by size:
1260 718 215
BsmBI CGTCTCn'nnnn_
Cuts at: 0 474 2193
Size: 474 1719
Bsp1286I G_dGCh'C
Cuts at: 0 76 111 247 376 524 606 671 948
Size: 76 35 136 129 148 82 65 277
Cuts at: 948 978 1008 1045 1466 1612 1752 1785 2134
Size: 30 30 37 421 146 140 33 349
Cuts at: 2134 2150 2162 2193
Size: 16 12 31
Fragments arranged by size:
421 349 277 148 146 140 136 129
82 76 65 37 35 33 31 30
30 16 12
BspHI T'CATG_A
Cuts at: 0 1950 2193
Size: 1950 243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 320 681 1926 2193
Size: 320 361 1245 267
Fragments arranged by size:
1245 361 320 267
BsrBI CCG'CTC
Cuts at: 0 687 2193
Size: 687 1506
BsrFI r'CCGG_y
Cuts at: 0 499 2193
Size: 499 1694
BsrGI T'GTAC_A
Cuts at: 0 1221 2193
Size: 1221 972
BstAPI GCAn_nnn'nTGC
Cuts at: 0 999 2193
Size: 999 1194
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1348 1651 2077 2193
Size: 1348 303 426 116
Fragments arranged by size:
1348 426 303 116
BstYI r'GATC_y
Cuts at: 0 1230 2193
Size: 1230 963
BtgI C'CryG_G
Cuts at: 0 164 326 515 671 1260 2193
Size: 164 162 189 156 589 933
Fragments arranged by size:
933 589 189 164 162 156
BtsI GCAGTG_nn'
Cuts at: 0 447 2193
Size: 447 1746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1657 1692 2193
Size: 1657 35 501
Fragments arranged by size:
1657 501 35
DraIII CAC_nnn'GTG
Cuts at: 0 1084 2193
Size: 1084 1109
DrdI GACnn_nn'nnGTC
Cuts at: 0 298 1351 2193
Size: 298 1053 842
Fragments arranged by size:
1053 842 298
EaeI y'GGCC_r
Cuts at: 0 824 1491 2193
Size: 824 667 702
Fragments arranged by size:
824 702 667
EarI CTCTTCn'nnn_
Cuts at: 0 190 640 875 2092 2193
Size: 190 450 235 1217 101
Fragments arranged by size:
1217 450 235 190 101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 871 997 1000 1042 1162 1180 1255
Size: 420 451 126 3 42 120 18 75
Cuts at: 1255 1348 1673 2008 2049 2113 2193
Size: 93 325 335 41 64 80
Fragments arranged by size:
451 420 335 325 126 120 93 80
75 64 42 41 18 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 284 2193
Size: 284 1909
EcoO109I rG'GnC_Cy
Cuts at: 0 117 357 602 603 627 693 762 1041
Size: 117 240 245 1 24 66 69 279
Cuts at: 1041 1108 1266 1323 1344 1608 1794 2146 2193
Size: 67 158 57 21 264 186 352 47
Fragments arranged by size:
352 279 264 245 240 186 158 117
69 67 66 57 47 24 21 1
EcoRI G'AATT_C
Cuts at: 0 1401 2193
Size: 1401 792
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1410 2193
Size: 1410 783
FseI GG_CCGG'CC
Cuts at: 0 503 2193
Size: 503 1690
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 2107 2139 2193
Size: 82 32 1993 32 54
Fragments arranged by size:
1993 82 54 32 32
MscI TGG'CCA
Cuts at: 0 826 2193
Size: 826 1367
MslI CAynn'nnrTG
Cuts at: 0 328 457 2193
Size: 328 129 1736
Fragments arranged by size:
1736 328 129
MspA1I CmG'CkG
Cuts at: 0 431 756 864 972 999 1158 1262 1893
Size: 431 325 108 108 27 159 104 631
Cuts at: 1893 2193
Size: 300
Fragments arranged by size:
631 431 325 300 159 108 108 104
27
NaeI GCC'GGC
Cuts at: 0 501 2193
Size: 501 1692
NcoI C'CATG_G
Cuts at: 0 164 515 671 2193
Size: 164 351 156 1522
Fragments arranged by size:
1522 351 164 156
NgoMIV G'CCGG_C
Cuts at: 0 499 2193
Size: 499 1694
NheI G'CTAG_C
Cuts at: 0 1818 2193
Size: 1818 375
NspI r_CATG'y
Cuts at: 0 591 2193
Size: 591 1602
PasI CC'CwG_GG
Cuts at: 0 1112 1348 1755 1880 2150 2193
Size: 1112 236 407 125 270 43
Fragments arranged by size:
1112 407 270 236 125 43
PciI A'CATG_T
Cuts at: 0 587 2193
Size: 587 1606
PflMI CCAn_nnn'nTGG
Cuts at: 0 1020 1305 2193
Size: 1020 285 888
Fragments arranged by size:
1020 888 285
PfoI T'CCnGG_A
Cuts at: 0 82 1967 2193
Size: 82 1885 226
Fragments arranged by size:
1885 226 82
PpuMI rG'GwC_Cy
Cuts at: 0 357 627 693 1266 1344 2193
Size: 357 270 66 573 78 849
Fragments arranged by size:
849 573 357 270 78 66
PshAI GACnn'nnGTC
Cuts at: 0 235 2193
Size: 235 1958
PspOMI G'GGCC_C
Cuts at: 0 72 602 667 1004 1041 1608 2146 2193
Size: 72 530 65 337 37 567 538 47
Fragments arranged by size:
567 538 530 337 72 65 47 37
PstI C_TGCA'G
Cuts at: 0 116 317 761 857 869 953 995 1322
Size: 116 201 444 96 12 84 42 327
Cuts at: 1322 2193
Size: 871
Fragments arranged by size:
871 444 327 201 116 96 84 42
12
PvuII CAG'CTG
Cuts at: 0 756 864 999 1158 1893 2193
Size: 756 108 135 159 735 300
Fragments arranged by size:
756 735 300 159 135 108
SacII CC_GC'GG
Cuts at: 0 1263 2193
Size: 1263 930
SanDI GG'GwC_CC
Cuts at: 0 1266 2193
Size: 1266 927
SapI GCTCTTCn'nnn_
Cuts at: 0 190 875 2193
Size: 190 685 1318
Fragments arranged by size:
1318 685 190
SbfI CC_TGCA'GG
Cuts at: 0 116 317 2193
Size: 116 201 1876
Fragments arranged by size:
1876 201 116
SfcI C'TryA_G
Cuts at: 0 112 313 757 853 865 949 991 1318
Size: 112 201 444 96 12 84 42 327
Cuts at: 1318 2193
Size: 875
Fragments arranged by size:
875 444 327 201 112 96 84 42
12
StuI AGG'CCT
Cuts at: 0 338 1375 2193
Size: 338 1037 818
Fragments arranged by size:
1037 818 338
StyI C'CwwG_G
Cuts at: 0 164 515 597 671 827 1564 1624 1898
Size: 164 351 82 74 156 737 60 274
Cuts at: 1898 2193
Size: 295
Fragments arranged by size:
737 351 295 274 164 156 82 74
60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 2193
Size: 21 2172
TatI w'GTAC_w
Cuts at: 0 1221 2193
Size: 1221 972
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 115 147 1372 1404 1417 1449 2193
Size: 115 32 1225 32 13 32 744
Fragments arranged by size:
1225 744 115 32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 0 781 1033 2193
Size: 781 252 1160
Fragments arranged by size:
1160 781 252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 522 678 1544 2193
Size: 522 156 866 649
Fragments arranged by size:
866 649 522 156
XmnI GAAnn'nnTTC
Cuts at: 0 884 2193
Size: 884 1309
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BbvCI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstXI BstYI
BtgI BtsI CspCI DraIII DrdI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI Hin4I MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacII
SanDI SapI SbfI SfcI StuI StyI TaqII TatI
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BglII BpuEI BsaAI
BsaBI BsaHI BsiEI BsiWI BspEI BsrDI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI EagI
EciI EcoICRI EcoRV FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NotI NruI NsiI PacI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacI SalI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XhoI XmaI ZraI