(Circular) (Six-base) MAPSORT of: pFN21AB9473.seq Check: 6011 from: 1 to: 7201 pFN21AB9473 7201 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5011 278 Size: 53 83 186 936 3475 2468 Fragments arranged by size: 3475 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2157 4544 1937 Size: 220 2387 4594 Fragments arranged by size: 4594 2387 220 Acc65I G'GTAC_C Cuts at: 4522 4522 Size: 7201 AclI AA'CG_TT Cuts at: 1563 5328 5701 1563 Size: 3765 373 3063 Fragments arranged by size: 3765 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2522 2823 3785 5264 6648 6916 1632 Size: 890 301 962 1479 1384 268 1917 Fragments arranged by size: 1917 1479 1384 962 890 301 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7004 AflIII A'CryG_T Cuts at: 1203 6100 7077 1203 Size: 4897 977 1327 Fragments arranged by size: 4897 1327 977 AgeI A'CCGG_T Cuts at: 6032 6032 Size: 7201 AhdI GACnn_n'nnGTC Cuts at: 5930 5930 Size: 7201 AleI CACnn'nnGTG Cuts at: 1166 4271 1166 Size: 3105 4096 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7201 AlwNI CAG_nnn'CTG Cuts at: 2434 4320 4944 6516 2434 Size: 1886 624 1572 3119 Fragments arranged by size: 3119 1886 1572 624 ApaLI G'TGCA_C Cuts at: 5258 6414 5258 Size: 1156 6045 ApoI r'AATT_y Cuts at: 1493 2699 3330 3993 4510 4736 4888 6997 1493 Size: 1206 631 663 517 226 152 2109 1697 Fragments arranged by size: 2109 1697 1206 663 631 517 226 152 AseI AT'TA_AT Cuts at: 160 1032 5755 160 Size: 872 4723 1606 Fragments arranged by size: 4723 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7201 AvaI C'yCGr_G Cuts at: 1870 1943 2069 4344 4526 1870 Size: 73 126 2275 182 4545 Fragments arranged by size: 4545 2275 182 126 73 BamHI G'GATC_C Cuts at: 4531 4898 4531 Size: 367 6834 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 2104 2138 2461 4522 Size: 325 217 627 286 31 34 323 2061 Cuts at: 4522 5977 618 Size: 1455 1842 Fragments arranged by size: 2061 1842 1455 627 325 323 286 217 34 31 BanII G_rGCy'C Cuts at: 1391 3580 4520 1391 Size: 2189 940 4072 Fragments arranged by size: 4072 2189 940 BbeI G_GCGC'C Cuts at: 2142 2142 Size: 7201 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2938 4489 928 Size: 971 1039 1551 3640 Fragments arranged by size: 3640 1551 1039 971 BbvCI CC'TCA_GC Cuts at: 2177 3932 2177 Size: 1755 5446 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5392 5426 1903 Size: 34 50 34 3371 34 3678 Fragments arranged by size: 3678 3371 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4043 5093 6303 4043 Size: 1050 1210 4941 Fragments arranged by size: 4941 1210 1050 BclI T'GATC_A Cuts at: 1548 1548 Size: 7201 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5812 136 Size: 107 122 71 631 605 4140 1525 Fragments arranged by size: 4140 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7196 7196 Size: 7201 BlpI GC'TnA_GC Cuts at: 4618 4618 Size: 7201 Bme1580I G_kGCm'C Cuts at: 1790 2076 2672 5262 6418 1790 Size: 286 596 2590 1156 2573 Fragments arranged by size: 2590 2573 1156 596 286 BmgBI CAC'GTC Cuts at: 1318 2593 1318 Size: 1275 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4235 4932 5890 Size: 416 53 83 399 24 2818 697 958 Cuts at: 5890 442 Size: 1753 Fragments arranged by size: 2818 1753 958 697 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3929 1056 Size: 2873 4328 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3148 3148 Size: 7201 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3118 5861 1239 Size: 1879 2743 2579 Fragments arranged by size: 2743 2579 1879 Bpu10I CC'TnA_GC Cuts at: 2177 3932 2177 Size: 1755 5446 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5326 6191 6489 5326 Size: 865 298 6038 Fragments arranged by size: 6038 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3638 4476 5864 882 Size: 617 2139 838 1388 2219 Fragments arranged by size: 2219 2139 1388 838 617 BsaAI yAC'GTr Cuts at: 493 1204 3517 4992 493 Size: 711 2313 1475 2702 Fragments arranged by size: 2702 2313 1475 711 BsaBI GATnn'nnATC Cuts at: 2148 7195 2148 Size: 5047 2154 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2063 2139 3474 5008 Size: 53 83 186 936 530 76 1335 1534 Cuts at: 5008 5390 6025 275 Size: 382 635 1451 Fragments arranged by size: 1534 1451 1335 936 635 530 382 186 83 76 53 BsaWI w'CCGG_w Cuts at: 2517 3258 3303 5634 6032 6306 6453 6864 2517 Size: 741 45 2331 398 274 147 411 2854 Fragments arranged by size: 2854 2331 741 411 398 274 147 45 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6976 Fragments arranged by size: 6976 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2371 3148 3221 1186 Size: 1185 777 73 5166 Fragments arranged by size: 5166 1185 777 73 BseYI C'CCAG_C Cuts at: 4159 4320 6404 6900 4159 Size: 161 2084 496 4460 Fragments arranged by size: 4460 2084 496 161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6628 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4338 4651 5412 6440 1540 Size: 275 186 2337 313 761 1028 2301 Fragments arranged by size: 2337 2301 1028 761 313 275 186 BsiHKAI G_wGCw'C Cuts at: 2476 3463 3678 5262 5347 6418 2476 Size: 987 215 1584 85 1071 3259 Fragments arranged by size: 3259 1584 1071 987 215 85 BsmI GAATG_Cn' Cuts at: 4365 4712 4805 4365 Size: 347 93 6761 Fragments arranged by size: 6761 347 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2408 2408 Size: 7201 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2476 2672 3463 3580 3678 4520 Size: 399 286 400 196 791 117 98 842 Cuts at: 4520 5262 5347 6418 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 842 791 742 400 399 286 196 117 98 85 BspEI T'CCGG_A Cuts at: 2517 3258 6864 2517 Size: 741 3606 2854 Fragments arranged by size: 3606 2854 741 BspHI T'CATG_A Cuts at: 4209 5090 6820 4209 Size: 881 1730 4590 Fragments arranged by size: 4590 1730 881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2314 4556 844 Size: 1470 2242 3489 Fragments arranged by size: 3489 2242 1470 BsrBI CCG'CTC Cuts at: 1394 1823 4249 5088 1394 Size: 429 2426 839 3507 Fragments arranged by size: 3507 2426 839 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3078 3354 3651 5696 5870 65 Size: 1183 1830 276 297 2045 174 1396 Fragments arranged by size: 2045 1830 1396 1183 297 276 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5845 6032 1700 Size: 110 4035 187 2869 Fragments arranged by size: 4035 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3738 96 Size: 3642 3559 BssHII G'CGCG_C Cuts at: 1925 2037 1925 Size: 112 7089 BssSI C'ACGA_G Cuts at: 5261 6273 5261 Size: 1012 6189 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7201 BstBI TT'CG_AA Cuts at: 6005 6005 Size: 7201 BstEII G'GTnAC_C Cuts at: 1184 3844 1184 Size: 2660 4541 BstXI CCAn_nnnn'nTGG Cuts at: 2593 2593 Size: 7201 BstYI r'GATC_y Cuts at: 1972 2822 4488 4531 4898 5283 5300 6752 6838 Size: 850 1666 43 367 385 17 1452 86 Cuts at: 6838 6850 7196 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1666 1452 850 385 367 346 86 43 17 12 BtgI C'CryG_G Cuts at: 513 1065 2457 3201 4011 513 Size: 552 1392 744 810 3703 Fragments arranged by size: 3703 1392 810 744 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6462 Fragments arranged by size: 6462 683 56 BtsI GCAGTG_nn' Cuts at: 4046 4717 5511 5531 4046 Size: 671 794 20 5716 Fragments arranged by size: 5716 794 671 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7201 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7166 DraI TTT'AAA Cuts at: 4505 4505 Size: 7201 DraIII CAC_nnn'GTG Cuts at: 2695 2695 Size: 7201 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6208 809 Size: 507 4892 1802 Fragments arranged by size: 4892 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4263 4335 4648 Size: 54 1425 50 275 18 2433 72 313 Cuts at: 4648 5537 7166 8 Size: 889 1629 43 Fragments arranged by size: 2433 1629 1425 889 313 275 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4335 4648 1537 Size: 275 2523 313 4090 Fragments arranged by size: 4090 2523 313 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2342 3539 3641 4256 5131 1353 Size: 989 1197 102 615 875 3423 Fragments arranged by size: 3423 1197 989 875 615 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5788 6162 6308 387 Size: 1064 138 4199 374 146 1280 Fragments arranged by size: 4199 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 2823 3118 3785 5264 5861 6648 Size: 393 890 301 295 667 1479 597 787 Cuts at: 6648 6916 1239 Size: 268 1524 Fragments arranged by size: 1524 1479 890 787 667 597 393 301 295 268 EcoO109I rG'GnC_Cy Cuts at: 2420 4234 2420 Size: 1814 5387 EcoRI G'AATT_C Cuts at: 4510 4888 4510 Size: 378 6823 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3613 3613 Size: 7201 FspI TGC'GCA Cuts at: 5707 5707 Size: 7201 HaeII r_GCGC'y Cuts at: 1678 2142 3807 6348 1678 Size: 464 1665 2541 2531 Fragments arranged by size: 2541 2531 1665 464 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 2438 2470 2582 Size: 32 135 32 728 32 106 32 112 Cuts at: 2582 2614 2845 2877 4473 4505 5842 5874 5916 Size: 32 231 32 1596 32 1337 32 42 Cuts at: 5916 5948 1373 Size: 32 2626 Fragments arranged by size: 2626 1596 1337 728 231 135 112 106 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4099 4449 4545 4791 669 Size: 988 281 2161 350 96 246 3079 Fragments arranged by size: 3079 2161 988 350 281 246 96 HindIII A'AGCT_T Cuts at: 748 3031 748 Size: 2283 4918 HpaI GTT'AAC Cuts at: 4099 4449 4791 4099 Size: 350 342 6509 Fragments arranged by size: 6509 350 342 KasI G'GCGC_C Cuts at: 2138 2138 Size: 7201 KpnI G_GTAC'C Cuts at: 4526 4526 Size: 7201 MfeI C'AATT_G Cuts at: 4800 4800 Size: 7201 MluI A'CGCG_T Cuts at: 7077 7077 Size: 7201 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3735 6315 6499 1305 Size: 2430 2580 184 2007 Fragments arranged by size: 2580 2430 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4265 10 Size: 54 1425 343 2433 2946 Fragments arranged by size: 2946 2433 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2612 3359 3740 4208 4271 5159 5518 Size: 648 1446 747 381 468 63 888 359 Cuts at: 5518 5677 518 Size: 159 2042 Fragments arranged by size: 2042 1446 888 747 648 468 381 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2163 3388 4271 4320 4617 5294 6442 6687 Size: 557 1225 883 49 297 677 1148 245 Cuts at: 6687 1606 Size: 2120 Fragments arranged by size: 2120 1225 1148 883 677 557 297 245 49 NarI GG'CG_CC Cuts at: 2139 2139 Size: 7201 NcoI C'CATG_G Cuts at: 513 1065 2457 4011 513 Size: 552 1392 1554 3703 Fragments arranged by size: 3703 1554 1392 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7201 NheI G'CTAG_C Cuts at: 1052 3925 1052 Size: 2873 4328 NotI GC'GGCC_GC Cuts at: 4648 4648 Size: 7201 NspI r_CATG'y Cuts at: 1132 1229 3763 4159 4559 6104 1132 Size: 97 2534 396 400 1545 2229 Fragments arranged by size: 2534 2229 1545 400 396 97 PasI CC'CwG_GG Cuts at: 1348 2467 4137 1348 Size: 1119 1670 4412 Fragments arranged by size: 4412 1670 1119 PciI A'CATG_T Cuts at: 6100 6100 Size: 7201 PflMI CCAn_nnn'nTGG Cuts at: 1108 2695 3916 1108 Size: 1587 1221 4393 Fragments arranged by size: 4393 1587 1221 PfoI T'CCnGG_A Cuts at: 2835 3139 3293 2835 Size: 304 154 6743 Fragments arranged by size: 6743 304 154 PmeI GTTT'AAAC Cuts at: 4505 4505 Size: 7201 PmlI CAC'GTG Cuts at: 3517 3517 Size: 7201 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2180 2212 2243 2275 5270 5302 6783 6815 2180 Size: 32 31 32 2995 32 1481 32 2566 Fragments arranged by size: 2995 2566 1481 32 32 32 32 31 PpuMI rG'GwC_Cy Cuts at: 2420 4234 2420 Size: 1814 5387 PshAI GACnn'nnGTC Cuts at: 1613 2894 1613 Size: 1281 5920 PsiI TTA'TAA Cuts at: 4771 4771 Size: 7201 PstI C_TGCA'G Cuts at: 830 4553 830 Size: 3723 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7201 PvuII CAG'CTG Cuts at: 3388 4320 3388 Size: 932 6269 SalI G'TCGA_C Cuts at: 1936 4543 1936 Size: 2607 4594 SanDI GG'GwC_CC Cuts at: 2420 2420 Size: 7201 SapI GCTCTTCn'nnn_ Cuts at: 2342 4256 2342 Size: 1914 5287 SbfI CC_TGCA'GG Cuts at: 4553 4553 Size: 7201 ScaI AGT'ACT Cuts at: 5449 7161 5449 Size: 1712 5489 SexAI A'CCwGG_T Cuts at: 2223 2223 Size: 7201 SfcI C'TryA_G Cuts at: 826 1045 2158 2747 2864 4549 5684 6365 6556 Size: 219 1113 589 117 1685 1135 681 191 Cuts at: 6556 826 Size: 1471 Fragments arranged by size: 1685 1471 1135 1113 681 589 219 191 117 SfoI GGC'GCC Cuts at: 2140 2140 Size: 7201 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7201 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7201 SmaI CCC'GGG Cuts at: 1872 2071 4346 4528 1872 Size: 199 2275 182 4545 Fragments arranged by size: 4545 2275 199 182 SmlI C'TyrA_G Cuts at: 820 1017 1943 5305 6206 6468 820 Size: 197 926 3362 901 262 1553 Fragments arranged by size: 3362 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4992 493 Size: 4499 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7201 SphI G_CATG'C Cuts at: 1132 3763 4559 1132 Size: 2631 796 3774 Fragments arranged by size: 3774 2631 796 SrfI GCCC'GGGC Cuts at: 2071 2071 Size: 7201 SspI AAT'ATT Cuts at: 5 52 5125 5 Size: 47 5073 2081 Fragments arranged by size: 5073 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7201 StyI C'CwwG_G Cuts at: 513 1065 2341 2457 3468 3588 3853 4011 4640 Size: 552 1276 116 1011 120 265 158 629 Cuts at: 4640 513 Size: 3074 Fragments arranged by size: 3074 1276 1011 629 552 265 158 120 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2459 3801 3953 5228 5245 5398 5583 Size: 1255 879 1342 152 1275 17 153 185 Cuts at: 5583 6028 325 Size: 445 1498 Fragments arranged by size: 1498 1342 1275 1255 879 445 185 153 152 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2478 3738 5447 Size: 274 80 33 51 1443 501 1260 1709 Cuts at: 5447 7159 96 Size: 1712 138 Fragments arranged by size: 1712 1709 1443 1260 501 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2377 2409 3048 3080 7161 7193 2377 Size: 32 639 32 4081 32 2385 Fragments arranged by size: 4081 2385 639 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7201 XbaI T'CTAG_A Cuts at: 4537 4537 Size: 7201 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2464 2464 Size: 7201 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7201 XmaI C'CCGG_G Cuts at: 1870 2069 4344 4526 1870 Size: 199 2275 182 4545 Fragments arranged by size: 4545 2275 199 182 XmnI GAAnn'nnTTC Cuts at: 2507 5330 2507 Size: 2823 4378 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5009 276 Size: 53 83 186 936 3475 2468 Fragments arranged by size: 3475 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SfiI SwaI