(Circular) (Six-base) MAPSORT of: pFN21AB9468.seq Check: 3186 from: 1 to: 11137 pFN21AB9468 11137 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2012 8947 278 Size: 53 83 186 936 476 6935 2468 Fragments arranged by size: 6935 2468 936 476 186 83 53 AccI GT'mk_AC Cuts at: 1937 7019 7269 8480 1937 Size: 5082 250 1211 4594 Fragments arranged by size: 5082 4594 1211 250 Acc65I G'GTAC_C Cuts at: 8458 8458 Size: 11137 AclI AA'CG_TT Cuts at: 1563 4112 6117 9264 9637 1563 Size: 2549 2005 3147 373 3063 Fragments arranged by size: 3147 3063 2549 2005 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3126 3188 3631 4118 4177 4890 5054 9200 Size: 1494 62 443 487 59 713 164 4146 Cuts at: 9200 10584 10852 1632 Size: 1384 268 1917 Fragments arranged by size: 4146 1917 1494 1384 713 487 443 268 164 62 59 AfeI AGC'GCT Cuts at: 6861 6861 Size: 11137 AflII C'TTAA_G Cuts at: 820 1017 2324 3731 820 Size: 197 1307 1407 8226 Fragments arranged by size: 8226 1407 1307 197 AflIII A'CryG_T Cuts at: 1203 6169 10036 11013 1203 Size: 4966 3867 977 1327 Fragments arranged by size: 4966 3867 1327 977 AgeI A'CCGG_T Cuts at: 9968 9968 Size: 11137 AhdI GACnn_n'nnGTC Cuts at: 9866 9866 Size: 11137 AleI CACnn'nnGTG Cuts at: 1166 2338 5156 7091 1166 Size: 1172 2818 1935 5212 Fragments arranged by size: 5212 2818 1935 1172 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6548 6854 1184 Size: 5364 306 5467 Fragments arranged by size: 5467 5364 306 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4609 4641 4609 Size: 32 11105 AlwNI CAG_nnn'CTG Cuts at: 2026 3440 4913 6335 6577 8212 8398 8880 10452 Size: 1414 1473 1422 242 1635 186 482 1572 Cuts at: 10452 2026 Size: 2711 Fragments arranged by size: 2711 1635 1572 1473 1422 1414 482 242 186 ApaLI G'TGCA_C Cuts at: 5270 9194 10350 5270 Size: 3924 1156 6057 Fragments arranged by size: 6057 3924 1156 ApoI r'AATT_y Cuts at: 1493 2842 3489 4021 4388 5210 6233 6338 6545 Size: 1349 647 532 367 822 1023 105 207 Cuts at: 6545 7042 8111 8446 8672 8824 10933 1493 Size: 497 1069 335 226 152 2109 1697 Fragments arranged by size: 2109 1697 1349 1069 1023 822 647 532 497 367 335 226 207 152 105 AseI AT'TA_AT Cuts at: 160 1032 2463 3752 5765 9691 160 Size: 872 1431 1289 2013 3926 1606 Fragments arranged by size: 3926 2013 1606 1431 1289 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11137 AvaI C'yCGr_G Cuts at: 1870 1943 2623 7461 8462 1870 Size: 73 680 4838 1001 4545 Fragments arranged by size: 4838 4545 1001 680 73 BamHI G'GATC_C Cuts at: 4290 5236 8467 8834 4290 Size: 946 3231 367 6593 Fragments arranged by size: 6593 3231 946 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4520 5683 6186 8458 9913 Size: 325 217 627 2733 1163 503 2272 1455 Cuts at: 9913 618 Size: 1842 Fragments arranged by size: 2733 2272 1842 1455 1163 627 503 325 217 BanII G_rGCy'C Cuts at: 1391 4329 5151 7584 7642 8456 1391 Size: 2938 822 2433 58 814 4072 Fragments arranged by size: 4072 2938 2433 822 814 58 BbeI G_GCGC'C Cuts at: 6190 6190 Size: 11137 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4183 4470 4724 5771 6573 6914 928 Size: 971 2284 287 254 1047 802 341 5151 Fragments arranged by size: 5151 2284 1047 971 802 341 287 254 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6296 6330 8142 8176 9328 9362 1903 Size: 34 4359 34 1812 34 1152 34 3678 Fragments arranged by size: 4359 3678 1812 1152 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 9029 10239 9029 Size: 1210 9927 BclI T'GATC_A Cuts at: 1548 2892 3781 6813 1548 Size: 1344 889 3032 5872 Fragments arranged by size: 5872 3032 1344 889 BfrBI ATG'CAT Cuts at: 8148 8148 Size: 11137 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9748 136 Size: 107 122 71 631 605 8076 1525 Fragments arranged by size: 8076 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2557 3361 5578 11132 2557 Size: 804 2217 5554 2562 Fragments arranged by size: 5554 2562 2217 804 BlpI GC'TnA_GC Cuts at: 2632 3458 8554 2632 Size: 826 5096 5215 Fragments arranged by size: 5215 5096 826 Bme1580I G_kGCm'C Cuts at: 1790 4312 5274 5531 5933 9198 10354 1790 Size: 2522 962 257 402 3265 1156 2573 Fragments arranged by size: 3265 2573 2522 1156 962 402 257 BmgBI CAC'GTC Cuts at: 1318 6172 1318 Size: 4854 6283 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4289 6230 7070 Size: 416 53 83 399 24 2872 1941 840 Cuts at: 7070 8868 9826 442 Size: 1798 958 1753 Fragments arranged by size: 2872 1941 1798 1753 958 840 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2225 3198 1056 Size: 1169 973 8995 Fragments arranged by size: 8995 1169 973 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5645 7186 7273 7603 8438 5645 Size: 1541 87 330 835 8344 Fragments arranged by size: 8344 1541 835 330 87 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2366 2532 4740 4814 5519 5790 6389 6677 Size: 1127 166 2208 74 705 271 599 288 Cuts at: 6677 7092 7199 7243 7391 7640 7682 8408 9797 Size: 415 107 44 148 249 42 726 1389 Cuts at: 9797 1239 Size: 2579 Fragments arranged by size: 2579 2208 1389 1127 726 705 599 415 288 271 249 166 148 107 74 44 42 Bpu10I CC'TnA_GC Cuts at: 2014 2014 Size: 11137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4662 9262 10127 10425 4662 Size: 4600 865 298 5374 Fragments arranged by size: 5374 4600 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6653 7261 9800 882 Size: 617 5154 608 2539 2219 Fragments arranged by size: 5154 2539 2219 617 608 BsaAI yAC'GTr Cuts at: 493 1204 8928 493 Size: 711 7724 2702 Fragments arranged by size: 7724 2702 711 BsaBI GATnn'nnATC Cuts at: 2755 6398 11131 2755 Size: 3643 4733 2761 Fragments arranged by size: 4733 3643 2761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 6187 8944 9326 Size: 53 83 186 936 476 4178 2757 382 Cuts at: 9326 9961 275 Size: 635 1451 Fragments arranged by size: 4178 2757 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5950 9570 9968 10242 10389 10800 5950 Size: 3620 398 274 147 411 6287 Fragments arranged by size: 6287 3620 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2025 2055 5988 6018 1178 Size: 30 165 30 622 30 3933 30 6297 Fragments arranged by size: 6297 3933 622 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4094 4097 4910 5858 6239 7136 8221 1186 Size: 2908 3 813 948 381 897 1085 4102 Fragments arranged by size: 4102 2908 1085 948 897 813 381 3 BseYI C'CCAG_C Cuts at: 2019 3921 7197 7205 7641 8025 10340 10836 2019 Size: 1902 3276 8 436 384 2315 496 2320 Fragments arranged by size: 3276 2320 2315 1902 496 436 384 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3887 4466 6246 6702 6980 7071 1163 Size: 573 2151 579 1780 456 278 91 5229 Fragments arranged by size: 5229 2151 1780 579 573 456 278 91 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8587 9348 10376 1540 Size: 275 186 6586 761 1028 2301 Fragments arranged by size: 6586 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2652 2925 4137 4329 5274 5437 6183 7584 9198 Size: 273 1212 192 945 163 746 1401 1614 Cuts at: 9198 9283 10354 2652 Size: 85 1071 3435 Fragments arranged by size: 3435 1614 1401 1212 1071 945 746 273 192 163 85 BsmI GAATG_Cn' Cuts at: 5304 7347 8648 8741 5304 Size: 2043 1301 93 7700 Fragments arranged by size: 7700 2043 1301 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3076 4144 7424 3076 Size: 1068 3280 6789 Fragments arranged by size: 6789 3280 1068 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2652 2925 4137 4312 4329 5151 5274 Size: 399 862 273 1212 175 17 822 123 Cuts at: 5274 5437 5531 5933 6183 7584 7642 8456 9198 Size: 163 94 402 250 1401 58 814 742 Cuts at: 9198 9283 10354 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1401 1212 1071 862 822 814 742 402 399 273 250 175 163 123 94 85 58 17 BspEI T'CCGG_A Cuts at: 5950 10800 5950 Size: 4850 6287 BspHI T'CATG_A Cuts at: 3519 9026 10756 3519 Size: 5507 1730 3900 Fragments arranged by size: 5507 3900 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 7281 8492 844 Size: 6437 1211 3489 Fragments arranged by size: 6437 3489 1211 BsrBI CCG'CTC Cuts at: 1394 1823 9024 1394 Size: 429 7201 3507 Fragments arranged by size: 7201 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2397 4707 7830 9632 9806 65 Size: 1183 1149 2310 3123 1802 174 1396 Fragments arranged by size: 3123 2310 1802 1396 1183 1149 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7868 9781 9968 1700 Size: 110 6058 1913 187 2869 Fragments arranged by size: 6058 2869 1913 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 11137 BssHII G'CGCG_C Cuts at: 1925 6736 6738 7143 1925 Size: 4811 2 405 5919 Fragments arranged by size: 5919 4811 405 2 BssSI C'ACGA_G Cuts at: 2420 9197 10209 2420 Size: 6777 1012 3348 Fragments arranged by size: 6777 3348 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3052 5557 794 Size: 2258 2505 6374 Fragments arranged by size: 6374 2505 2258 BstBI TT'CG_AA Cuts at: 9941 9941 Size: 11137 BstEII G'GTnAC_C Cuts at: 1184 7165 1184 Size: 5981 5156 BstXI CCAn_nnnn'nTGG Cuts at: 7252 7252 Size: 11137 BstYI r'GATC_y Cuts at: 1972 2557 3361 4030 4290 5136 5236 5578 7063 Size: 585 804 669 260 846 100 342 1485 Cuts at: 7063 8467 8834 9219 9236 10688 10774 10786 11132 Size: 1404 367 385 17 1452 86 12 346 Cuts at: 11132 1972 Size: 1977 Fragments arranged by size: 1977 1485 1452 1404 846 804 669 585 385 367 346 342 260 100 86 17 12 BstZ17I GTA'TAC Cuts at: 7020 7020 Size: 11137 Bsu36I CC'TnA_GG Cuts at: 2103 8238 2103 Size: 6135 5002 BtgI C'CryG_G Cuts at: 513 1065 2823 4350 5175 6696 7710 8169 513 Size: 552 1758 1527 825 1521 1014 459 3481 Fragments arranged by size: 3481 1758 1527 1521 1014 825 552 459 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2704 8134 487 Size: 683 56 1478 5430 3490 Fragments arranged by size: 5430 3490 1478 683 56 BtsI GCAGTG_nn' Cuts at: 2545 4248 4660 5455 6462 7086 8653 9447 9467 Size: 1703 412 795 1007 624 1567 794 20 Cuts at: 9467 2545 Size: 4215 Fragments arranged by size: 4215 1703 1567 1007 795 794 624 412 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11137 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3780 3815 529 Size: 35 3216 35 7851 Fragments arranged by size: 7851 3216 35 35 DraI TTT'AAA Cuts at: 4158 5229 5458 6544 6633 8441 4158 Size: 1071 229 1086 89 1808 6854 Fragments arranged by size: 6854 1808 1086 1071 229 89 DraIII CAC_nnn'GTG Cuts at: 5432 6224 7879 5432 Size: 792 1655 8690 Fragments arranged by size: 8690 1655 792 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 10144 809 Size: 507 8828 1802 Fragments arranged by size: 8828 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 3495 4347 Size: 54 1425 50 275 18 282 1383 852 Cuts at: 4347 6951 7188 7870 8166 8584 9473 11102 8 Size: 2604 237 682 296 418 889 1629 43 Fragments arranged by size: 2604 1629 1425 1383 889 852 682 418 296 282 275 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8584 1537 Size: 275 6772 4090 Fragments arranged by size: 6772 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2157 3045 3204 3756 4317 5237 6117 6639 Size: 804 888 159 552 561 920 880 522 Cuts at: 6639 7572 8100 9067 1353 Size: 933 528 967 3423 Fragments arranged by size: 3423 967 933 920 888 880 804 561 552 528 522 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5870 8189 9724 10098 10244 387 Size: 1064 138 4281 2319 1535 374 146 1280 Fragments arranged by size: 4281 2319 1535 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4327 7582 4327 Size: 3255 7882 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2366 2532 3126 3188 3631 4118 4177 Size: 393 734 166 594 62 443 487 59 Cuts at: 4177 4740 4814 4890 5054 5519 5790 6389 6677 Size: 563 74 76 164 465 271 599 288 Cuts at: 6677 7092 7199 7243 7391 7640 7682 8408 9200 Size: 415 107 44 148 249 42 726 792 Cuts at: 9200 9797 10584 10852 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 792 787 734 726 599 597 594 563 487 465 443 415 393 288 271 268 249 166 164 148 107 76 74 62 59 44 42 EcoO109I rG'GnC_Cy Cuts at: 4834 7624 7743 4834 Size: 2790 119 8228 Fragments arranged by size: 8228 2790 119 EcoRI G'AATT_C Cuts at: 2842 3489 8446 8824 2842 Size: 647 4957 378 5155 Fragments arranged by size: 5155 4957 647 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2186 3154 3167 3193 3950 7987 2186 Size: 968 13 26 757 4037 5336 Fragments arranged by size: 5336 4037 968 757 26 13 FspI TGC'GCA Cuts at: 9643 9643 Size: 11137 HaeII r_GCGC'y Cuts at: 1678 2687 6190 6863 7187 10284 1678 Size: 1009 3503 673 324 3097 2531 Fragments arranged by size: 3503 3097 2531 1009 673 324 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2025 2057 2690 2722 2785 Size: 32 135 32 453 32 633 32 63 Cuts at: 2785 2817 3655 3687 3773 3805 3833 3865 4070 Size: 32 838 32 86 32 28 32 205 Cuts at: 4070 4102 4666 4698 4858 4890 5570 5602 5988 Size: 32 564 32 160 32 680 32 386 Cuts at: 5988 6020 6604 6636 8053 8085 9778 9810 9852 Size: 32 584 32 1417 32 1693 32 42 Cuts at: 9852 9884 1373 Size: 32 2626 Fragments arranged by size: 2626 1693 1417 838 680 633 584 564 453 386 205 160 135 86 63 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 5080 7806 8481 8727 669 Size: 988 281 3142 2726 675 246 3079 Fragments arranged by size: 3142 3079 2726 988 675 281 246 HindIII A'AGCT_T Cuts at: 748 4220 5381 6388 6753 7028 748 Size: 3472 1161 1007 365 275 4857 Fragments arranged by size: 4857 3472 1161 1007 365 275 HpaI GTT'AAC Cuts at: 8727 8727 Size: 11137 KasI G'GCGC_C Cuts at: 6186 6186 Size: 11137 KpnI G_GTAC'C Cuts at: 8462 8462 Size: 11137 MfeI C'AATT_G Cuts at: 8736 8736 Size: 11137 MluI A'CGCG_T Cuts at: 11013 11013 Size: 11137 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2253 5581 7651 10251 10435 1305 Size: 948 3328 2070 2600 184 2007 Fragments arranged by size: 3328 2600 2070 2007 948 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3497 6953 7190 8168 10 Size: 54 1425 343 1665 3456 237 978 2979 Fragments arranged by size: 3456 2979 1665 1425 978 343 237 54 MslI CAynn'nnrTG Cuts at: 518 1166 2338 4565 5156 5910 6809 7091 9095 Size: 648 1172 2227 591 754 899 282 2004 Cuts at: 9095 9454 9613 518 Size: 359 159 2042 Fragments arranged by size: 2227 2042 2004 1172 899 754 648 591 359 282 159 MspA1I CmG'CkG Cuts at: 1606 2026 3743 5156 8083 8553 9230 10378 10623 Size: 420 1717 1413 2927 470 677 1148 245 Cuts at: 10623 1606 Size: 2120 Fragments arranged by size: 2927 2120 1717 1413 1148 677 470 420 245 NaeI GCC'GGC Cuts at: 7870 7870 Size: 11137 NarI GG'CG_CC Cuts at: 6187 6187 Size: 11137 NcoI C'CATG_G Cuts at: 513 1065 2823 5175 6696 7710 8169 513 Size: 552 1758 2352 1521 1014 459 3481 Fragments arranged by size: 3481 2352 1758 1521 1014 552 459 NdeI CA'TA_TG Cuts at: 387 387 Size: 11137 NgoMIV G'CCGG_C Cuts at: 7868 7868 Size: 11137 NheI G'CTAG_C Cuts at: 1052 2221 3194 1052 Size: 1169 973 8995 Fragments arranged by size: 8995 1169 973 NotI GC'GGCC_GC Cuts at: 8584 8584 Size: 11137 NsiI A_TGCA'T Cuts at: 8150 8150 Size: 11137 NspI r_CATG'y Cuts at: 1132 1229 3055 8495 10040 1132 Size: 97 1826 5440 1545 2229 Fragments arranged by size: 5440 2229 1826 1545 97 PacI TTA_AT'TAA Cuts at: 3756 3756 Size: 11137 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 11137 PciI A'CATG_T Cuts at: 10036 10036 Size: 11137 PflMI CCAn_nnn'nTGG Cuts at: 1108 3190 5813 1108 Size: 2082 2623 6432 Fragments arranged by size: 6432 2623 2082 PfoI T'CCnGG_A Cuts at: 5634 6174 6899 7617 8115 5634 Size: 540 725 718 498 8656 Fragments arranged by size: 8656 725 718 540 498 PmeI GTTT'AAAC Cuts at: 8441 8441 Size: 11137 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5834 5866 9206 9238 10719 10751 5834 Size: 32 3340 32 1481 32 6220 Fragments arranged by size: 6220 3340 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 11137 PsiI TTA'TAA Cuts at: 5261 8036 8707 5261 Size: 2775 671 7691 Fragments arranged by size: 7691 2775 671 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2524 2556 4788 4820 7985 8017 2524 Size: 32 2232 32 3165 32 5644 Fragments arranged by size: 5644 3165 2232 32 32 32 PstI C_TGCA'G Cuts at: 830 2698 2931 4243 4655 4674 5649 6888 7299 Size: 1868 233 1312 412 19 975 1239 411 Cuts at: 7299 8489 830 Size: 1190 3478 Fragments arranged by size: 3478 1868 1312 1239 1190 975 412 411 233 19 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11137 PvuII CAG'CTG Cuts at: 2026 5156 8083 2026 Size: 3130 2927 5080 Fragments arranged by size: 5080 3130 2927 SacI G_AGCT'C Cuts at: 4329 7584 4329 Size: 3255 7882 SalI G'TCGA_C Cuts at: 1936 8479 1936 Size: 6543 4594 SapI GCTCTTCn'nnn_ Cuts at: 4317 7572 4317 Size: 3255 7882 SbfI CC_TGCA'GG Cuts at: 8489 8489 Size: 11137 ScaI AGT'ACT Cuts at: 5067 6410 9385 11097 5067 Size: 1343 2975 1712 5107 Fragments arranged by size: 5107 2975 1712 1343 SexAI A'CCwGG_T Cuts at: 7905 7905 Size: 11137 SfcI C'TryA_G Cuts at: 826 1045 2694 2927 4239 4651 4670 4955 5645 Size: 219 1649 233 1312 412 19 285 690 Cuts at: 5645 6099 6494 6884 7295 8485 9620 10301 10492 Size: 454 395 390 411 1190 1135 681 191 Cuts at: 10492 826 Size: 1471 Fragments arranged by size: 1649 1471 1312 1190 1135 690 681 454 412 411 395 390 285 233 219 191 19 SfoI GGC'GCC Cuts at: 6188 6188 Size: 11137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11137 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11137 SmaI CCC'GGG Cuts at: 1872 2625 8464 1872 Size: 753 5839 4545 Fragments arranged by size: 5839 4545 753 SmlI C'TyrA_G Cuts at: 820 1017 1943 2324 3731 4677 9241 10142 10404 Size: 197 926 381 1407 946 4564 901 262 Cuts at: 10404 820 Size: 1553 Fragments arranged by size: 4564 1553 1407 946 926 901 381 262 197 SnaBI TAC'GTA Cuts at: 493 8928 493 Size: 8435 2702 SpeI A'CTAG_T Cuts at: 152 7382 152 Size: 7230 3907 SphI G_CATG'C Cuts at: 1132 8495 1132 Size: 7363 3774 SspI AAT'ATT Cuts at: 5 52 2433 5946 9061 5 Size: 47 2381 3513 3115 2081 Fragments arranged by size: 3513 3115 2381 2081 47 StuI AGG'CCT Cuts at: 1517 6639 1517 Size: 5122 6015 StyI C'CwwG_G Cuts at: 513 1065 2232 2823 2950 5175 5523 6696 7284 Size: 552 1167 591 127 2225 348 1173 588 Cuts at: 7284 7710 8169 8576 513 Size: 426 459 407 3074 Fragments arranged by size: 3074 2225 1173 1167 591 588 552 459 426 407 348 127 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4064 6212 9164 9181 9334 9519 9964 Size: 1255 2484 2148 2952 17 153 185 445 Cuts at: 9964 325 Size: 1498 Fragments arranged by size: 2952 2484 2148 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2150 5065 6408 Size: 274 80 33 51 1443 173 2915 1343 Cuts at: 6408 9383 11095 96 Size: 2975 1712 138 Fragments arranged by size: 2975 2915 1712 1443 1343 274 173 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7449 7481 11097 11129 7449 Size: 32 3616 32 7457 Fragments arranged by size: 7457 3616 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11137 XbaI T'CTAG_A Cuts at: 2069 4573 5248 5581 8473 2069 Size: 2504 675 333 2892 4733 Fragments arranged by size: 4733 2892 2504 675 333 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4302 4838 4302 Size: 536 10601 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 11137 XmaI C'CCGG_G Cuts at: 1870 2623 8462 1870 Size: 753 5839 4545 Fragments arranged by size: 5839 4545 753 XmnI GAAnn'nnTTC Cuts at: 3276 4556 5370 5589 6444 7347 8322 9266 3276 Size: 1280 814 219 855 903 975 944 5147 Fragments arranged by size: 5147 1280 975 944 903 855 814 219 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2010 8945 276 Size: 53 83 186 936 476 6935 2468 Fragments arranged by size: 6935 2468 936 476 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BsiWI EcoNI EcoRV FseI FspAI NruI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SfiI SrfI SwaI