Restriction map for
pFN21AB9463
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# NO CUT ENZYMES ( 73 enzymes) #
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AatII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp700I AspEI AspI BbeI BglII
BsaBI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspEI BspHI BsrFI BsrGI
BssHII Cfr10I CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI KasI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SfiI SfoI SgrAI SnaBI
SpeI SphI SspBI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 4167 GGTACC G^GTACC
AcyI 4924 GGCGTC G[GA]^CG[CT]C
AflII 3771 CTTAAG C^TTAAG
AseI 367 ATTAAT AT^TAAT
AsnI 367 ATTAAT AT^TAAT
Asp718I 4167 GGTACC G^GTACC
AvrII 3627 CCTAGG C^CTAGG
BamHI 3572 GGATCC G^GATCC
BbvCI 4093 GCTGAGG CCTCAGC(-5/-2)
BciVI 5467 GTATCC GTATCC(6/5)
BfrI 3771 CTTAAG C^TTAAG
BglI 2809 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3627 CCTAGG C^CTAGG
BsaHI 4924 GGCGTC G[GA]^CG[CT]C
BssSI 4308 CACGAG CACGAG(-5/-1)
Bst1107I 3949 GTATAC GTA^TAC
BstBI 4547 TTCGAA TT^CGAA
BstZ17I 3949 GTATAC GTA^TAC
EcoNI 2741 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2826 GAATTC G^AATTC
Hin1I 4924 GGCGTC G[GA]^CG[CT]C
HpaI 2260 GTTAAC GTT^AAC
KpnI 4167 GGTACC GGTAC^C
KspI 5266 CCGCGG CCGC^GG
NdeI 1555 CATATG CA^TATG
NspV 4547 TTCGAA TT^CGAA
PshAI 4576 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 367 ATTAAT AT^TAAT
SacI 5259 GAGCTC GAGCT^C
SacII 5266 CCGCGG CCGC^GG
SbfI 3585 CCTGCAGG CCTGCA^GG
SexAI 2792 ACCAGGT A^CC[AT]GGT
SfuI 4547 TTCGAA TT^CGAA
SmaI 1613 CCCGGG CCC^GGG
SmlI 3771 CTTAAG C^T[CT][GA]AG
Sse8387I 3585 CCTGCAGG CCTGCA^GG
XcmI 4307 CCACGAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1613 CCCGGG C^CCGGG
XmaI 1613 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AviII 1116 TGCGCA TGC^GCA
3290 TGCGCA
BbrPI 226 CACGTG CAC^GTG
1030 CACGTG
BcgI 3007 CGAGTCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5331 CGATGAAGATGC
BclI 2157 TGATCA T^GATCA
4476 TGATCA
BsaAI 226 CACGTG [CT]AC^GT[GA]
1030 CACGTG
BsmI 5510 GAATGC GAATGC(1/-1)
3185 GCATTC
BspDI 874 ATCGAT AT^CGAT
4405 ATCGAT
BspLU11I 1305 ACATGT A^CATGT
2066 ACATGT
BstEII 2031 GGTTACC G^GT[ACGT]ACC
2457 GGTCACC
BstPI 2031 GGTTACC G^GT[ACGT]ACC
2457 GGTCACC
ClaI 874 ATCGAT AT^CGAT
4405 ATCGAT
EcoO65I 2031 GGTTACC G^GT[ACGT]ACC
2457 GGTCACC
FbaI 2157 TGATCA T^GATCA
4476 TGATCA
FspI 1116 TGCGCA TGC^GCA
3290 TGCGCA
HaeII 1833 GGCGCT [GA]GCGC^[CT]
3029 AGCGCC
HgaI 2406 GCGTC GACGC(5/10)
4925 GCGTC
PmaCI 226 CACGTG CAC^GTG
1030 CACGTG
PmlI 226 CACGTG CAC^GTG
1030 CACGTG
PpuMI 259 GGGTCCT [GA]G^G[AT]CC[CT]
2594 AGGACCT
SalI 2428 GTCGAC G^TCGAC
3911 GTCGAC
SapI 5233 GAAGAGC GCTCTTC(1/4)
298 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII