Restriction map for
pFN21AB9463
(Linear) (Six-base) MAPSORT of: KIBB9463.seq Check: 2743 from: 1 to: 5616
KIBB9463.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1416 3022 3143 5616
Size: 1416 1606 121 2473
Fragments arranged by size:
2473 1606 1416 121
AccI GT'mk_AC
Cuts at: 0 2429 3912 3950 5616
Size: 2429 1483 38 1666
Fragments arranged by size:
2429 1666 1483 38
Acc65I G'GTAC_C
Cuts at: 0 4167 5616
Size: 4167 1449
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 619 2263 2458 2688 3175 3359 3630
Size: 172 447 1644 195 230 487 184 271
Cuts at: 3630 4473 5048 5495 5616
Size: 843 575 447 121
Fragments arranged by size:
1644 843 575 487 447 447 271 230
195 184 172 121
AflII C'TTAA_G
Cuts at: 0 3771 5616
Size: 3771 1845
AflIII A'CryG_T
Cuts at: 0 1305 2066 3881 5616
Size: 1305 761 1815 1735
Fragments arranged by size:
1815 1735 1305 761
AleI CACnn'nnGTG
Cuts at: 0 1133 3523 5616
Size: 1133 2390 2093
Fragments arranged by size:
2390 2093 1133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 734 1625 1772 3541 5616
Size: 734 891 147 1769 2075
Fragments arranged by size:
2075 1769 891 734 147
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3269 3301 5616
Size: 3269 32 2315
Fragments arranged by size:
3269 2315 32
AlwNI CAG_nnn'CTG
Cuts at: 0 246 1288 1771 2640 3896 5616
Size: 246 1042 483 869 1256 1720
Fragments arranged by size:
1720 1256 1042 869 483 246
ApoI r'AATT_y
Cuts at: 0 2826 3214 3356 3682 3985 4086 4535 4544
Size: 2826 388 142 326 303 101 449 9
Cuts at: 4544 5616
Size: 1072
Fragments arranged by size:
2826 1072 449 388 326 303 142 101
9
AseI AT'TA_AT
Cuts at: 0 368 5616
Size: 368 5248
AvaI C'yCGr_G
Cuts at: 0 1415 1613 2927 3431 5616
Size: 1415 198 1314 504 2185
Fragments arranged by size:
2185 1415 1314 504 198
AvrII C'CTAG_G
Cuts at: 0 3627 5616
Size: 3627 1989
BamHI G'GATC_C
Cuts at: 0 3572 5616
Size: 3572 2044
BanI G'GyrC_C
Cuts at: 0 964 1065 1459 3525 4167 5616
Size: 964 101 394 2066 642 1449
Fragments arranged by size:
2066 1449 964 642 394 101
BanII G_rGCy'C
Cuts at: 0 1185 2966 5240 5263 5616
Size: 1185 1781 2274 23 353
Fragments arranged by size:
2274 1781 1185 353 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 3401 3537 4895 5616
Size: 3401 136 1358 721
Fragments arranged by size:
3401 1358 721 136
BbvCI CC'TCA_GC
Cuts at: 0 4094 5616
Size: 4094 1522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2996 3030 5320 5354 5616
Size: 2996 34 2290 34 262
Fragments arranged by size:
2996 2290 262 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5478 5616
Size: 5478 138
BclI T'GATC_A
Cuts at: 0 2157 4476 5616
Size: 2157 2319 1140
Fragments arranged by size:
2319 2157 1140
BglI GCCn_nnn'nGGC
Cuts at: 0 2815 5616
Size: 2815 2801
BlpI GC'TnA_GC
Cuts at: 0 2306 2402 4950 5616
Size: 2306 96 2548 666
Fragments arranged by size:
2548 2306 666 96
Bme1580I G_kGCm'C
Cuts at: 0 969 1464 2021 2851 3530 3561 5616
Size: 969 495 557 830 679 31 2055
Fragments arranged by size:
2055 969 830 679 557 495 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 2617 2780 3807 5616
Size: 2617 163 1027 1809
Fragments arranged by size:
2617 1809 1027 163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5277 5616
Size: 5277 339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1352 1867 2326 4696 5057 5164 5616
Size: 496 856 515 459 2370 361 107 452
Fragments arranged by size:
2370 856 515 496 459 452 361 107
Bpu10I CC'TnA_GC
Cuts at: 0 1671 2839 4094 5616
Size: 1671 1168 1255 1522
Fragments arranged by size:
1671 1522 1255 1168
BsaI GGTCTCn'nnnn_
Cuts at: 0 1019 2723 4687 4849 5308 5616
Size: 1019 1704 1964 162 459 308
Fragments arranged by size:
1964 1704 1019 459 308 162
BsaAI yAC'GTr
Cuts at: 0 228 1032 5616
Size: 228 804 4584
Fragments arranged by size:
4584 804 228
BsaHI Gr'CG_yC
Cuts at: 0 4925 5616
Size: 4925 691
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 758 788 1016 1046 2243 2273 3036 3066
Size: 758 30 228 30 1197 30 763 30
Cuts at: 3066 3132 3162 5158 5188 5616
Size: 66 30 1996 30 428
Fragments arranged by size:
1996 1197 763 758 428 228 66 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 485 794 2467 3137 4543 4810 4843
Size: 78 407 309 1673 670 1406 267 33
Cuts at: 4843 5176 5179 5269 5616
Size: 333 3 90 347
Fragments arranged by size:
1673 1406 670 407 347 333 309 267
90 78 33 3
BseYI C'CCAG_C
Cuts at: 0 249 2626 2843 3195 5116 5125 5616
Size: 249 2377 217 352 1921 9 491
Fragments arranged by size:
2377 1921 491 352 249 217 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 704 902 1189 1585 3556 4793 5524
Size: 259 445 198 287 396 1971 1237 731
Cuts at: 5524 5616
Size: 92
Fragments arranged by size:
1971 1237 731 445 396 287 259 198
92
BsiHKAI G_wGCw'C
Cuts at: 0 472 3114 5249 5263 5616
Size: 472 2642 2135 14 353
Fragments arranged by size:
2642 2135 472 353 14
BsmI GAATG_Cn'
Cuts at: 0 3185 5516 5616
Size: 3185 2331 100
Fragments arranged by size:
3185 2331 100
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1387 2503 4884 5351 5616
Size: 1387 1116 2381 467 265
Fragments arranged by size:
2381 1387 1116 467 265
Bsp1286I G_dGCh'C
Cuts at: 0 472 969 1185 1464 2021 2851 2966 3114
Size: 472 497 216 279 557 830 115 148
Cuts at: 3114 3530 3561 5240 5249 5263 5616
Size: 416 31 1679 9 14 353
Fragments arranged by size:
1679 830 557 497 472 416 353 279
216 148 115 31 14 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 184 1416 2666 3022 3143 4867 5030 5616
Size: 184 1232 1250 356 121 1724 163 586
Fragments arranged by size:
1724 1250 1232 586 356 184 163 121
BsrBI CCG'CTC
Cuts at: 0 298 1797 2305 5202 5616
Size: 298 1499 508 2897 414
Fragments arranged by size:
2897 1499 508 414 298
BsrDI GCAATG_nn'
Cuts at: 0 99 305 1208 5616
Size: 99 206 903 4408
Fragments arranged by size:
4408 903 206 99
BssSI C'ACGA_G
Cuts at: 0 4308 5616
Size: 4308 1308
BstAPI GCAn_nnn'nTGC
Cuts at: 0 246 1771 5022 5511 5616
Size: 246 1525 3251 489 105
Fragments arranged by size:
3251 1525 489 246 105
BstBI TT'CG_AA
Cuts at: 0 4548 5616
Size: 4548 1068
BstEII G'GTnAC_C
Cuts at: 0 2031 2457 5616
Size: 2031 426 3159
Fragments arranged by size:
3159 2031 426
BstXI CCAn_nnnn'nTGG
Cuts at: 0 546 672 4761 5616
Size: 546 126 4089 855
Fragments arranged by size:
4089 855 546 126
BstYI r'GATC_y
Cuts at: 0 3239 3572 3623 3917 5616
Size: 3239 333 51 294 1699
Fragments arranged by size:
3239 1699 333 294 51
BstZ17I GTA'TAC
Cuts at: 0 3951 5616
Size: 3951 1665
Bsu36I CC'TnA_GG
Cuts at: 0 2584 2591 2746 5138 5616
Size: 2584 7 155 2392 478
Fragments arranged by size:
2584 2392 478 155 7
BtgI C'CryG_G
Cuts at: 0 2423 5266 5426 5616
Size: 2423 2843 160 190
Fragments arranged by size:
2843 2423 190 160
BtsI GCAGTG_nn'
Cuts at: 0 470 1201 1597 1871 2369 2384 3300 3461
Size: 470 731 396 274 498 15 916 161
Cuts at: 3461 4805 5616
Size: 1344 811
Fragments arranged by size:
1344 916 811 731 498 470 396 274
161 15
ClaI AT'CG_AT
Cuts at: 0 875 4406 5616
Size: 875 3531 1210
Fragments arranged by size:
3531 1210 875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1289 1324 5616
Size: 1289 35 4292
Fragments arranged by size:
4292 1289 35
DraIII CAC_nnn'GTG
Cuts at: 0 925 2796 5427 5616
Size: 925 1871 2631 189
Fragments arranged by size:
2631 1871 925 189
EaeI y'GGCC_r
Cuts at: 0 536 1125 1574 3077 3980 4100 4185 5616
Size: 536 589 449 1503 903 120 85 1431
Fragments arranged by size:
1503 1431 903 589 536 449 120 85
EarI CTCTTCn'nnn_
Cuts at: 0 249 305 755 2537 4394 4574 5024 5228
Size: 249 56 450 1782 1857 180 450 204
Cuts at: 5228 5486 5575 5616
Size: 258 89 41
Fragments arranged by size:
1857 1782 450 450 258 249 204 180
89 56 41
EcoICRI GAG'CTC
Cuts at: 0 5261 5616
Size: 5261 355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 496 619 1352 1867 2263 2326 2458
Size: 172 324 123 733 515 396 63 132
Cuts at: 2458 2688 3175 3359 3630 4473 4696 5048 5057
Size: 230 487 184 271 843 223 352 9
Cuts at: 5057 5164 5495 5616
Size: 107 331 121
Fragments arranged by size:
843 733 515 487 396 352 331 324
271 230 223 184 172 132 123 121
107 63 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2745 5616
Size: 2745 2871
EcoO109I rG'GnC_Cy
Cuts at: 0 260 2595 3199 5616
Size: 260 2335 604 2417
Fragments arranged by size:
2417 2335 604 260
EcoRI G'AATT_C
Cuts at: 0 2826 5616
Size: 2826 2790
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2846 4481 5616
Size: 2846 1635 1135
Fragments arranged by size:
2846 1635 1135
FspI TGC'GCA
Cuts at: 0 1118 3292 5616
Size: 1118 2174 2324
Fragments arranged by size:
2324 2174 1118
HaeII r_GCGC'y
Cuts at: 0 1837 3033 5616
Size: 1837 1196 2583
Fragments arranged by size:
2583 1837 1196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 601 633 646 678 1152 1184 1621 1653
Size: 601 32 13 32 474 32 437 32
Cuts at: 1653 2045 2077 2772 2804 3894 3926 3947 3979
Size: 392 32 695 32 1090 32 21 32
Cuts at: 3979 4288 4320 4399 4431 4848 4880 5616
Size: 309 32 79 32 417 32 736
Fragments arranged by size:
1090 736 695 601 474 437 417 392
309 79 32 32 32 32 32 32
32 32 32 32 32 21 13
HincII GTy'rAC
Cuts at: 0 738 899 2072 2232 2262 2430 2801 3913
Size: 738 161 1173 160 30 168 371 1112
Cuts at: 3913 5529 5616
Size: 1616 87
Fragments arranged by size:
1616 1173 1112 738 371 168 161 160
87 30
HindIII A'AGCT_T
Cuts at: 0 99 2176 3722 5616
Size: 99 2077 1546 1894
Fragments arranged by size:
2077 1894 1546 99
HpaI GTT'AAC
Cuts at: 0 2262 5616
Size: 2262 3354
KpnI G_GTAC'C
Cuts at: 0 4171 5616
Size: 4171 1445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 935 2735 3025 3078 5616
Size: 935 1800 290 53 2538
Fragments arranged by size:
2538 1800 935 290 53
MscI TGG'CCA
Cuts at: 0 538 1127 3079 3982 4102 4187 5616
Size: 538 589 1952 903 120 85 1429
Fragments arranged by size:
1952 1429 903 589 538 120 85
MslI CAynn'nnrTG
Cuts at: 0 428 1133 1568 2026 2422 3326 3523 4733
Size: 428 705 435 458 396 904 197 1210
Cuts at: 4733 5616
Size: 883
Fragments arranged by size:
1210 904 883 705 458 435 428 396
197
MspA1I CmG'CkG
Cuts at: 0 1542 3195 3311 4493 5268 5616
Size: 1542 1653 116 1182 775 348
Fragments arranged by size:
1653 1542 1182 775 348 116
NdeI CA'TA_TG
Cuts at: 0 1556 5616
Size: 1556 4060
NspI r_CATG'y
Cuts at: 0 814 1309 1705 2070 4627 5616
Size: 814 495 396 365 2557 989
Fragments arranged by size:
2557 989 814 495 396 365
PasI CC'CwG_GG
Cuts at: 0 1020 5616
Size: 1020 4596
PciI A'CATG_T
Cuts at: 0 1305 2066 5616
Size: 1305 761 3550
Fragments arranged by size:
3550 1305 761
PflMI CCAn_nnn'nTGG
Cuts at: 0 534 942 1830 3039 3896 5616
Size: 534 408 888 1209 857 1720
Fragments arranged by size:
1720 1209 888 857 534 408
PfoI T'CCnGG_A
Cuts at: 0 2639 3204 3920 4712 5073 5616
Size: 2639 565 716 792 361 543
Fragments arranged by size:
2639 792 716 565 543 361
PmlI CAC'GTG
Cuts at: 0 228 1032 5616
Size: 228 804 4584
Fragments arranged by size:
4584 804 228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1348 1380 5616
Size: 1348 32 4236
Fragments arranged by size:
4236 1348 32
PpuMI rG'GwC_Cy
Cuts at: 0 260 2595 5616
Size: 260 2335 3021
Fragments arranged by size:
3021 2335 260
PshAI GACnn'nnGTC
Cuts at: 0 4580 5616
Size: 4580 1036
PsiI TTA'TAA
Cuts at: 0 5563 5616
Size: 5563 53
PstI C_TGCA'G
Cuts at: 0 1355 1776 3590 5160 5616
Size: 1355 421 1814 1570 456
Fragments arranged by size:
1814 1570 1355 456 421
PvuII CAG'CTG
Cuts at: 0 1542 3195 3311 4493 5616
Size: 1542 1653 116 1182 1123
Fragments arranged by size:
1653 1542 1182 1123 116
SacI G_AGCT'C
Cuts at: 0 5263 5616
Size: 5263 353
SacII CC_GC'GG
Cuts at: 0 5269 5616
Size: 5269 347
SalI G'TCGA_C
Cuts at: 0 2428 3911 5616
Size: 2428 1483 1705
Fragments arranged by size:
2428 1705 1483
SapI GCTCTTCn'nnn_
Cuts at: 0 305 5228 5616
Size: 305 4923 388
Fragments arranged by size:
4923 388 305
SbfI CC_TGCA'GG
Cuts at: 0 3590 5616
Size: 3590 2026
ScaI AGT'ACT
Cuts at: 0 3380 3448 4600 5616
Size: 3380 68 1152 1016
Fragments arranged by size:
3380 1152 1016 68
SexAI A'CCwGG_T
Cuts at: 0 2792 5616
Size: 2792 2824
SfcI C'TryA_G
Cuts at: 0 476 1351 1772 3586 3653 4628 5156 5616
Size: 476 875 421 1814 67 975 528 460
Fragments arranged by size:
1814 975 875 528 476 460 421 67
SmaI CCC'GGG
Cuts at: 0 1615 5616
Size: 1615 4001
SmlI C'TyrA_G
Cuts at: 0 3771 5616
Size: 3771 1845
SspI AAT'ATT
Cuts at: 0 366 442 5059 5616
Size: 366 76 4617 557
Fragments arranged by size:
4617 557 366 76
StuI AGG'CCT
Cuts at: 0 2589 3074 3584 4316 5616
Size: 2589 485 510 732 1300
Fragments arranged by size:
2589 1300 732 510 485
StyI C'CwwG_G
Cuts at: 0 215 2390 2810 3120 3219 3627 4482 5616
Size: 215 2175 420 310 99 408 855 1134
Fragments arranged by size:
2175 1134 855 420 408 310 215 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2599 5616
Size: 2599 3017
TatI w'GTAC_w
Cuts at: 0 2992 3378 3446 3507 4598 5616
Size: 2992 386 68 61 1091 1018
Fragments arranged by size:
2992 1091 1018 386 68 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 758 790 1016 1048 5411 5443 5616
Size: 758 32 226 32 4363 32 173
Fragments arranged by size:
4363 758 226 173 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4314 5616
Size: 4314 1302
XmaI C'CCGG_G
Cuts at: 0 1613 5616
Size: 1613 4003
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AleI AlfI
AloI AlwNI ApoI AseI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MmeI
MscI MslI MspA1I NdeI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PsiI PstI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SmaI SmlI SspI StuI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AgeI AhdI ApaI ApaLI AscI
AsiSI BaeI BbeI BfrBI BglII BmgBI BmtI BpuEI
BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrFI BsrGI
BssHII BtgZI DraI DrdI EagI EciI EcoRV FseI
FspAI KasI MfeI MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PmeI PspOMI PspXI
PsrI PvuI RsrII SanDI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI
XmnI ZraI