Restriction map for
pFN21AB9460
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflIII AgeI AhdI Alw44I
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvrII
BbrPI BbvCI BcgI BciVI BlnI
BsaBI BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FseI HincII HindII HpaI
KpnI MamI MluI MroI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacI
SalI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflII 1336 CTTAAG C^TTAAG
AscI 579 GGCGCGCC GG^CGCGCC
AviII 1709 TGCGCA TGC^GCA
BalI 947 TGGCCA TGG^CCA
BamHI 1559 GGATCC G^GATCC
BbsI 1138 GTCTTC GAAGAC(2/6)
BclI 1130 TGATCA T^GATCA
BfrI 1336 CTTAAG C^TTAAG
BglII 1466 AGATCT A^GATCT
BlpI 1905 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1905 GCTGAGC GC^T[ACGT]AGC
BpuAI 1138 GTCTTC GAAGAC(2/6)
BsaAI 1867 TACGTG [CT]AC^GT[GA]
BsaI 1721 GAGACC GGTCTC(1/5)
BsrDI 1593 CATTGC GCAATG(2/0)
CelII 1905 GCTGAGC GC^T[ACGT]AGC
CpoI 725 CGGTCCG CG^G[AT]CCG
DraIII 2140 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1291 GACCAGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 920 CCAAGG C^C[AT][AT]GG
FbaI 1130 TGATCA T^GATCA
FspI 1709 TGCGCA TGC^GCA
HindIII 388 AAGCTT A^AGCTT
MfeI 1439 CAATTG C^AATTG
MluNI 947 TGGCCA TGG^CCA
MscI 947 TGGCCA TGG^CCA
MunI 1439 CAATTG C^AATTG
PflMI 678 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 725 CGGTCCG CG^G[AT]CCG
SbfI 972 CCTGCAGG CCTGCA^GG
SfiI 608 GGCCCGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2154 CCCGGG CCC^GGG
Sse8387I 972 CCTGCAGG CCTGCA^GG
StyI 920 CCAAGG C^C[AT][AT]GG
TaqI 1667 TCGA T^CGA
TfiI 427 GAATC G^A[AT]TC
Tsp509I 1440 AATT ^AATT
TthHB8I 1667 TCGA T^CGA
Van91I 678 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2154 CCCGGG C^CCGGG
XmaI 2154 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AocI 10 CCTGAGG CC^T[ACGT]AGG
1104 CCTCAGG
BfaI 1057 CTAG C^TAG
1073 CTAG
BsiEI 55 CGGCCG CG[GA][CT]^CG
572 CGGCCG
BstAPI 1629 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2139 GCACCCAGTGC
BstXI 938 CCACAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1143 CCACCGAGATGG
Bsu36I 10 CCTGAGG CC^T[ACGT]AGG
1104 CCTCAGG
EagI 55 CGGCCG C^GGCCG
572 CGGCCG
EclXI 55 CGGCCG C^GGCCG
572 CGGCCG
Eco52I 55 CGGCCG C^GGCCG
572 CGGCCG
Eco81I 10 CCTGAGG CC^T[ACGT]AGG
1104 CCTCAGG
EcoNI 313 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
591 CCTGCGGGAGG
KspI 602 CCGCGG CCGC^GG
722 CCGCGG
MaeI 1057 CTAG C^TAG
1073 CTAG
MslI 216 CACCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1144 CACCGAGATG
NaeI 618 GCCGGC GCC^GGC
930 GCCGGC
NgoMIV 618 GCCGGC G^CCGGC
930 GCCGGC
PleI 1488 GACTC GAGTC(4/5)
1222 GAGTC
PpuMI 62 AGGACCT [GA]G^G[AT]CC[CT]
717 GGGTCCC
PstI 973 CTGCAG CTGCA^G
1420 CTGCAG
PvuII 1633 CAGCTG CAG^CTG
1705 CAGCTG
SacII 602 CCGCGG CCGC^GG
722 CCGCGG
SapI 877 GAAGAGC GCTCTTC(1/4)
1474 GAAGAGC
SfcI 973 CTGCAG C^T[GA][CT]AG
1420 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssHII BssKI BssSI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfoI SmlI StuI Tru9I TseI
Tsp45I TspRI XhoII