(Circular) (Six-base) MAPSORT of: pFN21AB9456.seq Check: 2766 from: 1 to: 8155 pFN21AB9456 8155 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5965 278 Size: 53 83 186 936 4429 2468 Fragments arranged by size: 4429 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2577 2590 2964 5498 1937 Size: 640 13 374 2534 4594 Fragments arranged by size: 4594 2534 640 374 13 Acc65I G'GTAC_C Cuts at: 2561 2828 5476 2561 Size: 267 2648 5240 Fragments arranged by size: 5240 2648 267 AclI AA'CG_TT Cuts at: 1563 6282 6655 1563 Size: 4719 373 3063 Fragments arranged by size: 4719 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2022 2544 2642 3372 3743 6218 7602 7870 Size: 390 522 98 730 371 2475 1384 268 Cuts at: 7870 1632 Size: 1917 Fragments arranged by size: 2475 1917 1384 730 522 390 371 268 98 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7958 AflIII A'CryG_T Cuts at: 1203 3743 4312 7054 8031 1203 Size: 2540 569 2742 977 1327 Fragments arranged by size: 2742 2540 1327 977 569 AgeI A'CCGG_T Cuts at: 6986 6986 Size: 8155 AhdI GACnn_n'nnGTC Cuts at: 6884 6884 Size: 8155 AleI CACnn'nnGTG Cuts at: 1166 4376 4571 1166 Size: 3210 195 4750 Fragments arranged by size: 4750 3210 195 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8155 AlwNI CAG_nnn'CTG Cuts at: 5215 5898 7470 5215 Size: 683 1572 5900 Fragments arranged by size: 5900 1572 683 ApaI G_GGCC'C Cuts at: 2789 4051 2789 Size: 1262 6893 ApaLI G'TGCA_C Cuts at: 3774 5138 6212 7368 3774 Size: 1364 1074 1156 4561 Fragments arranged by size: 4561 1364 1156 1074 ApoI r'AATT_y Cuts at: 1493 2398 5464 5690 5842 7951 1493 Size: 905 3066 226 152 2109 1697 Fragments arranged by size: 3066 2109 1697 905 226 152 AseI AT'TA_AT Cuts at: 160 1032 6709 160 Size: 872 5677 1606 Fragments arranged by size: 5677 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8155 AvaI C'yCGr_G Cuts at: 1870 1943 2127 2145 2775 2895 3238 5175 5480 Size: 73 184 18 630 120 343 1937 305 Cuts at: 5480 1870 Size: 4545 Fragments arranged by size: 4545 1937 630 343 305 184 120 73 18 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4846 4879 4846 Size: 33 8122 BamHI G'GATC_C Cuts at: 2230 5485 5852 2230 Size: 3255 367 4533 Fragments arranged by size: 4533 3255 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2561 2743 2828 2861 3689 Size: 325 217 627 774 182 85 33 828 Cuts at: 3689 5476 6931 618 Size: 1787 1455 1842 Fragments arranged by size: 1842 1787 1455 828 774 627 325 217 182 85 33 BanII G_rGCy'C Cuts at: 1391 2759 2789 4051 4385 5474 1391 Size: 1368 30 1262 334 1089 4072 Fragments arranged by size: 4072 1368 1262 1089 334 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2436 3090 4311 4631 4860 928 Size: 971 537 654 1221 320 229 4223 Fragments arranged by size: 4223 1221 971 654 537 320 229 BbvCI CC'TCA_GC Cuts at: 2108 2108 Size: 8155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6346 6380 1903 Size: 34 50 34 4325 34 3678 Fragments arranged by size: 4325 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4202 4874 6047 7257 4202 Size: 672 1173 1210 5100 Fragments arranged by size: 5100 1210 1173 672 BclI T'GATC_A Cuts at: 1548 5439 1548 Size: 3891 4264 BfrBI ATG'CAT Cuts at: 2008 4939 5257 2008 Size: 2931 318 4906 Fragments arranged by size: 4906 2931 318 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4551 6766 136 Size: 107 122 71 631 605 2879 2215 1525 Fragments arranged by size: 2879 2215 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3518 3526 8150 3518 Size: 8 4624 3523 Fragments arranged by size: 4624 3523 8 BlpI GC'TnA_GC Cuts at: 4434 5572 4434 Size: 1138 7017 Bme1580I G_kGCm'C Cuts at: 1790 2645 2782 2789 3778 4051 5142 6216 7372 Size: 855 137 7 989 273 1091 1074 1156 Cuts at: 7372 1790 Size: 2573 Fragments arranged by size: 2573 1156 1091 1074 989 855 273 137 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8155 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2232 2792 5886 Size: 416 53 83 399 24 815 560 3094 Cuts at: 5886 6844 442 Size: 958 1753 Fragments arranged by size: 3094 1753 958 815 560 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8155 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1998 2670 3105 3543 4039 4288 6815 1239 Size: 759 672 435 438 496 249 2527 2579 Fragments arranged by size: 2579 2527 759 672 496 438 435 249 Bpu10I CC'TnA_GC Cuts at: 2108 2108 Size: 8155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2469 2912 6280 7145 7443 2469 Size: 443 3368 865 298 3181 Fragments arranged by size: 3368 3181 865 443 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4015 4373 6818 882 Size: 617 2516 358 2445 2219 Fragments arranged by size: 2516 2445 2219 617 358 BsaAI yAC'GTr Cuts at: 493 1204 5946 493 Size: 711 4742 2702 Fragments arranged by size: 4742 2702 711 BsaBI GATnn'nnATC Cuts at: 8149 8149 Size: 8155 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4597 5962 6344 6979 Size: 53 83 186 936 3064 1365 382 635 Cuts at: 6979 275 Size: 1451 Fragments arranged by size: 3064 1451 1365 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2101 2244 5230 6588 6986 7260 7407 7818 2101 Size: 143 2986 1358 398 274 147 411 2438 Fragments arranged by size: 2986 2438 1358 411 398 274 147 143 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7930 Fragments arranged by size: 7930 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 3099 4322 1186 Size: 1255 658 1223 5019 Fragments arranged by size: 5019 1255 1223 658 BseYI C'CCAG_C Cuts at: 2750 3133 4703 7358 7854 2750 Size: 383 1570 2655 496 3051 Fragments arranged by size: 3051 2655 1570 496 383 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4233 4840 1163 Size: 573 2497 607 4478 Fragments arranged by size: 4478 2497 607 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5605 6366 7394 1540 Size: 275 186 3604 761 1028 2301 Fragments arranged by size: 3604 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3778 5142 6216 6301 7372 3778 Size: 1364 1074 85 1071 4561 Fragments arranged by size: 4561 1364 1074 1071 85 BsiWI C'GTAC_G Cuts at: 3013 3013 Size: 8155 BsmI GAATG_Cn' Cuts at: 3306 5666 5759 3306 Size: 2360 93 5702 Fragments arranged by size: 5702 2360 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2645 2759 2782 2789 3778 4051 4385 Size: 399 855 114 23 7 989 273 334 Cuts at: 4385 5142 5474 6216 6301 7372 1391 Size: 757 332 742 85 1071 2174 Fragments arranged by size: 2174 1071 989 855 757 742 399 334 332 273 114 85 23 7 BspEI T'CCGG_A Cuts at: 2101 5230 7818 2101 Size: 3129 2588 2438 Fragments arranged by size: 3129 2588 2438 BspHI T'CATG_A Cuts at: 6044 7774 6044 Size: 1730 6425 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5223 5510 844 Size: 4379 287 3489 Fragments arranged by size: 4379 3489 287 BsrBI CCG'CTC Cuts at: 1394 1823 6042 1394 Size: 429 4219 3507 Fragments arranged by size: 4219 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2724 3610 3888 3909 4714 6650 6824 Size: 1183 1476 886 278 21 805 1936 174 Cuts at: 6824 65 Size: 1396 Fragments arranged by size: 1936 1476 1396 1183 886 805 278 174 21 BsrFI r'CCGG_y Cuts at: 1700 1810 6799 6986 1700 Size: 110 4989 187 2869 Fragments arranged by size: 4989 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2693 2850 96 Size: 2597 157 5401 Fragments arranged by size: 5401 2597 157 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8155 BssSI C'ACGA_G Cuts at: 6215 7227 6215 Size: 1012 7143 BstAPI GCAn_nnn'nTGC Cuts at: 794 2222 794 Size: 1428 6727 BstBI TT'CG_AA Cuts at: 4006 6959 4006 Size: 2953 5202 BstEII G'GTnAC_C Cuts at: 1184 3137 1184 Size: 1953 6202 BstXI CCAn_nnnn'nTGG Cuts at: 2032 2212 3711 4541 4711 2032 Size: 180 1499 830 170 5476 Fragments arranged by size: 5476 1499 830 180 170 BstYI r'GATC_y Cuts at: 1972 2230 3518 3526 3922 4830 5485 5852 6237 Size: 258 1288 8 396 908 655 367 385 Cuts at: 6237 6254 7706 7792 7804 8150 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1288 908 655 396 385 367 346 258 86 17 12 8 Bsu36I CC'TnA_GG Cuts at: 5349 5349 Size: 8155 BtgI C'CryG_G Cuts at: 513 1065 3710 4122 4195 513 Size: 552 2645 412 73 4473 Fragments arranged by size: 4473 2645 552 412 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7416 Fragments arranged by size: 7416 683 56 BtsI GCAGTG_nn' Cuts at: 4670 5271 5437 5671 6465 6485 4670 Size: 601 166 234 794 20 6340 Fragments arranged by size: 6340 794 601 234 166 20 ClaI AT'CG_AT Cuts at: 1554 2070 1554 Size: 516 7639 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3571 3606 529 Size: 35 3007 35 5078 Fragments arranged by size: 5078 3007 35 35 DraI TTT'AAA Cuts at: 5459 5459 Size: 8155 DraIII CAC_nnn'GTG Cuts at: 2240 4807 2240 Size: 2567 5588 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4313 7162 809 Size: 507 2997 2849 1802 Fragments arranged by size: 2997 2849 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2463 3072 3701 Size: 54 1425 50 275 18 633 609 629 Cuts at: 3701 3835 4497 5602 6491 8120 8 Size: 134 662 1105 889 1629 43 Fragments arranged by size: 1629 1425 1105 889 662 633 629 609 275 134 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5602 1537 Size: 275 3790 4090 Fragments arranged by size: 4090 3790 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2499 5106 6085 1353 Size: 1146 2607 979 3423 Fragments arranged by size: 3423 2607 1146 979 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4931 5266 6742 7116 7262 387 Size: 1064 138 3342 335 1476 374 146 1280 Fragments arranged by size: 3342 1476 1280 1064 374 335 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1998 2022 2544 2642 2670 3105 3372 Size: 393 366 24 522 98 28 435 267 Cuts at: 3372 3543 3743 4039 4288 6218 6815 7602 7870 Size: 171 200 296 249 1930 597 787 268 Cuts at: 7870 1239 Size: 1524 Fragments arranged by size: 1930 1524 787 597 522 435 393 366 296 268 267 249 200 171 98 28 24 EcoNI CCTnn'n_nnAGG Cuts at: 3362 4457 3362 Size: 1095 7060 EcoO109I rG'GnC_Cy Cuts at: 2275 4048 4247 4879 4995 5212 2275 Size: 1773 199 632 116 217 5218 Fragments arranged by size: 5218 1773 632 217 199 116 EcoRI G'AATT_C Cuts at: 5464 5842 5464 Size: 378 7777 FspI TGC'GCA Cuts at: 6661 6661 Size: 8155 HaeII r_GCGC'y Cuts at: 1678 7302 1678 Size: 5624 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 2889 2921 3158 Size: 32 135 32 524 32 761 32 237 Cuts at: 3158 3190 3360 3392 4060 4092 5054 5086 6796 Size: 32 170 32 668 32 962 32 1710 Cuts at: 6796 6828 6870 6902 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1710 962 761 668 524 237 170 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2703 3442 5290 5499 5745 669 Size: 988 281 765 739 1848 209 246 3079 Fragments arranged by size: 3079 1848 988 765 739 281 246 209 HindIII A'AGCT_T Cuts at: 748 4422 748 Size: 3674 4481 HpaI GTT'AAC Cuts at: 5290 5745 5290 Size: 455 7700 KpnI G_GTAC'C Cuts at: 2565 2832 5480 2565 Size: 267 2648 5240 Fragments arranged by size: 5240 2648 267 MfeI C'AATT_G Cuts at: 2670 5051 5754 2670 Size: 2381 703 5071 Fragments arranged by size: 5071 2381 703 MluI A'CGCG_T Cuts at: 8031 8031 Size: 8155 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7269 7453 1305 Size: 5964 184 2007 Fragments arranged by size: 5964 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2465 3074 3703 3837 10 Size: 54 1425 343 633 609 629 134 4328 Fragments arranged by size: 4328 1425 633 629 609 343 134 54 MslI CAynn'nnrTG Cuts at: 518 1166 2030 2916 3429 3709 4064 4376 4571 Size: 648 864 886 513 280 355 312 195 Cuts at: 4571 4739 4944 4979 6113 6472 6631 518 Size: 168 205 35 1134 359 159 2042 Fragments arranged by size: 2042 1134 886 864 648 513 359 355 312 280 205 195 168 159 35 MspA1I CmG'CkG Cuts at: 1606 2648 2750 4489 5571 6248 7396 7641 1606 Size: 1042 102 1739 1082 677 1148 245 2120 Fragments arranged by size: 2120 1739 1148 1082 1042 677 245 102 NcoI C'CATG_G Cuts at: 513 1065 3710 4122 513 Size: 552 2645 412 4546 Fragments arranged by size: 4546 2645 552 412 NdeI CA'TA_TG Cuts at: 387 387 Size: 8155 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8155 NotI GC'GGCC_GC Cuts at: 5602 5602 Size: 8155 NsiI A_TGCA'T Cuts at: 2010 4941 5259 2010 Size: 2931 318 4906 Fragments arranged by size: 4906 2931 318 NspI r_CATG'y Cuts at: 1132 1229 2959 3133 3627 3747 3987 4316 5017 Size: 97 1730 174 494 120 240 329 701 Cuts at: 5017 5227 5513 7058 1132 Size: 210 286 1545 2229 Fragments arranged by size: 2229 1730 1545 701 494 329 286 240 210 174 120 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8155 PciI A'CATG_T Cuts at: 3743 4312 7054 3743 Size: 569 2742 4844 Fragments arranged by size: 4844 2742 569 PflMI CCAn_nnn'nTGG Cuts at: 1108 2795 4492 1108 Size: 1687 1697 4771 Fragments arranged by size: 4771 1697 1687 PfoI T'CCnGG_A Cuts at: 3310 5208 3310 Size: 1898 6257 PmeI GTTT'AAAC Cuts at: 5459 5459 Size: 8155 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6224 6256 7737 7769 6224 Size: 32 1481 32 6610 Fragments arranged by size: 6610 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 4247 4879 4995 5212 2275 Size: 1972 632 116 217 5218 Fragments arranged by size: 5218 1972 632 217 116 PshAI GACnn'nnGTC Cuts at: 1613 2097 2280 1613 Size: 484 183 7488 Fragments arranged by size: 7488 484 183 PsiI TTA'TAA Cuts at: 5725 5725 Size: 8155 PspOMI G'GGCC_C Cuts at: 2785 4047 2785 Size: 1262 6893 PspXI vC'TCGA_Gb Cuts at: 3238 3238 Size: 8155 PstI C_TGCA'G Cuts at: 830 5507 830 Size: 4677 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8155 PvuII CAG'CTG Cuts at: 2648 2750 4489 2648 Size: 102 1739 6314 Fragments arranged by size: 6314 1739 102 SalI G'TCGA_C Cuts at: 1936 5497 1936 Size: 3561 4594 SbfI CC_TGCA'GG Cuts at: 5507 5507 Size: 8155 ScaI AGT'ACT Cuts at: 6403 8115 6403 Size: 1712 6443 SfcI C'TryA_G Cuts at: 826 1045 5503 6638 7319 7510 826 Size: 219 4458 1135 681 191 1471 Fragments arranged by size: 4458 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8155 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8155 SmaI CCC'GGG Cuts at: 1872 2147 2777 5177 5482 1872 Size: 275 630 2400 305 4545 Fragments arranged by size: 4545 2400 630 305 275 SmlI C'TyrA_G Cuts at: 820 1017 1943 2484 2927 3238 6259 7160 7422 Size: 197 926 541 443 311 3021 901 262 Cuts at: 7422 820 Size: 1553 Fragments arranged by size: 3021 1553 926 901 541 443 311 262 197 SnaBI TAC'GTA Cuts at: 493 5946 493 Size: 5453 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8155 SphI G_CATG'C Cuts at: 1132 3133 5513 1132 Size: 2001 2380 3774 Fragments arranged by size: 3774 2380 2001 SspI AAT'ATT Cuts at: 5 52 2387 2599 6079 5 Size: 47 2335 212 3480 2081 Fragments arranged by size: 3480 2335 2081 212 47 StuI AGG'CCT Cuts at: 1517 4273 1517 Size: 2756 5399 StyI C'CwwG_G Cuts at: 513 1065 2857 3183 3363 3710 3801 4122 4162 Size: 552 1792 326 180 347 91 321 40 Cuts at: 4162 4546 5594 513 Size: 384 1048 3074 Fragments arranged by size: 3074 1792 1048 552 384 347 326 321 180 91 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2251 2449 2561 2623 3703 4027 5079 Size: 1255 671 198 112 62 1080 324 1052 Cuts at: 5079 6182 6199 6352 6537 6982 325 Size: 1103 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1103 1080 1052 671 445 324 198 185 153 112 62 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2693 2850 4149 Size: 274 80 33 51 1443 716 157 1299 Cuts at: 4149 5163 6401 8113 96 Size: 1014 1238 1712 138 Fragments arranged by size: 1712 1443 1299 1238 1014 716 274 157 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8115 8147 8115 Size: 32 8123 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8155 XbaI T'CTAG_A Cuts at: 5491 5491 Size: 8155 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2209 2737 3216 3308 4120 2029 Size: 180 528 479 92 812 6064 Fragments arranged by size: 6064 812 528 479 180 92 XhoI C'TCGA_G Cuts at: 1943 3238 1943 Size: 1295 6860 XmaI C'CCGG_G Cuts at: 1870 2145 2775 5175 5480 1870 Size: 275 630 2400 305 4545 Fragments arranged by size: 4545 2400 630 305 275 XmnI GAAnn'nnTTC Cuts at: 2874 3432 6284 2874 Size: 558 2852 4745 Fragments arranged by size: 4745 2852 558 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5963 276 Size: 53 83 186 936 4429 2468 Fragments arranged by size: 4429 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BplI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI