Restriction map for
pFN21AB9448
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MfeI MflI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI StuI
SwaI XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 543 CGCG CG^CG
AcsI 743 AAATTC [GA]^AATT[CT]
ApoI 743 AAATTC [GA]^AATT[CT]
AspI 220 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 20 GGACC G^G[AT]CC
BalI 683 TGGCCA TGG^CCA
BlpI 1332 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1332 GCTGAGC GC^T[ACGT]AGC
BsaBI 718 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 406 GTGCAG GTGCAG(16/14)
BsmI 1280 GAATGC GAATGC(1/-1)
BspHI 813 TCATGA T^CATGA
BsrBI 610 GAGCGG CCGCTC(-3/-3)
BsrDI 353 GCAATG GCAATG(2/0)
BssSI 163 CTCGTG CACGAG(-5/-1)
BstUI 543 CGCG CG^CG
CelII 1332 GCTGAGC GC^T[ACGT]AGC
DraII 19 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 683 TGGCCA [CT]^GGCC[GA]
EcoNI 11 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 19 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 541 CCCGC CCCGC(4/6)
HaeII 567 GGCGCT [GA]GCGC^[CT]
HapII 360 CCGG C^CGG
HgaI 544 GCGTC GACGC(5/10)
HpaII 360 CCGG C^CGG
MamI 718 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 683 TGGCCA TGG^CCA
MscI 683 TGGCCA TGG^CCA
MspA1I 37 CAGCTG C[AC]G^C[GT]G
MspI 360 CCGG C^CGG
MvnI 543 CGCG CG^CG
NheI 175 GCTAGC G^CTAGC
NspI 1164 GCATGC [GA]CATG^[CT]
PaeR7I 914 CTCGAG C^TCGAG
PflFI 220 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 473 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 19 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 37 CAGCTG CAG^CTG
RcaI 813 TCATGA T^CATGA
SapI 1210 GAAGAGC GCTCTTC(1/4)
SmaI 359 CCCGGG CCC^GGG
SmlI 914 CTCGAG C^T[CT][GA]AG
SphI 1164 GCATGC GCATG^C
SspI 48 AATATT AAT^ATT
Tth111I 220 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 473 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 671 CCAACAAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 914 CTCGAG C^TCGAG
XmaCI 359 CCCGGG C^CCGGG
XmaI 359 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 724 GATCC GGATC(4/5)
304 GGATC
AvaI 359 CCCGGG C^[CT]CG[GA]G
914 CTCGAG
BcnI 359 CCCGG CC^[GC]GG
360 CCGGG
BsmAI 218 GAGAC GTCTC(1/5)
1155 GAGAC
BsoBI 359 CCCGGG C^[CT]CG[GA]G
914 CTCGAG
BsrI 221 ACTGG ACTGG(1/-1)
1349 CCAGT
BstXI 17 CCAGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
93 CCAACAAGGTGG
CfoI 568 GCGC GCG^C
586 GCGC
Cfr13I 20 GGACC G^G[ACGT]CC
618 GGCCC
DpnII 305 GATC ^GATC
724 GATC
DraI 372 TTTAAA TTT^AAA
1389 TTTAAA
EcoT14I 896 CCTTGG C^C[AT][AT]GG
1380 CCTTGG
HhaI 568 GCGC GCG^C
586 GCGC
HinP1I 568 GCGC G^CGC
586 GCGC
MboI 305 GATC ^GATC
724 GATC
MslI 308 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
399 CACCTGTGTG
NciI 359 CCCGG CC^[GC]GG
360 CCGGG
NdeII 305 GATC ^GATC
724 GATC
SacI 817 GAGCTC GAGCT^C
1203 GAGCTC
Sau3AI 305 GATC ^GATC
724 GATC
Sau96I 20 GGACC G^G[ACGT]CC
618 GGCCC
SfaNI 82 GATGC GCATC(5/9)
574 GATGC
StyI 896 CCTTGG C^C[AT][AT]GG
1380 CCTTGG
TaqI 903 TCGA T^CGA
915 TCGA
TthHB8I 903 TCGA T^CGA
915 TCGA
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwNI AspHI BanII
BbvI BfaI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I DdeI
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboII MnlI MseI MvaI
MwoI NlaIII NlaIV PleI PstI
ScrFI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI