(Circular) (Six-base) MAPSORT of: pFN21AB9448.seq Check: 9125 from: 1 to: 6166 pFN21AB9448 6166 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3976 278 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3509 1937 Size: 1572 4594 Acc65I G'GTAC_C Cuts at: 3487 3487 Size: 6166 AclI AA'CG_TT Cuts at: 1563 4293 4666 1563 Size: 2730 373 3063 Fragments arranged by size: 3063 2730 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3474 4229 5613 5881 1632 Size: 1842 755 1384 268 1917 Fragments arranged by size: 1917 1842 1384 755 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5969 AflIII A'CryG_T Cuts at: 1203 5065 6042 1203 Size: 3862 977 1327 Fragments arranged by size: 3862 1327 977 AgeI A'CCGG_T Cuts at: 4997 4997 Size: 6166 AhdI GACnn_n'nnGTC Cuts at: 4895 4895 Size: 6166 AleI CACnn'nnGTG Cuts at: 1166 2408 1166 Size: 1242 4924 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6166 AlwNI CAG_nnn'CTG Cuts at: 2260 2484 3452 3909 5481 2260 Size: 224 968 457 1572 2945 Fragments arranged by size: 2945 1572 968 457 224 ApaLI G'TGCA_C Cuts at: 4223 5379 4223 Size: 1156 5010 ApoI r'AATT_y Cuts at: 1493 2748 3475 3701 3853 5962 1493 Size: 1255 727 226 152 2109 1697 Fragments arranged by size: 2109 1697 1255 727 226 152 AseI AT'TA_AT Cuts at: 160 1032 4720 160 Size: 872 3688 1606 Fragments arranged by size: 3688 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 AvaI C'yCGr_G Cuts at: 1870 1943 2364 2919 3491 1870 Size: 73 421 555 572 4545 Fragments arranged by size: 4545 572 555 421 73 BamHI G'GATC_C Cuts at: 3496 3863 3496 Size: 367 5799 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3487 4942 618 Size: 325 217 627 1700 1455 1842 Fragments arranged by size: 1842 1700 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2543 2826 3212 3485 1391 Size: 1152 283 386 273 4072 Fragments arranged by size: 4072 1152 386 283 273 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5195 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4357 4391 1903 Size: 34 2420 34 3678 Fragments arranged by size: 3678 2420 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4058 5268 4058 Size: 1210 4956 BclI T'GATC_A Cuts at: 1548 1548 Size: 6166 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4777 136 Size: 107 122 71 631 605 3105 1525 Fragments arranged by size: 3105 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6161 6161 Size: 6166 BlpI GC'TnA_GC Cuts at: 3338 3583 3338 Size: 245 5921 Bme1580I G_kGCm'C Cuts at: 1790 4227 5383 1790 Size: 2437 1156 2573 Fragments arranged by size: 2573 2437 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6166 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2236 3348 3897 Size: 416 53 83 399 24 819 1112 549 Cuts at: 3897 4855 442 Size: 958 1753 Fragments arranged by size: 1753 1112 958 819 549 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2184 1056 Size: 1128 5038 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4826 1239 Size: 3587 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4291 5156 5454 4291 Size: 865 298 5003 Fragments arranged by size: 5003 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4829 882 Size: 617 3330 2219 Fragments arranged by size: 3330 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3957 493 Size: 711 2753 2702 Fragments arranged by size: 2753 2702 711 BsaBI GATnn'nnATC Cuts at: 2727 6160 2727 Size: 3433 2733 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3973 4355 4990 275 Size: 53 83 186 936 2440 382 635 1451 Fragments arranged by size: 2440 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4599 4997 5271 5418 5829 4599 Size: 398 274 147 411 4936 Fragments arranged by size: 4936 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2507 2525 2537 2555 2999 Size: 30 165 30 1104 18 12 18 444 Cuts at: 2999 3029 1178 Size: 30 4315 Fragments arranged by size: 4315 1104 444 165 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2756 3193 1186 Size: 973 597 437 4159 Fragments arranged by size: 4159 973 597 437 BseYI C'CCAG_C Cuts at: 2323 2527 5369 5865 2323 Size: 204 2842 496 2624 Fragments arranged by size: 2842 2624 496 204 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2432 1163 Size: 573 696 4897 Fragments arranged by size: 4897 696 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3616 4377 5405 1540 Size: 275 186 1615 761 1028 2301 Fragments arranged by size: 2301 1615 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2517 2826 3212 3222 4227 4312 5383 2517 Size: 309 386 10 1005 85 1071 3300 Fragments arranged by size: 3300 1071 1005 386 309 85 10 BsmI GAATG_Cn' Cuts at: 3291 3677 3770 3291 Size: 386 93 5687 Fragments arranged by size: 5687 386 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2517 2543 2826 3212 3222 3485 4227 Size: 399 727 26 283 386 10 263 742 Cuts at: 4227 4312 5383 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 727 399 386 283 263 85 26 10 BspEI T'CCGG_A Cuts at: 5829 5829 Size: 6166 BspHI T'CATG_A Cuts at: 2818 4055 5785 2818 Size: 1237 1730 3199 Fragments arranged by size: 3199 1730 1237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3521 844 Size: 2677 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2617 4053 1394 Size: 429 794 1436 3507 Fragments arranged by size: 3507 1436 794 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2365 4661 4835 65 Size: 1183 1117 2296 174 1396 Fragments arranged by size: 2296 1396 1183 1117 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4810 4997 1700 Size: 110 3000 187 2869 Fragments arranged by size: 3000 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6166 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6166 BssSI C'ACGA_G Cuts at: 2168 4226 5238 2168 Size: 2058 1012 3096 Fragments arranged by size: 3096 2058 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6166 BstBI TT'CG_AA Cuts at: 4970 4970 Size: 6166 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6166 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2105 2029 Size: 76 6090 BstYI r'GATC_y Cuts at: 1972 3496 3863 4248 4265 5717 5803 5815 6161 Size: 1524 367 385 17 1452 86 12 346 Cuts at: 6161 1972 Size: 1977 Fragments arranged by size: 1977 1524 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4675 Fragments arranged by size: 4675 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5427 Fragments arranged by size: 5427 683 56 BtsI GCAGTG_nn' Cuts at: 2456 2572 2630 3682 4476 4496 2456 Size: 116 58 1052 794 20 4126 Fragments arranged by size: 4126 1052 794 116 58 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6166 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6131 DraI TTT'AAA Cuts at: 2379 3396 3470 2379 Size: 1017 74 5075 Fragments arranged by size: 5075 1017 74 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5173 809 Size: 507 3857 1802 Fragments arranged by size: 3857 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2688 3613 4502 Size: 54 1425 50 275 18 858 925 889 Cuts at: 4502 6131 8 Size: 1629 43 Fragments arranged by size: 1629 1425 925 889 858 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3613 1537 Size: 275 1801 4090 Fragments arranged by size: 4090 1801 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2878 3053 3210 4096 1353 Size: 783 742 175 157 886 3423 Fragments arranged by size: 3423 886 783 742 175 157 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4753 5127 5273 387 Size: 1064 138 3164 374 146 1280 Fragments arranged by size: 3164 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2824 3210 2824 Size: 386 5780 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3474 4229 4826 5613 5881 1239 Size: 393 1842 755 597 787 268 1524 Fragments arranged by size: 1842 1524 787 755 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2020 2020 Size: 6166 EcoO109I rG'GnC_Cy Cuts at: 2025 2025 Size: 6166 EcoRI G'AATT_C Cuts at: 3475 3853 3475 Size: 378 5788 FspI TGC'GCA Cuts at: 4672 4672 Size: 6166 HaeII r_GCGC'y Cuts at: 1678 2576 5313 1678 Size: 898 2737 2531 Fragments arranged by size: 2737 2531 898 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2214 2246 2525 2557 2969 Size: 32 135 32 642 32 279 32 412 Cuts at: 2969 3001 3350 3382 4807 4839 4881 4913 1373 Size: 32 349 32 1425 32 42 32 2626 Fragments arranged by size: 2626 1425 642 412 349 279 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3510 3756 669 Size: 988 281 1572 246 3079 Fragments arranged by size: 3079 1572 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6166 HpaI GTT'AAC Cuts at: 3756 3756 Size: 6166 KpnI G_GTAC'C Cuts at: 3491 3491 Size: 6166 MfeI C'AATT_G Cuts at: 3765 3765 Size: 6166 MluI A'CGCG_T Cuts at: 6042 6042 Size: 6166 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2122 5280 5464 1305 Size: 817 3158 184 2007 Fragments arranged by size: 3158 2007 817 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2690 10 Size: 54 1425 343 858 3486 Fragments arranged by size: 3486 1425 858 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2317 2408 4124 4483 4642 518 Size: 648 1151 91 1716 359 159 2042 Fragments arranged by size: 2042 1716 1151 648 359 159 91 MspA1I CmG'CkG Cuts at: 1606 2044 3582 4259 5407 5652 1606 Size: 438 1538 677 1148 245 2120 Fragments arranged by size: 2120 1538 1148 677 438 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4675 Fragments arranged by size: 4675 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6166 NheI G'CTAG_C Cuts at: 1052 2180 1052 Size: 1128 5038 NotI GC'GGCC_GC Cuts at: 3613 3613 Size: 6166 NspI r_CATG'y Cuts at: 1132 1229 3173 3524 5069 1132 Size: 97 1944 351 1545 2229 Fragments arranged by size: 2229 1944 1545 351 97 PasI CC'CwG_GG Cuts at: 1348 2029 2240 1348 Size: 681 211 5274 Fragments arranged by size: 5274 681 211 PciI A'CATG_T Cuts at: 5065 5065 Size: 6166 PflMI CCAn_nnn'nTGG Cuts at: 1108 2484 1108 Size: 1376 4790 PfoI T'CCnGG_A Cuts at: 3027 3027 Size: 6166 PmeI GTTT'AAAC Cuts at: 3470 3470 Size: 6166 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4235 4267 5748 5780 4235 Size: 32 1481 32 4621 Fragments arranged by size: 4621 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2025 2025 Size: 6166 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6166 PsiI TTA'TAA Cuts at: 3736 3736 Size: 6166 PstI C_TGCA'G Cuts at: 830 2211 2339 2463 3168 3193 3518 830 Size: 1381 128 124 705 25 325 3478 Fragments arranged by size: 3478 1381 705 325 128 124 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6166 PvuII CAG'CTG Cuts at: 2044 2044 Size: 6166 SacI G_AGCT'C Cuts at: 2826 3212 2826 Size: 386 5780 SalI G'TCGA_C Cuts at: 1936 3508 1936 Size: 1572 4594 SapI GCTCTTCn'nnn_ Cuts at: 3210 3210 Size: 6166 SbfI CC_TGCA'GG Cuts at: 3518 3518 Size: 6166 ScaI AGT'ACT Cuts at: 4414 6126 4414 Size: 1712 4454 SfcI C'TryA_G Cuts at: 826 1045 2207 2335 2459 3164 3189 3514 4649 Size: 219 1162 128 124 705 25 325 1135 Cuts at: 4649 5330 5521 826 Size: 681 191 1471 Fragments arranged by size: 1471 1162 1135 705 681 325 219 191 128 124 25 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6166 SmaI CCC'GGG Cuts at: 1872 2366 3493 1872 Size: 494 1127 4545 Fragments arranged by size: 4545 1127 494 SmlI C'TyrA_G Cuts at: 820 1017 1943 2919 4270 5171 5433 820 Size: 197 926 976 1351 901 262 1553 Fragments arranged by size: 1553 1351 976 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3957 493 Size: 3464 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6166 SphI G_CATG'C Cuts at: 1132 3173 3524 1132 Size: 2041 351 3774 Fragments arranged by size: 3774 2041 351 SspI AAT'ATT Cuts at: 5 52 2055 4090 5 Size: 47 2003 2035 2081 Fragments arranged by size: 2081 2035 2003 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6166 StyI C'CwwG_G Cuts at: 513 1065 2004 2901 3385 3605 513 Size: 552 939 897 484 220 3074 Fragments arranged by size: 3074 939 897 552 484 220 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4193 4210 4363 4548 4993 325 Size: 1255 2613 17 153 185 445 1498 Fragments arranged by size: 2613 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4412 6124 96 Size: 274 80 33 51 1443 2435 1712 138 Fragments arranged by size: 2435 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2507 2539 2746 2778 6126 6158 2507 Size: 32 207 32 3348 32 2515 Fragments arranged by size: 3348 2515 207 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2228 1316 Size: 912 5254 XbaI T'CTAG_A Cuts at: 3502 3502 Size: 6166 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2683 2683 Size: 6166 XhoI C'TCGA_G Cuts at: 1943 2919 1943 Size: 976 5190 XmaI C'CCGG_G Cuts at: 1870 2364 3491 1870 Size: 494 1127 4545 Fragments arranged by size: 4545 1127 494 XmnI GAAnn'nnTTC Cuts at: 4295 4295 Size: 6166 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3974 276 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI