(Circular) (Six-base) MAPSORT of: pFN21AB9446.seq Check: 5053 from: 1 to: 5674 pFN21AB9446 5674 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3017 1937 Size: 1080 4594 Acc65I G'GTAC_C Cuts at: 2693 2995 2693 Size: 302 5372 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2508 3737 5121 5389 1632 Size: 876 1229 1384 268 1917 Fragments arranged by size: 1917 1384 1229 876 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5477 AflIII A'CryG_T Cuts at: 1203 4573 5550 1203 Size: 3370 977 1327 Fragments arranged by size: 3370 1327 977 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 2301 4403 2301 Size: 2102 3572 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5674 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5674 AlwNI CAG_nnn'CTG Cuts at: 3417 4989 3417 Size: 1572 4102 ApaI G_GGCC'C Cuts at: 2419 2419 Size: 5674 ApaLI G'TGCA_C Cuts at: 3731 4887 3731 Size: 1156 4518 ApoI r'AATT_y Cuts at: 1493 2033 2711 2736 2905 2983 3209 3361 5470 Size: 540 678 25 169 78 226 152 2109 Cuts at: 5470 1493 Size: 1697 Fragments arranged by size: 2109 1697 678 540 226 169 152 78 25 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2418 2999 1870 Size: 73 475 581 4545 Fragments arranged by size: 4545 581 475 73 BamHI G'GATC_C Cuts at: 3004 3371 3004 Size: 367 5307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2693 2995 4450 618 Size: 325 217 627 221 685 302 1455 1842 Fragments arranged by size: 1842 1455 685 627 325 302 221 217 BanII G_rGCy'C Cuts at: 1391 2419 2993 1391 Size: 1028 574 4072 Fragments arranged by size: 4072 1028 574 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 5674 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2300 928 Size: 971 401 4302 Fragments arranged by size: 4302 971 401 BbvCI CC'TCA_GC Cuts at: 2394 2394 Size: 5674 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2040 2074 3865 3899 1903 Size: 34 103 34 1791 34 3678 Fragments arranged by size: 3678 1791 103 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 1548 Size: 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4285 136 Size: 107 122 71 631 605 2613 1525 Fragments arranged by size: 2613 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5669 5669 Size: 5674 BlpI GC'TnA_GC Cuts at: 3091 3091 Size: 5674 Bme1580I G_kGCm'C Cuts at: 1790 2419 3735 4891 1790 Size: 629 1316 1156 2573 Fragments arranged by size: 2573 1316 1156 629 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5674 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2527 3405 4363 Size: 416 53 83 399 24 1110 878 958 Cuts at: 4363 442 Size: 1753 Fragments arranged by size: 1753 1110 958 878 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4334 1239 Size: 3095 2579 Bpu10I CC'TnA_GC Cuts at: 2394 2394 Size: 5674 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2606 3799 4664 4962 2606 Size: 1193 865 298 3318 Fragments arranged by size: 3318 1193 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2381 2713 4337 882 Size: 617 882 332 1624 2219 Fragments arranged by size: 2219 1624 882 617 332 BsaAI yAC'GTr Cuts at: 493 1204 2200 3465 493 Size: 711 996 1265 2702 Fragments arranged by size: 2702 1265 996 711 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3481 3863 4498 Size: 53 83 186 936 476 1472 382 635 Cuts at: 4498 275 Size: 1451 Fragments arranged by size: 1472 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2176 4107 4505 4779 4926 5337 2176 Size: 1931 398 274 147 411 2513 Fragments arranged by size: 2513 1931 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5449 Fragments arranged by size: 5449 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2391 2937 1186 Size: 1205 546 3923 Fragments arranged by size: 3923 1205 546 BseYI C'CCAG_C Cuts at: 2697 4877 5373 2697 Size: 2180 496 2998 Fragments arranged by size: 2998 2180 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3124 3885 4913 1540 Size: 275 186 1123 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3735 3820 4891 3735 Size: 85 1071 4518 Fragments arranged by size: 4518 1071 85 BsmI GAATG_Cn' Cuts at: 3185 3278 3185 Size: 93 5581 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2419 2993 3735 3820 4891 1391 Size: 399 629 574 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 629 574 399 85 BspEI T'CCGG_A Cuts at: 2176 5337 2176 Size: 3161 2513 BspHI T'CATG_A Cuts at: 2454 3563 5293 2454 Size: 1109 1730 2835 Fragments arranged by size: 2835 1730 1109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3029 844 Size: 2185 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3561 1394 Size: 429 1738 3507 Fragments arranged by size: 3507 1738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2826 4169 4343 65 Size: 1183 1578 1343 174 1396 Fragments arranged by size: 1578 1396 1343 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4318 4505 1700 Size: 110 2508 187 2869 Fragments arranged by size: 2869 2508 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5674 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5674 BssSI C'ACGA_G Cuts at: 2613 3734 4746 2613 Size: 1121 1012 3541 Fragments arranged by size: 3541 1121 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5674 BstBI TT'CG_AA Cuts at: 4478 4478 Size: 5674 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5674 BstXI CCAn_nnnn'nTGG Cuts at: 2777 2777 Size: 5674 BstYI r'GATC_y Cuts at: 1972 2425 3004 3371 3756 3773 5225 5311 5323 Size: 453 579 367 385 17 1452 86 12 Cuts at: 5323 5669 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 579 453 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2501 513 Size: 552 939 497 3686 Fragments arranged by size: 3686 939 552 497 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4935 Fragments arranged by size: 4935 683 56 BtsI GCAGTG_nn' Cuts at: 3190 3984 4004 3190 Size: 794 20 4860 Fragments arranged by size: 4860 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3121 4010 5639 Size: 54 1425 50 275 18 1291 889 1629 Cuts at: 5639 8 Size: 43 Fragments arranged by size: 1629 1425 1291 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 1537 Size: 275 1309 4090 Fragments arranged by size: 4090 1309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2079 2484 2843 3604 1353 Size: 726 405 359 761 3423 Fragments arranged by size: 3423 761 726 405 359 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2508 3737 4334 5121 5389 1239 Size: 393 876 1229 597 787 268 1524 Fragments arranged by size: 1524 1229 876 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2020 2415 2020 Size: 395 5279 EcoRI G'AATT_C Cuts at: 2905 2983 3361 2905 Size: 78 378 5218 Fragments arranged by size: 5218 378 78 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 2012 2786 4821 1678 Size: 334 774 2035 2531 Fragments arranged by size: 2531 2035 774 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2287 2319 4315 4347 4389 Size: 32 135 32 715 32 1996 32 42 Cuts at: 4389 4421 1373 Size: 32 2626 Fragments arranged by size: 2626 1996 715 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3018 3264 669 Size: 988 281 1080 246 3079 Fragments arranged by size: 3079 1080 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5674 HpaI GTT'AAC Cuts at: 3264 3264 Size: 5674 KasI G'GCGC_C Cuts at: 2008 2008 Size: 5674 KpnI G_GTAC'C Cuts at: 2697 2999 2697 Size: 302 5372 MfeI C'AATT_G Cuts at: 3273 3273 Size: 5674 MluI A'CGCG_T Cuts at: 5550 5550 Size: 5674 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2129 4788 4972 1305 Size: 824 2659 184 2007 Fragments arranged by size: 2659 2007 824 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3852 Fragments arranged by size: 3852 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2142 2754 3632 3991 4150 518 Size: 648 976 612 878 359 159 2042 Fragments arranged by size: 2042 976 878 648 612 359 159 MspA1I CmG'CkG Cuts at: 1606 3090 3767 4915 5160 1606 Size: 1484 677 1148 245 2120 Fragments arranged by size: 2120 1484 1148 677 245 NarI GG'CG_CC Cuts at: 2009 2009 Size: 5674 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4183 Fragments arranged by size: 4183 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5674 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5674 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 3032 4577 1132 Size: 97 1803 1545 2229 Fragments arranged by size: 2229 1803 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5674 PciI A'CATG_T Cuts at: 4573 4573 Size: 5674 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5674 PfoI T'CCnGG_A Cuts at: 2037 2037 Size: 5674 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2020 2020 Size: 5674 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5674 PsiI TTA'TAA Cuts at: 3244 3244 Size: 5674 PspOMI G'GGCC_C Cuts at: 2415 2415 Size: 5674 PstI C_TGCA'G Cuts at: 830 3026 830 Size: 2196 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SapI GCTCTTCn'nnn_ Cuts at: 2484 2843 2484 Size: 359 5315 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 2190 3922 5634 2190 Size: 1732 1712 2230 Fragments arranged by size: 2230 1732 1712 SfcI C'TryA_G Cuts at: 826 1045 3022 4157 4838 5029 826 Size: 219 1977 1135 681 191 1471 Fragments arranged by size: 1977 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2010 2010 Size: 5674 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 2420 3001 1872 Size: 548 581 4545 Fragments arranged by size: 4545 581 548 SmlI C'TyrA_G Cuts at: 820 1017 1943 2585 3778 4679 4941 820 Size: 197 926 642 1193 901 262 1553 Fragments arranged by size: 1553 1193 926 901 642 262 197 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SspI AAT'ATT Cuts at: 5 52 3598 5 Size: 47 3546 2081 Fragments arranged by size: 3546 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5674 StyI C'CwwG_G Cuts at: 513 1065 2004 3113 513 Size: 552 939 1109 3074 Fragments arranged by size: 3074 1109 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2776 3701 3718 3871 4056 4501 325 Size: 1255 1196 925 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1196 925 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2188 3920 5632 Size: 274 80 33 51 1443 211 1732 1712 Cuts at: 5632 96 Size: 138 Fragments arranged by size: 1732 1712 1443 274 211 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5634 5666 5634 Size: 32 5642 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5674 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5674 XmaI C'CCGG_G Cuts at: 1870 2418 2999 1870 Size: 548 581 4545 Fragments arranged by size: 4545 581 548 XmnI GAAnn'nnTTC Cuts at: 2129 2172 3803 2129 Size: 43 1631 4000 Fragments arranged by size: 4000 1631 43 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI