(Circular) (Six-base) MAPSORT of: pFN21AB9430.seq Check: 830 from: 1 to: 6004 pFN21AB9430 6004 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2892 3347 1937 Size: 955 455 4594 Fragments arranged by size: 4594 955 455 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3130 4067 5451 5719 1632 Size: 1498 937 1384 268 1917 Fragments arranged by size: 1917 1498 1384 937 268 AfeI AGC'GCT Cuts at: 2043 2844 2043 Size: 801 5203 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 3012 4903 5880 1203 Size: 1809 1891 977 1327 Fragments arranged by size: 1891 1809 1327 977 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AlwNI CAG_nnn'CTG Cuts at: 2696 3747 5319 2696 Size: 1051 1572 3381 Fragments arranged by size: 3381 1572 1051 ApaLI G'TGCA_C Cuts at: 4061 5217 4061 Size: 1156 4848 ApoI r'AATT_y Cuts at: 1493 3313 3539 3691 5800 1493 Size: 1820 226 152 2109 1697 Fragments arranged by size: 2109 1820 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2081 2149 2167 2854 3055 3236 Size: 73 125 13 68 18 687 201 181 Cuts at: 3236 3256 3269 3329 1870 Size: 20 13 60 4545 Fragments arranged by size: 4545 687 201 181 125 73 68 60 20 18 13 13 BamHI G'GATC_C Cuts at: 3334 3701 3334 Size: 367 5637 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2567 2756 3325 4780 Size: 325 217 627 285 495 189 569 1455 Cuts at: 4780 618 Size: 1842 Fragments arranged by size: 1842 1455 627 569 495 325 285 217 189 BanII G_rGCy'C Cuts at: 1391 2063 3062 3195 3323 1391 Size: 672 999 133 128 4072 Fragments arranged by size: 4072 999 672 133 128 BbeI G_GCGC'C Cuts at: 2076 2571 2760 2076 Size: 495 189 5320 Fragments arranged by size: 5320 495 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3093 928 Size: 971 1194 3839 Fragments arranged by size: 3839 1194 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4195 4229 1903 Size: 34 2258 34 3678 Fragments arranged by size: 3678 2258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 1548 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2505 2726 2822 Size: 107 122 71 631 605 833 221 96 Cuts at: 2822 4615 136 Size: 1793 1525 Fragments arranged by size: 1793 1525 833 631 605 221 122 107 96 71 BglII A'GATC_T Cuts at: 2268 5999 2268 Size: 3731 2273 BlpI GC'TnA_GC Cuts at: 3421 3421 Size: 6004 Bme1580I G_kGCm'C Cuts at: 1790 2178 2915 4065 5221 1790 Size: 388 737 1150 1156 2573 Fragments arranged by size: 2573 1156 1150 737 388 BmgBI CAC'GTC Cuts at: 1318 2916 1318 Size: 1598 4406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3735 4693 442 Size: 416 53 83 399 24 2318 958 1753 Fragments arranged by size: 2318 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3214 3271 3284 3214 Size: 57 13 5934 Fragments arranged by size: 5934 57 13 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2410 4664 1239 Size: 1171 2254 2579 Fragments arranged by size: 2579 2254 1171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2339 4129 4994 5292 2339 Size: 1790 865 298 3051 Fragments arranged by size: 3051 1790 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4667 882 Size: 617 3168 2219 Fragments arranged by size: 3168 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3795 493 Size: 711 2591 2702 Fragments arranged by size: 2702 2591 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 2568 2757 3811 Size: 53 83 186 936 540 495 189 1054 Cuts at: 3811 4193 4828 275 Size: 382 635 1451 Fragments arranged by size: 1451 1054 936 635 540 495 382 189 186 83 53 BsaWI w'CCGG_w Cuts at: 4437 4835 5109 5256 5667 4437 Size: 398 274 147 411 4774 Fragments arranged by size: 4774 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2408 2414 2438 2444 2460 Size: 30 165 30 1005 6 24 6 16 Cuts at: 2460 2490 3002 3032 1178 Size: 30 512 30 4150 Fragments arranged by size: 4150 1005 512 165 30 30 30 30 24 16 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2089 2422 2437 2556 2809 3010 3028 3031 Size: 903 333 15 119 253 201 18 3 Cuts at: 3031 3040 3043 3052 1186 Size: 9 3 9 4138 Fragments arranged by size: 4138 903 333 253 201 119 18 15 9 9 3 3 BseYI C'CCAG_C Cuts at: 2086 2281 2798 3110 5207 5703 2086 Size: 195 517 312 2097 496 2387 Fragments arranged by size: 2387 2097 517 496 312 195 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2030 3214 1163 Size: 573 294 1184 3953 Fragments arranged by size: 3953 1184 573 294 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2439 2938 3169 3454 4215 5243 Size: 275 186 438 499 231 285 761 1028 Cuts at: 5243 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 499 438 285 275 231 186 BsiHKAI G_wGCw'C Cuts at: 2063 4065 4150 5221 2063 Size: 2002 85 1071 2846 Fragments arranged by size: 2846 2002 1071 85 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2178 2915 3062 3195 3323 4065 Size: 399 273 115 737 147 133 128 742 Cuts at: 4065 4150 5221 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 737 399 273 147 133 128 115 85 BspEI T'CCGG_A Cuts at: 5667 5667 Size: 6004 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3102 3359 844 Size: 2258 257 3489 Fragments arranged by size: 3489 2258 257 BsrBI CCG'CTC Cuts at: 1394 1823 2224 2344 2940 3891 1394 Size: 429 401 120 596 951 3507 Fragments arranged by size: 3507 951 596 429 401 120 BsrDI GCAATG_nn' Cuts at: 65 1248 4499 4673 65 Size: 1183 3251 174 1396 Fragments arranged by size: 3251 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 2075 2388 2581 2933 4648 4835 Size: 110 241 24 313 193 352 1715 187 Cuts at: 4835 1700 Size: 2869 Fragments arranged by size: 2869 1715 352 313 241 193 187 110 24 BsrGI T'GTAC_A Cuts at: 96 2781 96 Size: 2685 3319 BssHII G'CGCG_C Cuts at: 1925 2974 3000 1925 Size: 1049 26 4929 Fragments arranged by size: 4929 1049 26 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 2696 794 Size: 1902 4102 BstBI TT'CG_AA Cuts at: 3084 4808 3084 Size: 1724 4280 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstXI CCAn_nnnn'nTGG Cuts at: 2281 2281 Size: 6004 BstYI r'GATC_y Cuts at: 1972 2268 2319 3334 3701 4086 4103 5555 5641 Size: 296 51 1015 367 385 17 1452 86 Cuts at: 5641 5653 5999 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1015 385 367 346 296 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2127 2500 2956 513 Size: 552 939 123 373 456 3561 Fragments arranged by size: 3561 939 552 456 373 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 2491 3520 4314 4334 2491 Size: 1029 794 20 4161 Fragments arranged by size: 4161 1029 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2461 2496 529 Size: 35 1897 35 4037 Fragments arranged by size: 4037 1897 35 35 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2436 2497 Size: 54 1425 50 275 18 219 387 61 Cuts at: 2497 2935 3451 4340 5969 8 Size: 438 516 889 1629 43 Fragments arranged by size: 1629 1425 889 516 438 387 275 219 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2436 2935 3451 1537 Size: 275 624 499 516 4090 Fragments arranged by size: 4090 624 516 499 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3049 3934 1353 Size: 1696 885 3423 Fragments arranged by size: 3423 1696 885 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2354 2803 2885 4591 4965 5111 Size: 1064 138 765 449 82 1706 374 146 Cuts at: 5111 387 Size: 1280 Fragments arranged by size: 1706 1280 1064 765 449 374 146 138 82 EcoICRI GAG'CTC Cuts at: 2061 2061 Size: 6004 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2410 3130 4067 4664 5451 5719 1239 Size: 393 778 720 937 597 787 268 1524 Fragments arranged by size: 1524 937 787 778 720 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3172 3172 Size: 6004 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 2045 2076 2571 2760 2805 2846 3164 5151 Size: 367 31 495 189 45 41 318 1987 Cuts at: 5151 1678 Size: 2531 Fragments arranged by size: 2531 1987 495 367 318 189 45 41 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2460 2492 2759 2791 3116 Size: 32 135 32 888 32 267 32 325 Cuts at: 3116 3148 4645 4677 4719 4751 1373 Size: 32 1497 32 42 32 2626 Fragments arranged by size: 2626 1497 888 325 267 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KasI G'GCGC_C Cuts at: 2072 2567 2756 2072 Size: 495 189 5320 Fragments arranged by size: 5320 495 189 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 3012 5880 3012 Size: 2868 3136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3096 3309 5118 5302 1305 Size: 1791 213 1809 184 2007 Fragments arranged by size: 2007 1809 1791 213 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4182 Fragments arranged by size: 4182 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3962 4321 4480 518 Size: 648 2796 359 159 2042 Fragments arranged by size: 2796 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2693 2958 3420 4097 5245 5490 1606 Size: 1087 265 462 677 1148 245 2120 Fragments arranged by size: 2120 1148 1087 677 462 265 245 NaeI GCC'GGC Cuts at: 2053 2077 2935 2053 Size: 24 858 5122 Fragments arranged by size: 5122 858 24 NarI GG'CG_CC Cuts at: 2073 2568 2757 2073 Size: 495 189 5320 Fragments arranged by size: 5320 495 189 NcoI C'CATG_G Cuts at: 513 1065 2004 2500 513 Size: 552 939 496 4017 Fragments arranged by size: 4017 939 552 496 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NgoMIV G'CCGG_C Cuts at: 2051 2075 2933 2051 Size: 24 858 5122 Fragments arranged by size: 5122 858 24 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 2257 3133 3362 4907 1132 Size: 97 1028 876 229 1545 2229 Fragments arranged by size: 2229 1545 1028 876 229 97 PasI CC'CwG_GG Cuts at: 1348 2752 3136 1348 Size: 1404 384 4216 Fragments arranged by size: 4216 1404 384 PciI A'CATG_T Cuts at: 4903 4903 Size: 6004 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6004 PfoI T'CCnGG_A Cuts at: 3219 3219 Size: 6004 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3098 3130 4073 4105 5586 5618 3098 Size: 32 943 32 1481 32 3484 Fragments arranged by size: 3484 1481 943 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3172 3172 Size: 6004 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PspXI vC'TCGA_Gb Cuts at: 2081 3269 2081 Size: 1188 4816 PstI C_TGCA'G Cuts at: 830 3356 830 Size: 2526 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 SacI G_AGCT'C Cuts at: 2063 2063 Size: 6004 SacII CC_GC'GG Cuts at: 2959 2959 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 4252 5964 4252 Size: 1712 4292 SexAI A'CCwGG_T Cuts at: 3288 3288 Size: 6004 SfcI C'TryA_G Cuts at: 826 1045 3352 4487 5168 5359 826 Size: 219 2307 1135 681 191 1471 Fragments arranged by size: 2307 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2074 2569 2758 2074 Size: 495 189 5320 Fragments arranged by size: 5320 495 189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 2856 3331 1872 Size: 984 475 4545 Fragments arranged by size: 4545 984 475 SmlI C'TyrA_G Cuts at: 820 1017 1943 2081 2354 3269 4108 5009 5271 Size: 197 926 138 273 915 839 901 262 Cuts at: 5271 820 Size: 1553 Fragments arranged by size: 1553 926 915 901 839 273 262 197 138 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6004 StyI C'CwwG_G Cuts at: 513 1065 2004 2500 3443 513 Size: 552 939 496 943 3074 Fragments arranged by size: 3074 943 939 552 496 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4031 4048 4201 4386 4831 325 Size: 1255 2451 17 153 185 445 1498 Fragments arranged by size: 2451 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2781 4250 5962 Size: 274 80 33 51 1443 804 1469 1712 Cuts at: 5962 96 Size: 138 Fragments arranged by size: 1712 1469 1443 804 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3002 3034 5964 5996 3002 Size: 32 2930 32 3010 Fragments arranged by size: 3010 2930 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2474 1316 Size: 1158 4846 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2659 2659 Size: 6004 XhoI C'TCGA_G Cuts at: 1943 2081 3269 1943 Size: 138 1188 4678 Fragments arranged by size: 4678 1188 138 XmaI C'CCGG_G Cuts at: 1870 2854 3329 1870 Size: 984 475 4545 Fragments arranged by size: 4545 984 475 XmnI GAAnn'nnTTC Cuts at: 2198 4133 2198 Size: 1935 4069 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SanDI SapI SfiI SrfI SwaI