(Circular) (Six-base) MAPSORT of: pFN21AB9429.seq Check: 6264 from: 1 to: 6385 pFN21AB9429 6385 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4195 278 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3087 3728 1937 Size: 1150 641 4594 Fragments arranged by size: 4594 1150 641 Acc65I G'GTAC_C Cuts at: 3706 3706 Size: 6385 AclI AA'CG_TT Cuts at: 1563 4512 4885 1563 Size: 2949 373 3063 Fragments arranged by size: 3063 2949 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2097 2434 2864 3089 3374 4448 5832 6100 Size: 465 337 430 225 285 1074 1384 268 Cuts at: 6100 1632 Size: 1917 Fragments arranged by size: 1917 1384 1074 465 430 337 285 268 225 AfeI AGC'GCT Cuts at: 3068 3245 3068 Size: 177 6208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6188 AflIII A'CryG_T Cuts at: 1203 5284 6261 1203 Size: 4081 977 1327 Fragments arranged by size: 4081 1327 977 AgeI A'CCGG_T Cuts at: 5216 5216 Size: 6385 AhdI GACnn_n'nnGTC Cuts at: 5114 5114 Size: 6385 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6385 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3495 1184 Size: 2311 4074 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3306 3338 3306 Size: 32 6353 AlwNI CAG_nnn'CTG Cuts at: 2023 2272 4128 5700 2023 Size: 249 1856 1572 2708 Fragments arranged by size: 2708 1856 1572 249 ApaLI G'TGCA_C Cuts at: 4442 5598 4442 Size: 1156 5229 ApoI r'AATT_y Cuts at: 1493 2480 2813 3694 3920 4072 6181 1493 Size: 987 333 881 226 152 2109 1697 Fragments arranged by size: 2109 1697 987 881 333 226 152 AseI AT'TA_AT Cuts at: 160 1032 4939 160 Size: 872 3907 1606 Fragments arranged by size: 3907 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 AvaI C'yCGr_G Cuts at: 1870 1943 2087 3710 1870 Size: 73 144 1623 4545 Fragments arranged by size: 4545 1623 144 73 BamHI G'GATC_C Cuts at: 3442 3715 4082 3442 Size: 273 367 5745 Fragments arranged by size: 5745 367 273 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3161 3586 3706 5161 618 Size: 325 217 627 1374 425 120 1455 1842 Fragments arranged by size: 1842 1455 1374 627 425 325 217 120 BanII G_rGCy'C Cuts at: 1391 3704 1391 Size: 2313 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2259 2532 2571 2595 2870 3434 928 Size: 971 360 273 39 24 275 564 3879 Fragments arranged by size: 3879 971 564 360 275 273 39 24 BbvCI CC'TCA_GC Cuts at: 2379 2379 Size: 6385 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2354 2388 4576 4610 1903 Size: 34 417 34 2188 34 3678 Fragments arranged by size: 3678 2188 417 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4277 5487 4277 Size: 1210 5175 BclI T'GATC_A Cuts at: 1548 2398 1548 Size: 850 5535 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4996 136 Size: 107 122 71 631 605 3324 1525 Fragments arranged by size: 3324 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6380 6380 Size: 6385 BlpI GC'TnA_GC Cuts at: 3285 3802 3285 Size: 517 5868 Bme1580I G_kGCm'C Cuts at: 1790 2827 4446 5602 1790 Size: 1037 1619 1156 2573 Fragments arranged by size: 2573 1619 1156 1037 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6385 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2348 3155 4116 Size: 416 53 83 399 24 931 807 961 Cuts at: 4116 5074 442 Size: 958 1753 Fragments arranged by size: 1753 961 958 931 807 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6385 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2337 2366 2619 3053 5045 1239 Size: 1098 29 253 434 1992 2579 Fragments arranged by size: 2579 1992 1098 434 253 29 Bpu10I CC'TnA_GC Cuts at: 2379 3273 3563 2379 Size: 894 290 5201 Fragments arranged by size: 5201 894 290 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3315 4510 5375 5673 3315 Size: 1195 865 298 4027 Fragments arranged by size: 4027 1195 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5048 882 Size: 617 3549 2219 Fragments arranged by size: 3549 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4176 493 Size: 711 2972 2702 Fragments arranged by size: 2972 2702 711 BsaBI GATnn'nnATC Cuts at: 6379 6379 Size: 6385 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4192 4574 5209 275 Size: 53 83 186 936 2659 382 635 1451 Fragments arranged by size: 2659 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3316 4818 5216 5490 5637 6048 3316 Size: 1502 398 274 147 411 3653 Fragments arranged by size: 3653 1502 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6160 Fragments arranged by size: 6160 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2531 2534 2969 1186 Size: 1345 3 435 4602 Fragments arranged by size: 4602 1345 435 3 BseYI C'CCAG_C Cuts at: 3050 5588 6084 3050 Size: 2538 496 3351 Fragments arranged by size: 3351 2538 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5812 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3835 4596 5624 1540 Size: 275 186 1834 761 1028 2301 Fragments arranged by size: 2301 1834 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4446 4531 5602 4446 Size: 85 1071 5229 Fragments arranged by size: 5229 1071 85 BsmI GAATG_Cn' Cuts at: 3896 3989 3896 Size: 93 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2827 3704 4446 4531 5602 1391 Size: 399 1037 877 742 85 1071 2174 Fragments arranged by size: 2174 1071 1037 877 742 399 85 BspEI T'CCGG_A Cuts at: 6048 6048 Size: 6385 BspHI T'CATG_A Cuts at: 4274 6004 4274 Size: 1730 4655 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3740 844 Size: 2896 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3639 4272 1394 Size: 429 1816 633 3507 Fragments arranged by size: 3507 1816 633 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4880 5054 65 Size: 1183 3632 174 1396 Fragments arranged by size: 3632 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5029 5216 1700 Size: 110 3219 187 2869 Fragments arranged by size: 3219 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6385 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6385 BssSI C'ACGA_G Cuts at: 4445 5457 4445 Size: 1012 5373 BstAPI GCAn_nnn'nTGC Cuts at: 794 2272 794 Size: 1478 4907 BstBI TT'CG_AA Cuts at: 3226 5189 3226 Size: 1963 4422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6385 BstXI CCAn_nnnn'nTGG Cuts at: 2494 3058 3439 2494 Size: 564 381 5440 Fragments arranged by size: 5440 564 381 BstYI r'GATC_y Cuts at: 1972 3321 3442 3715 4082 4467 4484 5936 6022 Size: 1349 121 273 367 385 17 1452 86 Cuts at: 6022 6034 6380 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1349 385 367 346 273 121 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3462 3676 513 Size: 552 939 1458 214 3222 Fragments arranged by size: 3222 1458 939 552 214 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5646 Fragments arranged by size: 5646 683 56 BtsI GCAGTG_nn' Cuts at: 2097 2518 3479 3901 4695 4715 2097 Size: 421 961 422 794 20 3767 Fragments arranged by size: 3767 961 794 422 421 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6385 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6350 DraI TTT'AAA Cuts at: 3689 3689 Size: 6385 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3083 5392 809 Size: 507 1767 2309 1802 Fragments arranged by size: 2309 1802 1767 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3459 3649 3832 Size: 54 1425 50 275 18 1629 190 183 Cuts at: 3832 4721 6350 8 Size: 889 1629 43 Fragments arranged by size: 1629 1629 1425 889 275 190 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3832 1537 Size: 275 2020 4090 Fragments arranged by size: 4090 2020 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2411 2526 2671 2880 2982 4315 1353 Size: 1058 115 145 209 102 1333 3423 Fragments arranged by size: 3423 1333 1058 209 145 115 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2084 4972 5346 5492 387 Size: 1064 138 495 2888 374 146 1280 Fragments arranged by size: 2888 1280 1064 495 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2337 2366 2434 2619 2864 3053 Size: 393 465 240 29 68 185 245 189 Cuts at: 3053 3089 3374 4448 5045 5832 6100 1239 Size: 36 285 1074 597 787 268 1524 Fragments arranged by size: 1524 1074 787 597 465 393 285 268 245 240 189 185 68 36 29 EcoO109I rG'GnC_Cy Cuts at: 3013 3013 Size: 6385 EcoRI G'AATT_C Cuts at: 3694 4072 3694 Size: 378 6007 EcoRV GAT'ATC Cuts at: 2918 2918 Size: 6385 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2909 2909 Size: 6385 FspI TGC'GCA Cuts at: 4891 4891 Size: 6385 HaeII r_GCGC'y Cuts at: 1678 2030 3070 3247 5532 1678 Size: 352 1040 177 2285 2531 Fragments arranged by size: 2531 2285 1040 352 177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2282 2297 2314 2329 3313 Size: 32 135 32 710 15 17 15 984 Cuts at: 3313 3345 5026 5058 5100 5132 1373 Size: 32 1681 32 42 32 2626 Fragments arranged by size: 2626 1681 984 710 135 42 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2751 3729 3975 669 Size: 988 281 813 978 246 3079 Fragments arranged by size: 3079 988 978 813 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6385 HpaI GTT'AAC Cuts at: 3975 3975 Size: 6385 KpnI G_GTAC'C Cuts at: 3710 3710 Size: 6385 MfeI C'AATT_G Cuts at: 3984 3984 Size: 6385 MluI A'CGCG_T Cuts at: 6261 6261 Size: 6385 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5499 5683 1305 Size: 4194 184 2007 Fragments arranged by size: 4194 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3461 3651 10 Size: 54 1425 343 1629 190 2744 Fragments arranged by size: 2744 1629 1425 343 190 54 MslI CAynn'nnrTG Cuts at: 518 1166 3681 4343 4702 4861 518 Size: 648 2515 662 359 159 2042 Fragments arranged by size: 2515 2042 662 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3801 4478 5626 5871 1606 Size: 2195 677 1148 245 2120 Fragments arranged by size: 2195 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3462 513 Size: 552 939 1458 3436 Fragments arranged by size: 3436 1458 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6385 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6385 NotI GC'GGCC_GC Cuts at: 3832 3832 Size: 6385 NspI r_CATG'y Cuts at: 1132 1229 3743 5288 1132 Size: 97 2514 1545 2229 Fragments arranged by size: 2514 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6385 PciI A'CATG_T Cuts at: 5284 5284 Size: 6385 PflMI CCAn_nnn'nTGG Cuts at: 1108 2716 1108 Size: 1608 4777 PmeI GTTT'AAAC Cuts at: 3689 3689 Size: 6385 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4454 4486 5967 5999 4454 Size: 32 1481 32 4840 Fragments arranged by size: 4840 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3013 3013 Size: 6385 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6385 PsiI TTA'TAA Cuts at: 3955 3955 Size: 6385 PstI C_TGCA'G Cuts at: 830 3737 830 Size: 2907 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6385 SalI G'TCGA_C Cuts at: 1936 3727 1936 Size: 1791 4594 SanDI GG'GwC_CC Cuts at: 3013 3013 Size: 6385 SapI GCTCTTCn'nnn_ Cuts at: 2411 2671 2411 Size: 260 6125 SbfI CC_TGCA'GG Cuts at: 3737 3737 Size: 6385 ScaI AGT'ACT Cuts at: 4633 6345 4633 Size: 1712 4673 SfcI C'TryA_G Cuts at: 826 1045 3088 3733 4868 5549 5740 826 Size: 219 2043 645 1135 681 191 1471 Fragments arranged by size: 2043 1471 1135 681 645 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6385 SmaI CCC'GGG Cuts at: 1872 3712 1872 Size: 1840 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3330 4489 5390 5652 820 Size: 197 926 1387 1159 901 262 1553 Fragments arranged by size: 1553 1387 1159 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4176 493 Size: 3683 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6385 SphI G_CATG'C Cuts at: 1132 3743 1132 Size: 2611 3774 SspI AAT'ATT Cuts at: 5 52 4309 5 Size: 47 4257 2081 Fragments arranged by size: 4257 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6385 StyI C'CwwG_G Cuts at: 513 1065 2004 2487 2503 2572 3438 3462 3824 Size: 552 939 483 16 69 866 24 362 Cuts at: 3824 513 Size: 3074 Fragments arranged by size: 3074 939 866 552 483 362 69 24 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2360 4412 4429 4582 4767 5212 325 Size: 1255 780 2052 17 153 185 445 1498 Fragments arranged by size: 2052 1498 1255 780 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4631 6343 96 Size: 274 80 33 51 1443 2654 1712 138 Fragments arranged by size: 2654 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2375 2407 2667 2699 6345 6377 2375 Size: 32 260 32 3646 32 2383 Fragments arranged by size: 3646 2383 260 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2340 1316 Size: 1024 5361 XbaI T'CTAG_A Cuts at: 2234 3721 2234 Size: 1487 4898 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3454 3454 Size: 6385 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6385 XmaI C'CCGG_G Cuts at: 1870 3710 1870 Size: 1840 4545 XmnI GAAnn'nnTTC Cuts at: 4514 4514 Size: 6385 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4193 276 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI