(Circular) (Six-base) MAPSORT of: pFN21AB9426.seq Check: 3548 from: 1 to: 6754 pFN21AB9426 6754 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4564 278 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3609 3720 4097 1937 Size: 1672 111 377 4594 Fragments arranged by size: 4594 1672 377 111 Acc65I G'GTAC_C Cuts at: 4075 4075 Size: 6754 AclI AA'CG_TT Cuts at: 1563 4881 5254 1563 Size: 3318 373 3063 Fragments arranged by size: 3318 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2448 2810 3182 4817 6201 6469 1632 Size: 816 362 372 1635 1384 268 1917 Fragments arranged by size: 1917 1635 1384 816 372 362 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6557 AflIII A'CryG_T Cuts at: 1203 5653 6630 1203 Size: 4450 977 1327 Fragments arranged by size: 4450 1327 977 AgeI A'CCGG_T Cuts at: 5585 5585 Size: 6754 AhdI GACnn_n'nnGTC Cuts at: 5483 5483 Size: 6754 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6754 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6754 AlwNI CAG_nnn'CTG Cuts at: 2125 2326 4497 6069 2125 Size: 201 2171 1572 2810 Fragments arranged by size: 2810 2171 1572 201 ApaI G_GGCC'C Cuts at: 3865 3865 Size: 6754 ApaLI G'TGCA_C Cuts at: 2620 4811 5967 2620 Size: 2191 1156 3407 Fragments arranged by size: 3407 2191 1156 ApoI r'AATT_y Cuts at: 1493 4063 4289 4441 6550 1493 Size: 2570 226 152 2109 1697 Fragments arranged by size: 2570 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5308 160 Size: 872 4276 1606 Fragments arranged by size: 4276 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 AvaI C'yCGr_G Cuts at: 1870 1943 2249 2375 3915 4079 1870 Size: 73 306 126 1540 164 4545 Fragments arranged by size: 4545 1540 306 164 126 73 BamHI G'GATC_C Cuts at: 3219 3753 4084 4451 3219 Size: 534 331 367 5522 Fragments arranged by size: 5522 534 367 331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2015 2342 4075 5530 618 Size: 325 217 627 228 327 1733 1455 1842 Fragments arranged by size: 1842 1733 1455 627 327 325 228 217 BanII G_rGCy'C Cuts at: 1391 3373 3865 4073 1391 Size: 1982 492 208 4072 Fragments arranged by size: 4072 1982 492 208 BbeI G_GCGC'C Cuts at: 2346 2346 Size: 6754 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2195 2254 928 Size: 971 296 59 5428 Fragments arranged by size: 5428 971 296 59 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2611 2645 4945 4979 1903 Size: 34 674 34 2300 34 3678 Fragments arranged by size: 3678 2300 674 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4646 5856 4646 Size: 1210 5544 BclI T'GATC_A Cuts at: 1548 1548 Size: 6754 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5365 136 Size: 107 122 71 631 605 3693 1525 Fragments arranged by size: 3693 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3439 6749 3439 Size: 3310 3444 BlpI GC'TnA_GC Cuts at: 4171 4171 Size: 6754 Bme1580I G_kGCm'C Cuts at: 1790 2624 3865 4815 5971 1790 Size: 834 1241 950 1156 2573 Fragments arranged by size: 2573 1241 1156 950 834 BmgBI CAC'GTC Cuts at: 1318 3172 1318 Size: 1854 4900 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3868 4485 5443 Size: 416 53 83 399 24 2451 617 958 Cuts at: 5443 442 Size: 1753 Fragments arranged by size: 2451 1753 958 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6754 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 3555 3566 4023 5414 1239 Size: 1184 1132 11 457 1391 2579 Fragments arranged by size: 2579 1391 1184 1132 457 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3850 4879 5744 6042 3850 Size: 1029 865 298 4562 Fragments arranged by size: 4562 1029 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2439 2939 3132 5417 882 Size: 617 940 500 193 2285 2219 Fragments arranged by size: 2285 2219 940 617 500 193 BsaAI yAC'GTr Cuts at: 493 1204 2205 2535 4545 493 Size: 711 1001 330 2010 2702 Fragments arranged by size: 2702 2010 1001 711 330 BsaBI GATnn'nnATC Cuts at: 2732 6748 2732 Size: 4016 2738 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2273 2343 4561 4943 Size: 53 83 186 936 740 70 2218 382 Cuts at: 4943 5578 275 Size: 635 1451 Fragments arranged by size: 2218 1451 936 740 635 382 186 83 70 53 BsaWI w'CCGG_w Cuts at: 2448 5187 5585 5859 6006 6417 2448 Size: 2739 398 274 147 411 2785 Fragments arranged by size: 2785 2739 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6529 Fragments arranged by size: 6529 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 1186 Size: 1042 5712 BseYI C'CCAG_C Cuts at: 3864 5957 6453 3864 Size: 2093 496 4165 Fragments arranged by size: 4165 2093 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2290 1163 Size: 573 554 5627 Fragments arranged by size: 5627 573 554 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2340 3286 4204 4965 5993 1540 Size: 275 186 339 946 918 761 1028 2301 Fragments arranged by size: 2301 1028 946 918 761 339 275 186 BsiHKAI G_wGCw'C Cuts at: 2624 3373 3822 4815 4900 5971 2624 Size: 749 449 993 85 1071 3407 Fragments arranged by size: 3407 1071 993 749 449 85 BsmI GAATG_Cn' Cuts at: 3203 4265 4358 3203 Size: 1062 93 5599 Fragments arranged by size: 5599 1062 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2264 2264 Size: 6754 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2624 3373 3822 3865 4073 4815 4900 Size: 399 834 749 449 43 208 742 85 Cuts at: 4900 5971 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 834 749 742 449 399 208 85 43 BspEI T'CCGG_A Cuts at: 6417 6417 Size: 6754 BspHI T'CATG_A Cuts at: 3768 4643 6373 3768 Size: 875 1730 4149 Fragments arranged by size: 4149 1730 875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4109 844 Size: 3265 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4641 1394 Size: 429 2818 3507 Fragments arranged by size: 3507 2818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3529 3627 5249 5423 65 Size: 1183 2281 98 1622 174 1396 Fragments arranged by size: 2281 1622 1396 1183 174 98 BsrFI r'CCGG_y Cuts at: 1700 1810 2339 5398 5585 1700 Size: 110 529 3059 187 2869 Fragments arranged by size: 3059 2869 529 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6754 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6754 BssSI C'ACGA_G Cuts at: 2267 3366 3470 4814 5826 2267 Size: 1099 104 1344 1012 3195 Fragments arranged by size: 3195 1344 1099 1012 104 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6754 BstBI TT'CG_AA Cuts at: 5558 5558 Size: 6754 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6754 BstXI CCAn_nnnn'nTGG Cuts at: 2513 2920 3793 2513 Size: 407 873 5474 Fragments arranged by size: 5474 873 407 BstYI r'GATC_y Cuts at: 1972 2371 2451 3219 3439 3576 3753 4084 4451 Size: 399 80 768 220 137 177 331 367 Cuts at: 4451 4836 4853 6305 6391 6403 6749 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 768 399 385 367 346 331 220 177 137 86 80 17 12 BtgI C'CryG_G Cuts at: 513 1065 3534 513 Size: 552 2469 3733 Fragments arranged by size: 3733 2469 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3860 487 Size: 683 56 2634 3381 Fragments arranged by size: 3381 2634 683 56 BtsI GCAGTG_nn' Cuts at: 2067 2563 2795 4270 5064 5084 2067 Size: 496 232 1475 794 20 3737 Fragments arranged by size: 3737 1475 794 496 232 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6754 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6719 DraI TTT'AAA Cuts at: 4058 4058 Size: 6754 DraIII CAC_nnn'GTG Cuts at: 2296 2296 Size: 6754 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5761 809 Size: 507 4445 1802 Fragments arranged by size: 4445 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2242 2256 2298 Size: 54 1425 50 275 18 412 14 42 Cuts at: 2298 2529 3537 4201 5090 6719 8 Size: 231 1008 664 889 1629 43 Fragments arranged by size: 1629 1425 1008 889 664 412 275 231 54 50 43 42 18 14 EagI C'GGCC_G Cuts at: 1537 1812 4201 1537 Size: 275 2389 4090 Fragments arranged by size: 4090 2389 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 3951 4684 1353 Size: 755 1843 733 3423 Fragments arranged by size: 3423 1843 755 733 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5341 5715 5861 387 Size: 1064 138 3752 374 146 1280 Fragments arranged by size: 3752 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3371 3371 Size: 6754 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 2448 2810 3182 3555 3566 4023 Size: 393 791 25 362 372 373 11 457 Cuts at: 4023 4817 5414 6201 6469 1239 Size: 794 597 787 268 1524 Fragments arranged by size: 1524 794 791 787 597 457 393 373 372 362 268 25 11 EcoNI CCTnn'n_nnAGG Cuts at: 3692 3806 3692 Size: 114 6640 EcoRI G'AATT_C Cuts at: 4063 4441 4063 Size: 378 6376 FspI TGC'GCA Cuts at: 2333 5260 2333 Size: 2927 3827 HaeII r_GCGC'y Cuts at: 1678 2346 2479 5901 1678 Size: 668 133 3422 2531 Fragments arranged by size: 3422 2531 668 133 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2436 2468 3042 3074 3211 Size: 32 135 32 864 32 574 32 137 Cuts at: 3211 3243 3268 3300 3891 3909 3923 3941 5395 Size: 32 25 32 591 18 14 18 1454 Cuts at: 5395 5427 5469 5501 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1454 864 591 574 137 135 42 32 32 32 32 32 32 32 32 25 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 4098 4344 669 Size: 988 281 2160 246 3079 Fragments arranged by size: 3079 2160 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6754 HpaI GTT'AAC Cuts at: 4344 4344 Size: 6754 KasI G'GCGC_C Cuts at: 2342 2342 Size: 6754 KpnI G_GTAC'C Cuts at: 4079 4079 Size: 6754 MfeI C'AATT_G Cuts at: 3975 4353 3975 Size: 378 6376 MluI A'CGCG_T Cuts at: 6630 6630 Size: 6754 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2962 2981 3069 3444 5868 6052 1305 Size: 1657 19 88 375 2424 184 2007 Fragments arranged by size: 2424 2007 1657 375 184 88 19 MscI TGG'CCA Cuts at: 10 64 1489 1832 2300 2531 10 Size: 54 1425 343 468 231 4233 Fragments arranged by size: 4233 1425 468 343 231 54 MslI CAynn'nnrTG Cuts at: 518 1166 3080 3741 4712 5071 5230 518 Size: 648 1914 661 971 359 159 2042 Fragments arranged by size: 2042 1914 971 661 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2082 2122 3868 4170 4847 5995 6240 1606 Size: 476 40 1746 302 677 1148 245 2120 Fragments arranged by size: 2120 1746 1148 677 476 302 245 40 NarI GG'CG_CC Cuts at: 2343 2343 Size: 6754 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6202 NdeI CA'TA_TG Cuts at: 387 387 Size: 6754 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6754 NotI GC'GGCC_GC Cuts at: 4201 4201 Size: 6754 NspI r_CATG'y Cuts at: 1132 1229 4112 5657 1132 Size: 97 2883 1545 2229 Fragments arranged by size: 2883 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2278 1348 Size: 930 5824 PciI A'CATG_T Cuts at: 5653 5653 Size: 6754 PflMI CCAn_nnn'nTGG Cuts at: 1108 2082 2125 2296 1108 Size: 974 43 171 5566 Fragments arranged by size: 5566 974 171 43 PfoI T'CCnGG_A Cuts at: 2319 3582 2319 Size: 1263 5491 PmeI GTTT'AAAC Cuts at: 4058 4058 Size: 6754 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4823 4855 6336 6368 4823 Size: 32 1481 32 5209 Fragments arranged by size: 5209 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6754 PsiI TTA'TAA Cuts at: 4324 4324 Size: 6754 PspOMI G'GGCC_C Cuts at: 3861 3861 Size: 6754 PspXI vC'TCGA_Gb Cuts at: 2249 2249 Size: 6754 PstI C_TGCA'G Cuts at: 830 2073 2316 2570 3183 3388 3607 4106 830 Size: 1243 243 254 613 205 219 499 3478 Fragments arranged by size: 3478 1243 613 499 254 243 219 205 PvuI CG_AT'CG Cuts at: 2001 3286 2001 Size: 1285 5469 PvuII CAG'CTG Cuts at: 2122 3868 2122 Size: 1746 5008 SacI G_AGCT'C Cuts at: 3373 3373 Size: 6754 SalI G'TCGA_C Cuts at: 1936 4096 1936 Size: 2160 4594 SapI GCTCTTCn'nnn_ Cuts at: 2108 3951 2108 Size: 1843 4911 SbfI CC_TGCA'GG Cuts at: 4106 4106 Size: 6754 ScaI AGT'ACT Cuts at: 5002 6714 5002 Size: 1712 5042 SexAI A'CCwGG_T Cuts at: 2154 2154 Size: 6754 SfcI C'TryA_G Cuts at: 826 1045 2069 2312 2566 3179 3384 3433 3603 Size: 219 1024 243 254 613 205 49 170 Cuts at: 3603 4102 5237 5918 6109 826 Size: 499 1135 681 191 1471 Fragments arranged by size: 1471 1135 1024 681 613 499 254 243 219 205 191 170 49 SfoI GGC'GCC Cuts at: 2344 2344 Size: 6754 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6754 SmaI CCC'GGG Cuts at: 1872 4081 1872 Size: 2209 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 2375 3829 4858 5759 6021 Size: 197 926 306 126 1454 1029 901 262 Cuts at: 6021 820 Size: 1553 Fragments arranged by size: 1553 1454 1029 926 901 306 262 197 126 SnaBI TAC'GTA Cuts at: 493 4545 493 Size: 4052 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6754 SphI G_CATG'C Cuts at: 1132 4112 1132 Size: 2980 3774 SspI AAT'ATT Cuts at: 5 52 2686 2950 4678 5 Size: 47 2634 264 1728 2081 Fragments arranged by size: 2634 2081 1728 264 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6754 StyI C'CwwG_G Cuts at: 513 1065 4193 513 Size: 552 3128 3074 Fragments arranged by size: 3128 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2356 3224 4781 4798 4951 5136 5581 Size: 1255 776 868 1557 17 153 185 445 Cuts at: 5581 325 Size: 1498 Fragments arranged by size: 1557 1498 1255 868 776 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2669 5000 6712 Size: 274 80 33 51 1443 692 2331 1712 Cuts at: 6712 96 Size: 138 Fragments arranged by size: 2331 1712 1443 692 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6714 6746 6714 Size: 32 6722 Tth111I GACn'n_nGTC Cuts at: 1316 3455 1316 Size: 2139 4615 XbaI T'CTAG_A Cuts at: 4090 4090 Size: 6754 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2510 3790 2510 Size: 1280 5474 XhoI C'TCGA_G Cuts at: 1943 2249 2375 1943 Size: 306 126 6322 Fragments arranged by size: 6322 306 126 XmaI C'CCGG_G Cuts at: 1870 4079 1870 Size: 2209 4545 XmnI GAAnn'nnTTC Cuts at: 2950 4024 4883 2950 Size: 1074 859 4821 Fragments arranged by size: 4821 1074 859 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4562 276 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PsrI RsrII SacII SanDI SfiI SrfI SwaI