(Circular) (Six-base) MAPSORT of: pFN21AB9418.seq Check: 9389 from: 1 to: 6562 pFN21AB9418 6562 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2458 2458 Size: 6562 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4372 278 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3905 1937 Size: 1968 4594 Acc65I G'GTAC_C Cuts at: 3883 3883 Size: 6562 AclI AA'CG_TT Cuts at: 1563 4689 5062 1563 Size: 3126 373 3063 Fragments arranged by size: 3126 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2232 2409 2469 2919 3571 3651 3747 4625 Size: 600 177 60 450 652 80 96 878 Cuts at: 4625 6009 6277 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 878 652 600 450 268 177 96 80 60 AfeI AGC'GCT Cuts at: 2855 2855 Size: 6562 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6365 AflIII A'CryG_T Cuts at: 1203 2116 5461 6438 1203 Size: 913 3345 977 1327 Fragments arranged by size: 3345 1327 977 913 AgeI A'CCGG_T Cuts at: 5393 5393 Size: 6562 AhdI GACnn_n'nnGTC Cuts at: 5291 5291 Size: 6562 AleI CACnn'nnGTG Cuts at: 1166 2420 1166 Size: 1254 5308 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6562 AlwNI CAG_nnn'CTG Cuts at: 3386 4305 5877 3386 Size: 919 1572 4071 Fragments arranged by size: 4071 1572 919 ApaI G_GGCC'C Cuts at: 3506 3506 Size: 6562 ApaLI G'TGCA_C Cuts at: 4619 5775 4619 Size: 1156 5406 ApoI r'AATT_y Cuts at: 1493 2042 3049 3238 3871 4097 4249 6358 1493 Size: 549 1007 189 633 226 152 2109 1697 Fragments arranged by size: 2109 1697 1007 633 549 226 189 152 AseI AT'TA_AT Cuts at: 160 1032 5116 160 Size: 872 4084 1606 Fragments arranged by size: 4084 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 AvaI C'yCGr_G Cuts at: 1870 1943 3887 1870 Size: 73 1944 4545 Fragments arranged by size: 4545 1944 73 AvrII C'CTAG_G Cuts at: 3302 3302 Size: 6562 BamHI G'GATC_C Cuts at: 2911 3892 4259 2911 Size: 981 367 5214 Fragments arranged by size: 5214 981 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3883 5338 618 Size: 325 217 627 2096 1455 1842 Fragments arranged by size: 2096 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2175 3482 3506 3881 1391 Size: 784 1307 24 375 4072 Fragments arranged by size: 4072 1307 784 375 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2925 928 Size: 971 1026 4565 Fragments arranged by size: 4565 1026 971 BbvCI CC'TCA_GC Cuts at: 2310 2310 Size: 6562 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4753 4787 1903 Size: 34 2816 34 3678 Fragments arranged by size: 3678 2816 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4454 5664 4454 Size: 1210 5352 BclI T'GATC_A Cuts at: 1548 1548 Size: 6562 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5173 136 Size: 107 122 71 631 605 3501 1525 Fragments arranged by size: 3501 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2887 6557 2887 Size: 3670 2892 BlpI GC'TnA_GC Cuts at: 3979 3979 Size: 6562 Bme1580I G_kGCm'C Cuts at: 1790 3506 4623 5779 1790 Size: 1716 1117 1156 2573 Fragments arranged by size: 2573 1716 1156 1117 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6562 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2405 4293 5251 Size: 416 53 83 399 24 988 1888 958 Cuts at: 5251 442 Size: 1753 Fragments arranged by size: 1888 1753 988 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6562 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2642 5222 1239 Size: 1403 2580 2579 Fragments arranged by size: 2580 2579 1403 Bpu10I CC'TnA_GC Cuts at: 2310 2310 Size: 6562 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3666 4687 5552 5850 3666 Size: 1021 865 298 4378 Fragments arranged by size: 4378 1021 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2783 5225 882 Size: 617 1284 2442 2219 Fragments arranged by size: 2442 2219 1284 617 BsaAI yAC'GTr Cuts at: 493 1204 4353 493 Size: 711 3149 2702 Fragments arranged by size: 3149 2702 711 BsaBI GATnn'nnATC Cuts at: 2910 6556 2910 Size: 3646 2916 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4369 4751 5386 275 Size: 53 83 186 936 2836 382 635 1451 Fragments arranged by size: 2836 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3153 4995 5393 5667 5814 6225 3153 Size: 1842 398 274 147 411 3490 Fragments arranged by size: 3490 1842 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3115 3145 3601 3631 1178 Size: 30 165 30 1712 30 456 30 4109 Fragments arranged by size: 4109 1712 456 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2309 3036 3474 3609 1186 Size: 1123 727 438 135 4139 Fragments arranged by size: 4139 1123 727 438 135 BseYI C'CCAG_C Cuts at: 2566 2697 3144 5765 6261 2566 Size: 131 447 2621 496 2867 Fragments arranged by size: 2867 2621 496 447 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3137 3582 1163 Size: 573 1401 445 4143 Fragments arranged by size: 4143 1401 573 445 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2990 4012 4773 5801 1540 Size: 275 186 989 1022 761 1028 2301 Fragments arranged by size: 2301 1028 1022 989 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2806 4623 4708 5779 2806 Size: 1817 85 1071 3589 Fragments arranged by size: 3589 1817 1071 85 BsmI GAATG_Cn' Cuts at: 4073 4166 4073 Size: 93 6469 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2806 3482 3506 3881 4623 4708 Size: 399 385 631 676 24 375 742 85 Cuts at: 4708 5779 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 676 631 399 385 375 85 24 BspEI T'CCGG_A Cuts at: 3153 6225 3153 Size: 3072 3490 BspHI T'CATG_A Cuts at: 2272 4451 6181 2272 Size: 2179 1730 2653 Fragments arranged by size: 2653 2179 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2458 3371 3511 3917 844 Size: 1614 913 140 406 3489 Fragments arranged by size: 3489 1614 913 406 140 BsrBI CCG'CTC Cuts at: 1394 1823 4449 1394 Size: 429 2626 3507 Fragments arranged by size: 3507 2626 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3541 3559 5057 5231 65 Size: 1183 2293 18 1498 174 1396 Fragments arranged by size: 2293 1498 1396 1183 174 18 BsrFI r'CCGG_y Cuts at: 1700 1810 2150 2985 5206 5393 1700 Size: 110 340 835 2221 187 2869 Fragments arranged by size: 2869 2221 835 340 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6562 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6562 BssSI C'ACGA_G Cuts at: 4622 5634 4622 Size: 1012 5550 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6562 BstBI TT'CG_AA Cuts at: 5366 5366 Size: 6562 BstEII G'GTnAC_C Cuts at: 1184 3607 1184 Size: 2423 4139 BstYI r'GATC_y Cuts at: 1972 2887 2911 2938 3073 3370 3389 3892 4259 Size: 915 24 27 135 297 19 503 367 Cuts at: 4259 4644 4661 6113 6199 6211 6557 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 915 503 385 367 346 297 135 86 27 24 19 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2793 513 Size: 552 939 789 4282 Fragments arranged by size: 4282 939 789 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5823 Fragments arranged by size: 5823 683 56 BtsI GCAGTG_nn' Cuts at: 2651 2745 4078 4872 4892 2651 Size: 94 1333 794 20 4321 Fragments arranged by size: 4321 1333 794 94 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6562 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6527 DraI TTT'AAA Cuts at: 2719 2872 3866 2719 Size: 153 994 5415 Fragments arranged by size: 5415 994 153 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5569 809 Size: 507 4253 1802 Fragments arranged by size: 4253 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4009 4898 6527 Size: 54 1425 50 275 18 2179 889 1629 Cuts at: 6527 8 Size: 43 Fragments arranged by size: 2179 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4009 1537 Size: 275 2197 4090 Fragments arranged by size: 4090 2197 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2163 2794 3155 4492 1353 Size: 810 631 361 1337 3423 Fragments arranged by size: 3423 1337 810 631 361 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5149 5523 5669 387 Size: 1064 138 3560 374 146 1280 Fragments arranged by size: 3560 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2409 2469 2642 2919 3571 3651 Size: 393 600 177 60 173 277 652 80 Cuts at: 3651 3747 4625 5222 6009 6277 1239 Size: 96 878 597 787 268 1524 Fragments arranged by size: 1524 878 787 652 600 597 393 277 268 177 173 96 80 60 EcoO109I rG'GnC_Cy Cuts at: 3503 3523 3503 Size: 20 6542 EcoRI G'AATT_C Cuts at: 3049 3871 4249 3049 Size: 822 378 5362 Fragments arranged by size: 5362 822 378 FspI TGC'GCA Cuts at: 5068 5068 Size: 6562 HaeII r_GCGC'y Cuts at: 1678 2857 5709 1678 Size: 1179 2852 2531 Fragments arranged by size: 2852 2531 1179 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3601 3633 5203 5235 5277 Size: 32 135 32 2029 32 1570 32 42 Cuts at: 5277 5309 1373 Size: 32 2626 Fragments arranged by size: 2626 2029 1570 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3906 4152 669 Size: 988 281 1968 246 3079 Fragments arranged by size: 3079 1968 988 281 246 HindIII A'AGCT_T Cuts at: 748 2262 748 Size: 1514 5048 HpaI GTT'AAC Cuts at: 4152 4152 Size: 6562 KpnI G_GTAC'C Cuts at: 3887 3887 Size: 6562 MfeI C'AATT_G Cuts at: 2289 3492 4161 2289 Size: 1203 669 4690 Fragments arranged by size: 4690 1203 669 MluI A'CGCG_T Cuts at: 6438 6438 Size: 6562 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2044 3299 5676 5860 1305 Size: 739 1255 2377 184 2007 Fragments arranged by size: 2377 2007 1255 739 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4740 Fragments arranged by size: 4740 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2420 4520 4879 5038 518 Size: 648 1254 2100 359 159 2042 Fragments arranged by size: 2100 2042 1254 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3634 3978 4655 5803 6048 1606 Size: 2028 344 677 1148 245 2120 Fragments arranged by size: 2120 2028 1148 677 344 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2793 513 Size: 552 939 789 4282 Fragments arranged by size: 4282 939 789 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6562 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6562 NotI GC'GGCC_GC Cuts at: 4009 4009 Size: 6562 NspI r_CATG'y Cuts at: 1132 1229 2120 3920 5465 1132 Size: 97 891 1800 1545 2229 Fragments arranged by size: 2229 1800 1545 891 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6562 PciI A'CATG_T Cuts at: 2116 5461 2116 Size: 3345 3217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2751 1108 Size: 1643 4919 PfoI T'CCnGG_A Cuts at: 2658 2658 Size: 6562 PmeI GTTT'AAAC Cuts at: 3866 3866 Size: 6562 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4631 4663 6144 6176 4631 Size: 32 1481 32 5017 Fragments arranged by size: 5017 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3523 3523 Size: 6562 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6562 PsiI TTA'TAA Cuts at: 4132 4132 Size: 6562 PspOMI G'GGCC_C Cuts at: 3502 3502 Size: 6562 PstI C_TGCA'G Cuts at: 830 3465 3914 830 Size: 2635 449 3478 Fragments arranged by size: 3478 2635 449 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6562 PvuII CAG'CTG Cuts at: 3634 3634 Size: 6562 SalI G'TCGA_C Cuts at: 1936 3904 1936 Size: 1968 4594 SapI GCTCTTCn'nnn_ Cuts at: 2163 2794 3155 2163 Size: 631 361 5570 Fragments arranged by size: 5570 631 361 SbfI CC_TGCA'GG Cuts at: 3914 3914 Size: 6562 ScaI AGT'ACT Cuts at: 3441 4810 6522 3441 Size: 1369 1712 3481 Fragments arranged by size: 3481 1712 1369 SfcI C'TryA_G Cuts at: 826 1045 3030 3461 3910 5045 5726 5917 826 Size: 219 1985 431 449 1135 681 191 1471 Fragments arranged by size: 1985 1471 1135 681 449 431 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6562 SmaI CCC'GGG Cuts at: 1872 3889 1872 Size: 2017 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3681 4666 5567 5829 820 Size: 197 926 1738 985 901 262 1553 Fragments arranged by size: 1738 1553 985 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4353 493 Size: 3860 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6562 SphI G_CATG'C Cuts at: 1132 3920 1132 Size: 2788 3774 SspI AAT'ATT Cuts at: 5 52 4486 5 Size: 47 4434 2081 Fragments arranged by size: 4434 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6562 StyI C'CwwG_G Cuts at: 513 1065 2004 2793 3302 4001 513 Size: 552 939 789 509 699 3074 Fragments arranged by size: 3074 939 789 699 552 509 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2432 3660 4589 4606 4759 4944 5389 Size: 1255 852 1228 929 17 153 185 445 Cuts at: 5389 325 Size: 1498 Fragments arranged by size: 1498 1255 1228 929 852 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3169 3178 3439 Size: 274 80 33 51 1443 1192 9 261 Cuts at: 3439 4808 6520 96 Size: 1369 1712 138 Fragments arranged by size: 1712 1443 1369 1192 274 261 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3115 3147 6522 6554 3115 Size: 32 3375 32 3123 Fragments arranged by size: 3375 3123 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6562 XbaI T'CTAG_A Cuts at: 3898 3898 Size: 6562 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3076 3076 Size: 6562 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6562 XmaI C'CCGG_G Cuts at: 1870 3887 1870 Size: 2017 4545 XmnI GAAnn'nnTTC Cuts at: 2118 4691 2118 Size: 2573 3989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4370 276 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI