(Circular) (Six-base) MAPSORT of: pFN21AB9339.seq Check: 6266 from: 1 to: 8497 pFN21AB9339 8497 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3424 4183 3424 Size: 759 7738 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2692 4641 6307 278 Size: 53 83 186 936 1156 1949 1666 2468 Fragments arranged by size: 2468 1949 1666 1156 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3822 5840 1937 Size: 1885 2018 4594 Fragments arranged by size: 4594 2018 1885 Acc65I G'GTAC_C Cuts at: 5818 5818 Size: 8497 AclI AA'CG_TT Cuts at: 1563 6624 6997 1563 Size: 5061 373 3063 Fragments arranged by size: 5061 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3814 3826 4922 5400 5683 6560 7944 8212 Size: 2182 12 1096 478 283 877 1384 268 Cuts at: 8212 1632 Size: 1917 Fragments arranged by size: 2182 1917 1384 1096 877 478 283 268 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8300 AflIII A'CryG_T Cuts at: 1203 4836 5229 7396 8373 1203 Size: 3633 393 2167 977 1327 Fragments arranged by size: 3633 2167 1327 977 393 AgeI A'CCGG_T Cuts at: 3656 7328 3656 Size: 3672 4825 AhdI GACnn_n'nnGTC Cuts at: 4567 5455 7226 4567 Size: 888 1771 5838 Fragments arranged by size: 5838 1771 888 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8497 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4908 4940 4908 Size: 32 8465 AlwNI CAG_nnn'CTG Cuts at: 3617 3727 3868 5035 5539 6240 7812 3617 Size: 110 141 1167 504 701 1572 4302 Fragments arranged by size: 4302 1572 1167 701 504 141 110 ApaI G_GGCC'C Cuts at: 4513 4513 Size: 8497 ApaLI G'TGCA_C Cuts at: 6554 7710 6554 Size: 1156 7341 ApoI r'AATT_y Cuts at: 1493 2114 2273 2487 3227 3623 4790 5806 6032 Size: 621 159 214 740 396 1167 1016 226 Cuts at: 6032 6184 8293 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1167 1016 740 621 396 226 214 159 152 AseI AT'TA_AT Cuts at: 160 1032 7051 160 Size: 872 6019 1606 Fragments arranged by size: 6019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8497 AvaI C'yCGr_G Cuts at: 1870 1943 2949 3425 3983 4324 4413 5043 5822 Size: 73 1006 476 558 341 89 630 779 Cuts at: 5822 1870 Size: 4545 Fragments arranged by size: 4545 1006 779 630 558 476 341 89 73 BamHI G'GATC_C Cuts at: 5827 6194 5827 Size: 367 8130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2935 5380 5818 7273 618 Size: 325 217 627 1148 2445 438 1455 1842 Fragments arranged by size: 2445 1842 1455 1148 627 438 325 217 BanII G_rGCy'C Cuts at: 1391 2708 2757 2823 4196 4513 4761 4872 5130 Size: 1317 49 66 1373 317 248 111 258 Cuts at: 5130 5195 5816 1391 Size: 65 621 4072 Fragments arranged by size: 4072 1373 1317 621 317 258 248 111 66 65 49 BbeI G_GCGC'C Cuts at: 2939 5384 2939 Size: 2445 6052 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2601 4915 5798 928 Size: 971 702 2314 883 3627 Fragments arranged by size: 3627 2314 971 883 702 BbvCI CC'TCA_GC Cuts at: 2166 3267 3610 4459 5715 2166 Size: 1101 343 849 1256 4948 Fragments arranged by size: 4948 1256 1101 849 343 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5428 5462 6688 6722 1903 Size: 34 3491 34 1226 34 3678 Fragments arranged by size: 3678 3491 1226 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6389 7599 6389 Size: 1210 7287 BclI T'GATC_A Cuts at: 1548 1548 Size: 8497 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4047 5477 7108 Size: 107 122 71 631 605 2375 1430 1631 Cuts at: 7108 136 Size: 1525 Fragments arranged by size: 2375 1631 1525 1430 631 605 122 107 71 BglII A'GATC_T Cuts at: 8492 8492 Size: 8497 BlpI GC'TnA_GC Cuts at: 2500 3125 4949 5124 5914 2500 Size: 625 1824 175 790 5083 Fragments arranged by size: 5083 1824 790 625 175 Bme1580I G_kGCm'C Cuts at: 1790 2365 4065 4513 6558 7714 1790 Size: 575 1700 448 2045 1156 2573 Fragments arranged by size: 2573 2045 1700 1156 575 448 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8497 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2712 2808 5200 Size: 416 53 83 399 24 1295 96 2392 Cuts at: 5200 5248 6228 7186 442 Size: 48 980 958 1753 Fragments arranged by size: 2392 1753 1295 980 958 416 399 96 83 53 48 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8497 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3754 4810 3754 Size: 1056 7441 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2354 2438 2471 2977 3218 3749 4553 Size: 1037 78 84 33 506 241 531 804 Cuts at: 4553 5008 5417 7157 1239 Size: 455 409 1740 2579 Fragments arranged by size: 2579 1740 1037 804 531 506 455 409 241 84 78 33 Bpu10I CC'TnA_GC Cuts at: 2166 2324 3267 3287 3610 4002 4459 5715 2166 Size: 158 943 20 323 392 457 1256 4948 Fragments arranged by size: 4948 1256 943 457 392 323 158 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4595 6622 7487 7785 4595 Size: 2027 865 298 5307 Fragments arranged by size: 5307 2027 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2310 3327 7160 882 Size: 617 811 1017 3833 2219 Fragments arranged by size: 3833 2219 1017 811 617 BsaAI yAC'GTr Cuts at: 493 1204 6288 493 Size: 711 5084 2702 Fragments arranged by size: 5084 2702 711 BsaBI GATnn'nnATC Cuts at: 8491 8491 Size: 8497 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2689 2936 3770 4473 Size: 53 83 186 936 1156 247 834 703 Cuts at: 4473 4638 5381 6304 6686 7321 275 Size: 165 743 923 382 635 1451 Fragments arranged by size: 1451 1156 936 923 834 743 703 635 382 247 186 165 83 53 BsaWI w'CCGG_w Cuts at: 3656 4968 5244 6930 7328 7602 7749 8160 3656 Size: 1312 276 1686 398 274 147 411 3993 Fragments arranged by size: 3993 1686 1312 411 398 276 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3305 3335 3405 3435 5330 Size: 30 165 30 1902 30 70 30 1895 Cuts at: 5330 5360 1178 Size: 30 4315 Fragments arranged by size: 4315 1902 1895 165 70 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 2813 2856 3669 3800 3928 4141 4412 Size: 1165 462 43 813 131 128 213 271 Cuts at: 4412 4431 4565 4769 5198 5732 1186 Size: 19 134 204 429 534 3951 Fragments arranged by size: 3951 1165 813 534 462 429 271 213 204 134 131 128 43 19 BseYI C'CCAG_C Cuts at: 2746 3369 4232 4430 7700 8196 2746 Size: 623 863 198 3270 496 3047 Fragments arranged by size: 3270 3047 863 623 496 198 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2492 3881 5422 1163 Size: 573 756 1389 1541 4238 Fragments arranged by size: 4238 1541 1389 756 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5947 6708 7736 1540 Size: 275 186 3946 761 1028 2301 Fragments arranged by size: 3946 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2757 2823 3179 3542 3786 4196 4761 4872 4968 Size: 66 356 363 244 410 565 111 96 Cuts at: 4968 5130 5220 6558 6643 7714 2757 Size: 162 90 1338 85 1071 3540 Fragments arranged by size: 3540 1338 1071 565 410 363 356 244 162 111 96 90 85 66 BsmI GAATG_Cn' Cuts at: 2877 4718 6008 6101 2877 Size: 1841 1290 93 5273 Fragments arranged by size: 5273 1841 1290 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2700 4027 4488 2700 Size: 1327 461 6709 Fragments arranged by size: 6709 1327 461 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2365 2708 2757 2823 3179 3542 3786 Size: 399 575 343 49 66 356 363 244 Cuts at: 3786 4065 4196 4513 4761 4872 4968 5130 5195 Size: 279 131 317 248 111 96 162 65 Cuts at: 5195 5220 5816 6558 6643 7714 1391 Size: 25 596 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 596 575 399 363 356 343 317 279 248 244 162 131 111 96 85 66 65 49 25 BspEI T'CCGG_A Cuts at: 4968 8160 4968 Size: 3192 5305 BspHI T'CATG_A Cuts at: 5313 6386 8116 5313 Size: 1073 1730 5694 Fragments arranged by size: 5694 1730 1073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3424 3540 4183 5852 844 Size: 2580 116 643 1669 3489 Fragments arranged by size: 3489 2580 1669 643 116 BsrBI CCG'CTC Cuts at: 1394 1823 2010 3493 4001 6384 1394 Size: 429 187 1483 508 2383 3507 Fragments arranged by size: 3507 2383 1483 508 429 187 BsrDI GCAATG_nn' Cuts at: 65 1248 2909 4945 6992 7166 65 Size: 1183 1661 2036 2047 174 1396 Fragments arranged by size: 2047 2036 1661 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3656 4041 4220 4426 7141 7328 1700 Size: 110 1846 385 179 206 2715 187 2869 Fragments arranged by size: 2869 2715 1846 385 206 187 179 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8497 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8497 BssSI C'ACGA_G Cuts at: 6557 7569 6557 Size: 1012 7485 BstAPI GCAn_nnn'nTGC Cuts at: 794 5634 794 Size: 4840 3657 BstBI TT'CG_AA Cuts at: 7301 7301 Size: 8497 BstEII G'GTnAC_C Cuts at: 1184 3659 1184 Size: 2475 6022 BstXI CCAn_nnnn'nTGG Cuts at: 2293 3077 4240 2293 Size: 784 1163 6550 Fragments arranged by size: 6550 1163 784 BstYI r'GATC_y Cuts at: 1972 2251 2781 4453 4649 4896 5827 6194 6579 Size: 279 530 1672 196 247 931 367 385 Cuts at: 6579 6596 8048 8134 8146 8492 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1672 1452 931 530 385 367 346 279 247 196 86 17 12 BstZ17I GTA'TAC Cuts at: 3823 3823 Size: 8497 Bsu36I CC'TnA_GG Cuts at: 5151 5151 Size: 8497 BtgI C'CryG_G Cuts at: 513 1065 2835 4215 4239 4642 513 Size: 552 1770 1380 24 403 4368 Fragments arranged by size: 4368 1770 1380 552 403 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7758 Fragments arranged by size: 7758 683 56 BtsI GCAGTG_nn' Cuts at: 3179 4000 4507 6013 6807 6827 3179 Size: 821 507 1506 794 20 4849 Fragments arranged by size: 4849 1506 821 794 507 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2892 2927 529 Size: 35 2328 35 6099 Fragments arranged by size: 6099 2328 35 35 DraI TTT'AAA Cuts at: 5801 5801 Size: 8497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2803 7504 809 Size: 507 1487 4701 1802 Fragments arranged by size: 4701 1802 1487 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3361 4218 4384 Size: 54 1425 50 275 18 1531 857 166 Cuts at: 4384 5355 5427 5944 6833 8462 8 Size: 971 72 517 889 1629 43 Fragments arranged by size: 1629 1531 1425 971 889 857 517 275 166 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5944 1537 Size: 275 4132 4090 Fragments arranged by size: 4132 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2092 2515 2838 4159 4887 5135 5183 5781 Size: 739 423 323 1321 728 248 48 598 Cuts at: 5781 6427 1353 Size: 646 3423 Fragments arranged by size: 3423 1321 739 728 646 598 423 323 248 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3476 4119 4131 4916 7084 7458 Size: 1064 138 1887 643 12 785 2168 374 Cuts at: 7458 7604 387 Size: 146 1280 Fragments arranged by size: 2168 1887 1280 1064 785 643 374 146 138 12 EcoICRI GAG'CTC Cuts at: 2755 2821 4194 4759 4870 5128 2755 Size: 66 1373 565 111 258 6124 Fragments arranged by size: 6124 1373 565 258 111 66 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2354 2438 2471 2977 3218 3749 Size: 393 644 78 84 33 506 241 531 Cuts at: 3749 3814 3826 4553 4922 5008 5400 5417 5683 Size: 65 12 727 369 86 392 17 266 Cuts at: 5683 6560 7157 7944 8212 1239 Size: 877 597 787 268 1524 Fragments arranged by size: 1524 877 787 727 644 597 531 506 393 392 369 268 266 241 86 84 78 65 33 17 12 EcoNI CCTnn'n_nnAGG Cuts at: 3815 4985 5489 5714 3815 Size: 1170 504 225 6598 Fragments arranged by size: 6598 1170 504 225 EcoO109I rG'GnC_Cy Cuts at: 3529 4006 4086 4509 4510 4737 5067 5422 5536 Size: 477 80 423 1 227 330 355 114 Cuts at: 5536 5722 3529 Size: 186 6304 Fragments arranged by size: 6304 477 423 355 330 227 186 114 80 1 EcoRI G'AATT_C Cuts at: 2487 3227 5806 6184 2487 Size: 740 2579 378 4800 Fragments arranged by size: 4800 2579 740 378 FspI TGC'GCA Cuts at: 7003 7003 Size: 8497 HaeII r_GCGC'y Cuts at: 1678 2939 5384 7644 1678 Size: 1261 2445 2260 2531 Fragments arranged by size: 2531 2445 2260 1261 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3131 3163 3305 3337 3503 Size: 32 135 32 1559 32 142 32 166 Cuts at: 3503 3535 4553 4585 5441 5473 7138 7170 7212 Size: 32 1018 32 856 32 1665 32 42 Cuts at: 7212 7244 1373 Size: 32 2626 Fragments arranged by size: 2626 1665 1559 1018 856 166 142 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2814 3034 3654 4887 5841 6087 Size: 988 281 876 220 620 1233 954 246 Cuts at: 6087 669 Size: 3079 Fragments arranged by size: 3079 1233 988 954 876 620 281 246 220 HindIII A'AGCT_T Cuts at: 748 4119 748 Size: 3371 5126 HpaI GTT'AAC Cuts at: 6087 6087 Size: 8497 KasI G'GCGC_C Cuts at: 2935 5380 2935 Size: 2445 6052 KpnI G_GTAC'C Cuts at: 5822 5822 Size: 8497 MfeI C'AATT_G Cuts at: 2400 6096 2400 Size: 3696 4801 MluI A'CGCG_T Cuts at: 8373 8373 Size: 8497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2723 2986 4527 7611 7795 1305 Size: 1418 263 1541 3084 184 2007 Fragments arranged by size: 3084 2007 1541 1418 263 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4386 5357 10 Size: 54 1425 343 2554 971 3150 Fragments arranged by size: 3150 2554 1425 971 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2882 3075 5088 5255 6455 6814 6973 Size: 648 1716 193 2013 167 1200 359 159 Cuts at: 6973 518 Size: 2042 Fragments arranged by size: 2042 2013 1716 1200 648 359 193 167 159 MspA1I CmG'CkG Cuts at: 1606 2165 3145 3349 3901 3907 4463 4470 4644 Size: 559 980 204 552 6 556 7 174 Cuts at: 4644 5913 6590 7738 7983 1606 Size: 1269 677 1148 245 2120 Fragments arranged by size: 2120 1269 1148 980 677 559 556 552 245 204 174 7 6 NaeI GCC'GGC Cuts at: 4043 4043 Size: 8497 NarI GG'CG_CC Cuts at: 2936 5381 2936 Size: 2445 6052 NcoI C'CATG_G Cuts at: 513 1065 4215 513 Size: 552 3150 4795 Fragments arranged by size: 4795 3150 552 NdeI CA'TA_TG Cuts at: 387 3076 387 Size: 2689 5808 NgoMIV G'CCGG_C Cuts at: 4041 4041 Size: 8497 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8497 NotI GC'GGCC_GC Cuts at: 5944 5944 Size: 8497 NspI r_CATG'y Cuts at: 1132 1229 3709 3721 5233 5632 5662 5855 7400 Size: 97 2480 12 1512 399 30 193 1545 Cuts at: 7400 1132 Size: 2229 Fragments arranged by size: 2480 2229 1545 1512 399 193 97 30 12 PasI CC'CwG_GG Cuts at: 1348 3392 4478 1348 Size: 2044 1086 5367 Fragments arranged by size: 5367 2044 1086 PciI A'CATG_T Cuts at: 5229 7396 5229 Size: 2167 6330 PflMI CCAn_nnn'nTGG Cuts at: 1108 3332 4430 5035 5601 1108 Size: 2224 1098 605 566 4004 Fragments arranged by size: 4004 2224 1098 605 566 PfoI T'CCnGG_A Cuts at: 3179 4980 3179 Size: 1801 6696 PmeI GTTT'AAAC Cuts at: 5801 5801 Size: 8497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5277 5309 6566 6598 8079 8111 5277 Size: 32 1257 32 1481 32 5663 Fragments arranged by size: 5663 1481 1257 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3529 4086 4737 5067 5536 3529 Size: 557 651 330 469 6490 Fragments arranged by size: 6490 651 557 469 330 PshAI GACnn'nnGTC Cuts at: 1613 2692 1613 Size: 1079 7418 PsiI TTA'TAA Cuts at: 5681 6067 5681 Size: 386 8111 PspOMI G'GGCC_C Cuts at: 4509 4509 Size: 8497 PspXI vC'TCGA_Gb Cuts at: 4324 4324 Size: 8497 PstI C_TGCA'G Cuts at: 830 3110 3479 3537 3912 4776 5535 5849 830 Size: 2280 369 58 375 864 759 314 3478 Fragments arranged by size: 3478 2280 864 759 375 369 314 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8497 PvuII CAG'CTG Cuts at: 2165 3145 3349 3907 2165 Size: 980 204 558 6755 Fragments arranged by size: 6755 980 558 204 SacI G_AGCT'C Cuts at: 2757 2823 4196 4761 4872 5130 2757 Size: 66 1373 565 111 258 6124 Fragments arranged by size: 6124 1373 565 258 111 66 SacII CC_GC'GG Cuts at: 4645 4645 Size: 8497 SalI G'TCGA_C Cuts at: 1936 5839 1936 Size: 3903 4594 SapI GCTCTTCn'nnn_ Cuts at: 5135 5183 5135 Size: 48 8449 SbfI CC_TGCA'GG Cuts at: 5535 5849 5535 Size: 314 8183 ScaI AGT'ACT Cuts at: 5435 6745 8457 5435 Size: 1310 1712 5475 Fragments arranged by size: 5475 1712 1310 SfcI C'TryA_G Cuts at: 826 1045 2518 3106 3475 3533 3908 4732 4772 Size: 219 1473 588 369 58 375 824 40 Cuts at: 4772 5531 5845 6980 7661 7852 826 Size: 759 314 1135 681 191 1471 Fragments arranged by size: 1473 1471 1135 824 759 681 588 375 369 314 219 191 58 40 SfoI GGC'GCC Cuts at: 2937 5382 2937 Size: 2445 6052 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8497 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8497 SmaI CCC'GGG Cuts at: 1872 5045 5824 1872 Size: 3173 779 4545 Fragments arranged by size: 4545 3173 779 SmlI C'TyrA_G Cuts at: 820 1017 1943 4324 4413 4610 6601 7502 7764 Size: 197 926 2381 89 197 1991 901 262 Cuts at: 7764 820 Size: 1553 Fragments arranged by size: 2381 1991 1553 926 901 262 197 197 89 SnaBI TAC'GTA Cuts at: 493 6288 493 Size: 5795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8497 SphI G_CATG'C Cuts at: 1132 5632 5855 1132 Size: 4500 223 3774 Fragments arranged by size: 4500 3774 223 SspI AAT'ATT Cuts at: 5 52 6421 5 Size: 47 6369 2081 Fragments arranged by size: 6369 2081 47 StuI AGG'CCT Cuts at: 1517 3914 1517 Size: 2397 6100 StyI C'CwwG_G Cuts at: 513 1065 2247 4215 5936 513 Size: 552 1182 1968 1721 3074 Fragments arranged by size: 3074 1968 1721 1182 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2140 2932 3620 6524 6541 6694 6879 Size: 1255 560 792 688 2904 17 153 185 Cuts at: 6879 7324 325 Size: 445 1498 Fragments arranged by size: 2904 1498 1255 792 688 560 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4707 4776 5433 Size: 274 80 33 51 1443 2730 69 657 Cuts at: 5433 6743 8455 96 Size: 1310 1712 138 Fragments arranged by size: 2730 1712 1443 1310 657 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3405 3437 4106 4138 5489 5521 8457 8489 3405 Size: 32 669 32 1351 32 2936 32 3413 Fragments arranged by size: 3413 2936 1351 669 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8497 XbaI T'CTAG_A Cuts at: 5833 5833 Size: 8497 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2104 2290 2446 2464 3077 3353 5032 5392 2104 Size: 186 156 18 613 276 1679 360 5209 Fragments arranged by size: 5209 1679 613 360 276 186 156 18 XhoI C'TCGA_G Cuts at: 1943 4324 4413 1943 Size: 2381 89 6027 Fragments arranged by size: 6027 2381 89 XmaI C'CCGG_G Cuts at: 1870 5043 5822 1870 Size: 3173 779 4545 Fragments arranged by size: 4545 3173 779 XmnI GAAnn'nnTTC Cuts at: 4838 5339 6626 4838 Size: 501 1287 6709 Fragments arranged by size: 6709 1287 501 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2690 4639 6305 276 Size: 53 83 186 936 1156 1949 1666 2468 Fragments arranged by size: 2468 1949 1666 1156 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SanDI SexAI SfiI SrfI SwaI