(Circular) (Six-base) MAPSORT of: pFN21AB9288.seq Check: 8970 from: 1 to: 7798 pFN21AB9288 7798 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2007 2007 Size: 7798 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5608 278 Size: 53 83 186 936 4072 2468 Fragments arranged by size: 4072 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5141 1937 Size: 3204 4594 Acc65I G'GTAC_C Cuts at: 5119 5119 Size: 7798 AclI AA'CG_TT Cuts at: 1563 4519 5925 6298 1563 Size: 2956 1406 373 3063 Fragments arranged by size: 3063 2956 1406 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 2161 2187 3189 3250 3319 3385 3973 Size: 425 104 26 1002 61 69 66 588 Cuts at: 3973 4384 5032 5861 7245 7513 1632 Size: 411 648 829 1384 268 1917 Fragments arranged by size: 1917 1384 1002 829 648 588 425 411 268 104 69 66 61 26 AfeI AGC'GCT Cuts at: 4604 4604 Size: 7798 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7601 AflIII A'CryG_T Cuts at: 1203 3202 3384 4553 6697 7674 1203 Size: 1999 182 1169 2144 977 1327 Fragments arranged by size: 2144 1999 1327 1169 977 182 AgeI A'CCGG_T Cuts at: 6629 6629 Size: 7798 AhdI GACnn_n'nnGTC Cuts at: 3486 6527 3486 Size: 3041 4757 AleI CACnn'nnGTG Cuts at: 1166 3423 3614 4116 1166 Size: 2257 191 502 4848 Fragments arranged by size: 4848 2257 502 191 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7798 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2801 2833 4232 4264 2801 Size: 32 1399 32 6335 Fragments arranged by size: 6335 1399 32 32 AlwNI CAG_nnn'CTG Cuts at: 2161 2314 2924 3471 5541 7113 2161 Size: 153 610 547 2070 1572 2846 Fragments arranged by size: 2846 2070 1572 610 547 153 ApaI G_GGCC'C Cuts at: 2211 2444 2211 Size: 233 7565 ApaLI G'TGCA_C Cuts at: 5855 7011 5855 Size: 1156 6642 ApoI r'AATT_y Cuts at: 1493 2963 3351 3603 3704 3956 4107 5107 5333 Size: 1470 388 252 101 252 151 1000 226 Cuts at: 5333 5485 7594 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1470 1000 388 252 252 226 152 151 101 AseI AT'TA_AT Cuts at: 160 1032 6352 160 Size: 872 5320 1606 Fragments arranged by size: 5320 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7798 AvaI C'yCGr_G Cuts at: 1870 1943 2437 2635 2650 5007 5123 1870 Size: 73 494 198 15 2357 116 4545 Fragments arranged by size: 4545 2357 494 198 116 73 15 BamHI G'GATC_C Cuts at: 4668 5128 5495 4668 Size: 460 367 6971 Fragments arranged by size: 6971 460 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2646 5119 6574 618 Size: 325 217 627 859 2473 1455 1842 Fragments arranged by size: 2473 1842 1455 859 627 325 217 BanII G_rGCy'C Cuts at: 1391 2211 2337 2444 2456 2485 3057 3315 4287 Size: 820 126 107 12 29 572 258 972 Cuts at: 4287 4892 5117 1391 Size: 605 225 4072 Fragments arranged by size: 4072 972 820 605 572 258 225 126 107 29 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2043 2193 3428 4791 4869 5049 928 Size: 971 144 150 1235 1363 78 180 3677 Fragments arranged by size: 3677 1363 1235 971 180 150 144 78 BbvCI CC'TCA_GC Cuts at: 2255 2255 Size: 7798 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2591 2625 5989 6023 1903 Size: 34 50 34 570 34 3364 34 3678 Fragments arranged by size: 3678 3364 570 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5690 6900 5690 Size: 1210 6588 BclI T'GATC_A Cuts at: 1548 3757 1548 Size: 2209 5589 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4305 6409 136 Size: 107 122 71 631 605 2633 2104 1525 Fragments arranged by size: 2633 2104 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7793 7793 Size: 7798 BlpI GC'TnA_GC Cuts at: 2901 5215 2901 Size: 2314 5484 Bme1580I G_kGCm'C Cuts at: 1790 2211 2444 2651 5859 7015 1790 Size: 421 233 207 3208 1156 2573 Fragments arranged by size: 3208 2573 1156 421 233 207 BmgBI CAC'GTC Cuts at: 1318 3385 1318 Size: 2067 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2041 5529 6487 Size: 416 53 83 399 24 624 3488 958 Cuts at: 6487 442 Size: 1753 Fragments arranged by size: 3488 1753 958 624 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7798 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2960 2960 Size: 7798 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1995 2097 2414 2449 2573 2678 2816 2930 Size: 756 102 317 35 124 105 138 114 Cuts at: 2930 3633 4380 4876 4892 6458 1239 Size: 703 747 496 16 1566 2579 Fragments arranged by size: 2579 1566 756 747 703 496 317 138 124 114 105 102 35 16 Bpu10I CC'TnA_GC Cuts at: 2040 2255 2315 3004 3407 3748 2040 Size: 215 60 689 403 341 6090 Fragments arranged by size: 6090 689 403 341 215 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3513 5923 6788 7086 3513 Size: 2410 865 298 4225 Fragments arranged by size: 4225 2410 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2649 2693 3128 3765 4678 6461 882 Size: 617 1150 44 435 637 913 1783 2219 Fragments arranged by size: 2219 1783 1150 913 637 617 435 44 BsaAI yAC'GTr Cuts at: 493 1204 2579 4556 5589 493 Size: 711 1375 1977 1033 2702 Fragments arranged by size: 2702 1977 1375 1033 711 BsaBI GATnn'nnATC Cuts at: 3913 7792 3913 Size: 3879 3919 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3658 5605 5987 6622 Size: 53 83 186 936 2125 1947 382 635 Cuts at: 6622 275 Size: 1451 Fragments arranged by size: 2125 1947 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3570 6231 6629 6903 7050 7461 3570 Size: 2661 398 274 147 411 3907 Fragments arranged by size: 3907 2661 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2255 2285 2646 2676 1178 Size: 30 165 30 852 30 361 30 6300 Fragments arranged by size: 6300 852 361 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 2411 3962 4295 4325 1186 Size: 1204 21 1551 333 30 4659 Fragments arranged by size: 4659 1551 1204 333 30 21 BseYI C'CCAG_C Cuts at: 2591 2688 7001 7497 2591 Size: 97 4313 496 2892 Fragments arranged by size: 4313 2892 496 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 2369 2478 3484 3662 3829 4399 Size: 573 402 231 109 1006 178 167 570 Cuts at: 4399 4776 1163 Size: 377 4185 Fragments arranged by size: 4185 1006 573 570 402 377 231 178 167 109 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5248 6009 7037 1540 Size: 275 186 3247 761 1028 2301 Fragments arranged by size: 3247 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2337 4027 4892 5859 5944 7015 2337 Size: 1690 865 967 85 1071 3120 Fragments arranged by size: 3120 1690 1071 967 865 85 BsmI GAATG_Cn' Cuts at: 5309 5402 5309 Size: 93 7705 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2337 2444 2456 2485 2651 3057 Size: 399 421 126 107 12 29 166 406 Cuts at: 3057 3315 4027 4287 4892 5117 5859 5944 7015 Size: 258 712 260 605 225 742 85 1071 Cuts at: 7015 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 712 605 421 406 399 260 258 225 166 126 107 85 29 12 BspEI T'CCGG_A Cuts at: 7461 7461 Size: 7798 BspHI T'CATG_A Cuts at: 5687 7417 5687 Size: 1730 6068 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2007 2851 5153 844 Size: 1163 844 2302 3489 Fragments arranged by size: 3489 2302 1163 844 BsrBI CCG'CTC Cuts at: 1394 1823 5685 1394 Size: 429 3862 3507 Fragments arranged by size: 3862 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6293 6467 65 Size: 1183 5045 174 1396 Fragments arranged by size: 5045 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2226 6442 6629 1700 Size: 110 416 4216 187 2869 Fragments arranged by size: 4216 2869 416 187 110 BsrGI T'GTAC_A Cuts at: 96 2778 96 Size: 2682 5116 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7798 BssSI C'ACGA_G Cuts at: 5858 6870 5858 Size: 1012 6786 BstAPI GCAn_nnn'nTGC Cuts at: 794 3807 794 Size: 3013 4785 BstBI TT'CG_AA Cuts at: 5060 6602 5060 Size: 1542 6256 BstEII G'GTnAC_C Cuts at: 1184 2725 1184 Size: 1541 6257 BstXI CCAn_nnnn'nTGG Cuts at: 2019 2019 Size: 7798 BstYI r'GATC_y Cuts at: 1972 2283 4668 5128 5495 5880 5897 7349 7435 Size: 311 2385 460 367 385 17 1452 86 Cuts at: 7435 7447 7793 1972 Size: 12 346 1977 Fragments arranged by size: 2385 1977 1452 460 385 367 346 311 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2907 4215 2907 Size: 1308 6490 BtgI C'CryG_G Cuts at: 513 1065 2876 513 Size: 552 1811 5435 Fragments arranged by size: 5435 1811 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7059 Fragments arranged by size: 7059 683 56 BtsI GCAGTG_nn' Cuts at: 3472 4064 5314 6108 6128 3472 Size: 592 1250 794 20 5142 Fragments arranged by size: 5142 1250 794 592 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7798 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7763 DraI TTT'AAA Cuts at: 3544 5102 3544 Size: 1558 6240 DraIII CAC_nnn'GTG Cuts at: 3445 3445 Size: 7798 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6805 809 Size: 507 5489 1802 Fragments arranged by size: 5489 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2152 5245 6134 Size: 54 1425 50 275 18 322 3093 889 Cuts at: 6134 7763 8 Size: 1629 43 Fragments arranged by size: 3093 1629 1425 889 322 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5245 1537 Size: 275 3433 4090 Fragments arranged by size: 4090 3433 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2088 2180 3071 3126 4449 4842 5728 1353 Size: 735 92 891 55 1323 393 886 3423 Fragments arranged by size: 3423 1323 891 886 735 393 92 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2156 6385 6759 6905 387 Size: 1064 138 567 4229 374 146 1280 Fragments arranged by size: 4229 1280 1064 567 374 146 138 EcoICRI GAG'CTC Cuts at: 2335 4890 2335 Size: 2555 5243 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 2057 2097 2161 2187 2414 2449 Size: 393 363 62 40 64 26 227 35 Cuts at: 2449 2573 2678 2816 2930 3189 3250 3319 3385 Size: 124 105 138 114 259 61 69 66 Cuts at: 3385 3633 3973 4380 4384 4876 4892 5032 5861 Size: 248 340 407 4 492 16 140 829 Cuts at: 5861 6458 7245 7513 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 829 787 597 492 407 393 363 340 268 259 248 227 140 138 124 114 105 69 66 64 62 61 40 35 26 16 4 EcoO109I rG'GnC_Cy Cuts at: 2207 2208 2410 2434 2441 2676 2881 5016 2207 Size: 1 202 24 7 235 205 2135 4989 Fragments arranged by size: 4989 2135 235 205 202 24 7 1 EcoRI G'AATT_C Cuts at: 3351 4107 5107 5485 3351 Size: 756 1000 378 5664 Fragments arranged by size: 5664 1000 756 378 FspI TGC'GCA Cuts at: 6304 6304 Size: 7798 HaeII r_GCGC'y Cuts at: 1678 4606 6945 1678 Size: 2928 2339 2531 Fragments arranged by size: 2928 2531 2339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 2408 2440 3472 Size: 32 135 32 683 32 121 32 1032 Cuts at: 3472 3504 4399 4426 4431 4458 6439 6471 6513 Size: 32 895 27 5 27 1981 32 42 Cuts at: 6513 6545 1373 Size: 32 2626 Fragments arranged by size: 2626 1981 1032 895 683 135 121 42 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 4096 4657 5142 5388 669 Size: 988 281 2158 561 485 246 3079 Fragments arranged by size: 3079 2158 988 561 485 281 246 HindIII A'AGCT_T Cuts at: 748 3648 4875 748 Size: 2900 1227 3671 Fragments arranged by size: 3671 2900 1227 HpaI GTT'AAC Cuts at: 4657 5388 4657 Size: 731 7067 KpnI G_GTAC'C Cuts at: 5123 5123 Size: 7798 MfeI C'AATT_G Cuts at: 5397 5397 Size: 7798 MluI A'CGCG_T Cuts at: 7674 7674 Size: 7798 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2841 3606 3679 6912 7096 1305 Size: 1536 765 73 3233 184 2007 Fragments arranged by size: 3233 2007 1536 765 184 73 MscI TGG'CCA Cuts at: 10 64 1489 1832 2154 10 Size: 54 1425 343 322 5654 Fragments arranged by size: 5654 1425 343 322 54 MslI CAynn'nnrTG Cuts at: 518 1166 2500 3019 3252 3285 3423 3460 3544 Size: 648 1334 519 233 33 138 37 84 Cuts at: 3544 3614 4048 4116 4375 4470 5756 6115 6274 Size: 70 434 68 259 95 1286 359 159 Cuts at: 6274 518 Size: 2042 Fragments arranged by size: 2042 1334 1286 648 519 434 359 259 233 159 138 95 84 70 68 37 33 MspA1I CmG'CkG Cuts at: 1606 4031 5214 5891 7039 7284 1606 Size: 2425 1183 677 1148 245 2120 Fragments arranged by size: 2425 2120 1183 1148 677 245 NaeI GCC'GGC Cuts at: 2228 2228 Size: 7798 NcoI C'CATG_G Cuts at: 513 1065 2876 513 Size: 552 1811 5435 Fragments arranged by size: 5435 1811 552 NdeI CA'TA_TG Cuts at: 387 3835 3933 4949 5076 387 Size: 3448 98 1016 127 3109 Fragments arranged by size: 3448 3109 1016 127 98 NgoMIV G'CCGG_C Cuts at: 2226 2226 Size: 7798 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7798 NotI GC'GGCC_GC Cuts at: 5245 5245 Size: 7798 NspI r_CATG'y Cuts at: 1132 1229 2027 2893 3206 4199 5156 6701 1132 Size: 97 798 866 313 993 957 1545 2229 Fragments arranged by size: 2229 1545 993 957 866 798 313 97 PasI CC'CwG_GG Cuts at: 1348 4018 1348 Size: 2670 5128 PciI A'CATG_T Cuts at: 3202 6697 3202 Size: 3495 4303 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7798 PfoI T'CCnGG_A Cuts at: 2232 2426 2694 4908 2232 Size: 194 268 2214 5122 Fragments arranged by size: 5122 2214 268 194 PmeI GTTT'AAAC Cuts at: 5102 5102 Size: 7798 PmlI CAC'GTG Cuts at: 2579 2579 Size: 7798 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5867 5899 7380 7412 5867 Size: 32 1481 32 6253 Fragments arranged by size: 6253 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2410 2434 2676 2410 Size: 24 242 7532 Fragments arranged by size: 7532 242 24 PshAI GACnn'nnGTC Cuts at: 1613 3661 4008 1613 Size: 2048 347 5403 Fragments arranged by size: 5403 2048 347 PsiI TTA'TAA Cuts at: 3627 5368 3627 Size: 1741 6057 PspOMI G'GGCC_C Cuts at: 2207 2440 2207 Size: 233 7565 PstI C_TGCA'G Cuts at: 830 2406 3803 4010 5150 830 Size: 1576 1397 207 1140 3478 Fragments arranged by size: 3478 1576 1397 1140 207 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7798 SacI G_AGCT'C Cuts at: 2337 4892 2337 Size: 2555 5243 SalI G'TCGA_C Cuts at: 1936 5140 1936 Size: 3204 4594 SanDI GG'GwC_CC Cuts at: 2434 2434 Size: 7798 SapI GCTCTTCn'nnn_ Cuts at: 2180 3071 2180 Size: 891 6907 SbfI CC_TGCA'GG Cuts at: 5150 5150 Size: 7798 ScaI AGT'ACT Cuts at: 6046 7758 6046 Size: 1712 6086 SexAI A'CCwGG_T Cuts at: 2658 2672 2658 Size: 14 7784 SfcI C'TryA_G Cuts at: 826 1045 2402 2931 3742 3799 4006 4162 4677 Size: 219 1357 529 811 57 207 156 515 Cuts at: 4677 5146 6281 6962 7153 826 Size: 469 1135 681 191 1471 Fragments arranged by size: 1471 1357 1135 811 681 529 515 469 219 207 191 156 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7798 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7798 SmaI CCC'GGG Cuts at: 1872 2439 2652 5125 1872 Size: 567 213 2473 4545 Fragments arranged by size: 4545 2473 567 213 SmlI C'TyrA_G Cuts at: 820 1017 1943 3528 5007 5902 6803 7065 820 Size: 197 926 1585 1479 895 901 262 1553 Fragments arranged by size: 1585 1553 1479 926 901 895 262 197 SnaBI TAC'GTA Cuts at: 493 5589 493 Size: 5096 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7798 SphI G_CATG'C Cuts at: 1132 2027 4199 5156 1132 Size: 895 2172 957 3774 Fragments arranged by size: 3774 2172 957 895 SspI AAT'ATT Cuts at: 5 52 5722 5 Size: 47 5670 2081 Fragments arranged by size: 5670 2081 47 StuI AGG'CCT Cuts at: 1517 2260 4313 1517 Size: 743 2053 5002 Fragments arranged by size: 5002 2053 743 StyI C'CwwG_G Cuts at: 513 1065 2274 2876 5237 513 Size: 552 1209 602 2361 3074 Fragments arranged by size: 3074 2361 1209 602 552 SwaI ATTT'AAAT Cuts at: 3544 3544 Size: 7798 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2335 3878 5825 5842 5995 6180 6625 Size: 1255 755 1543 1947 17 153 185 445 Cuts at: 6625 325 Size: 1498 Fragments arranged by size: 1947 1543 1498 1255 755 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2778 6044 7756 Size: 274 80 33 51 1443 801 3266 1712 Cuts at: 7756 96 Size: 138 Fragments arranged by size: 3266 1712 1443 801 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2568 2600 7758 7790 2568 Size: 32 5158 32 2576 Fragments arranged by size: 5158 2576 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7798 XbaI T'CTAG_A Cuts at: 5134 5134 Size: 7798 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2463 2463 Size: 7798 XhoI C'TCGA_G Cuts at: 1943 5007 1943 Size: 3064 4734 XmaI C'CCGG_G Cuts at: 1870 2437 2650 5123 1870 Size: 567 213 2473 4545 Fragments arranged by size: 4545 2473 567 213 XmnI GAAnn'nnTTC Cuts at: 3066 3274 3566 3650 3818 4397 4715 5927 3066 Size: 208 292 84 168 579 318 1212 4937 Fragments arranged by size: 4937 1212 579 318 292 208 168 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5606 276 Size: 53 83 186 936 4072 2468 Fragments arranged by size: 4072 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SfiI SfoI SrfI