(Circular) (Six-base) MAPSORT of: pFN21AB9213.seq Check: 758 from: 1 to: 7222 pFN21AB9213 7222 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5032 278 Size: 53 83 186 936 3496 2468 Fragments arranged by size: 3496 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4565 1937 Size: 2628 4594 Acc65I G'GTAC_C Cuts at: 4543 4543 Size: 7222 AclI AA'CG_TT Cuts at: 1563 4127 5349 5722 1563 Size: 2564 1222 373 3063 Fragments arranged by size: 3063 2564 1222 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2009 2088 2757 3878 3887 4406 5285 6669 Size: 377 79 669 1121 9 519 879 1384 Cuts at: 6669 6937 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1121 879 669 519 377 268 79 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7025 AflIII A'CryG_T Cuts at: 1203 6121 7098 1203 Size: 4918 977 1327 Fragments arranged by size: 4918 1327 977 AgeI A'CCGG_T Cuts at: 6053 6053 Size: 7222 AhdI GACnn_n'nnGTC Cuts at: 5951 5951 Size: 7222 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7222 AlwNI CAG_nnn'CTG Cuts at: 2084 2582 2614 4965 6537 2084 Size: 498 32 2351 1572 2769 Fragments arranged by size: 2769 2351 1572 498 32 ApaI G_GGCC'C Cuts at: 3146 3146 Size: 7222 ApaLI G'TGCA_C Cuts at: 3570 5279 6435 3570 Size: 1709 1156 4357 Fragments arranged by size: 4357 1709 1156 ApoI r'AATT_y Cuts at: 1493 2393 4531 4757 4909 7018 1493 Size: 900 2138 226 152 2109 1697 Fragments arranged by size: 2138 2109 1697 900 226 152 AseI AT'TA_AT Cuts at: 160 1032 5776 160 Size: 872 4744 1606 Fragments arranged by size: 4744 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7222 AvaI C'yCGr_G Cuts at: 1870 1943 4547 1870 Size: 73 2604 4545 Fragments arranged by size: 4545 2604 73 BamHI G'GATC_C Cuts at: 4552 4919 4552 Size: 367 6855 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2332 2987 4543 5998 618 Size: 325 217 627 545 655 1556 1455 1842 Fragments arranged by size: 1842 1556 1455 655 627 545 325 217 BanII G_rGCy'C Cuts at: 1391 2493 3146 3219 4541 1391 Size: 1102 653 73 1322 4072 Fragments arranged by size: 4072 1322 1102 653 73 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2829 3649 928 Size: 971 930 820 4501 Fragments arranged by size: 4501 971 930 820 BbvCI CC'TCA_GC Cuts at: 2835 2835 Size: 7222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5413 5447 1903 Size: 34 3476 34 3678 Fragments arranged by size: 3678 3476 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2758 5114 6324 2758 Size: 2356 1210 3656 Fragments arranged by size: 3656 2356 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7222 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5833 136 Size: 107 122 71 631 605 4161 1525 Fragments arranged by size: 4161 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2776 3070 3775 7217 2776 Size: 294 705 3442 2781 Fragments arranged by size: 3442 2781 705 294 BlpI GC'TnA_GC Cuts at: 3597 4639 3597 Size: 1042 6180 Bme1580I G_kGCm'C Cuts at: 1790 2528 3146 3153 3574 5283 6439 1790 Size: 738 618 7 421 1709 1156 2573 Fragments arranged by size: 2573 1709 1156 738 618 421 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7222 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3147 4953 5911 Size: 416 53 83 399 24 1730 1806 958 Cuts at: 5911 442 Size: 1753 Fragments arranged by size: 1806 1753 1730 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7222 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3072 4157 3072 Size: 1085 6137 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3067 5882 1239 Size: 1828 2815 2579 Fragments arranged by size: 2815 2579 1828 Bpu10I CC'TnA_GC Cuts at: 2653 2835 4288 2653 Size: 182 1453 5587 Fragments arranged by size: 5587 1453 182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2245 3481 5347 6212 6510 2245 Size: 1236 1866 865 298 2957 Fragments arranged by size: 2957 1866 1236 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2507 3296 5885 882 Size: 617 1008 789 2589 2219 Fragments arranged by size: 2589 2219 1008 789 617 BsaAI yAC'GTr Cuts at: 493 1204 3275 5013 493 Size: 711 2071 1738 2702 Fragments arranged by size: 2702 2071 1738 711 BsaBI GATnn'nnATC Cuts at: 7216 7216 Size: 7222 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2141 5029 5411 6046 Size: 53 83 186 936 608 2888 382 635 Cuts at: 6046 275 Size: 1451 Fragments arranged by size: 2888 1451 936 635 608 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5655 6053 6327 6474 6885 5655 Size: 398 274 147 411 5992 Fragments arranged by size: 5992 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6997 Fragments arranged by size: 6997 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3045 1186 Size: 1859 5363 BseYI C'CCAG_C Cuts at: 3110 6425 6921 3110 Size: 3315 496 3411 Fragments arranged by size: 3411 3315 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3252 4062 4091 1163 Size: 573 1516 810 29 4294 Fragments arranged by size: 4294 1516 810 573 29 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4672 5433 6461 1540 Size: 275 186 2671 761 1028 2301 Fragments arranged by size: 2671 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2493 3574 4505 5283 5368 6439 2493 Size: 1081 931 778 85 1071 3276 Fragments arranged by size: 3276 1081 1071 931 778 85 BsmI GAATG_Cn' Cuts at: 3722 4733 4826 3722 Size: 1011 93 6118 Fragments arranged by size: 6118 1011 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3662 3662 Size: 7222 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2493 2528 3146 3153 3219 3574 4505 Size: 399 703 35 618 7 66 355 931 Cuts at: 4505 4541 5283 5368 6439 1391 Size: 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 931 742 703 618 399 355 85 66 36 35 7 BspEI T'CCGG_A Cuts at: 6885 6885 Size: 7222 BspHI T'CATG_A Cuts at: 5111 6841 5111 Size: 1730 5492 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2043 4577 844 Size: 1199 2534 3489 Fragments arranged by size: 3489 2534 1199 BsrBI CCG'CTC Cuts at: 1394 1823 2315 5109 1394 Size: 429 492 2794 3507 Fragments arranged by size: 3507 2794 492 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2566 4023 5717 5891 65 Size: 1183 1318 1457 1694 174 1396 Fragments arranged by size: 1694 1457 1396 1318 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2364 5866 6053 1700 Size: 110 554 3502 187 2869 Fragments arranged by size: 3502 2869 554 187 110 BsrGI T'GTAC_A Cuts at: 96 2746 96 Size: 2650 4572 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7222 BssSI C'ACGA_G Cuts at: 3567 3734 5282 6294 3567 Size: 167 1548 1012 4495 Fragments arranged by size: 4495 1548 1012 167 BstAPI GCAn_nnn'nTGC Cuts at: 794 3275 4270 794 Size: 2481 995 3746 Fragments arranged by size: 3746 2481 995 BstBI TT'CG_AA Cuts at: 6026 6026 Size: 7222 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7222 BstYI r'GATC_y Cuts at: 1972 2776 3070 3775 4552 4919 5304 5321 6773 Size: 804 294 705 777 367 385 17 1452 Cuts at: 6773 6859 6871 7217 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 804 777 705 385 367 346 294 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3525 3525 Size: 7222 BtgI C'CryG_G Cuts at: 513 1065 2004 3171 4245 513 Size: 552 939 1167 1074 3490 Fragments arranged by size: 3490 1167 1074 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2253 2326 3420 4455 487 Size: 683 56 1027 73 1094 1035 3254 Fragments arranged by size: 3254 1094 1035 1027 683 73 56 BtsI GCAGTG_nn' Cuts at: 3655 3704 4738 5532 5552 3655 Size: 49 1034 794 20 5325 Fragments arranged by size: 5325 1034 794 49 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7222 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7187 DraI TTT'AAA Cuts at: 2668 3804 4526 2668 Size: 1136 722 5364 Fragments arranged by size: 5364 1136 722 DraIII CAC_nnn'GTG Cuts at: 3275 3275 Size: 7222 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4442 6229 809 Size: 507 3126 1787 1802 Fragments arranged by size: 3126 1802 1787 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2286 3174 4669 Size: 54 1425 50 275 18 456 888 1495 Cuts at: 4669 5558 7187 8 Size: 889 1629 43 Fragments arranged by size: 1629 1495 1425 889 888 456 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4669 1537 Size: 275 2857 4090 Fragments arranged by size: 4090 2857 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2685 3063 3294 3438 3877 4425 5152 1353 Size: 1332 378 231 144 439 548 727 3423 Fragments arranged by size: 3423 1332 727 548 439 378 231 144 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5809 6183 6329 387 Size: 1064 138 435 3785 374 146 1280 Fragments arranged by size: 3785 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2491 2491 Size: 7222 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2009 2088 2757 3067 3878 3887 4406 Size: 393 377 79 669 310 811 9 519 Cuts at: 4406 5285 5882 6669 6937 1239 Size: 879 597 787 268 1524 Fragments arranged by size: 1524 879 811 787 669 597 519 393 377 310 268 79 9 EcoNI CCTnn'n_nnAGG Cuts at: 2039 2039 Size: 7222 EcoO109I rG'GnC_Cy Cuts at: 2032 2618 3142 3143 2032 Size: 586 524 1 6111 Fragments arranged by size: 6111 586 524 1 EcoRI G'AATT_C Cuts at: 4531 4909 4531 Size: 378 6844 FspI TGC'GCA Cuts at: 5728 5728 Size: 7222 HaeII r_GCGC'y Cuts at: 1678 6369 1678 Size: 4691 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2105 2137 2710 Size: 32 135 32 488 32 13 32 573 Cuts at: 2710 2742 3119 3151 3193 3225 5863 5895 5937 Size: 32 377 32 42 32 2638 32 42 Cuts at: 5937 5969 1373 Size: 32 2626 Fragments arranged by size: 2638 2626 573 488 377 135 42 42 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 2139 3583 4566 4812 669 Size: 988 281 201 1444 983 246 3079 Fragments arranged by size: 3079 1444 988 983 281 246 201 HindIII A'AGCT_T Cuts at: 748 3860 748 Size: 3112 4110 HpaI GTT'AAC Cuts at: 4812 4812 Size: 7222 KpnI G_GTAC'C Cuts at: 4547 4547 Size: 7222 MfeI C'AATT_G Cuts at: 3761 4821 3761 Size: 1060 6162 MluI A'CGCG_T Cuts at: 7098 7098 Size: 7222 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2460 4046 6336 6520 1305 Size: 1155 1586 2290 184 2007 Fragments arranged by size: 2290 2007 1586 1155 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3176 10 Size: 54 1425 343 1344 4056 Fragments arranged by size: 4056 1425 1344 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3170 3225 3846 5180 5539 5698 518 Size: 648 2004 55 621 1334 359 159 2042 Fragments arranged by size: 2042 2004 1334 648 621 359 159 55 MspA1I CmG'CkG Cuts at: 1606 3266 3770 3967 4383 4638 5315 6463 6708 Size: 1660 504 197 416 255 677 1148 245 Cuts at: 6708 1606 Size: 2120 Fragments arranged by size: 2120 1660 1148 677 504 416 255 245 197 NcoI C'CATG_G Cuts at: 513 1065 2004 3171 4245 513 Size: 552 939 1167 1074 3490 Fragments arranged by size: 3490 1167 1074 939 552 NdeI CA'TA_TG Cuts at: 387 2380 387 Size: 1993 5229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7222 NotI GC'GGCC_GC Cuts at: 4669 4669 Size: 7222 NspI r_CATG'y Cuts at: 1132 1229 3697 4580 6125 1132 Size: 97 2468 883 1545 2229 Fragments arranged by size: 2468 2229 1545 883 97 PasI CC'CwG_GG Cuts at: 1348 2146 1348 Size: 798 6424 PciI A'CATG_T Cuts at: 6121 6121 Size: 7222 PflMI CCAn_nnn'nTGG Cuts at: 1108 2984 1108 Size: 1876 5346 PfoI T'CCnGG_A Cuts at: 2843 2843 Size: 7222 PmeI GTTT'AAAC Cuts at: 4526 4526 Size: 7222 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5291 5323 6804 6836 5291 Size: 32 1481 32 5677 Fragments arranged by size: 5677 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2618 2032 Size: 586 6636 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7222 PsiI TTA'TAA Cuts at: 4792 4792 Size: 7222 PspOMI G'GGCC_C Cuts at: 3142 3142 Size: 7222 PstI C_TGCA'G Cuts at: 830 2040 3775 4574 830 Size: 1210 1735 799 3478 Fragments arranged by size: 3478 1735 1210 799 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7222 PvuII CAG'CTG Cuts at: 3770 3967 4383 3770 Size: 197 416 6609 Fragments arranged by size: 6609 416 197 SacI G_AGCT'C Cuts at: 2493 2493 Size: 7222 SalI G'TCGA_C Cuts at: 1936 4564 1936 Size: 2628 4594 SapI GCTCTTCn'nnn_ Cuts at: 4425 4425 Size: 7222 SbfI CC_TGCA'GG Cuts at: 2040 4574 2040 Size: 2534 4688 ScaI AGT'ACT Cuts at: 5470 7182 5470 Size: 1712 5510 SexAI A'CCwGG_T Cuts at: 2950 2950 Size: 7222 SfcI C'TryA_G Cuts at: 826 1045 2036 2071 3771 4570 5705 6386 6577 Size: 219 991 35 1700 799 1135 681 191 Cuts at: 6577 826 Size: 1471 Fragments arranged by size: 1700 1471 1135 991 799 681 219 191 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7222 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7222 SmaI CCC'GGG Cuts at: 1872 4549 1872 Size: 2677 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2224 3460 5326 6227 6489 820 Size: 197 926 281 1236 1866 901 262 1553 Fragments arranged by size: 1866 1553 1236 926 901 281 262 197 SnaBI TAC'GTA Cuts at: 493 5013 493 Size: 4520 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7222 SphI G_CATG'C Cuts at: 1132 4580 1132 Size: 3448 3774 SspI AAT'ATT Cuts at: 5 52 5146 5 Size: 47 5094 2081 Fragments arranged by size: 5094 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7222 StyI C'CwwG_G Cuts at: 513 1065 2004 3171 4245 4661 513 Size: 552 939 1167 1074 416 3074 Fragments arranged by size: 3074 1167 1074 939 552 416 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5249 5266 5419 5604 6049 325 Size: 1255 3669 17 153 185 445 1498 Fragments arranged by size: 3669 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2746 2894 3508 Size: 274 80 33 51 1443 769 148 614 Cuts at: 3508 5468 7180 96 Size: 1960 1712 138 Fragments arranged by size: 1960 1712 1443 769 614 274 148 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2046 2078 7182 7214 2046 Size: 32 5104 32 2054 Fragments arranged by size: 5104 2054 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2117 1316 Size: 801 6421 XbaI T'CTAG_A Cuts at: 3073 3211 3979 4558 3073 Size: 138 768 579 5737 Fragments arranged by size: 5737 768 579 138 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2803 3187 2803 Size: 384 6838 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7222 XmaI C'CCGG_G Cuts at: 1870 4547 1870 Size: 2677 4545 XmnI GAAnn'nnTTC Cuts at: 5351 5351 Size: 7222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5030 276 Size: 53 83 186 936 3496 2468 Fragments arranged by size: 3496 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI