Restriction map for
pFN21AB9128
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccII AccIII AcyI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AspI
AvaI AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BclI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI BstEII BstPI
BstUI Bsu36I CelII Cfr10I ClaI
CpoI DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT14I FauI FbaI
FseI HaeII Hin1I HincII HindII
HpaI KasI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1188 GGTACC G^GTACC
AccI 1473 GTATAC GT^[AC][GT]AC
AclI 114 AACGTT AA^CGTT
AflII 1069 CTTAAG C^TTAAG
AlwNI 529 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 448 ATTAAT AT^TAAT
AsnI 448 ATTAAT AT^TAAT
Asp718I 1188 GGTACC G^GTACC
AspHI 1631 GTGCTC G[AT]GC[AT]^C
AviII 1753 TGCGCA TGC^GCA
BcnI 17 CCCGG CC^[GC]GG
BfrI 1069 CTTAAG C^TTAAG
BsaAI 1423 CACGTA [CT]AC^GT[GA]
BsaI 2676 GAGACC GGTCTC(1/5)
BsiHKAI 1631 GTGCTC G[AT]GC[AT]^C
BspHI 2672 TCATGA T^CATGA
BspMI 2604 ACCTGC ACCTGC(4/8)
BsrBI 1496 GAGCGG CCGCTC(-3/-3)
Bst1107I 1473 GTATAC GTA^TAC
BstBI 1112 TTCGAA TT^CGAA
BstYI 1891 GGATCT [GA]^GATC[CT]
BstZ17I 1473 GTATAC GTA^TAC
CfoI 1754 GCGC GCG^C
DrdI 407 GACTAGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 873 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1591 GAATTC G^AATTC
FspI 1753 TGCGCA TGC^GCA
HapII 18 CCGG C^CGG
HgaI 2536 GCGTC GACGC(5/10)
HhaI 1754 GCGC GCG^C
HinP1I 1754 GCGC G^CGC
HpaII 18 CCGG C^CGG
KpnI 1188 GGTACC GGTAC^C
MfeI 2531 CAATTG C^AATTG
MflI 1891 GGATCT [GA]^GATC[CT]
MspA1I 332 CAGCTG C[AC]G^C[GT]G
MspI 18 CCGG C^CGG
MunI 2531 CAATTG C^AATTG
NciI 17 CCCGG CC^[GC]GG
NspV 1112 TTCGAA TT^CGAA
PflMI 1179 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 462 GACTC GAGTC(4/5)
PshAI 2678 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 448 ATTAAT AT^TAAT
Psp1406I 114 AACGTT AA^CGTT
PvuII 332 CAGCTG CAG^CTG
RcaI 2672 TCATGA T^CATGA
ScaI 2476 AGTACT AGT^ACT
SfuI 1112 TTCGAA TT^CGAA
Van91I 1179 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2504 CCAGCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1891 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 1891 GGATC GGATC(4/5)
2400 GGATC
BanI 1188 GGTACC G^G[CT][GA]CC
1341 GGTGCC
BciVI 836 GGATAC GTATCC(6/5)
1255 GGATAC
BmyI 1631 GTGCTC G[AGT]GC[ACT]^C
2044 GGGCAC
BpmI 1380 CTCCAG CTGGAG(16/14)
1640 CTCCAG
BsmI 45 GAATGC GAATGC(1/-1)
1750 GAATGC
Bsp1286I 1631 GTGCTC G[AGT]GC[ACT]^C
2044 GGGCAC
BsrDI 782 GCAATG GCAATG(2/0)
1921 GCAATG
BstXI 1131 CCAGCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2504 CCAGCACTGTGG
DraI 1413 TTTAAA TTT^AAA
2191 TTTAAA
EarI 2435 CTCTTC CTCTTC(1/4)
1359 GAAGAG
EcoT22I 862 ATGCAT ATGCA^T
1957 ATGCAT
HindIII 733 AAGCTT A^AGCTT
1099 AAGCTT
Ksp632I 2435 CTCTTC CTCTTC(1/4)
1359 GAAGAG
MslI 748 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2508 CACTGTGGTG
NsiI 862 ATGCAT ATGCA^T
1957 ATGCAT
NspI 1164 GCATGT [GA]CATG^[CT]
1273 GCATGT
PpuMI 850 AGGACCT [GA]G^G[AT]CC[CT]
1653 GGGACCT
PstI 1033 CTGCAG CTGCA^G
2585 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AvaII BbsI BbvI BfaI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstAPI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI