(Circular) (Six-base) MAPSORT of: pFN21AB9128.seq Check: 4653 from: 1 to: 7411 pFN21AB9128 7411 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5221 278 Size: 53 83 186 936 3685 2468 Fragments arranged by size: 3685 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 4754 1937 Size: 1542 1275 4594 Fragments arranged by size: 4594 1542 1275 Acc65I G'GTAC_C Cuts at: 3193 4732 3193 Size: 1539 5872 AclI AA'CG_TT Cuts at: 1563 2120 5538 5911 1563 Size: 557 3418 373 3063 Fragments arranged by size: 3418 3063 557 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2412 2604 3962 4427 4623 5474 6858 7126 Size: 780 192 1358 465 196 851 1384 268 Cuts at: 7126 1632 Size: 1917 Fragments arranged by size: 1917 1384 1358 851 780 465 268 196 192 AflII C'TTAA_G Cuts at: 820 1017 3074 820 Size: 197 2057 5157 Fragments arranged by size: 5157 2057 197 AflIII A'CryG_T Cuts at: 1203 6310 7287 1203 Size: 5107 977 1327 Fragments arranged by size: 5107 1327 977 AgeI A'CCGG_T Cuts at: 6242 6242 Size: 7411 AhdI GACnn_n'nnGTC Cuts at: 6140 6140 Size: 7411 AleI CACnn'nnGTG Cuts at: 1166 4517 1166 Size: 3351 4060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3987 1184 Size: 2803 4608 AlwNI CAG_nnn'CTG Cuts at: 2539 5154 6726 2539 Size: 2615 1572 3224 Fragments arranged by size: 3224 2615 1572 ApaLI G'TGCA_C Cuts at: 5468 6624 5468 Size: 1156 6255 ApoI r'AATT_y Cuts at: 1493 2185 2286 2358 2591 2686 2989 3047 3208 Size: 692 101 72 233 95 303 58 161 Cuts at: 3208 3596 4720 4946 5098 7207 1493 Size: 388 1124 226 152 2109 1697 Fragments arranged by size: 2109 1697 1124 692 388 303 233 226 161 152 101 95 72 58 AseI AT'TA_AT Cuts at: 160 1032 2454 5965 160 Size: 872 1422 3511 1606 Fragments arranged by size: 3511 1606 1422 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7411 AvaI C'yCGr_G Cuts at: 1870 1943 4736 1870 Size: 73 2793 4545 Fragments arranged by size: 4545 2793 73 BamHI G'GATC_C Cuts at: 4741 5108 4741 Size: 367 7044 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3193 3346 4732 6187 618 Size: 325 217 627 1406 153 1386 1455 1842 Fragments arranged by size: 1842 1455 1406 1386 627 325 217 153 BanII G_rGCy'C Cuts at: 1391 4730 1391 Size: 3339 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2909 3095 3311 928 Size: 971 1010 186 216 5028 Fragments arranged by size: 5028 1010 971 216 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5602 5636 1903 Size: 34 3665 34 3678 Fragments arranged by size: 3678 3665 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2835 3254 5303 6513 2835 Size: 419 2049 1210 3733 Fragments arranged by size: 3733 2049 1210 419 BclI T'GATC_A Cuts at: 1548 1548 Size: 7411 BfrBI ATG'CAT Cuts at: 2869 3964 2869 Size: 1095 6316 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6022 136 Size: 107 122 71 631 605 4350 1525 Fragments arranged by size: 4350 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7406 7406 Size: 7411 BlpI GC'TnA_GC Cuts at: 4828 4828 Size: 7411 Bme1580I G_kGCm'C Cuts at: 1790 4053 5472 6628 1790 Size: 2263 1419 1156 2573 Fragments arranged by size: 2573 2263 1419 1156 BmgBI CAC'GTC Cuts at: 1318 4332 1318 Size: 3014 4397 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2707 4016 5142 Size: 416 53 83 399 24 1290 1309 1126 Cuts at: 5142 6100 442 Size: 958 1753 Fragments arranged by size: 1753 1309 1290 1126 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7411 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3370 3630 6071 1239 Size: 2131 260 2441 2579 Fragments arranged by size: 2579 2441 2131 260 Bpu10I CC'TnA_GC Cuts at: 3069 3069 Size: 7411 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2454 2628 3086 3637 5536 6401 6699 2454 Size: 174 458 551 1899 865 298 3166 Fragments arranged by size: 3166 1899 865 551 458 298 174 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4675 6074 882 Size: 617 3176 1399 2219 Fragments arranged by size: 3176 2219 1399 617 BsaAI yAC'GTr Cuts at: 493 1204 3430 5202 493 Size: 711 2226 1772 2702 Fragments arranged by size: 2702 2226 1772 711 BsaBI GATnn'nnATC Cuts at: 7405 7405 Size: 7411 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5218 5600 6235 275 Size: 53 83 186 936 3685 382 635 1451 Fragments arranged by size: 3685 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5844 6242 6516 6663 7074 5844 Size: 398 274 147 411 6181 Fragments arranged by size: 6181 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7186 Fragments arranged by size: 7186 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7411 BseYI C'CCAG_C Cuts at: 6614 7110 6614 Size: 496 6915 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6838 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4861 5622 6650 1540 Size: 275 186 2860 761 1028 2301 Fragments arranged by size: 2860 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3640 5472 5557 6628 3640 Size: 1832 85 1071 4423 Fragments arranged by size: 4423 1832 1071 85 BsmI GAATG_Cn' Cuts at: 2056 3761 4922 5015 2056 Size: 1705 1161 93 4452 Fragments arranged by size: 4452 1705 1161 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3640 4053 4730 5472 5557 6628 1391 Size: 399 1850 413 677 742 85 1071 2174 Fragments arranged by size: 2174 1850 1071 742 677 413 399 85 BspEI T'CCGG_A Cuts at: 7074 7074 Size: 7411 BspHI T'CATG_A Cuts at: 4677 5300 7030 4677 Size: 623 1730 5058 Fragments arranged by size: 5058 1730 623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4618 4766 844 Size: 3774 148 3489 Fragments arranged by size: 3774 3489 148 BsrBI CCG'CTC Cuts at: 1394 1823 3503 5298 1394 Size: 429 1680 1795 3507 Fragments arranged by size: 3507 1795 1680 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2794 3933 5906 6080 65 Size: 1183 1546 1139 1973 174 1396 Fragments arranged by size: 1973 1546 1396 1183 1139 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6055 6242 1700 Size: 110 4245 187 2869 Fragments arranged by size: 4245 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7411 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7411 BssSI C'ACGA_G Cuts at: 5471 6483 5471 Size: 1012 6399 BstAPI GCAn_nnn'nTGC Cuts at: 794 2300 2539 3923 794 Size: 1506 239 1384 4282 Fragments arranged by size: 4282 1506 1384 239 BstBI TT'CG_AA Cuts at: 3118 6215 3118 Size: 3097 4314 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7411 BstXI CCAn_nnnn'nTGG Cuts at: 3143 4516 3143 Size: 1373 6038 BstYI r'GATC_y Cuts at: 1972 3896 4741 5108 5493 5510 6962 7048 7060 Size: 1924 845 367 385 17 1452 86 12 Cuts at: 7060 7406 1972 Size: 346 1977 Fragments arranged by size: 1977 1924 1452 845 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3480 3480 Size: 7411 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5920 Fragments arranged by size: 5920 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6672 Fragments arranged by size: 6672 683 56 BtsI GCAGTG_nn' Cuts at: 3035 4927 5721 5741 3035 Size: 1892 794 20 4705 Fragments arranged by size: 4705 1892 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7411 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7376 DraI TTT'AAA Cuts at: 3420 4198 4715 3420 Size: 778 517 6116 Fragments arranged by size: 6116 778 517 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2418 6418 809 Size: 507 1102 4000 1802 Fragments arranged by size: 4000 1802 1102 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4858 5747 7376 Size: 54 1425 50 275 18 3028 889 1629 Cuts at: 7376 8 Size: 43 Fragments arranged by size: 3028 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4858 1537 Size: 275 3046 4090 Fragments arranged by size: 4090 3046 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3359 4446 5341 1353 Size: 2006 1087 895 3423 Fragments arranged by size: 3423 2006 1087 895 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5998 6372 6518 387 Size: 1064 138 4409 374 146 1280 Fragments arranged by size: 4409 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2412 2604 3370 3630 3962 4427 4623 Size: 393 780 192 766 260 332 465 196 Cuts at: 4623 5474 6071 6858 7126 1239 Size: 851 597 787 268 1524 Fragments arranged by size: 1524 851 787 780 766 597 465 393 332 268 260 196 192 EcoNI CCTnn'n_nnAGG Cuts at: 2882 2882 Size: 7411 EcoO109I rG'GnC_Cy Cuts at: 2856 3659 4671 2856 Size: 803 1012 5596 Fragments arranged by size: 5596 1012 803 EcoRI G'AATT_C Cuts at: 3596 4720 5098 3596 Size: 1124 378 5909 Fragments arranged by size: 5909 1124 378 FspI TGC'GCA Cuts at: 3760 5917 3760 Size: 2157 5254 FspAI rTGC'GCAy Cuts at: 3760 3760 Size: 7411 HaeII r_GCGC'y Cuts at: 1678 6558 1678 Size: 4880 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2258 2290 2626 2658 2687 Size: 32 135 32 686 32 336 32 29 Cuts at: 2687 2719 3304 3336 3499 3514 3531 3546 6052 Size: 32 585 32 163 15 17 15 2506 Cuts at: 6052 6084 6126 6158 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2506 686 585 336 163 135 42 32 32 32 32 32 32 32 32 29 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 4755 5001 669 Size: 988 281 2817 246 3079 Fragments arranged by size: 3079 2817 988 281 246 HindIII A'AGCT_T Cuts at: 748 2738 3104 748 Size: 1990 366 5055 Fragments arranged by size: 5055 1990 366 HpaI GTT'AAC Cuts at: 5001 5001 Size: 7411 KpnI G_GTAC'C Cuts at: 3197 4736 3197 Size: 1539 5872 MfeI C'AATT_G Cuts at: 4536 5010 4536 Size: 474 6937 MluI A'CGCG_T Cuts at: 7287 7287 Size: 7411 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3238 4187 4204 4671 6525 6709 1305 Size: 1933 949 17 467 1854 184 2007 Fragments arranged by size: 2007 1933 1854 949 467 184 17 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5589 Fragments arranged by size: 5589 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2757 4517 5369 5728 5887 518 Size: 648 1591 1760 852 359 159 2042 Fragments arranged by size: 2042 1760 1591 852 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2339 4827 5504 6652 6897 1606 Size: 733 2488 677 1148 245 2120 Fragments arranged by size: 2488 2120 1148 733 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5920 Fragments arranged by size: 5920 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7411 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7411 NotI GC'GGCC_GC Cuts at: 4858 4858 Size: 7411 NsiI A_TGCA'T Cuts at: 2871 3966 2871 Size: 1095 6316 NspI r_CATG'y Cuts at: 1132 1229 3173 3282 4769 6314 1132 Size: 97 1944 109 1487 1545 2229 Fragments arranged by size: 2229 1944 1545 1487 109 97 PasI CC'CwG_GG Cuts at: 1348 2066 1348 Size: 718 6693 PciI A'CATG_T Cuts at: 6310 6310 Size: 7411 PflMI CCAn_nnn'nTGG Cuts at: 1108 3190 1108 Size: 2082 5329 PfoI T'CCnGG_A Cuts at: 3669 3768 3669 Size: 99 7312 PmeI GTTT'AAAC Cuts at: 4715 4715 Size: 7411 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3450 3482 5480 5512 6993 7025 3450 Size: 32 1998 32 1481 32 3836 Fragments arranged by size: 3836 1998 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2856 3659 2856 Size: 803 6608 PshAI GACnn'nnGTC Cuts at: 1613 4687 1613 Size: 3074 4337 PsiI TTA'TAA Cuts at: 3017 4478 4981 3017 Size: 1461 503 5447 Fragments arranged by size: 5447 1461 503 PstI C_TGCA'G Cuts at: 830 3042 4594 4763 830 Size: 2212 1552 169 3478 Fragments arranged by size: 3478 2212 1552 169 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7411 PvuII CAG'CTG Cuts at: 2339 2339 Size: 7411 SalI G'TCGA_C Cuts at: 1936 4753 1936 Size: 2817 4594 SbfI CC_TGCA'GG Cuts at: 4763 4763 Size: 7411 ScaI AGT'ACT Cuts at: 4483 5659 7371 4483 Size: 1176 1712 4523 Fragments arranged by size: 4523 1712 1176 SfcI C'TryA_G Cuts at: 826 1045 2178 2637 2879 3038 4590 4759 5894 Size: 219 1133 459 242 159 1552 169 1135 Cuts at: 5894 6575 6766 826 Size: 681 191 1471 Fragments arranged by size: 1552 1471 1135 1133 681 459 242 219 191 169 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7411 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7411 SmaI CCC'GGG Cuts at: 1872 4738 1872 Size: 2866 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2469 2643 3074 3101 3616 5515 Size: 197 926 526 174 431 27 515 1899 Cuts at: 5515 6416 6678 820 Size: 901 262 1553 Fragments arranged by size: 1899 1553 926 901 526 515 431 262 197 174 27 SnaBI TAC'GTA Cuts at: 493 5202 493 Size: 4709 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7411 SphI G_CATG'C Cuts at: 1132 4769 1132 Size: 3637 3774 SspI AAT'ATT Cuts at: 5 52 3060 3707 4354 5335 5 Size: 47 3008 647 647 981 2081 Fragments arranged by size: 3008 2081 981 647 647 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7411 StyI C'CwwG_G Cuts at: 513 1065 2004 4850 513 Size: 552 939 2846 3074 Fragments arranged by size: 3074 2846 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5438 5455 5608 5793 6238 325 Size: 1255 3858 17 153 185 445 1498 Fragments arranged by size: 3858 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2473 4481 5657 Size: 274 80 33 51 1443 496 2008 1176 Cuts at: 5657 7369 96 Size: 1712 138 Fragments arranged by size: 2008 1712 1443 1176 496 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7371 7403 7371 Size: 32 7379 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7411 XbaI T'CTAG_A Cuts at: 4747 4747 Size: 7411 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4516 4516 Size: 7411 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7411 XmaI C'CCGG_G Cuts at: 1870 4736 1870 Size: 2866 4545 XmnI GAAnn'nnTTC Cuts at: 2286 2916 2991 3114 5540 2286 Size: 630 75 123 2426 4157 Fragments arranged by size: 4157 2426 630 123 75 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5219 276 Size: 53 83 186 936 3685 2468 Fragments arranged by size: 3685 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI