Restriction map for
pFN21AB9128
(Linear) (Six-base) MAPSORT of: KIBB9128.seq Check: 1236 from: 1 to: 2706
KIBB9128.orf
With 182 enzymes: SgfI *
September 18, 2009 17:01 ..
AccI GT'mk_AC
Cuts at: 0 1474 2706
Size: 1474 1232
Acc65I G'GTAC_C
Cuts at: 0 1188 2706
Size: 1188 1518
AclI AA'CG_TT
Cuts at: 0 115 2706
Size: 115 2591
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 599 1957 2422 2618 2706
Size: 407 192 1358 465 196 88
Fragments arranged by size:
1358 465 407 196 192 88
AflII C'TTAA_G
Cuts at: 0 1069 2706
Size: 1069 1637
AleI CACnn'nnGTG
Cuts at: 0 2512 2706
Size: 2512 194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1982 2706
Size: 1982 724
AlwNI CAG_nnn'CTG
Cuts at: 0 534 2706
Size: 534 2172
ApoI r'AATT_y
Cuts at: 0 180 281 353 586 681 984 1042 1203
Size: 180 101 72 233 95 303 58 161
Cuts at: 1203 1591 2706
Size: 388 1115
Fragments arranged by size:
1115 388 303 233 180 161 101 95
72 58
AseI AT'TA_AT
Cuts at: 0 449 2706
Size: 449 2257
BanI G'GyrC_C
Cuts at: 0 1188 1341 2706
Size: 1188 153 1365
Fragments arranged by size:
1365 1188 153
BbsI GAAGACnn'nnnn_
Cuts at: 0 904 1090 1306 2706
Size: 904 186 216 1400
Fragments arranged by size:
1400 904 216 186
BciVI GTATCCnnnnn_n'
Cuts at: 0 830 1249 2706
Size: 830 419 1457
Fragments arranged by size:
1457 830 419
BfrBI ATG'CAT
Cuts at: 0 864 1959 2706
Size: 864 1095 747
Fragments arranged by size:
1095 864 747
Bme1580I G_kGCm'C
Cuts at: 0 2048 2706
Size: 2048 658
BmgBI CAC'GTC
Cuts at: 0 2327 2706
Size: 2327 379
BmrI ACTGGGnnnn_n'
Cuts at: 0 702 2011 2706
Size: 702 1309 695
Fragments arranged by size:
1309 702 695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1365 1625 2706
Size: 1365 260 1081
Fragments arranged by size:
1365 1081 260
Bpu10I CC'TnA_GC
Cuts at: 0 1064 2706
Size: 1064 1642
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 623 1081 1632 2706
Size: 449 174 458 551 1074
Fragments arranged by size:
1074 551 458 449 174
BsaI GGTCTCn'nnnn_
Cuts at: 0 2670 2706
Size: 2670 36
BsaAI yAC'GTr
Cuts at: 0 1425 2706
Size: 1425 1281
BsiHKAI G_wGCw'C
Cuts at: 0 1635 2706
Size: 1635 1071
BsmI GAATG_Cn'
Cuts at: 0 51 1756 2706
Size: 51 1705 950
Fragments arranged by size:
1705 950 51
Bsp1286I G_dGCh'C
Cuts at: 0 1635 2048 2706
Size: 1635 413 658
Fragments arranged by size:
1635 658 413
BspHI T'CATG_A
Cuts at: 0 2672 2706
Size: 2672 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2613 2706
Size: 2613 93
BsrBI CCG'CTC
Cuts at: 0 1498 2706
Size: 1498 1208
BsrDI GCAATG_nn'
Cuts at: 0 789 1928 2706
Size: 789 1139 778
Fragments arranged by size:
1139 789 778
BstAPI GCAn_nnn'nTGC
Cuts at: 0 295 534 1918 2706
Size: 295 239 1384 788
Fragments arranged by size:
1384 788 295 239
BstBI TT'CG_AA
Cuts at: 0 1113 2706
Size: 1113 1593
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1138 2511 2706
Size: 1138 1373 195
Fragments arranged by size:
1373 1138 195
BstYI r'GATC_y
Cuts at: 0 1891 2706
Size: 1891 815
BstZ17I GTA'TAC
Cuts at: 0 1475 2706
Size: 1475 1231
BtsI GCAGTG_nn'
Cuts at: 0 1030 2706
Size: 1030 1676
DraI TTT'AAA
Cuts at: 0 1415 2193 2706
Size: 1415 778 513
Fragments arranged by size:
1415 778 513
DrdI GACnn_nn'nnGTC
Cuts at: 0 413 2706
Size: 413 2293
EarI CTCTTCn'nnn_
Cuts at: 0 1354 2441 2706
Size: 1354 1087 265
Fragments arranged by size:
1354 1087 265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 599 1365 1625 1957 2422 2618 2706
Size: 407 192 766 260 332 465 196 88
Fragments arranged by size:
766 465 407 332 260 196 192 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 877 2706
Size: 877 1829
EcoO109I rG'GnC_Cy
Cuts at: 0 851 1654 2666 2706
Size: 851 803 1012 40
Fragments arranged by size:
1012 851 803 40
EcoRI G'AATT_C
Cuts at: 0 1591 2706
Size: 1591 1115
FspI TGC'GCA
Cuts at: 0 1755 2706
Size: 1755 951
FspAI rTGC'GCAy
Cuts at: 0 1755 2706
Size: 1755 951
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 253 285 621 653 682 714 1299 1331
Size: 253 32 336 32 29 32 585 32
Cuts at: 1331 1494 1509 1526 1541 2706
Size: 163 15 17 15 1165
Fragments arranged by size:
1165 585 336 253 163 32 32 32
32 29 17 15 15
HindIII A'AGCT_T
Cuts at: 0 733 1099 2706
Size: 733 366 1607
Fragments arranged by size:
1607 733 366
KpnI G_GTAC'C
Cuts at: 0 1192 2706
Size: 1192 1514
MfeI C'AATT_G
Cuts at: 0 2531 2706
Size: 2531 175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1233 2182 2199 2666 2706
Size: 1233 949 17 467 40
Fragments arranged by size:
1233 949 467 40 17
MslI CAynn'nnrTG
Cuts at: 0 752 2512 2706
Size: 752 1760 194
Fragments arranged by size:
1760 752 194
MspA1I CmG'CkG
Cuts at: 0 334 2706
Size: 334 2372
NsiI A_TGCA'T
Cuts at: 0 866 1961 2706
Size: 866 1095 745
Fragments arranged by size:
1095 866 745
NspI r_CATG'y
Cuts at: 0 1168 1277 2706
Size: 1168 109 1429
Fragments arranged by size:
1429 1168 109
PasI CC'CwG_GG
Cuts at: 0 61 2706
Size: 61 2645
PflMI CCAn_nnn'nTGG
Cuts at: 0 1185 2706
Size: 1185 1521
PfoI T'CCnGG_A
Cuts at: 0 1664 1763 2706
Size: 1664 99 943
Fragments arranged by size:
1664 943 99
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1445 1477 2706
Size: 1445 32 1229
Fragments arranged by size:
1445 1229 32
PpuMI rG'GwC_Cy
Cuts at: 0 851 1654 2706
Size: 851 803 1052
Fragments arranged by size:
1052 851 803
PshAI GACnn'nnGTC
Cuts at: 0 2682 2706
Size: 2682 24
PsiI TTA'TAA
Cuts at: 0 1012 2473 2706
Size: 1012 1461 233
Fragments arranged by size:
1461 1012 233
PstI C_TGCA'G
Cuts at: 0 1037 2589 2706
Size: 1037 1552 117
Fragments arranged by size:
1552 1037 117
PvuII CAG'CTG
Cuts at: 0 334 2706
Size: 334 2372
ScaI AGT'ACT
Cuts at: 0 2478 2706
Size: 2478 228
SfcI C'TryA_G
Cuts at: 0 173 632 874 1033 2585 2706
Size: 173 459 242 159 1552 121
Fragments arranged by size:
1552 459 242 173 159 121
SmlI C'TyrA_G
Cuts at: 0 464 638 1069 1096 1611 2706
Size: 464 174 431 27 515 1095
Fragments arranged by size:
1095 515 464 431 174 27
SspI AAT'ATT
Cuts at: 0 1055 1702 2349 2706
Size: 1055 647 647 357
Fragments arranged by size:
1055 647 647 357
TatI w'GTAC_w
Cuts at: 0 468 2476 2706
Size: 468 2008 230
Fragments arranged by size:
2008 468 230
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2511 2706
Size: 2511 195
XmnI GAAnn'nnTTC
Cuts at: 0 281 911 986 1109 2706
Size: 281 630 75 123 1597
Fragments arranged by size:
1597 630 281 123 75
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AleI AlfI AlwNI
ApoI AseI BanI BbsI BciVI BfrBI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstBI BstXI
BstYI BstZ17I BtsI DraI DrdI EarI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI Hin4I HindIII KpnI MfeI
MmeI MslI MspA1I NsiI NspI PasI PflMI PfoI
PpiI PpuMI PshAI PsiI PstI PvuII ScaI SfcI
SmlI SspI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflIII AgeI AhdI AloI ApaI
ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanII
BbeI BbvCI BcgI BclI BglI BglII BlpI BmtI
BplI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BssSI
BstEII Bsu36I BtgI BtgZI ClaI CspCI DraIII EaeI
EagI EciI EcoICRI EcoRV FalI FseI HaeII HincII
HpaI KasI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PciI PmeI PmlI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI ZraI