Restriction map for
pFN21AB9098
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# NO CUT ENZYMES ( 72 enzymes) #
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Acc65I AccI AclI AflII AflIII
AgeI Alw44I AocI ApaI ApaLI
AscI Asp700I Asp718I AvrII BbrPI
BciVI BfrI BlnI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrGI BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI Eco81I EcoRI EcoT22I FseI
HpaI KpnI KspI MfeI MluI
MunI NcoI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SmaI SnaBI SpeI
Sse8387I SspBI SwaI XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 853 GACGTC GACGT^C
AccIII 1298 TCCGGA T^CCGGA
AhdI 2613 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2433 AGCGCT AGC^GCT
AseI 414 ATTAAT AT^TAAT
AsnI 414 ATTAAT AT^TAAT
AspEI 2613 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 713 TGCGCA TGC^GCA
BbeI 1430 GGCGCC GGCGC^C
BglII 902 AGATCT A^GATCT
BsaAI 763 TACGTG [CT]AC^GT[GA]
BseAI 1298 TCCGGA T^CCGGA
BsmBI 1285 GAGACG CGTCTC(1/5)
BspEI 1298 TCCGGA T^CCGGA
BstEII 692 GGTCACC G^GT[ACGT]ACC
BstPI 692 GGTCACC G^GT[ACGT]ACC
DraI 1642 TTTAAA TTT^AAA
DrdI 3580 GACGGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 464 CCGTGG C^C[GA][CT]GG
EagI 2654 CGGCCG C^GGCCG
Eam1105I 2613 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 151 CTCTTC CTCTTC(1/4)
EclXI 2654 CGGCCG C^GGCCG
Eco47III 2433 AGCGCT AGC^GCT
Eco52I 2654 CGGCCG C^GGCCG
EcoO65I 692 GGTCACC G^GT[ACGT]ACC
EcoRV 3612 GATATC GAT^ATC
FspI 713 TGCGCA TGC^GCA
KasI 1430 GGCGCC G^GCGCC
Ksp632I 151 CTCTTC CTCTTC(1/4)
MroI 1298 TCCGGA T^CCGGA
NarI 1430 GGCGCC GG^CGCC
NdeI 3306 CATATG CA^TATG
PaeR7I 171 CTCGAG C^TCGAG
PflMI 1740 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 414 ATTAAT AT^TAAT
SapI 150 GCTCTTC GCTCTTC(1/4)
SexAI 725 ACCAGGT A^CC[AT]GGT
SfoI 1430 GGCGCC GGC^GCC
SgrAI 2209 CGCCGGCG C[GA]^CCGG[CT]G
SspI 3689 AATATT AAT^ATT
StuI 915 AGGCCT AGG^CCT
Van91I 1740 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 171 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 1794 AAATTC [GA]^AATT[CT]
2565 AAATTC
ApoI 1794 AAATTC [GA]^AATT[CT]
2565 AAATTC
AspI 850 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2193 GACATCGTC
AvaI 171 CTCGAG C^[CT]CG[GA]G
186 CTCGGG
BamHI 11 GGATCC G^GATCC
1612 GGATCC
BcgI 1322 GCATGATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2882 GCAAGGTGATCG
BcnI 2684 CCGGG CC^[GC]GG
2919 CCCGG
BfaI 1690 CTAG C^TAG
3097 CTAG
BglI 183 GCCCTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2647 GCCTTGTCGGC
BlpI 2436 GCTCAGC GC^T[ACGT]AGC
3043 GCTGAGC
Bpu1102I 2436 GCTCAGC GC^T[ACGT]AGC
3043 GCTGAGC
BsaBI 504 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2479 GATATTGATC
BsiEI 219 CGACCG CG[GA][CT]^CG
2654 CGGCCG
BsoBI 171 CTCGAG C^[CT]CG[GA]G
186 CTCGGG
BstXI 2072 CCAGGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2760 CCAGAGGAATGG
CelII 2436 GCTCAGC GC^T[ACGT]AGC
3043 GCTGAGC
DraIII 2830 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3503 CACCCTGTG
MaeI 1690 CTAG C^TAG
3097 CTAG
MamI 504 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2479 GATATTGATC
NciI 2684 CCGGG CC^[GC]GG
2919 CCCGG
PflFI 850 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2193 GACATCGTC
SmlI 171 CTCGAG C^T[CT][GA]AG
544 CTCAAG
SphI 710 GCATGC GCATG^C
740 GCATGC
Tth111I 850 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2193 GACATCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BclI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII